{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T17:39:59Z","timestamp":1792604399434,"version":"4.5.0"},"reference-count":35,"publisher":"MyJove Corporation","issue":"205","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/66645","type":"journal-article","created":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T20:00:36Z","timestamp":1710532836000},"source":"Crossref","is-referenced-by-count":3,"title":["Revealing the Ferroptotic Phenotype of Medulloblastoma"],"prefix":"10.3791","author":[{"given":"Fabien","family":"Segui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boutaina","family":"Daher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9lia","family":"Gotorbe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Pouyssegur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Picco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milica","family":"Vucetic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2024,3,15]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1016\/j.cell.2012.03.042","type":"journal-article","article-title":"Ferroptosis: An iron-dependent form of nonapoptotic cell death","volume":"149","author":"Dixon","year":"2012","unstructured":"Dixon, S. J. et al. Ferroptosis: An iron-dependent form of nonapoptotic cell death. Cell. 149, 1060-1072 (2012).","journal-title":"Cell"},{"issue":"30","key":"ref2","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.chembiol.2008.02.010","type":"journal-article","article-title":"Synthetic lethal screening identifies compounds activating iron-dependent, nonapoptotic cell death in oncogenic-ras-harboring cancer cells","volume":"15","author":"Yang","year":"2008","unstructured":"Yang, W. S., Stockwell, B. R. Synthetic lethal screening identifies compounds activating iron-dependent, nonapoptotic cell death in oncogenic-ras-harboring cancer cells. Chem Biol. 15 (30), 234-245 (2008).","journal-title":"Chem Biol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.cell.2013.12.010","type":"journal-article","article-title":"Regulation of ferroptotic cancer cell death by GPX4","volume":"156","author":"Yang","year":"2014","unstructured":"Yang, W. S. et al. Regulation of ferroptotic cancer cell death by GPX4. Cell. 156, 317-331 (2014).","journal-title":"Cell"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1146\/annurev.bi.52.070183.003431","type":"journal-article","article-title":"Glutathione","volume":"52","author":"Meister","year":"1983","unstructured":"Meister, A., Anderson, M. E. Glutathione. Annu Rev Biochem. 52, 711-760 (1983).","journal-title":"Annu Rev Biochem"},{"issue":"5","key":"ref5","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1089\/ars.2011.4391","type":"journal-article","article-title":"The cystine\/glutamate antiporter system xc- in health and disease: From molecular mechanisms to novel therapeutic opportunities","volume":"18","author":"Lewerenzm","year":"2013","unstructured":"Lewerenzm, J. et al. The cystine\/glutamate antiporter system xc- in health and disease: From molecular mechanisms to novel therapeutic opportunities. Antioxid Redox Signal. 18 (5), 522-555 (2013).","journal-title":"Antioxid Redox Signal"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.cell.2013.12.010","type":"journal-article","article-title":"Regulation of ferroptotic cancer cell death by GPX4","volume":"156","author":"Yang","year":"2014","unstructured":"Yang, W. S. et al. Regulation of ferroptotic cancer cell death by GPX4. Cell. 156, 317-331 (2014).","journal-title":"Cell"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/s41586-019-1705-2","type":"journal-article","article-title":"The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis","volume":"575","author":"Bersuker","year":"2019","unstructured":"Bersuker, K. et al. The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis. Nature. 575, 688-692 (2019).","journal-title":"Nature"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1038\/s41586-019-1707-0","type":"journal-article","article-title":"FSP1 is a glutathione-independent ferroptosis suppressor","volume":"575","author":"Doll","year":"2019","unstructured":"Doll, S. et al. FSP1 is a glutathione-independent ferroptosis suppressor. Nature. 