{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:37:43Z","timestamp":1792611463710,"version":"4.5.0"},"reference-count":47,"publisher":"MyJove Corporation","issue":"193","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/65029","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T20:00:37Z","timestamp":1679688037000},"source":"Crossref","is-referenced-by-count":26,"title":["Assessment of Chemical Toxicity in Adult &lt;em&gt;Drosophila Melanogaster&lt;\/em&gt;"],"prefix":"10.3791","author":[{"given":"Jessica M.","family":"Holsopple","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon R.","family":"Smoot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen M.","family":"Popodi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John K.","family":"Colbourne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph R.","family":"Shaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Oliver","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C.","family":"Kaufman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason M.","family":"Tennessen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2023,3,24]]},"reference":[{"key":"ref1","type":"journal-article","article-title":"Construction industry: OSHA safety and health standards (29 CFR 1926\/1910)","year":"2022","unstructured":"United States Occupational Safety and Health Administration. Construction industry: OSHA safety and health standards (29 CFR 1926\/1910). (2022).","journal-title":"United States Occupational Safety and Health Administration"},{"key":"ref2","article-title":"Chemical, Metals, Natural Toxins & Pesticides Guidance Documents & Regulations","volume-title":"United States Food & Drug Administration","year":"2022","unstructured":"United States Food & Drug Administration. Chemical, Metals, Natural Toxins & Pesticides Guidance Documents & Regulations. at <https:\/\/www.fda.gov\/food\/guidance-documents-regulatory-information-topic-food-and-dietary-supplements\/chemical-metals-natural-toxins-pesticides-guidance-documents-regulations> (2022)."},{"key":"ref3","article-title":"Drinking Water Contaminant Candidate List (CCL) and Regulatory Determination","volume-title":"United States Food & Drug Administration","year":"2022","unstructured":"United States Food & Drug Administration. Drinking Water Contaminant Candidate List (CCL) and Regulatory Determination. at <https:\/\/www.epa.gov\/ccl> (2022)."},{"key":"ref4","article-title":"Administration Bottled Water\/Carbonated Soft Drinks Guidance Documents & Regulatory Information","volume-title":"United States Food & Drug Administration","year":"2022","unstructured":"United States Food & Drug Administration. Administration Bottled Water\/Carbonated Soft Drinks Guidance Documents & Regulatory Information. at <https:\/\/www.fda.gov\/food\/guidance-documents-regulatory-information-topic-food-and-dietary-supplements\/bottled-watercarbonated-soft-drinks-guidance-documents-regulatory-information> (2022)."},{"key":"ref5","first-page":"301","type":"journal-article","article-title":"Federal Food, Drug, and Cosmetic Act","volume":"21","year":"1934","unstructured":"United States Congress. Federal Food, Drug, and Cosmetic Act. U.S.C. 21, 301-392 (1934).","journal-title":"United States Congress"},{"key":"ref6","article-title":"Determining if a Cleaning Product is a Pesticide Under FIFRA","volume-title":"Environmental Protection Agency","year":"2022","unstructured":"United States Environmental Protection Agency. Determining if a Cleaning Product is a Pesticide Under FIFRA. at <https:\/\/www.epa.gov\/pesticide-registration\/determining-if-cleaning-product-pesticide-under-fifra> (2022)."},{"key":"ref7","first-page":"1640","type":"journal-article","article-title":"Toxic Substances Control Act of 1976. H.R. 12440","year":"1976","unstructured":"United States Congress. Toxic Substances Control Act of 1976. H.R.12440. 1640-1707. Library of Congress, United States. (1976).","journal-title":"Library of Congress, United States"},{"issue":"5","key":"ref8","doi-asserted-by":"crossref","first-page":"2575","DOI":"10.1021\/acs.est.9b06379","type":"journal-article","article-title":"Toward a global understanding of chemical pollution: a first comprehensive analysis of national and regional chemical inventories","volume":"54","author":"Wang","year":"2020","unstructured":"Wang, Z., Walker, G. W., Muir, D. C. G., Nagatani-Yoshida, K. Toward a global understanding of chemical pollution: a first comprehensive analysis of national and regional chemical inventories. Environmental Science Technology. 