{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:42:00Z","timestamp":1792600920122,"version":"4.5.0"},"reference-count":29,"publisher":"MyJove Corporation","issue":"212","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/67205","type":"journal-article","created":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T20:00:10Z","timestamp":1728676810000},"source":"Crossref","is-referenced-by-count":2,"title":["Acute Single-Unit Multi-Electrode Recordings from the Brainstem of Head-Fixed Mice"],"prefix":"10.3791","author":[{"given":"Magdalena","family":"Pikus","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elzbieta","family":"Dulko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Beenhakker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Lunardi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2024,10,11]]},"reference":[{"issue":"6","key":"ref1","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1016\/j.neuron.2015.10.032","type":"journal-article","article-title":"Monolithically integrated \u00b5LEDs on silicon neural probes for high-resolution optogenetic studies in behaving animals","volume":"88","author":"Wu","year":"2015","unstructured":"Wu, F. et al. Monolithically integrated \u00b5LEDs on silicon neural probes for high-resolution optogenetic studies in behaving animals. Neuron. 88 (6), 1136-1148 (2015).","journal-title":"Neuron"},{"issue":"6","key":"ref2","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1038\/s41583-019-0140-6","type":"journal-article","article-title":"Novel electrode technologies for neural recordings","volume":"20","author":"Hong","year":"2019","unstructured":"Hong, G., Lieber, C. M. Novel electrode technologies for neural recordings. Nat Rev Neurosci. 20 (6), 330-345 (2019).","journal-title":"Nat Rev Neurosci"},{"issue":"7600","key":"ref3","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1038\/nature17643","type":"journal-article","article-title":"Robust neuronal dynamics in premotor cortex during motor planning","volume":"532","author":"Li","year":"2016","unstructured":"Li, N., Daie, K., Svoboda, K., Druckmann, S. Robust neuronal dynamics in premotor cortex during motor planning. Nature. 532 (7600), 459-464 (2016).","journal-title":"Nature"},{"issue":"46","key":"ref4","doi-asserted-by":"crossref","first-page":"11830","DOI":"10.1523\/JNEUROSCI.3879-08.2008","type":"journal-article","article-title":"Advanced neurotechnologies for chronic neural interfaces: New horizons and clinical opportunities","volume":"28","author":"Kipke","year":"2008","unstructured":"Kipke, D. R. et al. Advanced neurotechnologies for chronic neural interfaces: New horizons and clinical opportunities. J Neurosci. 28 (46), 11830-11838 (2008).","journal-title":"J Neurosci"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.conb.2018.01.009","type":"journal-article","article-title":"Challenges and opportunities for large-scale electrophysiology with Neuropixels probes","volume":"50","author":"Steinmetz","year":"2018","unstructured":"Steinmetz, N. A., Koch, C., Harris, K. D., Carandini, M. Challenges and opportunities for large-scale electrophysiology with Neuropixels probes. Curr Opinion Neurobiol. 50, 92-100 (2018).","journal-title":"Curr Opinion Neurobiol"},{"issue":"10","key":"ref6","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1016\/j.neuron.2022.02.012","type":"journal-article","article-title":"Neural correlates of blood flow measured by ultrasound","volume":"110","author":"Nunez-Elizalde","year":"2022","unstructured":"Nunez-Elizalde, A. O. et al. Neural correlates of blood flow measured by ultrasound. Neuron. 110 (10), 1631-1640.e4 (2022).","journal-title":"Neuron"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"016036","DOI":"10.1088\/1741-2552\/ab581a","type":"journal-article","article-title":"Open source silicon microprobes for high throughput neural recording","volume":"17","author":"Yang","year":"2020","unstructured":"Yang, L., Lee, K., Villagracia, J., Masmanidis, S. C. Open source silicon microprobes for high throughput neural recording. J Neural Eng. 17 (1), 016036 (2020).","journal-title":"J Neural Eng"},{"issue":"7679","key":"ref8","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1038\/nature24636","type":"journal-article","article-title":"Fully integrated silicon probes for high-density recording of neural activity","volume":"551","author":"Jun","year":"2017","unstructured":"Jun, J. J. et al. Fully integrated silicon probes for high-density recording of neural activity. Nature. 