{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:38:54Z","timestamp":1792600734747,"version":"4.5.0"},"reference-count":19,"publisher":"MyJove Corporation","issue":"215","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/67597","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T19:00:24Z","timestamp":1737140424000},"source":"Crossref","is-referenced-by-count":1,"title":["Long-Term Live Imaging of &lt;i&gt;Drosophila&lt;\/i&gt; Pupal Leg Development After Puparium Removal"],"prefix":"10.3791","author":[{"given":"Shotaro","family":"Hiraiwa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Kojima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,1,17]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1083\/jcb.200905110","type":"journal-article","article-title":"Temporal regulation of Drosophila IAP1 determines caspase functions in sensory organ development","volume":"187","author":"Koto","year":"2009","unstructured":"Koto, A., Kuranaga, E., Miura, M. Temporal regulation of Drosophila IAP1 determines caspase functions in sensory organ development. J Cell Biol. 187 (2), 219-231 (2009).","journal-title":"J Cell Biol"},{"issue":"12","key":"ref2","doi-asserted-by":"crossref","first-page":"3074","DOI":"10.1038\/nprot.2007.417","type":"journal-article","article-title":"Live imaging of epidermal morphogenesis during the development of the adult abdominal epidermis of Drosophila","volume":"2","author":"Ninov","year":"2007","unstructured":"Ninov, N., Mart\u00edn-Blanco, E. Live imaging of epidermal morphogenesis during the development of the adult abdominal epidermis of Drosophila. Nat Protoc. 2 (12), 3074-3080 (2007).","journal-title":"Nat Protoc"},{"issue":"11","key":"ref3","doi-asserted-by":"crossref","first-page":"1676","DOI":"10.1021\/acscentsci.9b00678","type":"journal-article","article-title":"Activatable photosensitizer for targeted ablation of lacZ-positive cells with single-cell resolution","volume":"5","author":"Chiba","year":"2019","unstructured":"Chiba, M. et al. Activatable photosensitizer for targeted ablation of lacZ-positive cells with single-cell resolution. ACS Cent Sci. 5 (11), 1676-1681 (2019).","journal-title":"ACS Cent Sci"},{"issue":"16","key":"ref4","doi-asserted-by":"crossref","first-page":"1798","DOI":"10.1016\/j.cub.2014.06.052","type":"journal-article","article-title":"Local increases in mechanical tension shape compartment boundaries by biasing cell intercalations","volume":"24","author":"Umetsu","year":"2014","unstructured":"Umetsu, D. et al. Local increases in mechanical tension shape compartment boundaries by biasing cell intercalations. Curr Biol. 24 (16), 1798-1805 (2014).","journal-title":"Curr Biol"},{"issue":"6","key":"ref5","first-page":"568","type":"journal-article","article-title":"Programmed disassembly of a microtubule-based membrane protrusion network coordinates 3D epithelial morphogenesis in Drosophila","volume":"43","author":"Tran","year":"2024","unstructured":"Tran, N. V. et al. Programmed disassembly of a microtubule-based membrane protrusion network coordinates 3D epithelial morphogenesis in Drosophila. EMBO J. 43 (6), 568-594 (2024).","journal-title":"EMBO J"},{"issue":"95","key":"ref6","first-page":"e52120","type":"journal-article","article-title":"Live-imaging of the Drosophila pupal eye","author":"Hellerman","year":"2015","unstructured":"Hellerman, M. B., Choe, R. H., Johnson, R. I. Liveimaging of the Drosophila pupal eye. J Vis Exp. 95, e52120 (2015).","journal-title":"J Vis Exp"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"101396","DOI":"10.1016\/j.xpro.2022.101396","type":"journal-article","article-title":"Mounting Drosophila pupae for laser ablation and live imaging of the dorsal thorax","volume":"3","author":"O'Connor","year":"2022","unstructured":"O'Connor, J. T., Shannon, E. K., Hutson, M. S., PageMcCaw, A. Mounting Drosophila pupae for laser ablation and live imaging of the dorsal thorax. STAR Protoc. 3 (2), 101396 (2022).","journal-title":"STAR Protoc"},{"issue":"51","key":"ref8","first-page":"e2706","type":"journal-article","article-title":"Live-cell imaging of sensory organ precursor cells in intact Drosophila pupae","author":"Ziserman","year":"2011","unstructured":"Ziserman, D., Roegiers, F. Live-cell imaging of sensory organ precursor cells in intact Drosophila pupae. J Vis Exp. 51, e2706 (2011).","journal-title":"J Vis Exp"},{"issue":"1-2","key":"ref9","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1387\/ijdb.120111ap","type":"journal-article","article-title":"In vivo imaging of Drosophila wing heart development during pupal stages","volume":"57","author":"T\u00f6gel","year":"2013","unstructured":"T\u00f6gel, M., Pass, G., Paululat, A. In vivo imaging of Drosophila wing heart development during pupal stages. Int J Dev