{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:44:41Z","timestamp":1792601081863,"version":"4.5.0"},"reference-count":34,"publisher":"MyJove Corporation","issue":"207","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/67060","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T20:02:47Z","timestamp":1717185767000},"source":"Crossref","is-referenced-by-count":2,"title":["A Modified Model Preparation for Middle Cerebral Artery Occlusion Reperfusion"],"prefix":"10.3791","author":[{"given":"Rong","family":"Ma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianlong","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danni","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuxin","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialiang","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2024,5,31]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"113518","DOI":"10.1016\/j.expneurol.2020.113518","type":"journal-article","article-title":"Emerging neuroprotective strategies for the treatment of ischemic stroke: An overview of clinical and preclinical studies","volume":"335","author":"Paul","year":"2021","unstructured":"Paul, S., Candelario-Jalil, E. Emerging neuroprotective strategies for the treatment of ischemic stroke: An overview of clinical and preclinical studies. Exp Neurol. 335, 113518 (2021).","journal-title":"Exp Neurol"},{"issue":"12","key":"ref2","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1016\/S1474-4422(23)00277-6","type":"journal-article","article-title":"Pragmatic solutions to reduce the global burden of stroke: a World Stroke Organization-Lancet Neurology Commission","volume":"22","author":"Feigin","year":"2023","unstructured":"Feigin, V. L, Owolabi, M. O. Pragmatic solutions to reduce the global burden of stroke: a World Stroke Organization-Lancet Neurology Commission. Lancet Neurol. 22 (12), 1160-1206 (2023).","journal-title":"Lancet Neurol"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1212\/CON.0000000000000833","type":"journal-article","article-title":"Ischemic Stroke in Young Adults","volume":"26","author":"Putaala","year":"2020","unstructured":"Putaala, J. Ischemic Stroke in Young Adults. Continuum (Minneapolis, Minn.). 26 (2), 386-414 (2020).","journal-title":"Continuum (Minneapolis, Minn)"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"116643","DOI":"10.1016\/j.jns.2019.116643","type":"journal-article","article-title":"A contemporary and comprehensive analysis of the costs of stroke in the United States","volume":"410","author":"Girotra","year":"2020","unstructured":"Girotra, T., Lekoubou, A., Bishu, K. G., Ovbiagele, B. A contemporary and comprehensive analysis of the costs of stroke in the United States. J Neurol Sci. 410, 116643 (2020).","journal-title":"J Neurol Sci"},{"issue":"8","key":"ref5","first-page":"1412","type":"journal-article","article-title":"Different strokes for different folks: The rich diversity of animal models of focal cerebral ischemia","volume":"30","author":"Howells","year":"2010","unstructured":"Howells, D. W. et al. Different strokes for different folks: The rich diversity of animal models of focal cerebral ischemia. JCBFM. 30 (8), 1412-1431 (2010).","journal-title":"JCBFM"},{"issue":"6","key":"ref6","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1007\/s12975-022-01082-9","type":"journal-article","article-title":"Translating animal models of ischemic stroke to the human condition","volume":"14","author":"Matur","year":"2023","unstructured":"Matur, A. V. et al. Translating animal models of ischemic stroke to the human condition. Transl Stroke Res. 14 (6), 842-853 (2023).","journal-title":"Transl Stroke Res"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1002\/ana.20741","type":"journal-article","article-title":"1,026 experimental treatments in acute stroke","volume":"59","author":"O'Collins","year":"2006","unstructured":"O'Collins, V. E. et al. 1,026 experimental treatments in acute stroke. Ann Neurol. 59 (3), 467-477 (2006).","journal-title":"Ann Neurol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3995\/jstroke.8.1","type":"journal-article","article-title":"Experimental studies of ischemic brain edema 1. A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area","author":"Koizumi","year":"1986","unstructured":"Koizumi, J. Yoshida, Y. Nakazawa, T. Ooneda, G. Experimental studies of ischemic brain edema 1. A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area. Nosotchu. 1-7 (1986).","journal-title":"Nosotchu"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1161\/01.STR.20.1.84","type":"journal-article","article-title":"Reversible middle cerebral artery occlusion without craniectomy in rats","volume":"20","author":"Longa","year":"1989","unstructured":"Longa, E. Z., Weinstein, P. R., Carlson, S., Cummins, R. