{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:38:44Z","timestamp":1792600724005,"version":"4.5.0"},"reference-count":26,"publisher":"MyJove Corporation","issue":"215","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/67648","type":"journal-article","created":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:02:22Z","timestamp":1738368142000},"source":"Crossref","is-referenced-by-count":3,"title":["Isolation of Live Myeloid and Epithelial Cell Populations from the Mouse Lung"],"prefix":"10.3791","author":[{"given":"Daisy A.","family":"Hoagland","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth A.","family":"Franklin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,1,31]]},"reference":[{"issue":"6","key":"ref1","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1164\/ajrccm.154.6.8970383","type":"journal-article","article-title":"Mucociliary clearance in the airways","volume":"154","author":"Wanner","year":"1996","unstructured":"Wanner, A., Salath\u00e9, M., O'Riordan, T. G. Mucociliary clearance in the airways. Am J Respir Crit Care Med. 154 (6), 1868-1902 (1996).","journal-title":"Am J Respir Crit Care Med"},{"issue":"1","key":"ref2","first-page":"81","type":"journal-article","article-title":"Type II alveolar cell. Defender of the alveolus","volume":"115","author":"Mason","year":"1977","unstructured":"Mason, R. J., Williams, M. C. Type II alveolar cell. Defender of the alveolus. Am Rev Respir Dis. 115 (1), 81-91 (1977).","journal-title":"Am Rev Respir Dis"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/rr36","type":"journal-article","article-title":"Alveolar epithelial type II cell: defender of the alveolus revisited","volume":"2","author":"Fehrenbach","year":"2001","unstructured":"Fehrenbach, H. Alveolar epithelial type II cell: defender of the alveolus revisited. Respir Res. 2 (1), 33-46 (2001).","journal-title":"Respir Res"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.stem.2024.02.009","type":"journal-article","article-title":"Lung repair and regeneration: Advanced models and insights into human disease","volume":"31","author":"Basil","year":"2024","unstructured":"Basil, M. C., Alysandratos, K.-D., Kotton, D. N., Morrisey, E. E. Lung repair and regeneration: Advanced models and insights into human disease. Cell Stem Cell. 31 (4), 439-454 (2024).","journal-title":"Cell Stem Cell"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1038\/nri3600","type":"journal-article","article-title":"Alveolar macrophages: plasticity in a tissue-specific context","volume":"14","author":"Hussell","year":"2014","unstructured":"Hussell, T., Bell, T. J. Alveolar macrophages: plasticity in a tissue-specific context. Nat Rev Immunol. 14 (2), 81-93 (2014).","journal-title":"Nat Rev Immunol"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"e20210745","DOI":"10.1084\/jem.20210745","type":"journal-article","article-title":"Alveolar macrophages rely on GM-CSF from alveolar epithelial type 2 cells before and after birth","volume":"218","author":"Gschwend","year":"2021","unstructured":"Gschwend, J. et al. Alveolar macrophages rely on GM-CSF from alveolar epithelial type 2 cells before and after birth. J Exp Med. 218 (1), e20210745 (2021).","journal-title":"J Exp Med"},{"issue":"7831","key":"ref7","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/s41586-020-2822-7","type":"journal-article","article-title":"Capillary cell-type specialization in the alveolus","volume":"586","author":"Gillich","year":"2020","unstructured":"Gillich, A. et al. Capillary cell-type specialization in the alveolus. Nature. 586 (7831), 785-789 (2020).","journal-title":"Nature"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"3964","DOI":"10.1038\/s41467-019-11843-0","type":"journal-article","article-title":"Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung","volume":"10","author":"Schyns","year":"2019","unstructured":"Schyns, J. et al. Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung. Nat Commun. 10 (1), 3964 (2019).","journal-title":"Nat Commun"},{"issue":"6432","key":"ref9","doi-asserted-by":"crossref","first-page":"eaat3773","DOI":"10.1126\/science.aau0964","type":"journal-article","article-title":"Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches","volume":"363","author":"Chakarov","year":"2019","unstructured":"Chakarov, S. et al. Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches. Science. 