54 (5), 2575-2584 (2020).","journal-title":"Environmental Science Technology"},{"issue":"2","key":"ref9","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1124\/pr.110.003293","type":"journal-article","article-title":"Human disease models in Drosophila melanogaster and the role of the fly in therapeutic drug discovery","volume":"63","author":"Pandey","year":"2011","unstructured":"Pandey, U. B., Nichols, C. D. Human disease models in Drosophila melanogaster and the role of the fly in therapeutic drug discovery. Pharmacological Reviews. 63 (2), 411-436 (2011).","journal-title":"Pharmacological Reviews"},{"key":"ref10","article-title":"New Approach Methods Work Plan","volume-title":"United States Environmental Protection Agency","year":"2021","unstructured":"United States Environmental Protection Agency. New Approach Methods Work Plan. at <https:\/\/www.epa.gov\/system\/files\/documents\/2021-11\/nams-work-plan_11_15_21_508-tagged.pdf> (2021)."},{"issue":"3","key":"ref11","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.ejpb.2010.12.027","type":"journal-article","article-title":"From alternative methods to a new toxicology","volume":"77","author":"Hartung","year":"2011","unstructured":"Hartung, T. From alternative methods to a new toxicology. European Journal of Pharmaceutics and Biopharmaceutics. 77 (3), 338-349 (2011).","journal-title":"European Journal of Pharmaceutics and Biopharmaceutics"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.comtox.2019.01.003","type":"journal-article","article-title":"Building confidence in non-animal methods: Practical examples of collaboration between regulators, researchers and industry","volume":"10","author":"Mahony","year":"2019","unstructured":"Mahony, C. Building confidence in non-animal methods: Practical examples of collaboration between regulators, researchers and industry. Computational Toxicology. 10, 78-80 (2019).","journal-title":"Computational Toxicology"},{"issue":"5","key":"ref13","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1021\/acs.chemrestox.7b00339","type":"journal-article","article-title":"Accelerating the pace of chemical risk assessment","volume":"31","author":"Kavlock","year":"2018","unstructured":"Kavlock, R. J. et al. Accelerating the pace of chemical risk assessment. Chemical Research in Toxicology. 31 (5), 287-290 (2018).","journal-title":"Chemical Research in Toxicology"},{"key":"ref14","type":"journal-article","article-title":"New Approach Methodologies in Regulatory Science, Proceedings of the Scientific Workshop","year":"2016","unstructured":"European Chemicals Agency. New Approach Methodologies in Regulatory Science, Proceedings of the Scientific Workshop: Helsinki, Finland, 19-20 April 2016. doi\/10.2823\/543644. European Chemicals Agency. (2016).","journal-title":"European Chemicals Agency"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.cotox.2019.07.001","type":"journal-article","article-title":"New approach methodologies for exposure science","volume":"15","author":"Wambaugh","year":"2019","unstructured":"Wambaugh, J. F. et al. New approach methodologies for exposure science. Current Opinion in Toxicology. 15, 76-92 (2019).","journal-title":"Current Opinion in Toxicology"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.ntt.2009.06.004","type":"journal-article","article-title":"Drosophotoxicology: The growing potential for Drosophila in neurotoxicology","volume":"32","author":"Rand","year":"2010","unstructured":"Rand, M. D. Drosophotoxicology: The growing potential for Drosophila in neurotoxicology. Neurotoxicology and Teratology. 32 (1), 74-83 (2010).","journal-title":"Neurotoxicology and Teratology"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"396","DOI":"10.3109\/17435390.2014.940405","type":"journal-article","article-title":"Drosophila melanogaster as a model organism to study nanotoxicity","volume":"9","author":"Ong","year":"2015","unstructured":"Ong, C., Yung, L. Y. L., Cai, Y., Bay, B. H., Baeg, G. H. Drosophila melanogaster as a model organism to study nanotoxicity. Nanotoxicology. 9 (3), 396-403 (2015).","journal-title":"Nanotoxicology"},{"issue":"137","key":"ref18","first-page":"e57450","type":"journal-article","article-title":"Experimental protocol for using Drosophila as an invertebrate model system for toxicity testing in the laboratory","author":"Peterson","year":"2018","unstructured":"Peterson, E. K., Long, H. E. Experimental protocol for using Drosophila as an invertebrate model system for toxicity testing in the laboratory. Journal of Visualized Experiments. (137), e57450 (2018).","journal-title":"Journal of Visualized Experiments"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1002\/0471140856.tx0112s59","type":"journal-article","article-title":"Developmental toxicity assays using the Drosophila model","volume":"59","author":"Rand","year":"2014","unstructured":"Rand, M. D., Montgomery, S. L., Prince, L., Vorojeikina, D. Developmental toxicity assays using the Drosophila model. Current Protocols in Toxicology. 