{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T17:43:33Z","timestamp":1792604613355,"version":"4.5.0"},"reference-count":39,"publisher":"MyJove Corporation","issue":"203","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/66453","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T19:00:20Z","timestamp":1705086020000},"source":"Crossref","is-referenced-by-count":0,"title":["The Efficacy and Underlying Pathway Mechanisms of ShiDuGao Treatment for Anus Eczema Based on GEO Datasets and Network Pharmacology"],"prefix":"10.3791","author":[{"given":"Song","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanyi","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anqi","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyan","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2024,1,12]]},"reference":[{"issue":"16","key":"ref1","doi-asserted-by":"crossref","first-page":"e25623","DOI":"10.1097\/MD.0000000000025623","type":"journal-article","article-title":"Differences in microbiota between acute and chronic perianal eczema","volume":"100","author":"Ma","year":"2021","unstructured":"Ma, M., Lu, H., Yang, Z., Chen, L., Li, Y., Zhang, X. Differences in microbiota between acute and chronic perianal eczema. Medicine. 100 (16), e25623 (2021).","journal-title":"Medicine"},{"issue":"9","key":"ref2","first-page":"509","type":"journal-article","article-title":"Anal eczema","volume":"78","author":"Dietrich","year":"2021","unstructured":"Dietrich, C. F., Hoch, F. Anal eczema. Revue Therapeutique. 78 (9), 509-512 (2021).","journal-title":"Revue Therapeutique"},{"issue":"6","key":"ref3","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1007\/s00105-015-3618-9","type":"journal-article","article-title":"Differential diagnosis of anal eczema","volume":"66","author":"Dietrich","year":"2015","unstructured":"Dietrich, A., Ruzicka, T., Hermans, C. Differential diagnosis of anal eczema. Hautarzt. 66 (6), 400-407 (2015).","journal-title":"Hautarzt"},{"issue":"6","key":"ref4","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1055\/s-2008-1017504","type":"journal-article","article-title":"Anal eczema, condylomata acuminata","volume":"133","author":"Rohde","year":"2008","unstructured":"Rohde, H. Anal eczema, condylomata acuminata. Deutsche Medizinische Wochenschrift (1946). 133 (6), 245-246 (2008).","journal-title":"Deutsche Medizinische Wochenschrift"},{"issue":"11","key":"ref5","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1111\/ijcp.12457","type":"journal-article","article-title":"Therapeutic management of anal eczema: an evidence-based review","volume":"68","author":"Havlickova","year":"2014","unstructured":"Havlickova, B., Weyandt, G. H. Therapeutic management of anal eczema: an evidence-based review. International Journal of Clinical Practice. 68 (11), 1388-1399 (2014).","journal-title":"International Journal of Clinical Practice"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s40257-019-00471-5","type":"journal-article","article-title":"Characterization and analysis of the skin microbiota in Rosacea: A case-control study","volume":"21","author":"Rainer","year":"2020","unstructured":"Rainer, B. M. et al. Characterization and analysis of the skin microbiota in Rosacea: A case-control study. American Journal of Clinical Dermatology. 21 (1), 139-147 (2020).","journal-title":"American Journal of Clinical Dermatology"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"168","DOI":"10.3390\/jcm9010168","type":"journal-article","article-title":"Characterization and analysis of the skin microbiota in acne: Impact of systemic antibiotics","volume":"9","author":"Park","year":"2020","unstructured":"Park, S. Y., Kim, H. S., Lee, S. H., Kim, S. Characterization and analysis of the skin microbiota in acne: Impact of systemic antibiotics. Journal of Clinical Medicine. 9 (1), 168 (2020).","journal-title":"Journal of Clinical Medicine"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"185","DOI":"10.3390\/jcm9010185","type":"journal-article","article-title":"Characterization and analysis of the skin microbiota in Rosacea: Impact of systemic antibiotics","volume":"9","author":"Woo","year":"2020","unstructured":"Woo, Y. R., Lee, S. H., Cho, S. H., Lee, J. D., Kim, H. S. Characterization and analysis of the skin microbiota in Rosacea: Impact of systemic antibiotics. Journal of Clinical Medicine. 