575, 693-698 (2019).","journal-title":"Nature"},{"key":"ref9","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2022.810327","type":"journal-article","article-title":"Blockade of GCH1\/BH4 axis activates ferritinophagy to mitigate the resistance of colorectal cancer to erastin-induced ferroptosis","volume":"10","author":"Hu","year":"2022","unstructured":"Hu, Q. et al. Blockade of GCH1\/BH4 axis activates ferritinophagy to mitigate the resistance of colorectal cancer to erastin-induced ferroptosis. Front Cell Dev Biol. 10, doi.org\/10.3389\/fcell.2022.810327 (2022).","journal-title":"Front Cell Dev Biol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1038\/s41568-022-00459-0","type":"journal-article","article-title":"Targeting ferroptosis as a vulnerability in cancer","volume":"22","author":"Lei","year":"2022","unstructured":"Lei, G., Zhuang, L., Gan, B. Targeting ferroptosis as a vulnerability in cancer. Nat Rev Cancer. 22, 381-396 (2022).","journal-title":"Nat Rev Cancer"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1038\/nature23007","type":"journal-article","article-title":"Dependency of a therapy-resistant state of cancer cells on a lipid peroxidase pathway","volume":"547","author":"Viswanathan","year":"2017","unstructured":"Viswanathan, V. S. et al. Dependency of a therapy-resistant state of cancer cells on a lipid peroxidase pathway. Nature. 547, 453-457 (2017).","journal-title":"Nature"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"101697","DOI":"10.1016\/j.redox.2020.101697","type":"journal-article","article-title":"Epigenetic reprogramming of epithelial-mesenchymal transition promotes ferroptosis of head and neck cancer","volume":"37","author":"Lee","year":"2020","unstructured":"Lee, J., You, J. H., Kim, M. S., Roh, J. L. Epigenetic reprogramming of epithelial-mesenchymal transition promotes ferroptosis of head and neck cancer. Redox Biol. 37, 101697 (2020).","journal-title":"Redox Biol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1038\/nature24297","type":"journal-article","article-title":"Drug-tolerant persister cancer cells are vulnerable to GPX4 inhibition","volume":"551","author":"Hangauer","year":"2017","unstructured":"Hangauer, M. J. et al. Drug-tolerant persister cancer cells are vulnerable to GPX4 inhibition. Nature. 551, 247-250 (2017).","journal-title":"Nature"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/s41392-022-00917-z","type":"journal-article","article-title":"Thrombin induces ACSL4-dependent ferroptosis during cerebral ischemia\/reperfusion","volume":"7","author":"Zhang Tuo","year":"2022","unstructured":"Zhang Tuo, Q. et al. Thrombin induces ACSL4-dependent ferroptosis during cerebral ischemia\/reperfusion. Signal Transduct Target Ther. 7, 59 (2022).","journal-title":"Signal Transduct Target Ther"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.3389\/fnagi.2022.904152","type":"journal-article","article-title":"Mechanisms of ferroptosis and emerging links to the pathology of neurodegenerative diseases","volume":"14","author":"Sun","year":"2022","unstructured":"Sun, Y. Mechanisms of ferroptosis and emerging links to the pathology of neurodegenerative diseases. Front Aging Neurosci. 14, doi.org\/10.3389\/fnagi.2022.904152 (2022).","journal-title":"Front Aging Neurosci"},{"key":"ref16","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2022.974156","type":"journal-article","article-title":"Emerging role of ferroptosis in glioblastoma: Therapeutic opportunities and challenges","volume":"9","author":"Zhuo","year":"2022","unstructured":"Zhuo, S. Emerging role of ferroptosis in glioblastoma: Therapeutic opportunities and challenges. Front Mol Biosci. 9, doi.org\/10.3389\/fmolb.2022.974156 (2022).","journal-title":"Front Mol Biosci"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s40591-015-0045-3","type":"journal-article","article-title":"Proteomic analysis of extracellular vesicles from medullospheres reveals a role for iron in the cancer progression of medulloblastoma","volume":"3","author":"Bisaro","year":"2015","unstructured":"Bisaro, B. Proteomic analysis of extracellular vesicles from medullospheres reveals a role for iron in the cancer progression of medulloblastoma. Mol Cell Ther. 3, 8 (2015).","journal-title":"Mol Cell Ther"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.ccell.2015.09.002","type":"journal-article","article-title":"Preferential iron trafficking characterizes glioblastoma stem-like cells","volume":"28","author":"Schonberg","year":"2015","unstructured":"Schonberg, D. L. et al. Preferential iron trafficking characterizes glioblastoma stem-like cells. Cancer Cell. 28 (4), 441-455 (2015).","journal-title":"Cancer Cell"},{"issue":"7","key":"ref19","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1111\/febs.15817","type":"journal-article","article-title":"From