59, 1.12.11-20 (2014).","journal-title":"Current Protocols in Toxicology"},{"issue":"17","key":"ref20","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1101\/gad.17280911","type":"journal-article","article-title":"Transcriptional regulation of xenobiotic detoxification in Drosophila","volume":"25","author":"Misra","year":"2011","unstructured":"Misra, J. R., Horner, M. A., Lam, G., Thummel, C. S. Transcriptional regulation of xenobiotic detoxification in Drosophila. Genes & Development. 25 (17), 1796-1806 (2011).","journal-title":"Genes & Development"},{"issue":"1","key":"ref21","first-page":"33","type":"journal-article","article-title":"Drosophila melanogaster as a promising model organism in toxicological studies","volume":"1","author":"Rocha","year":"2013","unstructured":"Rocha, J. B. T. Drosophila melanogaster as a promising model organism in toxicological studies. Archives of Basic and Applied Medicine. 1 (1), 33-38 (2013).","journal-title":"Archives of Basic and Applied Medicine"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/978-1-4939-9182-2_10","type":"journal-article","article-title":"Drosophila as a model for developmental toxicology: using and extending the drosophotoxicology model","volume":"1965","author":"Affleck","year":"2019","unstructured":"Affleck, J. G., Walker, V. K. Drosophila as a model for developmental toxicology: using and extending the drosophotoxicology model. Methods in Molecular Biology. 1965, 139-153 (2019).","journal-title":"Methods in Molecular Biology"},{"issue":"3","key":"ref23","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1242\/dmm.023762","type":"journal-article","article-title":"Drosophila tools and assays for the study of human diseases","volume":"9","author":"Ugur","year":"2016","unstructured":"Ugur, B., Chen, K., Bellen, H. J. Drosophila tools and assays for the study of human diseases. Disease Models & Mechanisms. 9 (3), 235-244 (2016).","journal-title":"Disease Models & Mechanisms"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1093\/nar\/30.1.149","type":"journal-article","article-title":"Homophila: human disease gene cognates in Drosophila","volume":"30","author":"Chien","year":"2002","unstructured":"Chien, S., Reiter, L. T., Bier, E., Gribskov, M. Homophila: human disease gene cognates in Drosophila. Nucleic Acids Research. 30 (1), 149-151 (2002).","journal-title":"Nucleic Acids Research"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1534\/genetics.117.203067","type":"journal-article","article-title":"Model organisms facilitate rare disease diagnosis and therapeutic research","volume":"207","author":"Wangler","year":"2017","unstructured":"Wangler, M. F. et al. Model organisms facilitate rare disease diagnosis and therapeutic research. Genetics. 207 (1), 9-27 (2017).","journal-title":"Genetics"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"100287","DOI":"10.1016\/j.envadv.2022.100287","type":"journal-article","article-title":"Toxicity by descent: A comparative approach for chemical hazard assessment","volume":"9","author":"Colbourne","year":"2022","unstructured":"Colbourne, J. K. et al. Toxicity by descent: A comparative approach for chemical hazard assessment. Environmental Advances. 9, 100287 (2022).","journal-title":"Environmental Advances"},{"issue":"3","key":"ref27","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1534\/genetics.115.183392","type":"journal-article","article-title":"Genetics on the fly: a primer on the Drosophila model system","volume":"201","author":"Hales","year":"2015","unstructured":"Hales, K. G., Korey, C. A., Larracuente, A. M., Roberts, D. M. Genetics on the fly: a primer on the Drosophila model system. Genetics. 201 (3), 815-842 (2015).","journal-title":"Genetics"},{"key":"ref28","volume-title":"Drosophila Workers Unite! A Laboratory Manual for Working with Drosophila","author":"Markstein","year":"2018","unstructured":"Markstein, M. Drosophila Workers Unite! A Laboratory Manual for Working with Drosophila. at <http:\/\/marksteinlab.org\/wp-content\/uploads\/2019\/01\/MicheleMarkstein-DrosophilaWorkersUnite-PREPRINT-JAN2019.pdf>. Michele Markstein (2018)."