9 (1), 185 (2020).","journal-title":"Journal of Clinical Medicine"},{"issue":"9","key":"ref9","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1007\/s10096-019-03598-9","type":"journal-article","article-title":"Alterations in the skin microbiome are associated with disease severity and treatment in the perioral zone of the skin of infants with atopic dermatitis","volume":"38","author":"Zheng","year":"2019","unstructured":"Zheng, Y. et al. Alterations in the skin microbiome are associated with disease severity and treatment in the perioral zone of the skin of infants with atopic dermatitis. European Journal of Clinical Microbiology & Infectious Diseases. 38 (9), 1677-1685 (2019).","journal-title":"European Journal of Clinical Microbiology & Infectious Diseases"},{"issue":"4","key":"ref10","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1111\/bjd.17755","type":"journal-article","article-title":"Nasal and skin microbiomes are associated with disease severity in paediatric atopic dermatitis","volume":"181","author":"Tott\u00e9","year":"2019","unstructured":"Tott\u00e9, J. E. E. et al. Nasal and skin microbiomes are associated with disease severity in paediatric atopic dermatitis. The British Journal of Dermatology. 181 (4), 796-804 (2019).","journal-title":"The British Journal of Dermatology"},{"issue":"193","key":"ref11","first-page":"e64847","type":"journal-article","article-title":"Network pharmacology prediction and experimental validation of Trichosanthes-Fritillaria thunbergii action mechanism against lung adenocarcinoma","author":"Zhao","year":"2023","unstructured":"Zhao, X. Y., Yang, Y. Y., Feng, J. l., Feng, C. l. Network pharmacology prediction and experimental validation of Trichosanthes-Fritillaria thunbergii action mechanism against lung adenocarcinoma. Journal of Visualized Experiments. 193, e64847 (2023).","journal-title":"Journal of Visualized Experiments"},{"issue":"194","key":"ref12","first-page":"e65071","type":"journal-article","article-title":"Network pharmacology prediction and metabolomics validation of the mechanism of Fructus Phyllanthi against hyperlipidemia","author":"Zeng","year":"2023","unstructured":"Zeng, B. et al. Network pharmacology prediction and metabolomics validation of the mechanism of Fructus Phyllanthi against hyperlipidemia. Journal of Visualized Experiments. 194, e65071 (2023).","journal-title":"Journal of Visualized Experiments"},{"issue":"28","key":"ref13","doi-asserted-by":"crossref","first-page":"e29593","DOI":"10.1097\/MD.0000000000029593","type":"journal-article","article-title":"The mechanism of action of the combination of Astragalus membranaceus and Ligusticum chuanxiong in the treatment of ischemic stroke based on network pharmacology and molecular docking","volume":"101","author":"Wang","year":"2022","unstructured":"Wang, T., Jiang, X., Ruan, Y., Li, L., Chu, L. The mechanism of action of the combination of Astragalus membranaceus and Ligusticum chuanxiong in the treatment of ischemic stroke based on network pharmacology and molecular docking. Medicine (Baltimore). 101 (28), e29593 (2022).","journal-title":"Medicine"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"13767","DOI":"10.1038\/s41598-023-41101-9","type":"journal-article","article-title":"Exploring the mechanism of luteolin by regulating microglia polarization based on network pharmacology and in vitro experiments","volume":"13","author":"Wang","year":"2023","unstructured":"Wang, T. et al. Exploring the mechanism of luteolin by regulating microglia polarization based on network pharmacology and in vitro experiments. Scientific Reports. 13 (1), 13767 (2023).","journal-title":"Scientific Reports"},{"issue":"1","key":"ref15","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1186\/s13020-020-00406-x","type":"journal-article","article-title":"From natural dye to herbal medicine: a systematic review of chemical constituents, pharmacological effects and clinical applications of indigo naturalis","volume":"15","author":"Qi-Yue","year":"2020","unstructured":"Qi-Yue, Y. et al. From natural dye to herbal medicine: a systematic review of chemical constituents, pharmacological effects and clinical applications of indigo naturalis. Chinese Medicine. 15 (1), 127 (2020).","journal-title":"Chinese Medicine"},{"issue":"3","key":"ref16","first-page":"104","type":"journal-article","article-title":"Juniperus ashei: the gold standard of the Cuppressaceae","volume":"32","author":"Andr\u00e9","year":"2000","unstructured":"Andr\u00e9, C., Dumur, J. P., Hrabina, M., Lefebvre, E., Sicard, H. Juniperus ashei: the gold standard of the Cuppressaceae. Allergie et Immunologie. 