{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:44:17Z","timestamp":1792601057778,"version":"4.5.0"},"reference-count":20,"publisher":"MyJove Corporation","issue":"210","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/67283","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T20:00:39Z","timestamp":1723838439000},"source":"Crossref","is-referenced-by-count":0,"title":["<em>In Vivo<\/em> Thoracic Dorsal Root Ganglia (DRG) Calcium Imaging and ECG Recording for Studying Peripheral Nerve Stimulation"],"prefix":"10.3791","author":[{"given":"Xia","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longhua","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2024,8,16]]},"reference":[{"issue":"19","key":"ref1","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1161\/CIRCULATIONAHA.123.065390","type":"journal-article","article-title":"Piezo1-mediated neurogenic inflammatory cascade exacerbates ventricular remodeling after myocardial infarction","volume":"149","author":"Sun","year":"2024","unstructured":"Sun, M. et al. Piezo1-mediated neurogenic inflammatory cascade exacerbates ventricular remodeling after myocardial infarction. Circulation. 149 (19), 1516-1533 (2024).","journal-title":"Circulation"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1007\/s12264-022-00841-w","type":"journal-article","article-title":"Referred somatic hyperalgesia mediates cardiac regulation by the activation of sympathetic nerves in a rat model of myocardial ischemia","volume":"38","author":"Cui","year":"2022","unstructured":"Cui, X. et al. Referred somatic hyperalgesia mediates cardiac regulation by the activation of sympathetic nerves in a rat model of myocardial ischemia. Neurosci Bull. 38 (4), 386-402 (2022).","journal-title":"Neurosci Bull"},{"issue":"7954","key":"ref3","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1038\/s41586-023-05828-9","type":"journal-article","article-title":"Fast and sensitive GCaMP calcium indicators for imaging neural populations","volume":"615","author":"Zhang","year":"2023","unstructured":"Zhang, Y. et al. Fast and sensitive GCaMP calcium indicators for imaging neural populations. Nature. 615 (7954), 884-891 (2023).","journal-title":"Nature"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","DOI":"10.1523\/ENEURO.0494-19.2020","type":"journal-article","article-title":"Mapping of sensory nerve subsets within the vagal ganglia and the brainstem using reporter mice for Pirt, TRPV1, 5-HT3, and Tac1 expression","volume":"7","author":"Kim","year":"2020","unstructured":"Kim, S. H. et al. Mapping of sensory nerve subsets within the vagal ganglia and the brainstem using reporter mice for Pirt, TRPV1, 5-HT3, and Tac1 expression. eNeuro. 7 (2), ENEURO.0494-19 (2020).","journal-title":"eNeuro"},{"issue":"5-6","key":"ref5","doi-asserted-by":"crossref","first-page":"458","DOI":"10.3109\/13550280903473460","type":"journal-article","article-title":"Advanced tracing tools: Functional neuronal expression of virally encoded fluorescent calcium indicator proteins","volume":"15","author":"Rothermel","year":"2009","unstructured":"Rothermel, M. et al. Advanced tracing tools: Functional neuronal expression of virally encoded fluorescent calcium indicator proteins. J Neurovirol. 15 (5-6), 458-464 (2009).","journal-title":"J Neurovirol"},{"issue":"192","key":"ref6","first-page":"e64826","type":"journal-article","article-title":"In vivo calcium imaging of neuronal ensembles in networks of primary sensory neurons in intact dorsal root ganglia","author":"Shannonhouse","year":"2023","unstructured":"Shannonhouse, J., Gomez, R., Son, H., Zhang, Y., Kim, Y. S. In vivo calcium imaging of neuronal ensembles in networks of primary sensory neurons in intact dorsal root ganglia. J Vis Exp. 192, e64826 (2023).","journal-title":"J Vis Exp"},{"issue":"205","key":"ref7","first-page":"e65975","type":"journal-article","article-title":"In vivo calcium imaging of dorsal root ganglia neurons' response to somatic and visceral stimuli","author":"Liu","year":"2024","unstructured":"Liu, K. et al. In vivo calcium imaging of dorsal root ganglia neurons' response to somatic and visceral stimuli. J Vis Exp. 205, e65975 (2024).","journal-title":"J Vis Exp"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1007\/BF02848784","type":"journal-article","article-title":"Dorsal root ganglion: The target of acupuncture in the treatment of asthma","volume":"24","author":"Feng","year":"2007","unstructured":"Feng, J. T., Hu, C. P., Li, X. Z. Dorsal root ganglion: The target of acupuncture in the treatment of asthma. Adv Ther. 24 (3), 598-602 (2007).","journal-title":"Adv Ther"},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","first-page":"R655","DOI":"10.1152\/ajpregu.00405.2017","type":"journal-article","article-title":"Role of TRPV1 in acupuncture modulation of reflex excitatory cardiovascular responses","volume":"314","author":"Guo","year":"2018","unstructured":"Guo, Z. L. et al. Role of TRPV1 in acupuncture modulation of reflex excitatory cardiovascular responses. Am J Physiol Regul Integr Comp Physiol. 