},{"issue":"5","key":"ref29","doi-asserted-by":"crossref","first-page":"37495","DOI":"10.1371\/journal.pone.0037495","type":"journal-article","article-title":"Simple ways to measure behavioral responses of Drosophila to stimuli and use of these methods to characterize a novel mutant","volume":"7","author":"Vang","year":"2012","unstructured":"Vang, L. L., Medvedev, A. V., Adler, J. Simple ways to measure behavioral responses of Drosophila to stimuli and use of these methods to characterize a novel mutant. PLoS One. 7 (5), e37495 (2012).","journal-title":"PLoS One"},{"issue":"61","key":"ref30","first-page":"e3795","type":"journal-article","article-title":"Methods to assay Drosophila behavior","author":"Nichols","year":"2012","unstructured":"Nichols, C. D., Becnel, J., Pandey, U. B., Byfield, F. Methods to assay Drosophila behavior. Journal of Visualized Experiments. (61), e3795 (2012).","journal-title":"Journal of Visualized Experiments"},{"key":"ref31","article-title":"Bloomington Drosophilia Stock Center","volume-title":"BDSC Cornmeal Food","year":"2022","unstructured":"Bloomington Drosophila Stock Center. BDSC Cornmeal Food. at <https:\/\/bdsc.indiana.edu\/information\/recipes\/bloomfood.html> (2022)."},{"issue":"3","key":"ref32","doi-asserted-by":"crossref","first-page":"2722","DOI":"10.21769\/BioProtoc.2722","type":"journal-article","article-title":"How to catch a Smurf? - ageing and beyond\u2026 in vivo assessment of intestinal permeability in multiple model organisms","volume":"8","author":"Martins","year":"2018","unstructured":"Martins, R. R., McCracken, A. W., Simons, M. J. P., Henriques, C. M., Rera, M. How to catch a Smurf? - ageing and beyond\u2026 in vivo assessment of intestinal permeability in multiple model organisms. Bio-Protocol. 8 (3), e2722 (2018).","journal-title":"Bio-Protocol"},{"issue":"5","key":"ref33","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/j.cmet.2011.09.013","type":"journal-article","article-title":"Modulation of longevity and tissue homeostasis by the Drosophila PGC-1 homolog","volume":"14","author":"Rera","year":"2011","unstructured":"Rera, M. et al. Modulation of longevity and tissue homeostasis by the Drosophila PGC-1 homolog. Cell Metabolism. 14 (5), 623-634 (2011).","journal-title":"Cell Metabolism"},{"key":"ref34","article-title":"Benchmark Dose Software (BMDS) (Build 3.3; Model Library Version 2022.10) [Computer Software]","volume-title":"United States Environmental Protection Agency","year":"2022","unstructured":"United States Environmental Protection Agency. Benchmark Dose Software (BMDS) (Build 3.3; Model Library Version 2022.10) [Computer Software]. Available from https:\/\/www.epa.gov\/bmds\/download-bmds. (2022)."},{"issue":"2","key":"ref35","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1093\/toxsci\/kfn192","type":"journal-article","article-title":"Investigating arsenic susceptibility from a genetic perspective in Drosophila reveals a key role for glutathione synthetase","volume":"107","author":"Ortiz","year":"2009","unstructured":"Ortiz, J. G., Opoka, R., Kane, D., Cartwright, I. L. Investigating arsenic susceptibility from a genetic perspective in Drosophila reveals a key role for glutathione synthetase. Toxicological Sciences. 107 (2), 416-426 (2009).","journal-title":"Toxicological Sciences"},{"issue":"3566","key":"ref36","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1126\/science.140.3566.493","type":"journal-article","article-title":"Arsenates: effect on fecundity in some Diptera","volume":"140","author":"Pickett","year":"1963","unstructured":"Pickett, A. D., Patterson, N. A. Arsenates: effect on fecundity in some Diptera. Science. 140 (3566), 493-494 (1963).","journal-title":"Science"},{"issue":"2","key":"ref37","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1007\/BF01699411","type":"journal-article","article-title":"Differential effects of arsenite and arsenate to Drosophila melanogaster in a combined adult\/developmental toxicity assay","volume":"42","author":"Goldstein","year":"1989","unstructured":"Goldstein, S. H., Babich, H. Differential effects of arsenite and arsenate to Drosophila melanogaster in a combined adult\/developmental toxicity assay. Bulletin of Environmental Contamination and Toxicology. 42 (2), 276-282 (1989).","journal-title":"Bulletin of Environmental Contamination and Toxicology"},{"issue":"1","key":"ref38","first-page":"1928","type":"journal-article","article-title":"Response of fluctuating asymmetry to arsenic toxicity: support for the developmental selection hypothesis","volume":"118","author":"Polak","year":"1928","unstructured":"Polak, M., Opoka, R., Cartwright, I. L. Response of fluctuating asymmetry to arsenic toxicity: support for the developmental selection hypothesis. Environmental Pollution. 118 (1), 1928 (2002).","journal-title":"Environmental Pollution"},{"issue":"7","key":"ref39","doi-asserted-by":"crossref","first-page":"1006907","DOI":"10.1371\/journal.pgen.1006907","type":"journal-article","article-title":"A Drosophila model for toxicogenomics: Genetic variation in susceptibility to heavy metal exposure","volume":"13","author":"Zhou","year":"2017","unstructured":"Zhou, S. et al. A Drosophila model for toxicogenomics: Genetic variation in susceptibility to heavy metal exposure. PLoS Genetics. 