32 (3), 104-106 (2000).","journal-title":"Allergie et Immunologie"},{"issue":"10023","key":"ref17","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1016\/S0140-6736(15)00149-X","type":"journal-article","article-title":"Atopic dermatitis","volume":"387","author":"Weidinger","year":"2016","unstructured":"Weidinger, S., Novak, N. Atopic dermatitis. Lancet. 387 (10023), 1109-1122 (2016).","journal-title":"Lancet"},{"issue":"16","key":"ref18","first-page":"5","type":"journal-article","article-title":"Network pharmacology of Shidu ointment in the treatment of EGFR-TKIs induced acneiform eruptions","volume":"29","author":"Cai","year":"2020","unstructured":"Cai, L. L., Wu, Y., He, J. Network pharmacology of Shidu ointment in the treatment of EGFR-TKIs induced acneiform eruptions. China Pharmaceuticals. 29 (16), 5 (2020).","journal-title":"China Pharmaceuticals"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"15204","DOI":"10.1038\/s41598-020-71030-w","type":"journal-article","article-title":"Mechanisms of indigo naturalis on treating ulcerative colitis explored by GEO gene chips combined with network pharmacology and molecular docking","volume":"10","author":"Gu","year":"2020","unstructured":"Gu, S. et al. Mechanisms of indigo naturalis on treating ulcerative colitis explored by GEO gene chips combined with network pharmacology and molecular docking. Scientific Reports. 10 (1), 15204 (2020).","journal-title":"Scientific Reports"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"597601","DOI":"10.3389\/fonc.2020.597601","type":"journal-article","article-title":"Oral realgar-indigo naturalis formula plus retinoic acid for acute promyelocytic leukemia","volume":"10","author":"Lou","year":"2021","unstructured":"Lou, Y., Ma, Y., Jin, J., Zhu, H. Oral realgar-indigo naturalis formula plus retinoic acid for acute promyelocytic leukemia. Frontiers in Oncology. 10, 597601 (2021).","journal-title":"Frontiers in Oncology"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"115522","DOI":"10.1016\/j.jep.2022.115522","type":"journal-article","article-title":"Psoriasis treatment using Indigo Naturalis: Progress and strategy","volume":"297","author":"Zhang","year":"2022","unstructured":"Zhang, Q. et al. Psoriasis treatment using Indigo Naturalis: Progress and strategy. Journal of Ethnopharmacology. 297, 115522 (2022).","journal-title":"Journal of Ethnopharmacology"},{"issue":"4","key":"ref22","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1053\/j.gastro.2017.11.024","type":"journal-article","article-title":"Efficacy of Indigo Naturalis in a multicenter randomized controlled trial of patients with ulcerative colitis","volume":"154","author":"Naganuma","year":"2018","unstructured":"Naganuma, M. et al. Efficacy of Indigo Naturalis in a multicenter randomized controlled trial of patients with ulcerative colitis. Gastroenterology. 154 (4), 935-947 (2018).","journal-title":"Gastroenterology"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"674416","DOI":"10.3389\/fphar.2021.674416","type":"journal-article","article-title":"Exploring the mechanism of Indigo Naturalis in the treatment of ulcerative colitis based on TLR4\/MyD88\/NF-\u03baB signaling pathway and gut microbiota","volume":"12","author":"Yang","year":"2021","unstructured":"Yang, Q. Y. et al. Exploring the mechanism of Indigo Naturalis in the treatment of ulcerative colitis based on TLR4\/MyD88\/NF-\u03baB signaling pathway and gut microbiota. Frontiers in Pharmacology. 12, 674416 (2021).","journal-title":"Frontiers in Pharmacology"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"731","DOI":"10.3389\/fmicb.2020.00731","type":"journal-article","article-title":"Indigo Naturalis alleviates dextran sulfate sodium-induced colitis in rats via altering gut microbiota","volume":"11","author":"Sun","year":"2020","unstructured":"Sun, Z. et al. Indigo Naturalis alleviates dextran sulfate sodium-induced colitis in rats via altering gut microbiota. Frontiers in Microbiology. 