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 20 (1), 84-91 (1989).","journal-title":"Stroke"},{"issue":"3","key":"ref10","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s12975-016-0508-0","type":"journal-article","article-title":"Sex differences in the cerebral collateral circulation","volume":"8","author":"Faber","year":"2017","unstructured":"Faber, J. E., Moore, S. M., Lucitti, J. L., Aghajanian, A., Zhang, H. Sex differences in the cerebral collateral circulation. Transl Stroke Res. 8(3), 273-283 (2017).","journal-title":"Transl Stroke Res"},{"issue":"11","key":"ref11","doi-asserted-by":"crossref","first-page":"2080","DOI":"10.1177\/0271678X221109873","type":"journal-article","article-title":"Redefining the Koizumi model of mouse cerebral ischemia: A comparative longitudinal study of cerebral and retinal ischemia in the Koizumi and Longa middle cerebral artery occlusion models","volume":"42","author":"Justi\u0107","year":"2022","unstructured":"Justi\u0107, H. et al. Redefining the Koizumi model of mouse cerebral ischemia: A comparative longitudinal study of cerebral and retinal ischemia in the Koizumi and Longa middle cerebral artery occlusion models. J Cereb Blood Flow Metab. 42 (11), 2080-2094 (2022).","journal-title":"J Cereb Blood Flow Metab"},{"issue":"11","key":"ref12","first-page":"1683","type":"journal-article","article-title":"Comparison of cerebral microcirculation perfusion in rat models of middle cerebral artery occlusion prepared through common carotid artery insertion and external carotid artery insertion","volume":"27","author":"Li","year":"2023","unstructured":"Li, Y. et al. Comparison of cerebral microcirculation perfusion in rat models of middle cerebral artery occlusion prepared through common carotid artery insertion and external carotid artery insertion. CJTER. 27 (11), 1683-1691 (2023).","journal-title":"CJTER"},{"issue":"9","key":"ref13","doi-asserted-by":"crossref","first-page":"2252","DOI":"10.1161\/01.STR.0000083625.54851.9A","type":"journal-article","article-title":"External carotid artery territory ischemia impairs outcome in the endovascular filament model of middle cerebral artery occlusion in rats","volume":"34","author":"Dittmar","year":"2003","unstructured":"Dittmar, M., Spruss, T., Schuierer, G., Horn, M. External carotid artery territory ischemia impairs outcome in the endovascular filament model of middle cerebral artery occlusion in rats. Stroke. 34 (9), 2252-2257 (2003).","journal-title":"Stroke"},{"issue":"4","key":"ref14","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s12975-011-0102-4","type":"journal-article","article-title":"A critical re-examination of the intraluminal filament MCAO model: impact of external carotid artery transection","volume":"2","author":"Trueman","year":"2011","unstructured":"Trueman, R. C. et al. A critical re-examination of the intraluminal filament MCAO model: impact of external carotid artery transection. Transl Stroke Res. 2 (4), 651-661 (2011).","journal-title":"Transl Stroke Res"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1093\/cvr\/cvx227","type":"journal-article","article-title":"Local sympathetic denervation attenuates myocardial inflammation and improves cardiac function after myocardial infarction in mice","volume":"114","author":"Ziegler","year":"2018","unstructured":"Ziegler, K. A. et al. Local sympathetic denervation attenuates myocardial inflammation and improves cardiac function after myocardial infarction in mice. Cardiovasc Res. 114 (2), 291-299 (2018).","journal-title":"Cardiovasc Res"},{"issue":"3","key":"ref16","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1093\/cvr\/cvab084","type":"journal-article","article-title":"Remodelling of adult cardiac tissue subjected to physiological and pathological mechanical load in vitro","volume":"118","author":"Pitoulis","year":"2022","unstructured":"Pitoulis, F. G. et al. Remodelling of adult cardiac tissue subjected to physiological and pathological mechanical load in vitro. Cardiovasc Res. 118 (3), 814-827 (2022).","journal-title":"Cardiovasc Res"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"110686","DOI":"10.1016\/j.biopha.2020.110686","type":"journal-article","article-title":"Animal models of cerebral ischemia: A review","volume":"131","author":"Ma","year":"2020","unstructured":"Ma, R. et al. Animal models of cerebral ischemia: A review. Biomed Pharmacother. 