363 (6432), eaat3773 (2019).","journal-title":"Science"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"1920","DOI":"10.1038\/s41467-020-15647-5","type":"journal-article","article-title":"Collagen-producing lung cell atlas identifies multiple subsets with distinct localization and relevance to fibrosis","volume":"11","author":"Tsukui","year":"1920","unstructured":"Tsukui, T. et al. Collagen-producing lung cell atlas identifies multiple subsets with distinct localization and relevance to fibrosis. Nat Commun. 11 (1), 1920 (2020).","journal-title":"Nat Commun"},{"issue":"2","key":"ref11","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1038\/s41590-019-0568-x","type":"journal-article","article-title":"Influenza-induced monocyte-derived alveolar macrophages confer prolonged antibacterial protection","volume":"21","author":"Aegerter","year":"2020","unstructured":"Aegerter, H. et al. Influenza-induced monocyte-derived alveolar macrophages confer prolonged antibacterial protection. Nat Immunol. 21 (2), 145-157 (2020).","journal-title":"Nat Immunol"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1038\/s41385-021-00397-4","type":"journal-article","article-title":"Neutrophils in respiratory viral infections","volume":"14","author":"Johansson","year":"2021","unstructured":"Johansson, C., Kirsebom, F. C. M. Neutrophils in respiratory viral infections. Mucosal Immunol. 14 (4), 815-827 (2021).","journal-title":"Mucosal Immunol"},{"issue":"71","key":"ref13","doi-asserted-by":"crossref","first-page":"eabj5761","DOI":"10.1126\/sciimmunol.abj5761","type":"journal-article","article-title":"Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection","volume":"7","author":"Li","year":"2022","unstructured":"Li, F. et al. Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection. Sci Immunol. 7 (71), eabj5761 (2022).","journal-title":"Sci Immunol"},{"issue":"143","key":"ref14","first-page":"e58101","type":"journal-article","article-title":"Orthotopic transplantation of syngeneic lung adenocarcinoma cells to study PD-L1 expression","author":"Moll","year":"2019","unstructured":"Moll, H. P. et al. Orthotopic transplantation of syngeneic lung adenocarcinoma cells to study PD-L1 expression. J Vis Exp. 143, e58101 (2019).","journal-title":"J Vis Exp"},{"issue":"1","key":"ref15","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1006\/exmp.2001.2376","type":"journal-article","article-title":"TNF-\u03b1, PDGF, TGF-\u03b21 expression by primary mouse bronchiolar-alveolar epithelial and mesenchymal cells: TNF-\u03b1 induces TGF-\u03b21","volume":"71","author":"Warshamana","year":"2001","unstructured":"Warshamana, G. S., Corti, M., Brody, A. R. TNF-\u03b1, PDGF, and TGF-\u03b21 expression by primary mouse bronchiolar-alveolar epithelial and mesenchymal cells: TNF-\u03b1 induces TGF-\u03b21. Exp Mol Pathol. 71 (1), 13-33 (2001).","journal-title":"Exp Mol Pathol"},{"issue":"3","key":"ref16","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1165\/ajrcmb.14.4.8600933","type":"journal-article","article-title":"Isolation and primary culture of murine alveolar type II cells","volume":"14","author":"Corti","year":"1996","unstructured":"Corti, M., Brody, A. R., Harrison, J. H. Isolation and primary culture of murine alveolar type II cells. Am J Respir Cell Mol Biol. 14 (3), 309-315 (1996).","journal-title":"Am J Respir Cell Mol Biol"},{"issue":"5","key":"ref17","doi-asserted-by":"crossref","first-page":"e1005544","DOI":"10.1371\/journal.ppat.1005544","type":"journal-article","article-title":"Influenza virus infects epithelial stem\/progenitor cells of the distal lung: Impact on Fgfr2b-driven epithelial repair","volume":"12","author":"Quantius","year":"2016","unstructured":"Quantius, J. et al. Influenza virus infects epithelial stem\/progenitor cells of the distal lung: Impact on Fgfr2b-driven epithelial repair. PLoS Pathog. 12 (5), e1005544 (2016).","journal-title":"PLoS Pathog"},{"issue":"8","key":"ref18","first-page":"e3988","type":"journal-article","article-title":"Transcardiac perfusion of the mouse for brain tissue dissection and fixation","volume":"11","author":"Wu","year":"2021","unstructured":"Wu, J. et al. Transcardiac perfusion of the mouse for brain tissue dissection and fixation. BIO Protoc. 