sorting to sequencing in the molecular era: the evolution of the cancer stem cell model in medulloblastoma","volume":"289","author":"Werbowetski-Ogilvie","year":"2022","unstructured":"Werbowetski-Ogilvie, T. E. From sorting to sequencing in the molecular era: the evolution of the cancer stem cell model in medulloblastoma. FEBS J. 289 (7), 1765-1778 (2022).","journal-title":"FEBS J"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"e02523","DOI":"10.7554\/eLife.02523","type":"journal-article","article-title":"Pharmacological inhibition of cystine-glutamate exchange induces endoplasmic reticulum stress and ferroptosis","volume":"3","author":"Dixon","year":"2014","unstructured":"Dixon, S. J. Pharmacological inhibition of cystine-glutamate exchange induces endoplasmic reticulum stress and ferroptosis. Elife. 3, e02523 (2014).","journal-title":"Elife"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e592","DOI":"10.1038\/cddis.2013.87","type":"journal-article","article-title":"Iron modulates cell survival in a Ras- and MAPK-dependent manner in ovarian cells","volume":"4","author":"Bauckman","year":"2013","unstructured":"Bauckman, K. A., Haller, E., Flores, I., Nanjundan, M. Iron modulates cell survival in a Ras- and MAPK-dependent manner in ovarian cells. Cell Death Dis. 4, e592 (2013).","journal-title":"Cell Death Dis"},{"issue":"4","key":"ref22","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/S0891-5849(02)00848-1","type":"journal-article","article-title":"C11-BODIPY581\/591, an oxidation-sensitive fluorescent lipid peroxidation probe: (Micro)spectroscopic characterization and validation of methodology","volume":"33","author":"Drummen","year":"2002","unstructured":"Drummen, G. P. C., Van Liebergen, L. C. M., Op den Kamp, J. A. F., Post, J. A. C11-BODIPY581\/591, an oxidation-sensitive fluorescent lipid peroxidation probe: (Micro)spectroscopic characterization and validation of methodology. Free Radic Biol Med. 33 (4), 473-490 (2002).","journal-title":"Free Radic Biol Med"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"101328","DOI":"10.1016\/j.redox.2019.101328","type":"journal-article","article-title":"Insight into the mechanism of ferroptosis inhibition by ferrostatin-1","volume":"28","author":"Miotto","year":"2020","unstructured":"Miotto, G. Insight into the mechanism of ferroptosis inhibition by ferrostatin-1. Redox Biol. 28, 101328 (2020).","journal-title":"Redox Biol"},{"issue":"15","key":"ref24","doi-asserted-by":"crossref","first-page":"3877","DOI":"10.1158\/0008-5472.CAN-18-3855","type":"journal-article","article-title":"Genetic ablation of the cystine transporter xCT in PDAC cells inhibits mTORC1, growth, survival, and tumor formation via nutrient and oxidative stresses","volume":"79","author":"Daher","year":"2019","unstructured":"Daher, B. Genetic ablation of the cystine transporter xCT in PDAC cells inhibits mTORC1, growth, survival, and tumor formation via nutrient and oxidative stresses. Cancer Res. 79 (15), 3877-3890 (2019).","journal-title":"Cancer Res"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1038\/nchembio.2079","type":"journal-article","article-title":"Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis","volume":"12","author":"Shimada","year":"2016","unstructured":"Shimada, K. Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis. Nat Chem Biol. 12, 497-503 (2016).","journal-title":"Nat Chem Biol"},{"issue":"34","key":"ref26","doi-asserted-by":"crossref","first-page":"E4966","DOI":"10.1073\/pnas.1603244113","type":"journal-article","article-title":"Peroxidation of polyunsaturated fatty acids by lipoxygenases drives ferroptosis","volume":"113","author":"Yang","year":"2016","unstructured":"Yang, W. S. Peroxidation of polyunsaturated fatty acids by lipoxygenases drives ferroptosis. Proc Natl Acad Sci U S A. 113 (34), E4966-4975 (2016).","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1038\/s41589-018-0031-6","type":"journal-article","article-title":"FINO2 initiates ferroptosis through GPX4 inactivation and iron oxidation","volume":"14","author":"Gaschler","year":"2018","unstructured":"Gaschler, M. M. FINO2 initiates ferroptosis through GPX4 inactivation and iron oxidation. Nat Chem Biol. 14, 507-515 (2018).","journal-title":"Nat Chem Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"e37503","DOI":"10.7554\/eLife.37503","type":"journal-article","article-title":"Tryparedoxin peroxidase-deficiency commits trypanosomes to ferroptosis-type cell death","volume":"7","author":"Bogacz","year":"2018","unstructured":"Bogacz, M., Krauth-Siegel, R. L. Tryparedoxin peroxidase-deficiency commits trypanosomes to ferroptosis-type cell death. Elife. 