131, 110686 (2020).","journal-title":"Biomed Pharmacother"},{"issue":"8","key":"ref18","doi-asserted-by":"crossref","first-page":"7090","DOI":"10.1007\/s12035-018-1136-3","type":"journal-article","article-title":"Docosanoids promote neurogenesis and angiogenesis, blood-brain barrier integrity, penumbra protection, and neurobehavioral recovery after experimental ischemic stroke","volume":"55","author":"Belayev","year":"2018","unstructured":"Belayev, L. et al. Docosanoids promote neurogenesis and angiogenesis, blood-brain barrier integrity, penumbra protection, and neurobehavioral recovery after experimental ischemic stroke. Mol Neurobiol. 55 (8), 7090-7106 (2018).","journal-title":"Mol Neurobiol"},{"issue":"4","key":"ref19","doi-asserted-by":"crossref","first-page":"52","DOI":"10.3892\/ijmm.2021.4885","type":"journal-article","article-title":"Carthamin yellow improves cerebral ischemia-reperfusion injury by attenuating inflammation and ferroptosis in rats","volume":"47","author":"Guo","year":"2021","unstructured":"Guo, H. et al. Carthamin yellow improves cerebral ischemia-reperfusion injury by attenuating inflammation and ferroptosis in rats. Int J Mol Med. 47 (4), 52 (2021).","journal-title":"Int J Mol Med"},{"issue":"5","key":"ref20","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1161\/STROKEAHA.116.013165","type":"journal-article","article-title":"Determining the number of ischemic strokes potentially eligible for endovascular thrombectomy: a population-based study","volume":"47","author":"Chia","year":"2016","unstructured":"Chia, N. H. et al. Determining the number of ischemic strokes potentially eligible for endovascular thrombectomy: a population-based study. Stroke. 47 (5), 1377-1380 (2016).","journal-title":"Stroke"},{"issue":"4","key":"ref21","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1007\/s12975-015-0444-4","type":"journal-article","article-title":"Extending the time window for endovascular and pharmacological reperfusion","volume":"7","author":"Henninger","year":"2016","unstructured":"Henninger, N., Fisher, M. Extending the time window for endovascular and pharmacological reperfusion. Transl Stroke Res. 7 (4), 284-293 (2016).","journal-title":"Transl Stroke Res"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s12013-014-0394-6","type":"journal-article","article-title":"Use of Intravenous thrombolytic therapy in acute ischemic stroke patients: evaluation of clinical outcomes","volume":"72","author":"Zhang","year":"2015","unstructured":"Zhang, P. L. et al. Use of Intravenous thrombolytic therapy in acute ischemic stroke patients: evaluation of clinical outcomes. Cell Biochem Biophys. 72 (1), 11-17 (2015).","journal-title":"Cell Biochem Biophys"},{"issue":"2","key":"ref23","doi-asserted-by":"crossref","first-page":"e0148503","DOI":"10.1371\/journal.pone.0148503","type":"journal-article","article-title":"A comparative study of variables influencing ischemic injury in the Longa and Koizumi methods of intraluminal filament middle cerebral artery occlusion in mice","volume":"11","author":"Morris","year":"2016","unstructured":"Morris, G. P. et al. A comparative study of variables influencing ischemic injury in the Longa and Koizumi methods of intraluminal filament middle cerebral artery occlusion in mice. PLOS One. 11 (2), e0148503 (2016).","journal-title":"PLOS One"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.jneumeth.2015.04.008","type":"journal-article","article-title":"Critical differences between two classical surgical approaches for middle cerebral artery occlusion-induced stroke in mice","volume":"249","author":"Smith","year":"2015","unstructured":"Smith, H. K., Russell, J. M., Granger, D. N., Gavins, F. N. Critical differences between two classical surgical approaches for middle cerebral artery occlusion-induced stroke in mice. J Neurosci Methods. 249, 99-105 (2015).","journal-title":"J Neurosci Methods"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.expneurol.2005.05.013","type":"journal-article","article-title":"The role of ECA transection in the development of masticatory lesions in the MCAO filament model","volume":"195","author":"Dittmar","year":"2005","unstructured":"Dittmar, M. S. et al. The role of ECA transection in the development of masticatory lesions in the MCAO filament model. Exp Neurol. 195 (2), 372-378 (2005).","journal-title":"Exp Neurol"},{"issue":"6","key":"ref26","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1177\/0271678X16660986","type":"journal-article","article-title":"Inadequate food and water intake determine mortality following stroke in mice","volume":"37","author":"Lourbopoulos","year":"2017","unstructured":"Lourbopoulos, A. et al. Inadequate food and water intake determine mortality following stroke in mice. J Cereb Blood Flow Metab. 