13 (7), e1006907 (2017).","journal-title":"PLoS Genetics"},{"issue":"1","key":"ref40","doi-asserted-by":"crossref","first-page":"100799","DOI":"10.1016\/j.isci.2019.100799","type":"journal-article","article-title":"Chemosensation and evolution of Drosophila host plant selection","volume":"23","author":"Anholt","year":"2020","unstructured":"Anholt, R. R. H. Chemosensation and evolution of Drosophila host plant selection. iScience. 23 (1), 100799 (2020).","journal-title":"iScience"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"41","DOI":"10.3389\/fevo.2015.00041","type":"journal-article","article-title":"Chemicals and chemoreceptors: ecologically relevant signals driving behavior in Drosophila","volume":"3","author":"Depetris-Chauvin","year":"2015","unstructured":"Depetris-Chauvin, A., Galagovsky, D., Grosjean, Y. Chemicals and chemoreceptors: ecologically relevant signals driving behavior in Drosophila. Frontiers in Ecology and Evolution. 3, 41 (2015).","journal-title":"Frontiers in Ecology and Evolution"},{"issue":"2","key":"ref42","doi-asserted-by":"crossref","first-page":"101410","DOI":"10.1016\/j.xpro.2022.101410","type":"journal-article","article-title":"Protocol for binary food choice assays using Drosophila melanogaster","volume":"3","author":"Aryal","year":"2022","unstructured":"Aryal, B. et al. Protocol for binary food choice assays using Drosophila melanogaster. STAR Protocols. 3 (2), 101410 (2022).","journal-title":"STAR Protocols"},{"issue":"3","key":"ref43","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1093\/chemse\/12.3.481","type":"journal-article","article-title":"Acute differential sensitivity and role of the central nervous system in the feeding behavior of Drosophila melanogaster","volume":"12","author":"Shimada","year":"1987","unstructured":"Shimada, I., Nakao, M., Kawazoe, Y. Acute differential sensitivity and role of the central nervous system in the feeding behavior of Drosophila melanogaster. Chemical Senses. 12 (3), 481-490 (1987).","journal-title":"Chemical Senses"},{"issue":"4","key":"ref44","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1007\/BF00612007","type":"journal-article","article-title":"Genetic dimorphism in the taste sensitivity to trehalose in Drosophila melanogaster","volume":"147","author":"Tanimura","year":"1982","unstructured":"Tanimura, T., Isono, K., Takamura, T., Shimada, I. Genetic dimorphism in the taste sensitivity to trehalose in Drosophila melanogaster. Journal of Comparative Physiology. 147 (4), 433-437 (1982).","journal-title":"Journal of Comparative Physiology"},{"issue":"6","key":"ref45","doi-asserted-by":"crossref","first-page":"101107","DOI":"10.1371\/journal.pone.0101107","type":"journal-article","article-title":"FLIC: high-throughput, continuous analysis of feeding behaviors in Drosophila","volume":"9","author":"Ro","year":"2014","unstructured":"Ro, J., Harvanek, Z. M., Pletcher, S. D. FLIC: high-throughput, continuous analysis of feeding behaviors in Drosophila. PLoS One. 9 (6), e101107 (2014).","journal-title":"PLoS One"},{"key":"ref46","type":"book","article-title":"Toward Precision Medicine: Building a Knowledge Network for Biomedical Research and a New Taxonomy of Disease","volume-title":"National Academy of Sciences","year":"2011","unstructured":"National Research Council. Toward Precision Medicine: Building a Knowledge Network for Biomedical Research and a New Taxonomy of Disease. National Academies Press (US) Copyright \u00a9 2011, National Academy of Sciences. (2011)."},{"issue":"136","key":"ref47","first-page":"e57847","type":"journal-article","article-title":"Preparation of Drosophila larval samples for gas chromatography-mass spectrometry (GC-MS)-based metabolomics","author":"Li","year":"2018","unstructured":"Li, H., Tennessen, J. M. Preparation of Drosophila larval samples for gas chromatography-mass spectrometry (GC-MS)-based metabolomics. Journal of Visualized Experiments. (136), e57847 (2018).","journal-title":"Journal of Visualized Experiments"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/65029\/assessment-of-chemical-toxicity-in-adult-drosophila-melanogaster","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:37:47Z","timestamp":1792607867000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/65029"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,24]]},"references-count":47,"journal-issue":{"issue":"193","published-online":{"date-parts":[[2023]]}},"URL":"https:\/\/doi.org\/10.3791\/65029","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,24]]}}}