{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:40:49Z","timestamp":1792597249775,"version":"4.5.0"},"reference-count":32,"publisher":"MyJove Corporation","issue":"221","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/67701","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T18:03:30Z","timestamp":1753985010000},"source":"Crossref","is-referenced-by-count":0,"title":["Glucose-Stimulated Insulin Secretion via Perfusion through the Mice Vasculature with an Intact Pancreas"],"prefix":"10.3791","author":[{"given":"Jerice","family":"Banola","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon C.","family":"Weir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Aguayo-Mazzucato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/S0039-6060(99)70183-2","type":"journal-article","article-title":"Structural and functional changes resulting from islet isolation lead to islet cell death","volume":"126","author":"Rosenberg","year":"1999","unstructured":"Rosenberg, L., Wang, R., Paraskevas, S. Structural and functional changes resulting from islet isolation lead to islet cell death. Surgery. 126 (2), 393-398 (1999).","journal-title":"Surgery"},{"issue":"1-2","key":"ref2","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/S0041-1345(96)00452-6","type":"journal-article","article-title":"Apoptosis occurs in freshly isolated human islets under standard culture conditions","volume":"29","author":"Paraskevas","year":"1997","unstructured":"Paraskevas, S. et al. Apoptosis occurs in freshly isolated human islets under standard culture conditions. Transplant Proc. 29 (1-2), 750-752 (1997).","journal-title":"Transplant Proc"},{"issue":"10","key":"ref3","doi-asserted-by":"crossref","first-page":"2559","DOI":"10.2337\/diabetes.53.10.2559","type":"journal-article","article-title":"Response of human islets to isolation stress and the effect of antioxidant treatment","volume":"53","author":"Bottino","year":"2004","unstructured":"Bottino, R. et al. Response of human islets to isolation stress and the effect of antioxidant treatment. Diabetes. 53 (10), 2559-2568 (2004).","journal-title":"Diabetes"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"667170","DOI":"10.3389\/fendo.2021.667170","type":"journal-article","article-title":"The role of vascular cells in pancreatic beta-cell function","volume":"12","author":"Burganova","year":"2021","unstructured":"Burganova, G., Bridges, C., Thorn, P., Landsman, L. The role of vascular cells in pancreatic beta-cell function. Front Endocrinol. 12, 667170 (2021).","journal-title":"Front Endocrinol"},{"issue":"6","key":"ref5","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1007\/s00125-002-0827-4","type":"journal-article","article-title":"Graft vascular function after transplantation of pancreatic islets","volume":"45","author":"Jansson","year":"2002","unstructured":"Jansson, L., Carlsson, P. -O. Graft vascular function after transplantation of pancreatic islets. Diabetologia. 45 (6), 749-763 (2002).","journal-title":"Diabetologia"},{"issue":"9","key":"ref6","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1016\/j.molmet.2017.03.014","type":"journal-article","article-title":"Intraocular in vivo imaging of pancreatic islet cell physiology\/pathology","volume":"6","author":"Leibiger","year":"2017","unstructured":"Leibiger, I. B., Berggren, P. -O. Intraocular in vivo imaging of pancreatic islet cell physiology\/pathology. Mol Metab. 6 (9), 1002-1009 (2017).","journal-title":"Mol Metab"},{"issue":"11 Suppl","key":"ref7","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1016\/S0026-0495(76)80183-7","type":"journal-article","article-title":"Glucagon secretion from the perfused rat pancreas following acute and chronic streptozotocin","volume":"25","author":"Weir","year":"1976","unstructured":"Weir, G. C., Atkins, R. F., Martin, D. B. Glucagon secretion from the perfused rat pancreas following acute and chronic streptozotocin. Metabolism. 25 (11 Suppl), 1519-1521 (1976).","journal-title":"Metabolism"},{"issue":"11","key":"ref8","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1038\/labinvest.2010.124","type":"journal-article","article-title":"Quantitative analysis of cell composition and purity of human pancreatic islet preparations","volume":"90","author":"Pisania","year":"2010","unstructured":"Pisania, A. et al. Quantitative analysis of cell composition and purity of human pancreatic islet preparations. Lab Invest. 90 (11), 1661-1675 (2010).","journal-title":"Lab Invest"},{"issue":"1","key":"ref9","first-page":"62","type":"journal-article","article-title":"Characteristics of insulin and glucagon release from the perfused pancreas, intact isolated islets, and dispersed islet cells","volume":"24","author":"Weir","year":"1986","unstructured":"Weir, G. C., Leahy, J. L., Braunstein, L. P. Characteristics of insulin and glucagon release from the perfused pancreas, intact isolated islets, and dispersed islet cells. Horm Res. 24 (1), 62-72 (1986).","journal-title":"Horm Res"},{"key":"ref10","type":"book","article-title":"Collagenases in Pancreatic Islet Isolation","volume-title":"Transplantation, Bioengineering, and Regeneration of the Endocrine Pancreas","author":"Fathi","year":"2019","unstructured":"Fathi, I., Goto, M. Collagenases in Pancreatic Islet Isolation. Transplantation, Bioengineering, and Regeneration of the Endocrine Pancreas. Elsevier Academic Press (2019)."