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J Virol. 72 (4), 2855 (1998).","journal-title":"J Virol"},{"issue":"9","key":"ref25","doi-asserted-by":"crossref","first-page":"4653","DOI":"10.1073\/pnas.94.9.4653","type":"journal-article","article-title":"A new reporter cell line to monitor HIV infection and drug susceptibility in vitro","volume":"94","author":"Gervaix","year":"1997","unstructured":"Gervaix, A., West, D., Leoni, L. M., Richman, D. D., Wong-Staal, F., Corbeil, J. A new reporter cell line to monitor HIV infection and drug susceptibility in vitro. 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Trends Pharmacol Sci. 19 (4), 131-135 (1998).","journal-title":"Trends Pharmacol Sci"},{"issue":"6","key":"ref29","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1016\/j.cell.2006.01.040","type":"journal-article","article-title":"A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen","volume":"124","author":"Moffat","year":"2006","unstructured":"Moffat, J. et al. A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Cell. 124 (6), 1283-1298 (2006).","journal-title":"Cell"},{"issue":"4706","key":"ref30","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1126\/science.2990028","type":"journal-article","article-title":"A Transcriptional activator protein encoded by the x-lor region of the human T-cell leukemia virus","volume":"228","author":"Sodroski","year":"1985","unstructured":"Sodroski, J., Rosen, C., Goh, W. C., Haseltine, W. 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