7, e37503 (2018).","journal-title":"Elife"},{"issue":"10","key":"ref29","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1002\/cyto.a.22338","type":"journal-article","article-title":"Optimization of the C11-BODIPY581\/591 dye for the determination of lipid oxidation in Chlamydomonas reinhardtii by flow cytometry","volume":"83","author":"Cheloni","year":"2013","unstructured":"Cheloni, G., Slaveykova, V. I. Optimization of the C11-BODIPY581\/591 dye for the determination of lipid oxidation in Chlamydomonas reinhardtii by flow cytometry. Cytom Part A. 83 (10), 952-961 (2013).","journal-title":"Cytom Part A"},{"issue":"7","key":"ref30","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1016\/j.bmcl.2007.01.080","type":"journal-article","article-title":"Advantages and limitation of BODIPY as a probe for the evaluation of lipid peroxidation and its inhibition by antioxidants in plasma","volume":"17","author":"Itoh","year":"2007","unstructured":"Itoh, N., Cao, J., Chen, Z. H., Yoshida, Y., Niki, E. Advantages and limitation of BODIPY as a probe for the evaluation of lipid peroxidation and its inhibition by antioxidants in plasma. Bioorganic Med Chem Lett. 17 (7), 2059-2063 (2007).","journal-title":"Bioorganic Med Chem Lett"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1038\/s41598-018-19213-4","type":"journal-article","article-title":"The ferroptosis inducer erastin irreversibly inhibits system xc\u2212 and synergizes with cisplatin to increase cisplatin's cytotoxicity in cancer cells","volume":"8","author":"Sato","year":"2018","unstructured":"Sato, M. et al. The ferroptosis inducer erastin irreversibly inhibits system xc\u2212 and synergizes with cisplatin to increase cisplatin's cytotoxicity in cancer cells. Sci Rep. 8, 968 (2018).","journal-title":"Sci Rep"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"102703","DOI":"10.1016\/j.redox.2023.102703","type":"journal-article","article-title":"The ferroptosis inducing compounds RSL3 and ML162 are not direct inhibitors of GPX4 but of TXNRD1","volume":"62","author":"Cheff","year":"2023","unstructured":"Cheff, D. M. et al. The ferroptosis inducing compounds RSL3 and ML162 are not direct inhibitors of GPX4 but of TXNRD1. Redox Biol. 62, 102703 (2023).","journal-title":"Redox Biol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"115072","DOI":"10.1016\/j.ejmech.2022.115072","type":"journal-article","article-title":"Dual degradation mechanism of GPX4 degrader in induction of ferroptosis exerting anti-resistant tumor effect","volume":"247","author":"Wang","year":"2023","unstructured":"Wang, C. et al. Dual degradation mechanism of GPX4 degrader in induction of ferroptosis exerting anti-resistant tumor effect. Eur J Med Chem. 247, 115072 (2023).","journal-title":"Eur J Med Chem"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1038\/s41419-020-02994-w","type":"journal-article","article-title":"Together we stand, apart we fall: how cell-to-cell contact\/interplay provides resistance to ferroptosis","volume":"11","author":"Vucetic","year":"2020","unstructured":"Vucetic, M. et al. Together we stand, apart we fall: how cell-to-cell contact\/interplay provides resistance to ferroptosis. Cell Death Dis. 11, 789 (2020).","journal-title":"Cell Death Dis"},{"issue":"6","key":"ref35","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.3390\/cancers13061434","type":"journal-article","article-title":"A cystine-cysteine intercellular shuttle prevents ferroptosis in xctko pancreatic ductal adenocarcinoma cells","volume":"13","author":"Meira","year":"2021","unstructured":"Meira, W. et al. A cystine-cysteine intercellular shuttle prevents ferroptosis in xctko pancreatic ductal adenocarcinoma cells. Cancers (Basel). 13 (6), 1434 (2021).","journal-title":"Cancers (Basel)"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/66645\/revealing-the-ferroptotic-phenotype-of-medulloblastoma","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T17:08:05Z","timestamp":1792602485000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/66645"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,15]]},"references-count":35,"journal-issue":{"issue":"205","published-online":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.3791\/66645","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,15]]}}}