11 (8), e3988 (2021).","journal-title":"BIO Protoc"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"1313676","DOI":"10.1080\/20010001.2017.1313676","type":"journal-article","article-title":"Guidelines for collection and processing of lungs from aged mice for histological studies","volume":"7","author":"Morton","year":"2017","unstructured":"Morton, J., Snider, T. A. Guidelines for collection and processing of lungs from aged mice for histological studies. Pathobiol Aging Age Relat Dis. 7 (1), 1313676 (2017).","journal-title":"Pathobiol Aging Age Relat Dis"},{"issue":"108","key":"ref20","first-page":"e53741","type":"journal-article","article-title":"Ex vivo live imaging of lung metastasis and their microenvironment","author":"Bijgaart","year":"2016","unstructured":"Bijgaart, R. J. E. van den, Kong, N., Maynard, C., Plaks, V. Ex vivo live imaging of lung metastasis and their microenvironment. J Vis Exp. 108, e53741 (2016).","journal-title":"J Vis Exp"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"106632","DOI":"10.1016\/j.intimp.2020.106632","type":"journal-article","article-title":"Isolation and purification of immune cells from the liver","volume":"85","author":"Shi","year":"2020","unstructured":"Shi, W. et al. Isolation and purification of immune cells from the liver. Int Immunopharmacol. 85, 106632 (2020).","journal-title":"Int Immunopharmacol"},{"issue":"3","key":"ref22","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1183\/13993003.01282-2015","type":"journal-article","article-title":"Influenza virus damages the alveolar barrier by disrupting epithelial cell tight junctions","volume":"47","author":"Short","year":"2016","unstructured":"Short, K. R. et al. Influenza virus damages the alveolar barrier by disrupting epithelial cell tight junctions. Eur Respir J. 47 (3), 954-966 (2016).","journal-title":"Eur Respir J"},{"issue":"4","key":"ref23","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.immuni.2014.09.007","type":"journal-article","article-title":"CD4+ T cell help guides formation of CD103+ lung-resident memory CD8+ T cells during influenza viral infection","volume":"41","author":"Laidlaw","year":"2014","unstructured":"Laidlaw, B. J. et al. CD4+ T cell help guides formation of CD103+ lung-resident memory CD8+ T cells during influenza viral infection. Immunity. 41 (4), 633-645 (2014).","journal-title":"Immunity"},{"issue":"2","key":"ref24","doi-asserted-by":"crossref","first-page":"101652","DOI":"10.1016\/j.xpro.2022.101652","type":"journal-article","article-title":"Protocol for isolation and characterization of lung tissue-resident memory T cells and airway-trained innate immunity after intranasal vaccination in mice","volume":"3","author":"D'Agostino","year":"2022","unstructured":"D'Agostino, M. R. et al. Protocol for isolation and characterization of lung tissue-resident memory T cells and airway-trained innate immunity after intranasal vaccination in mice. STAR Protoc. 3 (2), 101652 (2022).","journal-title":"STAR Protoc"},{"issue":"4","key":"ref25","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1136\/thoraxjnl-2021-217997","type":"journal-article","article-title":"Lung epithelial cells interact with immune cells and bacteria to shape the microenvironment in tuberculosis","volume":"77","author":"Waal","year":"2022","unstructured":"Waal, A. M. de, Hiemstra, P. S., Ottenhoff, T. H., Joosten, S. A., Does, A. M. van der. Lung epithelial cells interact with immune cells and bacteria to shape the microenvironment in tuberculosis. Thorax. 77 (4), 408-416 (2022).","journal-title":"Thorax"},{"issue":"4","key":"ref26","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1007\/s00281-016-0569-x","type":"journal-article","article-title":"Macrophage-epithelial interactions in pulmonary alveoli","volume":"38","author":"Bhattacharya","year":"2016","unstructured":"Bhattacharya, J., Westphalen, K. Macrophage-epithelial interactions in pulmonary alveoli. Semin Immunopathol. 38 (4), 461-469 (2016).","journal-title":"Semin Immunopathol"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/67648\/isolation-live-myeloid-epithelial-cell-populations-from-mouse","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:42:57Z","timestamp":1792597377000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/67648\/isolation-live-myeloid-epithelial-cell-populations-from-mouse"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,31]]},"references-count":26,"journal-issue":{"issue":"215","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/67648","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,31]]}}}