11, 731 (2020).","journal-title":"Frontiers in Microbiology"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"445","DOI":"10.3389\/fphar.2017.00445","type":"journal-article","article-title":"Immune and metabolic regulation mechanism of Dangguiliuhuang decoction against insulin resistance and hepatic steatosis","volume":"8","author":"Cao","year":"2017","unstructured":"Cao, H. et al. Immune and metabolic regulation mechanism of Dangguiliuhuang decoction against insulin resistance and hepatic steatosis. Frontiers in Pharmacology. 8, 445 (2017).","journal-title":"Frontiers in Pharmacology"},{"issue":"16","key":"ref26","doi-asserted-by":"crossref","first-page":"8431","DOI":"10.3390\/ijms22168431","type":"journal-article","article-title":"Anti-inflammatory and anti-allergic effects of saponarin and its impact on signaling pathways of RAW 264.7, RBL-2H3, and HaCaT cells","volume":"22","author":"Min","year":"2021","unstructured":"Min, S. Y., Park, C. H., Yu, H. W., Park, Y. J. Anti-inflammatory and anti-allergic effects of saponarin and its impact on signaling pathways of RAW 264.7, RBL-2H3, and HaCaT cells. International Journal of Molecular Sciences. 22 (16), 8431 (2021).","journal-title":"International Journal of Molecular Sciences"},{"issue":"11","key":"ref27","doi-asserted-by":"crossref","first-page":"3005","DOI":"10.1039\/C4FO00612G","type":"journal-article","article-title":"Saponarin from barley sprouts inhibits NF-\u03baB and MAPK on LPS-induced RAW 264.7 cells","volume":"5","author":"Seo","year":"2014","unstructured":"Seo, K. H. et al. Saponarin from barley sprouts inhibits NF-\u03baB and MAPK on LPS-induced RAW 264.7 cells. Food & Function. 5 (11), 3005-3013 (2014).","journal-title":"Food & Function"},{"issue":"1","key":"ref28","doi-asserted-by":"crossref","first-page":"118","DOI":"10.3390\/molecules22010118","type":"journal-article","article-title":"A cytotoxic and anti-inflammatory campesterol derivative from genetically transformed hairy roots of Lopezia racemosa Cav. (Onagraceae)","volume":"22","author":"Moreno-Anz\u00farez","year":"2017","unstructured":"Moreno-Anz\u00farez, N. E. et al. A cytotoxic and anti-inflammatory campesterol derivative from genetically transformed hairy roots of Lopezia racemosa Cav. (Onagraceae). Molecules (Basel, Switzerland). 22 (1), 118 (2017).","journal-title":"Molecules"},{"issue":"5","key":"ref29","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1007\/s12026-022-09301-z","type":"journal-article","article-title":"Tryptanthrin attenuates TLR3-mediated STAT1 activation in THP-1 cells","volume":"70","author":"Numao","year":"2022","unstructured":"Numao, N. et al. Tryptanthrin attenuates TLR3-mediated STAT1 activation in THP-1 cells. Immunologic Research. 70 (5), 688-697 (2022).","journal-title":"Immunologic Research"},{"issue":"5","key":"ref30","doi-asserted-by":"crossref","first-page":"327","DOI":"10.2174\/1573409918666220818120645","type":"journal-article","article-title":"Melianone inhibits secreted aspartic proteases (SAP), a virulence factor during hyphal formation in Candida albicans","volume":"18","author":"Veni","year":"2022","unstructured":"Veni, A., Lokeswari, T. S., Pavithra, D., Sugapriya, T. Melianone inhibits secreted aspartic proteases (SAP), a virulence factor during hyphal formation in Candida albicans. Current Computer-Aided Drug Design. 18 (5), 327-336 (2022).","journal-title":"Current Computer-Aided Drug Design"},{"issue":"10","key":"ref31","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1007\/s13205-020-02441-9","type":"journal-article","article-title":"Bioactivity of melianone against Salmonella and in silico prediction of a membrane protein target","volume":"10","author":"Veni","year":"2020","unstructured":"Veni, A., Lokeswari, T. S., Krishna Kumari, G. N., Gayathri, D., Sudandiradoss, C. Bioactivity of melianone against Salmonella and in silico prediction of a membrane protein target. 3 Biotech. 10 (10), 460 (2020).","journal-title":"3 Biotech"},{"issue":"16","key":"ref32","doi-asserted-by":"crossref","first-page":"e25623","DOI":"10.1097\/MD.0000000000025623","type":"journal-article","article-title":"Differences in microbiota between acute and chronic perianal eczema","volume":"100","author":"Ma","year":"2021","unstructured":"Ma, M., Lu, H., Yang, Z., Chen, L., Li, Y., Zhang, X. Differences in microbiota between acute and chronic perianal eczema. Medicine. 100 (16), e25623 (2021).","journal-title":"Medicine"},{"issue":"12","key":"ref33","doi-asserted-by":"crossref","DOI":"10.2340\/00015555-3516","type":"journal-article","article-title":"Prevention of atopic dermatitis","volume":"100","author":"Williams","year":"2020","unstructured":"Williams, H. C., Chalmers, J. Prevention of atopic dermatitis. Acta Dermato-Venereologica. 