{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:38:29Z","timestamp":1792600709715,"version":"4.5.0"},"reference-count":38,"publisher":"MyJove Corporation","issue":"216","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/67539","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T19:00:22Z","timestamp":1740164422000},"source":"Crossref","is-referenced-by-count":0,"title":["The Creation of a Rat Model for Osteosarcopenia via Ovariectomy"],"prefix":"10.3791","author":[{"given":"Xiaoping","family":"Ma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijian","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siwei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaosheng","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaonan","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,2,21]]},"reference":[{"issue":"4","key":"ref1","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.jocd.2009.06.004","type":"journal-article","article-title":"Beyond FRAX\u00ae: It's time to consider "Sarco-Osteopenia","volume":"12","author":"Binkley","year":"2009","unstructured":"Binkley, N., Buehring, B. Beyond FRAX\u00ae: It's time to consider \"Sarco-Osteopenia J Clin Densitom. 12 (4), 413-416 (2009).","journal-title":"J Clin Densitom"},{"issue":"10","key":"ref2","doi-asserted-by":"crossref","first-page":"2781","DOI":"10.1007\/s00198-017-4151-8","type":"journal-article","article-title":"Osteosarcopenia: where bone, muscle, and fat collide","volume":"28","author":"Hirschfeld","year":"2017","unstructured":"Hirschfeld, H. P., Kinsella, R., Duque, G. Osteosarcopenia: where bone, muscle, and fat collide. Osteoporos Int. 28 (10), 2781-2790 (2017).","journal-title":"Osteoporos Int"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"e164604","DOI":"10.1001\/jamasurg.2016.4604","type":"journal-article","article-title":"Association of radiologic indicators of frailty with 1-year mortality in older trauma patients","volume":"152","author":"Kaplan","year":"2017","unstructured":"Kaplan, S. J. et al. Association of radiologic indicators of frailty with 1-year mortality in older trauma patients. JAMA Surg. 152 (2), e164604 (2017).","journal-title":"JAMA Surg"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1007\/s41999-018-0079-6","type":"journal-article","article-title":"Sarcopenia and osteoporosis in older people: a systematic review and meta-analysis","volume":"9","author":"Nielsen","year":"2018","unstructured":"Nielsen, B. R., Abdulla, J., Andersen, H. E., Schwarz, P., Suetta, C. Sarcopenia and osteoporosis in older people: a systematic review and meta-analysis. Eur Geriatr Med. 9 (4), 419-434 (2018).","journal-title":"Eur Geriatr Med"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1093\/ageing\/afq034","type":"journal-article","article-title":"Sarcopenia: European consensus on definition and diagnosis: Report of the European Working Group on sarcopenia in older people","volume":"39","author":"Cruz-Jentoft","year":"2010","unstructured":"Cruz-Jentoft, A. J. et al. Sarcopenia: European consensus on definition and diagnosis: Report of the European Working Group on sarcopenia in older people. Age Ageing. 39 (4), 412-423 (2010).","journal-title":"Age Ageing"},{"issue":"10","key":"ref6","doi-asserted-by":"crossref","first-page":"5591","DOI":"10.3390\/ijms23105591","type":"journal-article","article-title":"Osteosarcopenia: A narrative review on clinical studies","volume":"23","author":"Polito","year":"2022","unstructured":"Polito, A., Barnaba, L., Ciarapica, D., Azzini, E. Osteosarcopenia: A narrative review on clinical studies. Int J Mol Sci. 23 (10), 5591 (2022).","journal-title":"Int J Mol Sci"},{"issue":"9","key":"ref7","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.3390\/nu16091328","type":"journal-article","article-title":"Three-year mortality of older hospitalized patients with osteosarcopenia: Data from the OsteoSys study","volume":"16","author":"Pourhassan","year":"2024","unstructured":"Pourhassan, M. et al. Three-year mortality of older hospitalized patients with osteosarcopenia: Data from the OsteoSys study. Nutrients. 