{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:43:39Z","timestamp":1792608219666,"version":"4.5.0"},"reference-count":38,"publisher":"MyJove Corporation","issue":"194","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/65254","type":"journal-article","created":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T20:03:25Z","timestamp":1682107405000},"source":"Crossref","is-referenced-by-count":1,"title":["Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining"],"prefix":"10.3791","author":[{"given":"Dillon","family":"Strepay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafal","family":"Olszewski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Taukulis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Dixon","family":"Johns","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoujun","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Hoa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2023,4,21]]},"reference":[{"issue":"1023","key":"ref1","first-page":"227","type":"journal-article","article-title":"Observations on the electrochemistry of the cochlear endolymph of the rat: a quantitative study of its electrical potential and ionic composition as determined by means of flame spectrophotometry","volume":"171","author":"Bosher","year":"1968","unstructured":"Bosher, S. K., Warren, R. L. Observations on the electrochemistry of the cochlear endolymph of the rat: a quantitative study of its electrical potential and ionic composition as determined by means of flame spectrophotometry. Proceedings of the Royal Society of London. Series B. Biological Sciences. 171 (1023), 227-247 (1968).","journal-title":"Proceedings of the Royal Society of London. Series B. Biological Sciences"},{"issue":"1-2","key":"ref2","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.heares.2011.01.010","type":"journal-article","article-title":"Ion flow in stria vascularis and the production and regulation of cochlear endolymph and the endolymphatic potential","volume":"277","author":"Patuzzi","year":"2011","unstructured":"Patuzzi, R. Ion flow in stria vascularis and the production and regulation of cochlear endolymph and the endolymphatic potential. Hearing Research. 277 (1-2), 4-19 (2011).","journal-title":"Hearing Research"},{"issue":"1-2","key":"ref3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-5955(02)00279-4","type":"journal-article","article-title":"K+ cycling and the endocochlear potential","volume":"165","author":"Wangemann","year":"2002","unstructured":"Wangemann, P. K+ cycling and the endocochlear potential. Hearing Research. 165 (1-2), 1-9 (2002).","journal-title":"Hearing Research"},{"issue":"18","key":"ref4","doi-asserted-by":"crossref","first-page":"4459","DOI":"10.1113\/jphysiol.2013.258046","type":"journal-article","article-title":"The mechanism underlying maintenance of the endocochlear potential by the K+ transport system in fibrocytes of the inner ear","volume":"591","author":"Adachi","year":"2013","unstructured":"Adachi, N. et al. The mechanism underlying maintenance of the endocochlear potential by the K+ transport system in fibrocytes of the inner ear. The Journal of Physiology. 591 (18), 4459-4472 (2013).","journal-title":"The Journal of Physiology"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/s00424-009-0754-z","type":"journal-article","article-title":"How is the highly positive endocochlear potential formed? The specific architecture of the stria vascularis and the roles of the ion-transport apparatus","volume":"459","author":"Hibino","year":"2010","unstructured":"Hibino, H., Nin, F., Tsuzuki, C., Kurachi, Y. How is the highly positive endocochlear potential formed? The specific architecture of the stria vascularis and the roles of the ion-transport apparatus. Pflugers Archiv. 459 (4), 521-533 (2010).","journal-title":"Pflugers Archiv"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"C1187","DOI":"10.1152\/ajpcell.00024.2007","type":"journal-article","article-title":"Functional significance of channels and transporters expressed in the inner ear and kidney","volume":"293","author":"Lang","year":"2007","unstructured":"Lang, F., Vallon, V., Knipper, M., Wangemann, P. Functional significance of channels and transporters expressed in the inner ear and kidney. American Journal of Physiology. Cell Physiology. 