},{"issue":"6","key":"ref11","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1002\/jcla.1860080611","type":"journal-article","article-title":"Isolation of porcine pancreatic islets: low trypsin activity during the isolation procedure guarantees reproducible high islet yields","volume":"8","author":"Heiser","year":"1994","unstructured":"Heiser, A., Ulrichs, K., M\u00fcller-Ruchholtz, W. Isolation of porcine pancreatic islets: low trypsin activity during the isolation procedure guarantees reproducible high islet yields. J Clin Lab Anal. 8 (6), 407-411 (1994).","journal-title":"J Clin Lab Anal"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1016\/S0041-1345(97)00168-1","type":"journal-article","article-title":"Improved outcome of pig islet isolation by Pefabloc inhibition of trypsin","volume":"29","author":"Basir","year":"1997","unstructured":"Basir, I. et al. Improved outcome of pig islet isolation by Pefabloc inhibition of trypsin. Transplant Proc. 29 (4), 1939-1941 (1997).","journal-title":"Transplant Proc"},{"issue":"3","key":"ref13","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1177\/096368979900800307","type":"journal-article","article-title":"A preliminary study of the activation of endogenous pancreatic exocrine enzymes during automated porcine islet isolation","volume":"8","author":"White","year":"1999","unstructured":"White, S. A. et al. A preliminary study of the activation of endogenous pancreatic exocrine enzymes during automated porcine islet isolation. Cell Transplant. 8 (3), 265-276 (1999).","journal-title":"Cell Transplant"},{"issue":"4","key":"ref14","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1097\/00007890-200108270-00003","type":"journal-article","article-title":"Serine-protease inhibition during islet isolation increases islet yield from human pancreases with prolonged ischemia","volume":"72","author":"Lakey","year":"2001","unstructured":"Lakey, J. R. T. et al. Serine-protease inhibition during islet isolation increases islet yield from human pancreases with prolonged ischemia. Transplantation. 72 (4), 565-570 (2001).","journal-title":"Transplantation"},{"issue":"4","key":"ref15","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1097\/01.TP.0000046537.47139.CE","type":"journal-article","article-title":"Evaluation of Pefabloc as a serine protease inhibitor during human-islet isolation","volume":"75","author":"Rose","year":"2003","unstructured":"Rose, N. L., Palcic, M. M., Helms, L. M. H., Lakey, J. R. T. Evaluation of Pefabloc as a serine protease inhibitor during human-islet isolation. Transplantation. 75 (4), 462-466 (2003).","journal-title":"Transplantation"},{"issue":"7","key":"ref16","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1097\/TP.0b013e318186df87","type":"journal-article","article-title":"Collagenase penetrates human pancreatic islets following standard intraductal administration","volume":"86","author":"Cross","year":"2008","unstructured":"Cross, S. E. et al. Collagenase penetrates human pancreatic islets following standard intraductal administration. Transplantation. 86 (7), 907-911 (2008).","journal-title":"Transplantation"},{"issue":"11","key":"ref17","doi-asserted-by":"crossref","first-page":"2671","DOI":"10.1111\/j.1600-6143.2005.01078.x","type":"journal-article","article-title":"Harmful delayed effects of exogenous isolation enzymes on isolated human islets: relevance to clinical transplantation","volume":"5","author":"Balamurugan","year":"2005","unstructured":"Balamurugan, A. N. et al. Harmful delayed effects of exogenous isolation enzymes on isolated human islets: relevance to clinical transplantation. Am J Transplant. 5 (11), 2671-2681 (2005).","journal-title":"Am J Transplant"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.semcdb.2020.02.002","type":"journal-article","article-title":"Functional analysis of islet cells in vitro, in situ, and in vivo","volume":"103","author":"Li","year":"2020","unstructured":"Li, W. Functional analysis of islet cells in vitro, in situ, and in vivo. Semin Cell Dev Biol. 103, 14-19 (2020).","journal-title":"Semin Cell Dev Biol"},{"issue":"12","key":"ref19","doi-asserted-by":"crossref","first-page":"2809","DOI":"10.1038\/nprot.2014.195","type":"journal-article","article-title":"Using pancreas tissue slices for in situ studies of islet of Langerhans and acinar cell biology","volume":"9","author":"Marciniak","year":"2014","unstructured":"Marciniak, A. et al. Using pancreas tissue slices for in situ studies of islet of Langerhans and acinar cell biology. Nat Protoc. 