16 (9), 1328 (2024).","journal-title":"Nutrients"},{"issue":"4","key":"ref8","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/j.jamda.2020.07.032","type":"journal-article","article-title":"Osteosarcopenia predicts falls, fractures, and mortality in Chilean community-dwelling older adults","volume":"22","author":"Salech","year":"2021","unstructured":"Salech, F. et al. Osteosarcopenia predicts falls, fractures, and mortality in Chilean community-dwelling older adults. J Am Med Dir Assoc. 22 (4), 853-858 (2021).","journal-title":"J Am Med Dir Assoc"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.11005\/jbm.2018.25.1.1","type":"journal-article","article-title":"Review of epidemiology, diagnosis, and treatment of osteosarcopenia in Korea","volume":"25","author":"Yoo","year":"2018","unstructured":"Yoo, J. I., Ha, Y. C. Review of epidemiology, diagnosis, and treatment of osteosarcopenia in Korea. J Bone Metab. 25 (1), 1-7 (2018).","journal-title":"J Bone Metab"},{"issue":"2","key":"ref10","doi-asserted-by":"crossref","first-page":"291","DOI":"10.3390\/nu13020291","type":"journal-article","article-title":"Related factors and clinical outcomes of osteosarcopenia: A narrative review","volume":"13","author":"Inoue","year":"2021","unstructured":"Inoue, T. et al. Related factors and clinical outcomes of osteosarcopenia: A narrative review. Nutrients. 13 (2), 291 (2021).","journal-title":"Nutrients"},{"issue":"11","key":"ref11","doi-asserted-by":"crossref","first-page":"2173","DOI":"10.1007\/s00198-021-05963-x","type":"journal-article","article-title":"The analysis of osteosarcopenia as a risk factor for fractures, mortality, and falls","volume":"32","author":"Teng","year":"2021","unstructured":"Teng, Z. et al. The analysis of osteosarcopenia as a risk factor for fractures, mortality, and falls. Osteoporos Int. 32 (11), 2173-2183 (2021).","journal-title":"Osteoporos Int"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1002\/jcsm.12588","type":"journal-article","article-title":"Hallmarks of frailty and osteosarcopenia in prematurely aged PolgA(D257A\/D257A) mice","volume":"11","author":"Scheuren","year":"2020","unstructured":"Scheuren, A. C. et al. Hallmarks of frailty and osteosarcopenia in prematurely aged PolgA(D257A\/D257A) mice. J Cachexia Sarcopenia Muscle. 11 (4), 1121-1140 (2020).","journal-title":"J Cachexia Sarcopenia Muscle"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.steroids.2016.10.008","type":"journal-article","article-title":"Continuous corticosterone delivery via the drinking water or pellet implantation: A comparative study in mice","volume":"116","author":"Gasparini","year":"2016","unstructured":"Gasparini, S. J. et al. Continuous corticosterone delivery via the drinking water or pellet implantation: A comparative study in mice. Steroids. 116, 76-82 (2016).","journal-title":"Steroids"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1038\/s41598-019-56853-6","type":"journal-article","article-title":"A butanolic fraction from the standardized stem extract of Cassia occidentalis L delivered by a self-emulsifying drug delivery system protects rats from glucocorticoid-induced osteopenia and muscle atrophy","volume":"10","author":"Pal","year":"2020","unstructured":"Pal, S. et al. A butanolic fraction from the standardized stem extract of Cassia occidentalis L delivered by a self-emulsifying drug delivery system protects rats from glucocorticoid-induced osteopenia and muscle atrophy. Sci Rep. 10 (1), 195 (2020).","journal-title":"Sci Rep"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"e55789","DOI":"10.1371\/journal.pone.0055789","type":"journal-article","article-title":"Mitochondrial DNA mutations in mutator mice confer respiration defects and B-cell lymphoma development","volume":"8","author":"Mito","year":"2013","unstructured":"Mito, T. et al. Mitochondrial DNA mutations in mutator mice confer respiration defects and B-cell lymphoma development. PLoS One. 8 (2), e55789 (2013).","journal-title":"PLoS