{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:39:46Z","timestamp":1792607986199,"version":"4.5.0"},"reference-count":18,"publisher":"MyJove Corporation","issue":"197","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/65477","type":"journal-article","created":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T20:00:15Z","timestamp":1688760015000},"source":"Crossref","is-referenced-by-count":2,"title":["Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination"],"prefix":"10.3791","author":[{"given":"Barbara J.","family":"Stone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah J.","family":"Srodulski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2023,7,7]]},"reference":[{"key":"ref1","type":"book","volume-title":"Manipulating the Mouse Embryo: A Laboratory Manual, fourth edition","author":"Behringer","year":"2014","unstructured":"Behringer, R., Gertsenstein, M., Nagy, K. V., Nagy, A. Manipulating the Mouse Embryo: A Laboratory Manual, fourth edition. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY (2014)."},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"3604","DOI":"10.1038\/s41598-023-30774-x","type":"journal-article","article-title":"Successful induction of pseudopregnancy using sonic vibration in mice","volume":"13","author":"Wake","year":"2023","unstructured":"Wake, Y. et al. Successful induction of pseudopregnancy using sonic vibration in mice. Scientific Reports. 13 (1), 3604 (2023).","journal-title":"Scientific Reports"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1007\/s11248-015-9887-3","type":"journal-article","article-title":"A rapid and effective nonsurgical artificial insemination protocol using the NSET device for sperm transfer in mice without anesthesia","volume":"24","author":"Stone","year":"2015","unstructured":"Stone, B. J., Steele, K. H., Fath-Goodin, A. A rapid and effective nonsurgical artificial insemination protocol using the NSET device for sperm transfer in mice without anesthesia. Transgenic Research. 24 (4), 775-781 (2015).","journal-title":"Transgenic Research"},{"issue":"5","key":"ref4","doi-asserted-by":"crossref","first-page":"919","DOI":"10.2144\/000113257","type":"journal-article","article-title":"A device for the simple and rapid transcervical transfer of mouse embryos eliminates the need for surgery and potential post-operative complications","volume":"47","author":"Green","year":"2009","unstructured":"Green, M., Bass, S., Spear, B. A device for the simple and rapid transcervical transfer of mouse embryos eliminates the need for surgery and potential post-operative complications. BioTechniques. 47 (5), 919-924 (2009).","journal-title":"BioTechniques"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1007\/s11248-014-9802-3","type":"journal-article","article-title":"Improved pregnancy and birth rates with routine application of nonsurgical embryo transfer","volume":"23","author":"Bin Ali","year":"2014","unstructured":"Bin Ali, R. et al. Improved pregnancy and birth rates with routine application of nonsurgical embryo transfer. Transgenic Research. 23 (4), 691-695 (2014).","journal-title":"Transgenic Research"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.theriogenology.2020.03.007","type":"journal-article","article-title":"Media optimization to promote rat embryonic development to the blastocyst stage in vitro","volume":"151","author":"Men","year":"2020","unstructured":"Men, H., Stone, B. J., Bryda, E. C. Media optimization to promote rat embryonic development to the blastocyst stage in vitro. Theriogenology. 151, 81-85 (2020).","journal-title":"Theriogenology"},{"issue":"5","key":"ref7","doi-asserted-by":"crossref","first-page":"488","DOI":"10.30802\/AALAS-JAALAS-19-000163","type":"journal-article","article-title":"A nonsurgical embryo transfer technique for fresh and cultured blastocysts in rats","volume":"59","author":"Stone","year":"2020","unstructured":"Stone, B. J. et al. A nonsurgical embryo transfer technique for fresh and cultured blastocysts in rats. Journal of the American Association for Laboratory Animal Science. 59 (5), 488-495 (2020).","journal-title":"Journal of the American Association for Laboratory Animal Science"},{"issue":"1","key":"ref8","first-page":"17","type":"journal-article","article-title":"Nonsurgical embryo transfer device compared with surgery for embryo transfer in mice","volume":"52","author":"Steele","year":"2013","unstructured":"Steele, K. H. et al. Nonsurgical embryo transfer device compared with surgery for embryo transfer in mice. Journal of the American Association for Laboratory Animal Science. 52 (1), 17-21 (2013).","journal-title":"Journal of the American Association for Laboratory Animal Science"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/0018-506X(90)90025-S","type":"journal-article","article-title":"Evidence for "memory" of cervical stimulation for the promotion of pregnancy in rats","volume":"24","author":"Terkel","year":"1990","unstructured":"Terkel, J., Witcher, J. A., Adler, N. T. Evidence for \"memory\" of cervical stimulation for the promotion of pregnancy in rats. Hormones and Behavior. 