Biol. 57 (1-2), 13-24 (2013).","journal-title":"Int J Dev Biol"},{"issue":"136","key":"ref10","first-page":"e57871","type":"journal-article","article-title":"Long-term in vivo tracking of inflammatory cell dynamics within Drosophila pupae","author":"Weavers","year":"2018","unstructured":"Weavers, H., Franz, A., Wood, W., Martin, P. Longterm in vivo tracking of inflammatory cell dynamics within Drosophila pupae. J Vis Exp. 136, e57871 (2018).","journal-title":"J Vis Exp"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.cois.2016.11.002","type":"journal-article","article-title":"Developmental mechanism of the tarsus in insect legs","volume":"19","author":"Kojima","year":"2017","unstructured":"Kojima, T. Developmental mechanism of the tarsus in insect legs. Curr Opin Insect Sci. 19, 36-42 (2017).","journal-title":"Curr Opin Insect Sci"},{"issue":"2","key":"ref12","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1006\/dbio.2002.0593","type":"journal-article","article-title":"Joint development in the Drosophila leg: Cell movements and cell populations","volume":"246","author":"Mirth","year":"2002","unstructured":"Mirth, C., Akam, M. Joint development in the Drosophila leg: Cell movements and cell populations. Dev Biol. 246 (2), 391-406 (2002).","journal-title":"Dev Biol"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.ydbio.2004.10.026","type":"journal-article","article-title":"Ecdysteroid control of metamorphosis in the differentiating adult leg structures of Drosophila melanogaster","volume":"278","author":"Mirth","year":"2005","unstructured":"Mirth, C. Ecdysteroid control of metamorphosis in the differentiating adult leg structures of Drosophila melanogaster. Dev Biol. 278 (1), 163-174 (2005).","journal-title":"Dev Biol"},{"issue":"12","key":"ref14","doi-asserted-by":"crossref","first-page":"2055","DOI":"10.1242\/dev.047175","type":"journal-article","article-title":"Dynamic shape changes of ECM-producing cells drive morphogenesis of ball-and-socket joints in the fly leg","volume":"137","author":"Tajiri","year":"2010","unstructured":"Tajiri, R., Misaki, K., Yonemura, S., Hayashi, S. Dynamic shape changes of ECM-producing cells drive morphogenesis of ball-and-socket joints in the fly leg. Development. 137 (12), 2055-2063 (2010).","journal-title":"Development"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.1007\/s00427-024-00719-z","type":"journal-article","article-title":"Unveiling the cell dynamics during the final shape formation of the tarsus in Drosophila adult leg by live imaging","author":"Hiraiwa","year":"2024","unstructured":"Hiraiwa, S. et al. Unveiling the cell dynamics during the final shape formation of the tarsus in Drosophila adult leg by live imaging. Dev Genes Evol. (2024).","journal-title":"Dev Genes Evol"},{"key":"ref16","volume-title":"ImageJ plugin page","year":"2024","unstructured":"Signaling RIKEN. ImageJ plugin page. Available at: https:\/\/signaling.riken.jp\/en\/en-tools\/imagej\/629\/ (2024)."},{"issue":"4","key":"ref17","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1242\/jcs.01663","type":"journal-article","article-title":"Epithelial re-organization and dynamics of progression through mitosis in Drosophila separase complex mutants","volume":"118","author":"Pandey","year":"2005","unstructured":"Pandey, R., Heidmann, S., Lehner, C. F. Epithelial re-organization and dynamics of progression through mitosis in Drosophila separase complex mutants. J Cell Sci. 118 (4), 733-742 (2005).","journal-title":"J Cell Sci"},{"issue":"2","key":"ref18","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1083\/jcb.149.2.471","type":"journal-article","article-title":"Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila","volume":"149","author":"Kiehart","year":"2000","unstructured":"Kiehart, D. P., Galbraith, C. G., Edwards, K. A., Rickoll, W. L., Montague, R. A. Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila. J Cell Biol. 149 (2), 471-490 (2000).","journal-title":"J Cell Biol"},{"key":"ref19","type":"book","volume-title":"Drosophila: A Laboratory Handbook","author":"Ashburner","year":"1989","unstructured":"Ashburner, M. Drosophila: A Laboratory Handbook. Cold Spring Harbor Laboratory Press (1989)."}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/67597\/long-term-live-imaging-drosophila-pupal-leg-development-after","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:45:13Z","timestamp":1792597513000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/67597\/long-term-live-imaging-drosophila-pupal-leg-development-after"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,17]]},"references-count":19,"journal-issue":{"issue":"215","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/67597","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,17]]}}}