293 (4), C1187-C1208 (2007).","journal-title":"American Journal of Physiology. Cell Physiology"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"239","DOI":"10.3389\/fnmol.2017.00239","type":"journal-article","article-title":"The human "cochlear battery"-claudin-11 barrier and ion transport proteins in the lateral wall of the cochlea","volume":"10","author":"Liu","year":"2017","unstructured":"Liu, W., Schrott-Fischer, A., Glueckert, R., Benav, H., Rask-Andersen, H. The human \"cochlear battery\"-claudin-11 barrier and ion transport proteins in the lateral wall of the cochlea. Frontiers in Molecular Neuroscience. 10, 239 (2017).","journal-title":"Frontiers in Molecular Neuroscience"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"C403","DOI":"10.1152\/ajpcell.00312.2001","type":"journal-article","article-title":"KCNJ10 (Kir4.1) potassium channel knockout abolishes endocochlear potential","volume":"282","author":"Marcus","year":"2002","unstructured":"Marcus, D. C., Wu, T., Wangemann, P., Kofuji, P. KCNJ10 (Kir4.1) potassium channel knockout abolishes endocochlear potential. American Journal of Physiology. Cell Physiology. 282 (2), C403-C407 (2002).","journal-title":"American Journal of Physiology. Cell Physiology"},{"issue":"1-2","key":"ref9","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/0378-5955(91)90153-Z","type":"journal-article","article-title":"Differentiation of inner ear fibrocytes according to their ion transport related activity","volume":"56","author":"Spicer","year":"1991","unstructured":"Spicer, S. S., Schulte, B. A. Differentiation of inner ear fibrocytes according to their ion transport related activity. Hearing Research. 56 (1-2), 53-64 (1991).","journal-title":"Hearing Research"},{"issue":"1-2","key":"ref10","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0378-5955(95)00009-S","type":"journal-article","article-title":"Ion transport mechanisms responsible for K+ secretion and the transepithelial voltage across marginal cells of stria vascularis in vitro","volume":"84","author":"Wangemann","year":"1995","unstructured":"Wangemann, P., Liu, J., Marcus, D. C. Ion transport mechanisms responsible for K+ secretion and the transepithelial voltage across marginal cells of stria vascularis in vitro. Hearing Research. 84 (1-2), 19-29 (1995).","journal-title":"Hearing Research"},{"issue":"9","key":"ref11","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.1007\/s00424-016-1853-2","type":"journal-article","article-title":"The unique ion permeability profile of cochlear fibrocytes and its contribution to establishing their positive resting membrane potential","volume":"468","author":"Yoshida","year":"2016","unstructured":"Yoshida, T. et al. The unique ion permeability profile of cochlear fibrocytes and its contribution to establishing their positive resting membrane potential. Pflugers Archiv. 468 (9), 1609-1619 (2016).","journal-title":"Pflugers Archiv"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"108682","DOI":"10.1016\/j.heares.2022.108682","type":"journal-article","article-title":"The role of the stria vascularis in neglected otologic disease","volume":"428","author":"Johns","year":"2023","unstructured":"Johns, J. D., Adadey, S. M., Hoa, M. The role of the stria vascularis in neglected otologic disease. Hearing Research. 428, 108682 (2023).","journal-title":"Hearing Research"},{"issue":"9","key":"ref13","doi-asserted-by":"crossref","first-page":"e2117846119","DOI":"10.1073\/pnas.2117946119","type":"journal-article","article-title":"In vivo real-time imaging reveals megalin as the aminoglycoside gentamicin transporter into cochlea whose inhibition is otoprotective","volume":"119","author":"Kim","year":"2022","unstructured":"Kim, J., Ricci, A. J. In vivo real-time imaging reveals megalin as the aminoglycoside gentamicin transporter into cochlea whose inhibition is otoprotective. Proceedings of the National Academy of Sciences. 119 (9), e2117946119 (2022).","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1152\/physiol.00018.2009","type":"journal-article","article-title":"Potassium ion movement in the inner ear: insights from genetic disease and mouse models","volume":"24","author":"Zdebik","year":"2009","unstructured":"Zdebik, A. A., Wangemann, P., Jentsch, T. J. Potassium ion movement in the inner ear: insights from genetic disease and mouse models. Physiology. 