24 (1), 40-49 (1990).","journal-title":"Hormones and Behavior"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"2729","DOI":"10.1038\/s41598-020-59611-1","type":"journal-article","article-title":"Simple induction of pseudopregnancy by artificial stimulation using a sonic vibration in rats","volume":"10","author":"Kaneko","year":"2020","unstructured":"Kaneko, T., Endo, M., Tsunoda, S., Nakagawa, Y., Abe, H. Simple induction of pseudopregnancy by artificial stimulation using a sonic vibration in rats. Scientific Reports. 10 (1), 2729 (2020).","journal-title":"Scientific Reports"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"e35538","DOI":"10.1371\/journal.pone.0035538","type":"journal-article","article-title":"Mouse estrous cycle identification tool and images","volume":"7","author":"Byers","year":"2012","unstructured":"Byers, S. L., Wiles, M. V., Dunn, S. L., Taft, R. A. Mouse estrous cycle identification tool and images. PLoS One. 7 (4), e35538 (2012).","journal-title":"PLoS One"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1177\/0192623315570339","type":"journal-article","article-title":"Vaginal cytology of the laboratory rat and mouse: Review and criteria for the staging of the estrous cycle using stained vaginal smears","volume":"43","author":"Cora","year":"2015","unstructured":"Cora, M. C., Kooistra, L., Travlos, G. Vaginal cytology of the laboratory rat and mouse: Review and criteria for the staging of the estrous cycle using stained vaginal smears. Toxicologic Pathology. 43 (6), 776-793 (2015).","journal-title":"Toxicologic Pathology"},{"issue":"4","key":"ref13","first-page":"471","type":"journal-article","article-title":"Superovulation strategies for 6 commonly used mouse strains","volume":"50","author":"Luo","year":"2011","unstructured":"Luo, C. et al. Superovulation strategies for 6 commonly used mouse strains. Journal of the American Association for Laboratory Animal Science. 50 (4), 471-478 (2011).","journal-title":"Journal of the American Association for Laboratory Animal Science"},{"issue":"4512","key":"ref14","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1038\/177754a0","type":"journal-article","article-title":"Viability and developmental capacity of eggs from immature mice treated with gonadotrophins","volume":"177","author":"Gates","year":"1956","unstructured":"Gates, A. H. Viability and developmental capacity of eggs from immature mice treated with gonadotrophins. Nature., 177(4512), 754-755 (1956).","journal-title":"Nature"},{"key":"ref15","type":"book","volume-title":"The Principles of Humane Experimental Technique","author":"Russell","year":"1959","unstructured":"Russell W. M. S., Burch R. L. The Principles of Humane Experimental Technique. Methuen & Co., Ltd. London, UK (1959)."},{"issue":"6","key":"ref16","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1262\/jrd.2017-068","type":"journal-article","article-title":"Efficient and scheduled production of pseudopregnant female mice for embryo transfer by estrous cycle synchronization","volume":"63","author":"Hasegawa","year":"2017","unstructured":"Hasegawa, A. et al. Efficient and scheduled production of pseudopregnant female mice for embryo transfer by estrous cycle synchronization. Journal of Reproduction and Development. 63 (6), 539-545 (2017).","journal-title":"Journal of Reproduction and Development"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1530\/jrf.0.0450437","type":"journal-article","article-title":"Embryonic mortality during the pre- and post-implantation periods of pregnancy in mature mice after superovulation","volume":"45","author":"Beaumont","year":"1975","unstructured":"Beaumont, H. M., Smith, A. F. Embryonic mortality during the pre- and post-implantation periods of pregnancy in mature mice after superovulation. Journal of Reproduction and Fertility. 45 (3), 437-448 (1975).","journal-title":"Journal of Reproduction and Fertility"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1093\/biolreprod\/8.4.491","type":"journal-article","article-title":"Determining the stage of the estrous cycle in the mouse by the appearance of the vagina","volume":"8","author":"Champlin","year":"1973","unstructured":"Champlin, A. K., Dorr, D. L., Gates, A. H. Determining the stage of the estrous cycle in the mouse by the appearance of the vagina. Biology of Reproduction. 8 (4), 491-494 (1973).","journal-title":"Biology of Reproduction"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/65477\/inducing-pseudopregnancy-female-mice-without-need-for-vasectomized","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T17:58:13Z","timestamp":1792605493000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/65477"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,7]]},"references-count":18,"journal-issue":{"issue":"197","published-online":{"date-parts":[[2023]]}},"URL":"https:\/\/doi.org\/10.3791\/65477","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,7]]}}}