314 (5), R655-r666 (2018).","journal-title":"Am J Physiol Regul Integr Comp Physiol"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s11655-023-3692-2","type":"journal-article","article-title":"Perspective of calcium imaging technology applied to acupuncture research","volume":"30","author":"Li","year":"2024","unstructured":"Li, S. et al. Perspective of calcium imaging technology applied to acupuncture research. Chin J Integr Med. 30 (1), 3-9 (2024).","journal-title":"Chin J Integr Med"},{"issue":"5","key":"ref11","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1016\/j.neuron.2016.07.044","type":"journal-article","article-title":"Coupled activation of primary sensory neurons contributes to chronic pain","volume":"91","author":"Kim","year":"2016","unstructured":"Kim, Y. S. et al. Coupled activation of primary sensory neurons contributes to chronic pain. Neuron. 91 (5), 1085-1096 (2016).","journal-title":"Neuron"},{"issue":"2","key":"ref12","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.neuron.2021.10.019","type":"journal-article","article-title":"Synchronized cluster firing, a distinct form of sensory neuron activation, drives spontaneous pain","volume":"110","author":"Zheng","year":"2022","unstructured":"Zheng, Q. et al. Synchronized cluster firing, a distinct form of sensory neuron activation, drives spontaneous pain. Neuron. 110 (2), 209-220.e206 (2022).","journal-title":"Neuron"},{"issue":"4","key":"ref13","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1097\/j.pain.0000000000002096","type":"journal-article","article-title":"Calcium imaging in population of dorsal root ganglion neurons unravels novel mechanisms of visceral pain sensitization and referred somatic hypersensitivity","volume":"162","author":"Gao","year":"2021","unstructured":"Gao, X. et al. Calcium imaging in population of dorsal root ganglion neurons unravels novel mechanisms of visceral pain sensitization and referred somatic hypersensitivity. Pain. 162 (4), 1068-1081 (2021).","journal-title":"Pain"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"9920","DOI":"10.1038\/s41598-022-14195-w","type":"journal-article","article-title":"TGR5 agonists induce peripheral and central hypersensitivity to bladder distension","volume":"12","author":"Caldwell","year":"2022","unstructured":"Caldwell, A. et al. TGR5 agonists induce peripheral and central hypersensitivity to bladder distension. Sci Rep. 12 (1), 9920 (2022).","journal-title":"Sci Rep"},{"issue":"12","key":"ref15","doi-asserted-by":"crossref","first-page":"8261","DOI":"10.1126\/sciadv.abd8261","type":"journal-article","article-title":"Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target","volume":"7","author":"Okada","year":"2021","unstructured":"Okada, T. et al. Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target. Sci Adv. 7 (12), 8261 (2021).","journal-title":"Sci Adv"},{"issue":"9","key":"ref16","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1016\/j.cell.2022.04.001","type":"journal-article","article-title":"Top-down control of hippocampal signal-to-noise by prefrontal long-range inhibition","volume":"185","author":"Malik","year":"2022","unstructured":"Malik, R., Li, Y., Schamiloglu, S., Sohal, V. S. Top-down control of hippocampal signal-to-noise by prefrontal long-range inhibition. Cell. 185 (9), 1602-1617.e17 (2022).","journal-title":"Cell"},{"issue":"8","key":"ref17","doi-asserted-by":"crossref","first-page":"1636","DOI":"10.1097\/j.pain.0000000000002556","type":"journal-article","article-title":"Purinergic signaling between neurons and satellite glial cells of mouse dorsal root ganglia modulates neuronal excitability in vivo","volume":"163","author":"Chen","year":"2022","unstructured":"Chen, Z. et al. Purinergic signaling between neurons and satellite glial cells of mouse dorsal root ganglia modulates neuronal excitability in vivo. Pain. 163 (8), 1636-1647 (2022).","journal-title":"Pain"},{"issue":"9","key":"ref18","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1038\/s41583-020-0333-z","type":"journal-article","article-title":"Emerging importance of satellite glia in nervous system function and dysfunction","volume":"21","author":"Hanani","year":"2020","unstructured":"Hanani, M., Spray, D. C. Emerging importance of satellite glia in nervous system function and dysfunction. Nat Rev Neurosci. 21 (9), 485-498 (2020).","journal-title":"Nat Rev Neurosci"},{"issue":"34","key":"ref19","doi-asserted-by":"crossref","first-page":"11890","DOI":"10.1523\/JNEUROSCI.0698-12.2012","type":"journal-article","article-title":"Differential expression and pharmacology of native P2X receptors in rat and primate sensory neurons","volume":"32","author":"Serrano","year":"2012","unstructured":"Serrano, A. et al. Differential expression and pharmacology of native P2X receptors in rat and primate sensory neurons. J Neurosci. 32 (34), 11890-11896 (2012).","journal-title":"J Neurosci"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"3087","DOI":"10.1038\/s41467-019-11158-0","type":"journal-article","article-title":"Long-term imaging of dorsal root ganglia in awake behaving mice","volume":"10","author":"Chen","year":"2019","unstructured":"Chen, C. et al. Long-term imaging of dorsal root ganglia in awake behaving mice. Nat Commun. 10 (1), 3087 (2019).","journal-title":"Nat Commun"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/67283\/in-vivo-thoracic-dorsal-root-ganglia-drg-calcium-imaging-ecg","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:09:44Z","timestamp":1792598984000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/67283\/in-vivo-thoracic-dorsal-root-ganglia-drg-calcium-imaging-ecg"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,16]]},"references-count":20,"journal-issue":{"issue":"210","published-online":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.3791\/67283","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,16]]}}}