24, 307-316 (2009).","journal-title":"Physiology"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.neuroscience.2014.01.036","type":"journal-article","article-title":"The role of an inwardly rectifying K+ channel (Kir4.1) in the inner ear and hearing loss","volume":"265","author":"Chen","year":"2014","unstructured":"Chen, J., Zhao, H. B. The role of an inwardly rectifying K+ channel (Kir4.1) in the inner ear and hearing loss. Neuroscience. 265, 137-146 (2014).","journal-title":"Neuroscience"},{"issue":"3","key":"ref16","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1242\/dev.107.3.453","type":"journal-article","article-title":"Another role for melanocytes: their importance for normal stria vascularis development in the mammalian inner ear","volume":"107","author":"Steel","year":"1989","unstructured":"Steel, K. P., Barkway, C. Another role for melanocytes: their importance for normal stria vascularis development in the mammalian inner ear. Development. 107 (3), 453-463 (1989).","journal-title":"Development"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.neuroscience.2015.09.016","type":"journal-article","article-title":"Progressive irreversible hearing loss is caused by stria vascularis degeneration in an Slc26a4-insufficient mouse model of large vestibular aqueduct syndrome","volume":"310","author":"Ito","year":"2015","unstructured":"Ito, T., Nishio, A., Wangemann, P., Griffith, A. J. Progressive irreversible hearing loss is caused by stria vascularis degeneration in an Slc26a4-insufficient mouse model of large vestibular aqueduct syndrome. Neuroscience. 310, 188-197 (2015).","journal-title":"Neuroscience"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"18100","DOI":"10.1038\/s41598-020-75238-8","type":"journal-article","article-title":"Characterization of rare spindle and root cell transcriptional profiles in the stria vascularis of the adult mouse cochlea","volume":"10","author":"Gu","year":"2020","unstructured":"Gu, S. et al. Characterization of rare spindle and root cell transcriptional profiles in the stria vascularis of the adult mouse cochlea. Scientific Reports. 10 (1), 18100 (2020).","journal-title":"Scientific Reports"},{"issue":"32","key":"ref19","doi-asserted-by":"crossref","first-page":"7051","DOI":"10.1523\/JNEUROSCI.1640-04.2004","type":"journal-article","article-title":"Deafness in claudin 11-null mice reveals the critical contribution of basal cell tight junctions to stria vascularis function","volume":"24","author":"Gow","year":"2004","unstructured":"Gow, A. et al. Deafness in claudin 11-null mice reveals the critical contribution of basal cell tight junctions to stria vascularis function. The Journal of Neuroscience. 24 (32), 7051-7062 (2004).","journal-title":"The Journal of Neuroscience"},{"issue":"8","key":"ref20","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.15252\/emmm.201404929","type":"journal-article","article-title":"Virally mediated Kcnq1 gene replacement therapy in the immature scala media restores hearing in a mouse model of human Jervell and Lange-Nielsen deafness syndrome","volume":"7","author":"Chang","year":"2015","unstructured":"Chang, Q. et al. Virally mediated Kcnq1 gene replacement therapy in the immature scala media restores hearing in a mouse model of human Jervell and Lange-Nielsen deafness syndrome. EMBO Molecular Medicine. 7 (8), 1077-1086 (2015).","journal-title":"EMBO Molecular Medicine"},{"issue":"2","key":"ref21","first-page":"162","type":"journal-article","article-title":"Mutational and phenotypic spectra of KCNE1 deficiency in Jervell and Lange-Nielsen Syndrome and Romano-Ward Syndrome","volume":"40","author":"Faridi","year":"2019","unstructured":"Faridi, R. et al. Mutational and phenotypic spectra of KCNE1 deficiency in Jervell and Lange-Nielsen Syndrome and Romano-Ward Syndrome. Human Mutation. 40 (2), 162-176 (2019).","journal-title":"Human Mutation"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1741-7015-2-30","type":"journal-article","article-title":"Loss of KCNJ10 protein expression abolishes endocochlear potential and causes deafness in Pendred syndrome mouse model","volume":"2","author":"Wangemann","year":"2004","unstructured":"Wangemann, P. et al. Loss of KCNJ10 protein expression abolishes endocochlear potential and causes deafness in Pendred syndrome mouse model. BMC Medicine. 