100 (12), adv00166 (2020).","journal-title":"Acta Dermato-Venereologica"},{"issue":"2","key":"ref34","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.tips.2021.11.004","type":"journal-article","article-title":"Network pharmacology: curing causal mechanisms instead of treating symptoms","volume":"43","author":"Nogales","year":"2022","unstructured":"Nogales, C., Mamdouh, Z. M., List, M., Kiel, C., Casas, A. I., Schmidt, H. H. H. W. Network pharmacology: curing causal mechanisms instead of treating symptoms. Trends in Pharmacological Sciences. 43 (2), 136-150 (2022).","journal-title":"Trends in Pharmacological Sciences"},{"issue":"26","key":"ref35","doi-asserted-by":"crossref","first-page":"e29762","DOI":"10.1097\/MD.0000000000029762","type":"journal-article","article-title":"Prediction and validation of potential molecular targets for the combination of Astragalus membranaceus and Angelica sinensis in the treatment of atherosclerosis based on network pharmacology","volume":"101","author":"Wang","year":"2022","unstructured":"Wang, T., Zhou, Y., Wang, K., Jiang, X., Wang, J., Chen, J. Prediction and validation of potential molecular targets for the combination of Astragalus membranaceus and Angelica sinensis in the treatment of atherosclerosis based on network pharmacology. Medicine (Baltimore). 101 (26), e29762 (2022).","journal-title":"Medicine (Baltimore)"},{"issue":"3","key":"ref36","doi-asserted-by":"crossref","first-page":"e32693","DOI":"10.1097\/MD.0000000000032693","type":"journal-article","article-title":"Exploration of Fuzheng Yugan mixture on COVID-19 based on network pharmacology and molecular docking","volume":"102","author":"Jiang","year":"2023","unstructured":"Jiang, X. et al. Exploration of Fuzheng Yugan mixture on COVID-19 based on network pharmacology and molecular docking. Medicine (Baltimore). 102 (3), e32693 (2023).","journal-title":"Medicine (Baltimore)"},{"issue":"1","key":"ref37","doi-asserted-by":"crossref","first-page":"19496","DOI":"10.1038\/s41598-021-98925-6","type":"journal-article","article-title":"Network pharmacology-based investigation of potential targets of astragalus membranaceous-angelica sinensis compound acting on diabetic nephropathy","volume":"11","author":"Dong","year":"2021","unstructured":"Dong, Y., Zhao, Q., Wang, Y. Network pharmacology-based investigation of potential targets of astragalus membranaceous-angelica sinensis compound acting on diabetic nephropathy. Scientific Reports. 11 (1), 19496 (2021).","journal-title":"Scientific Reports"},{"issue":"5","key":"ref38","first-page":"1543","type":"journal-article","article-title":"Identification and integration analysis of a novel prognostic signature associated with cuproptosis-related ferroptosis genes and relevant lncRNA regulatory axis in lung adenocarcinoma","volume":"15","author":"Wang","year":"2023","unstructured":"Wang, T., Jiang, X., Lu, Y., Ruan, Y., Wang, J. Identification and integration analysis of a novel prognostic signature associated with cuproptosis-related ferroptosis genes and relevant lncRNA regulatory axis in lung adenocarcinoma. Aging (Albany NY). 15 (5), 1543-1563 (2023).","journal-title":"Aging (Albany NY)"},{"issue":"5","key":"ref39","doi-asserted-by":"crossref","first-page":"13767","DOI":"10.1080\/21655979.2022.2084253","type":"journal-article","article-title":"Based on network pharmacology and in vitro experiments to prove the effective inhibition of myocardial fibrosis by Buyang Huanwu decoction)","volume":"13","author":"Wang","year":"2022","unstructured":"Wang, T., Jiang, X., Ruan, Y., Zhuang, J., Yin, Y. Based on network pharmacology and in vitro experiments to prove the effective inhibition of myocardial fibrosis by Buyang Huanwu decoction. Bioengineered. 13 (5), 13767-13783 (2022).","journal-title":"Bioengineered"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/66453\/the-efficacy-underlying-pathway-mechanisms-shidugao-treatment-for","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T17:21:26Z","timestamp":1792603286000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/66453"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,12]]},"references-count":39,"journal-issue":{"issue":"203","published-online":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.3791\/66453","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,12]]}}}