{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:29:59Z","timestamp":1792636199796,"version":"4.5.0"},"reference-count":11,"publisher":"MyJove Corporation","issue":"215","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/67374","type":"journal-article","created":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T19:01:14Z","timestamp":1737745274000},"source":"Crossref","is-referenced-by-count":1,"title":["A Macrophage-Tumor Spheroid Co-Invasion Assay"],"prefix":"10.3791","author":[{"given":"Sven","family":"Hey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Linder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,1,24]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1007\/s40828-022-00163-4","type":"journal-article","article-title":"Macrophages: shapes and functions","volume":"8","author":"Lendeckel","year":"2022","unstructured":"Lendeckel, U., Venz, S., Wolke, C. Macrophages: shapes and functions. ChemTexts. 8 (2), 12 (2022).","journal-title":"ChemTexts"},{"issue":"39","key":"ref2","doi-asserted-by":"crossref","first-page":"8203","DOI":"10.2174\/0929867328666210720143721","type":"journal-article","article-title":"The role of tumor associated macrophages (TAMs) in cancer progression, chemoresistance, angiogenesis and metastasis - Current status","volume":"28","author":"Dallavalasa","year":"2021","unstructured":"Dallavalasa, S. et al. The role of tumor associated macrophages (TAMs) in cancer progression, chemoresistance, angiogenesis and metastasis - Current status. Curr Med Chem. 28 (39), 8203-8236 (2021).","journal-title":"Curr Med Chem"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"583084","DOI":"10.3389\/fimmu.2020.583084","type":"journal-article","article-title":"Tumor-associated macrophages in tumor immunity","volume":"11","author":"Pan","year":"2020","unstructured":"Pan, Y., Yu, Y., Wang, X., Zhang, T. Tumor-associated macrophages in tumor immunity. Front Immunol. 11, 583084 (2020).","journal-title":"Front Immunol"},{"issue":"13","key":"ref4","doi-asserted-by":"crossref","first-page":"6995","DOI":"10.3390\/ijms22136995","type":"journal-article","article-title":"Macrophage Polarization States in the Tumor Microenvironment","volume":"22","author":"Boutilier","year":"2021","unstructured":"Boutilier, A. J., Elsawa, S. F. Macrophage Polarization States in the Tumor Microenvironment. Int J Mol Sci. 22 (13), 6995 (2021).","journal-title":"Int J Mol Sci"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1038\/s41467-024-45558-8","type":"journal-article","article-title":"Intercellular transfer of cancer cell invasiveness via endosome-mediated protease shedding","volume":"15","author":"Wenzel","year":"2024","unstructured":"Wenzel, E. M. et al. Intercellular transfer of cancer cell invasiveness via endosome-mediated protease shedding. Nat Commun. 15 (1), 1277 (2024).","journal-title":"Nat Commun"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/978-1-0716-2914-7_7","type":"journal-article","article-title":"In vivo analysis of tumor-associated macrophages in the tumor microenvironment","volume":"2614","author":"Anfray","year":"2023","unstructured":"Anfray, C., Ummarino, A., Calvo, A., Allavena, P., Torres Andon, F. In vivo analysis of tumor-associated macrophages in the tumor microenvironment. Methods Mol Biol. 2614, 93-108 (2023).","journal-title":"Methods Mol Biol"},{"issue":"11","key":"ref7","doi-asserted-by":"crossref","first-page":"e202302158","DOI":"10.26508\/lsa.202302158","type":"journal-article","article-title":"KIF16B drives MT1-MMP recycling in macrophages and promotes co-invasion of cancer cells","volume":"6","author":"Hey","year":"2023","unstructured":"Hey, S., Wiesner, C., Barcelona, B., Linder, S. KIF16B drives MT1-MMP recycling in macrophages and promotes co-invasion of cancer cells. Life Sci Alliance. 6 (11), e202302158 (2023).","journal-title":"Life Sci Alliance"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"100899","DOI":"10.1016\/j.tranon.2020.100899","type":"journal-article","article-title":"Plasticity of cancer cell invasion: Patterns and mechanisms","volume":"14","author":"Wu","year":"2021","unstructured":"Wu, J. S. et al. Plasticity of cancer cell invasion: Patterns and mechanisms. Transl Oncol. 14 (1), 100899 (2021).","journal-title":"Transl Oncol"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1038\/s41392-024-01885-2","type":"journal-article","article-title":"Mechanism insights and therapeutic intervention of tumor metastasis: latest developments and perspectives","volume":"9","author":"Shi","year":"2024","unstructured":"Shi, X. et al. Mechanism insights and therapeutic intervention of tumor metastasis: latest developments and perspectives. Signal Transduct Target Ther. 9 (1), 192 (2024).","journal-title":"Signal Transduct Target Ther"},{"issue":"5","key":"ref10","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1089\/ten.2006.0251","type":"journal-article","article-title":"Genomic and morphological changes of neuroblastoma cells in response to three-dimensional matrices","volume":"13","author":"Li","year":"2007","unstructured":"Li, G. N., Livi, L. L., Gourd, C. M., Deweerd, E. S., Hoffman-Kim, D. Genomic and morphological changes of neuroblastoma cells in response to three-dimensional matrices. Tissue Eng. 13 (5), 1035-1047(2007).","journal-title":"Tissue Eng"},{"key":"ref11","first-page":"e61392","type":"journal-article","article-title":"Monitoring cancer cell invasion and T-cell cytotoxicity in 3D culture","volume":"160","author":"Lin","year":"2020","unstructured":"Lin, Y. N. et al. Monitoring cancer cell invasion and T-cell cytotoxicity in 3D culture. J Vis Exp. 160, e61392 (2020).","journal-title":"J Vis Exp"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/67374\/a-macrophage-tumor-spheroid-co-invasion-assay","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:44:10Z","timestamp":1792597450000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/67374\/a-macrophage-tumor-spheroid-co-invasion-assay"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,24]]},"references-count":11,"journal-issue":{"issue":"215","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/67374","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,24]]}}}