2, 30 (2004).","journal-title":"BMC Medicine"},{"issue":"1-2","key":"ref23","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0378-5955(03)00338-1","type":"journal-article","article-title":"Expression patterns of claudins, tight junction adhesion molecules, in the inner ear","volume":"187","author":"Kitajiri","year":"2004","unstructured":"Kitajiri, S.-I. et al. Expression patterns of claudins, tight junction adhesion molecules, in the inner ear. Hearing Research. 187 (1-2), 25-34 (2004).","journal-title":"Hearing Research"},{"issue":"3","key":"ref24","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1007\/s10162-010-0218-3","type":"journal-article","article-title":"The membrane properties of cochlear root cells are consistent with roles in potassium recirculation and spatial buffering","volume":"11","author":"Jagger","year":"2010","unstructured":"Jagger, D. J., Nevill, G., Forge, A. The membrane properties of cochlear root cells are consistent with roles in potassium recirculation and spatial buffering. Journal of the Association for Research in Otolaryngology. 11 (3), 435-448 (2010).","journal-title":"Journal of the Association for Research in Otolaryngology"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"818395","DOI":"10.3389\/fneur.2022.818395","type":"journal-article","article-title":"Tissue-resident macrophages in the stria vascularis","volume":"13","author":"Ito","year":"2022","unstructured":"Ito, T., Kurata, N., Fukunaga, Y. Tissue-resident macrophages in the stria vascularis. Frontiers in Neurology. 13, 818395 (2022).","journal-title":"Frontiers in Neurology"},{"issue":"8","key":"ref26","doi-asserted-by":"crossref","first-page":"e143285","DOI":"10.1172\/jci.insight.143285","type":"journal-article","article-title":"VEGFA165 gene therapy ameliorates blood-labyrinth barrier breakdown and hearing loss","volume":"6","author":"Zhang","year":"2021","unstructured":"Zhang, J. et al. VEGFA165 gene therapy ameliorates blood-labyrinth barrier breakdown and hearing loss. JCI Insight. 6 (8), e143285 (2021).","journal-title":"JCI Insight"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.heares.2016.01.010","type":"journal-article","article-title":"Pathophysiology of the cochlear intrastrial fluid-blood barrier (review)","volume":"338","author":"Shi","year":"2016","unstructured":"Shi, X. Pathophysiology of the cochlear intrastrial fluid-blood barrier (review). Hearing Research. 338, 52-63 (2016).","journal-title":"Hearing Research"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"630561","DOI":"10.3389\/fneur.2021.630561","type":"journal-article","article-title":"Identification of potential Meniere's disease targets in the adult stria vascularis","volume":"12","author":"Gu","year":"2021","unstructured":"Gu, S. et al. Identification of potential Meniere's disease targets in the adult stria vascularis. Frontiers in Neurology. 12, 630561 (2021).","journal-title":"Frontiers in Neurology"},{"issue":"5","key":"ref29","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1042\/ETLS20210074","type":"journal-article","article-title":"Single nucleus RNA-sequencing: how it's done, applications and limitations","volume":"5","author":"Fischer","year":"2021","unstructured":"Fischer, J., Ayers, T. Single nucleus RNA-sequencing: how it's done, applications and limitations. Emerging Topics in Life Sciences. 5 (5), 687-690 (2021).","journal-title":"Emerging Topics in Life Sciences"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"316","DOI":"10.3389\/fnmol.2019.00316","type":"journal-article","article-title":"Single cell and single nucleus RNA-Seq reveal cellular heterogeneity and homeostatic regulatory networks in adult mouse stria vascularis","volume":"12","author":"Korrapati","year":"2019","unstructured":"Korrapati, S. et al. Single cell and single nucleus RNA-Seq reveal cellular heterogeneity and homeostatic regulatory networks in adult mouse stria vascularis. Frontiers in Molecular Neuroscience. 