551 (7679), 232-236 (2017).","journal-title":"Nature"},{"issue":"3","key":"ref9","doi-asserted-by":"crossref","first-page":"100194","DOI":"10.1016\/j.xpro.2020.100194","type":"journal-article","article-title":"Chronic cranial window for imaging cortical activity in head-fixed mice","volume":"1","author":"Augustinaite","year":"2020","unstructured":"Augustinaite, S., Kuhn, B. Chronic cranial window for imaging cortical activity in head-fixed mice. STAR Protoc. 1 (3), 100194 (2020).","journal-title":"STAR Protoc"},{"issue":"4","key":"ref10","doi-asserted-by":"crossref","DOI":"10.1523\/ENEURO.0146-21.2021","type":"journal-article","article-title":"Transection of the superior sagittal sinus enables bilateral access to the rodent midline brain structures","volume":"8","author":"Dias","year":"2021","unstructured":"Dias, M. et al. Transection of the superior sagittal sinus enables bilateral access to the rodent midline brain structures. eNeuro. 8 (4), ENEURO.0146-21.2021 (2021).","journal-title":"eNeuro"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.neuron.2018.11.010","type":"journal-article","article-title":"Tac1-expressing neurons in the periaqueductal gray facilitate the itch-scratching cycle via descending regulation","volume":"101","author":"Gao","year":"2019","unstructured":"Gao, Z. R. et al. Tac1-expressing neurons in the periaqueductal gray facilitate the itch-scratching cycle via descending regulation. Neuron. 101 (1), 45-59.e9 (2019).","journal-title":"Neuron"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1097\/ALN.0000000000004893","type":"journal-article","article-title":"Anatomical substrates of rapid eye movement sleep rebound in a rodent model of post-sevoflurane sleep disruption","volume":"140","author":"Atluri","year":"2023","unstructured":"Atluri, N. et al. Anatomical substrates of rapid eye movement sleep rebound in a rodent model of post-sevoflurane sleep disruption. Anesthesiology. 140 (4), 729-741 (2023).","journal-title":"Anesthesiology"},{"issue":"7623","key":"ref13","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/nature19773","type":"journal-article","article-title":"Circuit-based interrogation of sleep control","volume":"538","author":"Weber","year":"2016","unstructured":"Weber, F., Dan, Y. Circuit-based interrogation of sleep control. Nature. 538 (7623), 51-59 (2016).","journal-title":"Nature"},{"issue":"1234567890","key":"ref14","type":"journal-article","article-title":"Bidirectional control of fear memories by cerebellar neurons projecting to the ventrolateral periaqueductal grey","volume":"11","author":"Frontera","year":"2020","unstructured":"Frontera, J. et al. Bidirectional control of fear memories by cerebellar neurons projecting to the ventrolateral periaqueductal grey. Nat Commun. 11, 1234567890 (2020).","journal-title":"Nat Commun"},{"issue":"1","key":"ref15","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1038\/s41467-017-02765-w","type":"journal-article","article-title":"Regulation of REM and Non-REM sleep by periaqueductal GABAergic neurons","volume":"9","author":"Weber","year":"2018","unstructured":"Weber, F. et al. Regulation of REM and Non-REM sleep by periaqueductal GABAergic neurons. Nat Commun. 9 (1), 354 (2018).","journal-title":"Nat Commun"},{"issue":"6","key":"ref16","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/nrn3241","type":"journal-article","article-title":"The origin of extracellular fields and currents--EEG, ECoG, LFP and spikes","volume":"13","author":"Buzs\u00e1ki","year":"2012","unstructured":"Buzs\u00e1ki, G., Anastassiou, C. A., Koch, C. The origin of extracellular fields and currents--EEG, ECoG, LFP and spikes. Nat Rev Neurosci. 13 (6), 407-420 (2012).","journal-title":"Nat Rev Neurosci"},{"key":"ref17","type":"book","volume-title":"The mouse brain in stereotaxic coordinates","author":"Franklin","year":"2008","unstructured":"Franklin, K. B .J., Paxinos, G. The mouse brain in stereotaxic coordinates. Elsevier, AP. Amsterdam. (2008)."},{"key":"ref18","volume-title":"Pythagorean theorem","year":"2024","unstructured":"Pythagorean theorem. Wikipedia. at <https:\/\/en.wikipedia.org\/w\/index.php?title=Pythagorean_theorem&oldid=1222259845> (2024)."},{"key":"ref19","volume-title":"The Anatomy of the Laboratory Mouse","year":"2024","unstructured":"The Anatomy of the Laboratory Mouse. at <https:\/\/www.informatics.jax.org\/cookbook\/chapters\/skeleton.shtml>. (2024)."