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Stroke. 36 (7), 1551-1556 (2005).","journal-title":"Stroke"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.arr.2016.02.009","type":"journal-article","article-title":"Lysosomal cell death mechanisms in aging","volume":"32","author":"G\u00f3mez-Sintes","year":"2016","unstructured":"G\u00f3mez-Sintes, R., Ledesma, M. D., Boya, P. Lysosomal cell death mechanisms in aging. Ageing Res Rev. 32, 150-168 (2016).","journal-title":"Ageing Res Rev"},{"issue":"8","key":"ref53","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1038\/s41419-019-1790-z","type":"journal-article","article-title":"Raptinal bypasses BAX, BAK, and BOK for mitochondrial outer membrane permeabilization and intrinsic apoptosis","volume":"10","author":"Heimer","year":"2019","unstructured":"Heimer, S., Knoll, G., Schulze-Osthoff, K., Ehrenschwender, M. Raptinal bypasses BAX, BAK, and BOK for mitochondrial outer membrane permeabilization and intrinsic apoptosis. Cell Death Dis. 10 (8), 556 (2019).","journal-title":"Cell Death Dis"},{"issue":"3","key":"ref54","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1038\/s41419-018-0408-1","type":"journal-article","article-title":"Cytochrome c speeds up caspase cascade activation by blocking 14-3-3\u03b5-dependent Apaf-1 inhibition","volume":"9","author":"Elena-Real","year":"2018","unstructured":"Elena-Real, C. A. et al. Cytochrome c speeds up caspase cascade activation by blocking 14-3-3\u03b5-dependent Apaf-1 inhibition. Cell Death Dis. 9 (3), 365 (2018).","journal-title":"Cell Death Dis"},{"issue":"8","key":"ref55","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.1161\/01.STR.0000021001.18101.A5","type":"journal-article","article-title":"Clinical and vascular outcome in internal carotid artery versus middle cerebral artery occlusions after intravenous tissue plasminogen activator","volume":"33","author":"Linfante","year":"2002","unstructured":"Linfante, I. et al. Clinical and vascular outcome in internal carotid artery versus middle cerebral artery occlusions after intravenous tissue plasminogen activator. Stroke. 33 (8), 2066-2071 (2002).","journal-title":"Stroke"},{"issue":"2","key":"ref56","doi-asserted-by":"crossref","first-page":"E326","DOI":"10.1152\/ajpendo.00345.2001","type":"journal-article","article-title":"Increased muscle proteolysis after local trauma mainly reflects macrophage-associated lysosomal proteolysis","volume":"282","author":"Farges","year":"2002","unstructured":"Farges, M. C. et al. Increased muscle proteolysis after local trauma mainly reflects macrophage-associated lysosomal proteolysis. 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