12, 316 (2019).","journal-title":"Frontiers in Molecular Neuroscience"},{"issue":"3","key":"ref31","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1002\/lio2.388","type":"journal-article","article-title":"Applications of single-cell sequencing for the field of otolaryngology: A contemporary review","volume":"5","author":"Pyle","year":"2020","unstructured":"Pyle, M. P., Hoa, M. Applications of single-cell sequencing for the field of otolaryngology: A contemporary review. Laryngoscope Investigative Otolaryngology. 5 (3), 404-431 (2020).","journal-title":"Laryngoscope Investigative Otolaryngology"},{"issue":"8","key":"ref32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s12276-018-0071-8","type":"journal-article","article-title":"Single-cell RNA sequencing technologies and bioinformatics pipelines","volume":"50","author":"Hwang","year":"2018","unstructured":"Hwang, B., Lee, J. H., Bang, D. Single-cell RNA sequencing technologies and bioinformatics pipelines. Experimental & Molecular Medicine. 50 (8), 1-14 (2018).","journal-title":"Experimental & Molecular Medicine"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"175","DOI":"10.3389\/fcell.2019.00175","type":"journal-article","article-title":"Cross-species analysis of single-cell transcriptomic data","volume":"7","author":"Shafer","year":"2019","unstructured":"Shafer, M. E. R. Cross-species analysis of single-cell transcriptomic data. Frontiers in Cell and Developmental Biology. 7, 175 (2019).","journal-title":"Frontiers in Cell and Developmental Biology"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"317","DOI":"10.3389\/fgene.2019.00317","type":"journal-article","article-title":"Single-cell RNA-Seq technologies and related computational data analysis","volume":"10","author":"Chen","year":"2019","unstructured":"Chen, G., Ning, B., Shi, T. Single-cell RNA-Seq technologies and related computational data analysis. Frontiers in Genetics. 10, 317 (2019).","journal-title":"Frontiers in Genetics"},{"issue":"10","key":"ref35","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1038\/s41576-021-00370-8","type":"journal-article","article-title":"Integrating single-cell and spatial transcriptomics to elucidate intercellular tissue dynamics","volume":"22","author":"Longo","year":"2021","unstructured":"Longo, S. K., Guo, M. G., Ji, A. L., Khavari, P. A. Integrating single-cell and spatial transcriptomics to elucidate intercellular tissue dynamics. Nature Reviews Genetics. 22 (10), 627-644 (2021).","journal-title":"Nature Reviews Genetics"},{"issue":"1","key":"ref36","doi-asserted-by":"crossref","first-page":"52","DOI":"10.4132\/jptm.2022.12.19","type":"journal-article","article-title":"Perspectives on single-nucleus RNA sequencing in different cell types and tissues","volume":"57","author":"Kim","year":"2023","unstructured":"Kim, N., Kang, H., Jo, A., Yoo, S. A., Lee, H. O. Perspectives on single-nucleus RNA sequencing in different cell types and tissues. Journal of Pathology and Translational Medicine. 57 (1), 52-59 (2023).","journal-title":"Journal of Pathology and Translational Medicine"},{"issue":"49","key":"ref37","doi-asserted-by":"crossref","first-page":"19802","DOI":"10.1073\/pnas.1319700110","type":"journal-article","article-title":"RNA-sequencing from single nuclei","volume":"110","author":"Grindberg","year":"2013","unstructured":"Grindberg, R. V. et al. RNA-sequencing from single nuclei. Proceedings of the National Academy of Sciences. 110 (49), 19802-19807 (2013).","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"107","key":"ref38","first-page":"e53561","type":"journal-article","article-title":"Whole mount dissection and immunofluorescence of the adult mouse cochlea","author":"Montgomery","year":"2016","unstructured":"Montgomery, S. C., Cox, B. C. Whole mount dissection and immunofluorescence of the adult mouse cochlea. Journal of Visuazlied Experiments. (107), 53561 (2016).","journal-title":"Journal of Visuazlied Experiments"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/65254\/dissection-adult-mouse-stria-vascularis-for-single-nucleus-sequencing","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:17:56Z","timestamp":1792606676000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/65254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,21]]},"references-count":38,"journal-issue":{"issue":"194","published-online":{"date-parts":[[2023]]}},"URL":"https:\/\/doi.org\/10.3791\/65254","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,21]]}}}