One"},{"issue":"10","key":"ref16","first-page":"915","type":"journal-article","article-title":"Cancellous bone structure of iliac crest biopsies following 370 days of head-down bed rest","volume":"76","author":"Thomsen","year":"2005","unstructured":"Thomsen, J. S. et al. Cancellous bone structure of iliac crest biopsies following 370 days of head-down bed rest. Aviat Space Environ Med. 76 (10), 915-922 (2005).","journal-title":"Aviat Space Environ Med"},{"issue":"12","key":"ref17","doi-asserted-by":"crossref","first-page":"4499","DOI":"10.3390\/nu13124499","type":"journal-article","article-title":"Exercise and nutrition impact on osteoporosis and sarcopenia-The incidence of osteosarcopenia: A narrative review","volume":"13","author":"Papadopoulou","year":"2021","unstructured":"Papadopoulou, S. K. et al. Exercise and nutrition impact on osteoporosis and sarcopenia-The incidence of osteosarcopenia: A narrative review. Nutrients. 13 (12), 4499 (2021).","journal-title":"Nutrients"},{"issue":"7","key":"ref18","doi-asserted-by":"crossref","first-page":"689","DOI":"10.3357\/ASEM.2984.2011","type":"journal-article","article-title":"A hind limb disuse model inducing extensor digitorum longus atrophy in rats: tail suspension-immobilization","volume":"82","author":"Du","year":"2011","unstructured":"Du, F. et al. A hind limb disuse model inducing extensor digitorum longus atrophy in rats: tail suspension-immobilization. Aviat Space Environ Med. 82 (7), 689-693 (2011).","journal-title":"Aviat Space Environ Med"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.lfs.2018.10.032","type":"journal-article","article-title":"Strength training reverses ovariectomy-induced bone loss and improve metabolic parameters in female Wistar rats","volume":"213","author":"Gomes","year":"2018","unstructured":"Gomes, R. M. et al. Strength training reverses ovariectomy-induced bone loss and improve metabolic parameters in female Wistar rats. Life Sci. 213, 134-141 (2018).","journal-title":"Life Sci"},{"issue":"1","key":"ref20","first-page":"1","type":"journal-article","article-title":"Establishment of a rat model of osteosarcopenia","volume":"28","author":"Ma","year":"2022","unstructured":"Ma, J. et al. Establishment of a rat model of osteosarcopenia. Chin J Osteoporos. 28 (1), 1-5+15 (2022).","journal-title":"Chin J Osteoporos"},{"issue":"1","key":"ref21","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1186\/s12877-023-04085-9","type":"journal-article","article-title":"Prevalence and risk factors of osteosarcopenia: a systematic review and meta-analysis","volume":"23","author":"Huang","year":"2023","unstructured":"Huang, T. et al. Prevalence and risk factors of osteosarcopenia: a systematic review and meta-analysis. BMC Geriatr. 23 (1), 369 (2023).","journal-title":"BMC Geriatr"},{"issue":"11","key":"ref22","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1007\/s40520-019-01266-8","type":"journal-article","article-title":"mineral density and trabecular bone score in Chinese subjects with sarcopenia","volume":"31","author":"Qi","year":"2019","unstructured":"Qi, H. et al. Bone mineral density and trabecular bone score in Chinese subjects with sarcopenia. Aging Clin Exp Res. 31 (11), 1549-1556 (2019).","journal-title":"Aging Clin Exp Res"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1073587","DOI":"10.3389\/fendo.2023.1073587","type":"journal-article","article-title":"An integrated study of hormone-related sarcopenia for modeling and comparative transcriptome in rats","volume":"14","author":"Shu","year":"2023","unstructured":"Shu, H. et al. An integrated study of hormone-related sarcopenia for modeling and comparative transcriptome in rats. Front Endocrinol. 14, 1073587 (2023).","journal-title":"Front Endocrinol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.nut.2018.09.001","type":"journal-article","article-title":"Influence of dietary vitamin D deficiency on bone strength, body composition, and muscle in ovariectomized rats fed a high-fat diet","volume":"60","author":"Nakaoka","year":"2019","unstructured":"Nakaoka, K., Yamada, A., Noda, S., Goseki-Sone, M. Influence of dietary vitamin D deficiency on bone strength, body composition, and muscle in ovariectomized rats fed a high-fat diet. Nutrition. 