37 (6), 2084-2097 (2017).","journal-title":"J Cereb Blood Flow Metab"},{"issue":"13","key":"ref27","doi-asserted-by":"crossref","first-page":"9710","DOI":"10.1167\/iovs.11-8160","type":"journal-article","article-title":"Ligation of the pterygopalatine and external carotid arteries induces ischemic damage in the murine retina","volume":"52","author":"Ogishima","year":"2011","unstructured":"Ogishima, H. et al. Ligation of the pterygopalatine and external carotid arteries induces ischemic damage in the murine retina. Invest Ophth Vis Sci. 52 (13), 9710-9720 (2011).","journal-title":"Invest Ophth Vis Sci"},{"issue":"10","key":"ref28","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1177\/0271678X17720559","type":"journal-article","article-title":"Reperfusion after ischemic stroke is associated with reduced brain edema","volume":"38","author":"Irvine","year":"2018","unstructured":"Irvine, H. J. et al. Reperfusion after ischemic stroke is associated with reduced brain edema. J Cereb Blood Flow Metab. 38 (10), 1807-1817 (2018).","journal-title":"J Cereb Blood Flow Metab"},{"issue":"3","key":"ref29","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1602\/neurorx.2.3.396","type":"journal-article","article-title":"Rodent models of focal stroke: Size, mechanism, and purpose","volume":"2","author":"Carmichael","year":"2005","unstructured":"Carmichael, S. T. Rodent models of focal stroke: Size, mechanism, and purpose. NeuroRx. 2 (3), 396-409 (2005).","journal-title":"NeuroRx"},{"issue":"12","key":"ref30","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1038\/sj.jcbfm.9600298","type":"journal-article","article-title":"Bench to bedside: The quest for quality in experimental stroke research","volume":"26","author":"Dirnagl","year":"2006","unstructured":"Dirnagl, U., Dirnagl, U. Bench to bedside: The quest for quality in experimental stroke research. J Cereb Blood Flow Metab. 26 (12), 1465-1478 (2006).","journal-title":"J Cereb Blood Flow Metab"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"21086","DOI":"10.1038\/srep21086","type":"journal-article","article-title":"Method parameters' impact on mortality and variability in mouse stroke experiments: A meta-analysis","volume":"6","author":"Ingberg","year":"2016","unstructured":"Ingberg, E., Dock, H., Theodorsson, E., Theodorsson, A., Str\u00f6m, J. O. Method parameters' impact on mortality and variability in mouse stroke experiments: A meta-analysis. Sci Rep. 6, 21086 (2016).","journal-title":"Sci Rep"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.brainres.2003.10.028","type":"journal-article","article-title":"Extension of cerebral hypoperfusion and ischaemic pathology beyond MCA territory after intraluminal filament occlusion in C57Bl\/6J mice","volume":"997","author":"McColl","year":"2004","unstructured":"McColl, B. W., Carswell, H. V., McCulloch, J., Horsburgh, K. Extension of cerebral hypoperfusion and ischaemic pathology beyond MCA territory after intraluminal filament occlusion in C57Bl\/6J mice. Brain Res. 997 (1), 15-23 (2004).","journal-title":"Brain Res"},{"issue":"5","key":"ref33","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1097\/00004647-199805000-00012","type":"journal-article","article-title":"Cerebral ischemia after bilateral carotid artery occlusion and intraluminal suture occlusion in mice: Evaluation of the patency of the posterior communicating artery","volume":"18","author":"Kitagawa","year":"1998","unstructured":"Kitagawa, K. et al. Cerebral ischemia after bilateral carotid artery occlusion and intraluminal suture occlusion in mice: Evaluation of the patency of the posterior communicating artery. J Cereb Blood Flow Metab. 18 (5), 570-579 (1998).","journal-title":"J Cereb Blood Flow Metab"},{"issue":"7","key":"ref34","first-page":"931","type":"journal-article","article-title":"An alternative surgical approach reduces variability following filament induction of experimental stroke in mice","volume":"10","author":"Trotman-Lucas","year":"2017","unstructured":"Trotman-Lucas, M., Kelly, M. E., Janus, J., Fern, R., Gibson, C. L. An alternative surgical approach reduces variability following filament induction of experimental stroke in mice. Dis Model Mech. 10 (7), 931-938 (2017).","journal-title":"Dis Model Mech"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/67060\/a-modified-model-preparation-for-middle-cerebral-artery-occlusion","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:23:39Z","timestamp":1792599819000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/67060"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,31]]},"references-count":34,"journal-issue":{"issue":"207","published-online":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.3791\/67060","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,31]]}}}