},{"key":"ref20","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.ibneur.2023.07.003","type":"journal-article","article-title":"Where do you measure the Bregma for rodent stereotaxic surgery","volume":"15","author":"Cecyn","year":"2023","unstructured":"Cecyn, M. N., Abrahao, K. P. Where do you measure the Bregma for rodent stereotaxic surgery? IBRO Neurosci Rep. 15, 143-148 (2023).","journal-title":"IBRO Neurosci Rep"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1038\/s41598-018-36816-z","type":"journal-article","article-title":"Slow insertion of silicon probes improves the quality of acute neuronal recordings","volume":"9","author":"Fi\u00e1th","year":"2019","unstructured":"Fi\u00e1th, R. et al. Slow insertion of silicon probes improves the quality of acute neuronal recordings. Sci Rep. 9 (1), 111 (2019).","journal-title":"Sci Rep"},{"issue":"5","key":"ref22","doi-asserted-by":"crossref","first-page":"e3988","DOI":"10.21769\/BioProtoc.3988","type":"journal-article","article-title":"Transcardiac perfusion of the mouse for brain tissue dissection and fixation","volume":"11","author":"Wu","year":"2021","unstructured":"Wu, J. et al. Transcardiac perfusion of the mouse for brain tissue dissection and fixation. Bio-protocol. 11 (5), e3988 (2021).","journal-title":"Bio-protocol"},{"key":"ref23","type":"book","volume-title":"petersaj\/AP_histology","author":"Peters","year":"2024","unstructured":"Peters, A. petersaj\/AP_histology. at <https:\/\/github.com\/petersaj\/AP_histology> (2024)."},{"key":"ref24","type":"book","volume-title":"Solving the spike sorting problem with Kilosort","author":"Pachitariu","year":"2023","unstructured":"Pachitariu, M., Sridhar, S., Stringer, C. Solving the spike sorting problem with Kilosort. (2023)."},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"3521","DOI":"10.1038\/s41598-019-39724-y","type":"journal-article","article-title":"pyPhotometry: Open source Python based hardware and software for fiber photometry data acquisition","volume":"9","author":"Akam","year":"2019","unstructured":"Akam, T., Walton, M. E. pyPhotometry: Open source Python based hardware and software for fiber photometry data acquisition. Sci Rep. 9 (1), 3521 (2019).","journal-title":"Sci Rep"},{"issue":"2022","key":"ref26","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1146\/annurev-neuro-082321-025137","type":"journal-article","article-title":"Brainstem circuits for locomotion","volume":"45","author":"Leiras","year":"2022","unstructured":"Leiras, R., Cregg, J. M., Kiehn, O. Brainstem circuits for locomotion. Ann Rev Neurosci. 45 (2022), 63-85 (2022).","journal-title":"Ann Rev Neurosci"},{"issue":"21","key":"ref27","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1212\/WNL.0000000000006537","type":"journal-article","article-title":"Brainstem integration of arousal, sleep, cardiovascular, and respiratory control","volume":"91","author":"Benarroch","year":"2018","unstructured":"Benarroch, E. E. Brainstem integration of arousal, sleep, cardiovascular, and respiratory control. Neurology. 91 (21), 958-966 (2018).","journal-title":"Neurology"},{"issue":"5","key":"ref28","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1016\/j.neuron.2015.08.001","type":"journal-article","article-title":"Neural control of breathing and CO2 homeostasis","volume":"87","author":"Guyenet","year":"2015","unstructured":"Guyenet, P. G., Bayliss, D. A. Neural control of breathing and CO2 homeostasis. Neuron. 87 (5), 946-961 (2015).","journal-title":"Neuron"},{"issue":"4","key":"ref29","doi-asserted-by":"crossref","first-page":"046020","DOI":"10.1088\/1741-2560\/9\/4\/046020","type":"journal-article","article-title":"Stab injury and device implantation within the brain results in inversely multiphasic neuroinflammatory and neurodegenerative responses","volume":"9","author":"Potter","year":"2012","unstructured":"Potter, K. A., Buck, A. C., Self, W. K., Capadona, J. R. Stab injury and device implantation within the brain results in inversely multiphasic neuroinflammatory and neurodegenerative responses. J Neural Eng. 9 (4), 046020 (2012).","journal-title":"J Neural Eng"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/67205\/acute-single-unit-multi-electrode-recordings-from-brainstem-head","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:00:29Z","timestamp":1792598429000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/67205\/acute-single-unit-multi-electrode-recordings-from-brainstem-head"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,11]]},"references-count":29,"journal-issue":{"issue":"212","published-online":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.3791\/67205","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,11]]}}}