60, 87-93 (2019).","journal-title":"Nutrition"},{"key":"ref25","type":"book","article-title":"Castration method was used to construct a rat model of kidney-yang deficiency sarcopeniaosteoporosis and explore the mechanism","volume-title":"Chin Arch Tradit Chin Med","author":"Chong","year":"2024","unstructured":"Chong, L., Xiaonan, Q., Hao, Z., Xiaosheng, Y. Castration method was used to construct a rat model of kidney-yang deficiency sarcopeniaosteoporosis and explore the mechanism. Chin Arch Tradit Chin Med. (2024)."},{"issue":"15","key":"ref26","doi-asserted-by":"crossref","first-page":"2239","DOI":"10.2174\/0113862073263020231220043405","type":"journal-article","article-title":"(S)-10-hydroxycamptothecin inhibits EMT-evoked osteosarcoma cell growth and metastasis by activating the HIPPO signaling pathway","volume":"27","author":"Ma","year":"2024","unstructured":"Ma, X. et al. (S)-10-hydroxycamptothecin inhibits EMT-evoked osteosarcoma cell growth and metastasis by activating the HIPPO signaling pathway. Combin Chem High Throughput Screen. 27 (15), 2239-2248 (2024).","journal-title":"Combin Chem High Throughput Screen"},{"issue":"4","key":"ref27","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/jbmr.5650040104","type":"journal-article","article-title":"Characteristics of an ovariectomized osteopenic rat model","volume":"4","author":"Yamazaki","year":"1989","unstructured":"Yamazaki, I., Yamaguchi, H. Characteristics of an ovariectomized osteopenic rat model. J Bone Miner Res. 4 (4), 13-22 (1989).","journal-title":"J Bone Miner Res"},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"4881","DOI":"10.18632\/aging.202617","type":"journal-article","article-title":"MiR-141-3p promotes mitochondrial dysfunction in ovariectomy-induced sarcopenia via targeting Fkbp5 and Fibin","volume":"13","author":"Lee","year":"2021","unstructured":"Lee, H. et al. MiR-141-3p promotes mitochondrial dysfunction in ovariectomy-induced sarcopenia via targeting Fkbp5 and Fibin. Aging (Albany NY). 13 (4), 4881-4894 (2021).","journal-title":"Aging (Albany NY)"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.bone.2017.08.005","type":"journal-article","article-title":"Globular adiponectin reverses osteo-sarcopenia and altered body composition in ovariectomized rats","volume":"105","author":"China","year":"2017","unstructured":"China, S. P. et al. Globular adiponectin reverses osteo-sarcopenia and altered body composition in ovariectomized rats. Bone. 105, 75-86 (2017).","journal-title":"Bone"},{"issue":"3","key":"ref30","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/8756-3282(93)90166-8","type":"journal-article","article-title":"Animal models for the study of bone loss after cessation of ovarian function","volume":"14","author":"Rodgers","year":"1993","unstructured":"Rodgers, J. B., Monier-Faugere, M. C., Malluche, H. Animal models for the study of bone loss after cessation of ovarian function. Bone. 14 (3), 369-377 (1993).","journal-title":"Bone"},{"issue":"2","key":"ref31","first-page":"276","type":"journal-article","article-title":"Age-related changes in bone mass in the senescence-accelerated mouse (SAM). SAM-R\/3 and SAM-P\/6 as new murine models for senile osteoporosis","volume":"125","author":"Matsushita","year":"1986","unstructured":"Matsushita, M. et al. Age-related changes in bone mass in the senescence-accelerated mouse (SAM). SAM-R\/3 and SAM-P\/6 as new murine models for senile osteoporosis. Am J Pathol. 125 (2), 276-283 (1986).","journal-title":"Am J Pathol"},{"issue":"2","key":"ref32","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.jep.2011.09.030","type":"journal-article","article-title":"A traditional Chinese herbal preparation, Er-Zhi-Wan, prevent ovariectomy-induced osteoporosis in rats","volume":"138","author":"Cheng","year":"2011","unstructured":"Cheng, M. et al. A traditional Chinese herbal preparation, Er-Zhi-Wan, prevent ovariectomy-induced osteoporosis in rats. J Ethnopharmacol. 138 (2), 279-285 (2011).","journal-title":"J Ethnopharmacol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"556581","DOI":"10.3389\/fendo.2020.556581","type":"journal-article","article-title":"Ostarine and ligandrol improve muscle tissue in an ovariectomized rat model","volume":"11","author":"Roch","year":"2020","unstructured":"Roch, P. J. et al. Ostarine and ligandrol improve muscle tissue in an ovariectomized rat model. Front Endocrinol. 11, 556581 (2020).","journal-title":"Front Endocrinol"},{"issue":"3","key":"ref34","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.joca.2019.06.014","type":"journal-article","article-title":"Raloxifene retards cartilage degradation and improves subchondral bone microarchitecture in ovariectomized rats with patella baja-induced - patellofemoral joint osteoarthritis","volume":"28","author":"Bei","year":"2020","unstructured":"Bei, M. J. et al. Raloxifene retards cartilage degradation and improves subchondral bone microarchitecture in ovariectomized rats with patella baja-induced - patellofemoral joint osteoarthritis. Osteoarthritis Cartilage. 28 (3), 344-355 (2020).","journal-title":"Osteoarthritis Cartilage"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"535310","DOI":"10.3389\/fcimb.2020.535310","type":"journal-article","article-title":"Fecal and serum metabolomic signatures and microbial community profiling of postmenopausal osteoporosis mice model","volume":"10","author":"Wen","year":"2020","unstructured":"Wen, K. et al. Fecal and serum metabolomic signatures and microbial community profiling of postmenopausal osteoporosis mice model. Front Cell Infect Microbiol. 10, 535310 (2020).","journal-title":"Front Cell Infect Microbiol"},{"issue":"4","key":"ref36","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1002\/jbm.a.36294","type":"journal-article","article-title":"Multiscale characterization of cortical bone composition, microstructure, and nanomechanical properties in experimentally induced osteoporosis","volume":"106","author":"Shah","year":"2018","unstructured":"Shah, F. A., Stoica, A., Cardemil, C., Palmquist, A. Multiscale characterization of cortical bone composition, microstructure, and nanomechanical properties in experimentally induced osteoporosis. J Biomed Mater Res A. 106 (4), 997-1007 (2018).","journal-title":"J Biomed Mater Res A"},{"issue":"1","key":"ref37","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1590\/S0102-67202012000100011","type":"journal-article","article-title":"Rat's age versus human's age: what is the relationship","volume":"25","author":"Andreollo","year":"2012","unstructured":"Andreollo, N. A., Santos, E. F., Ara\u00fajo, M. R., Lopes, L. R. Rat's age versus human's age: what is the relationship? Arq Bras Cir Dig. 25 (1), 49-51 (2012).","journal-title":"Arq Bras Cir Dig"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"112159","DOI":"10.1016\/j.exger.2023.112159","type":"journal-article","article-title":"Thigh muscles are more susceptible to age-related muscle loss when compared to lower leg and pelvic muscles","volume":"175","author":"Fuchs","year":"2023","unstructured":"Fuchs, C. J. et al. Thigh muscles are more susceptible to age-related muscle loss when compared to lower leg and pelvic muscles. Exp Gerontol. 175, 112159 (2023).","journal-title":"Exp Gerontol"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/67539\/the-creation-of-a-rat-model-for-osteosarcopenia-via-ovariectomy","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:40:20Z","timestamp":1792597220000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/67539\/the-creation-of-a-rat-model-for-osteosarcopenia-via-ovariectomy"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,21]]},"references-count":38,"journal-issue":{"issue":"216","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/67539","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,21]]}}}