9 (12), 2809-2822 (2014).","journal-title":"Nat Protoc"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"11","DOI":"10.2337\/dbi20-0018","type":"journal-article","article-title":"Bridging the gap: pancreas tissue slices from organ and tissue donors for the study of diabetes pathogenesis","volume":"73","author":"Cohrs","year":"2024","unstructured":"Cohrs, C. M. et al. Bridging the gap: pancreas tissue slices from organ and tissue donors for the study of diabetes pathogenesis. Diabetes. 73 (1), 11-22 (2024).","journal-title":"Diabetes"},{"issue":"8","key":"ref21","doi-asserted-by":"crossref","first-page":"e134525","DOI":"10.1172\/jci.insight.134525","type":"journal-article","article-title":"Pancreas tissue slices from organ donors enable in situ analysis of type 1 diabetes pathogenesis","volume":"5","author":"Panzer","year":"2020","unstructured":"Panzer, J. K. et al. Pancreas tissue slices from organ donors enable in situ analysis of type 1 diabetes pathogenesis. JCI Insight. 5 (8), e134525 (2020).","journal-title":"JCI Insight"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/978-1-4939-9882-1_17","type":"journal-article","article-title":"Measurement of insulin secretion using pancreas perfusion in the rodent","volume":"2076","author":"Wargent","year":"2020","unstructured":"Wargent, E. T. Measurement of insulin secretion using pancreas perfusion in the rodent. Methods Mol Biol. 2076, 281-297 (2020).","journal-title":"Methods Mol Biol"},{"issue":"8","key":"ref23","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.2337\/db21-1104","type":"journal-article","article-title":"Pericyte control of blood flow in intraocular islet grafts impacts glucose homeostasis in mice","volume":"71","author":"Tamayo","year":"2022","unstructured":"Tamayo, A. et al. Pericyte control of blood flow in intraocular islet grafts impacts glucose homeostasis in mice. Diabetes. 71 (8), 1679-1693 (2022).","journal-title":"Diabetes"},{"issue":"11","key":"ref24","first-page":"931","type":"journal-article","article-title":"Ang(1-7) treatment attenuates \u03b2-cell dysfunction by improving pancreatic microcirculation in a rat model of type 2 diabetes","volume":"36","author":"Yuan","year":"2013","unstructured":"Yuan, L., Li, Y., Li, G., Song, Y., Gong, X. Ang(1-7) treatment attenuates \u03b2-cell dysfunction by improving pancreatic microcirculation in a rat model of type 2 diabetes. J Endocrinol Invest. 36 (11), 931-937 (2013).","journal-title":"J Endocrinol Invest"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s001250050880","type":"journal-article","article-title":"Angiotensin II and the endocrine pancreas: effects on islet blood flow and insulin secretion in rats","volume":"41","author":"Carlsson","year":"1998","unstructured":"Carlsson, P. -O., Berne, C., Jansson, L. Angiotensin II and the endocrine pancreas: effects on islet blood flow and insulin secretion in rats. Diabetologia. 41 (2), 127-133 (1998).","journal-title":"Diabetologia"},{"issue":"11","key":"ref26","doi-asserted-by":"crossref","first-page":"733","DOI":"10.2337\/diab.18.11.733","type":"journal-article","article-title":"A rat pancreas-small gut preparation for the study of intestinal factor(s) and insulin release","volume":"18","author":"Penhos","year":"1969","unstructured":"Penhos, J. C. et al. A rat pancreas-small gut preparation for the study of intestinal factor(s) and insulin release. Diabetes. 18 (11), 733-738 (1969).","journal-title":"Diabetes"},{"issue":"3","key":"ref27","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1530\/eje.0.1320370","type":"journal-article","article-title":"Differences in insulin secretion between the rat and mouse: role of cAMP","volume":"132","author":"Ma","year":"1995","unstructured":"Ma, Y. H. et al. Differences in insulin secretion between the rat and mouse: role of cAMP. Eur J Endocrinol. 132 (3), 370-376 (1995).","journal-title":"Eur J Endocrinol"},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1007\/BF01221854","type":"journal-article","article-title":"Anaesthesia and insulin secretion: the effects of diethyl ether, halothane, pentobarbitone sodium and ketamine hydrochloride on intravenous glucose tolerance and insulin secretion in the rat","volume":"9","author":"Aynsley-Green","year":"1973","unstructured":"Aynsley-Green, A., Biebuyck, J. F., Alberti, K. G. Anaesthesia and insulin secretion: the effects of diethyl ether, halothane, pentobarbitone sodium and ketamine hydrochloride on intravenous glucose tolerance and insulin secretion in the rat. Diabetologia. 9 (4), 274-281 (1973).","journal-title":"Diabetologia"},{"issue":"7","key":"ref29","first-page":"11347","type":"journal-article","article-title":"Diabetes alters the blood glucose response to ketamine in streptozotocin-diabetic rats","volume":"8","author":"Chen","year":"2015","unstructured":"Chen, H., Li, L., Xia, H. Diabetes alters the blood glucose response to ketamine in streptozotocin-diabetic rats. Int J Clin Exp Med. 8 (7), 11347-11351 (2015).","journal-title":"Int J Clin Exp Med"},{"issue":"6","key":"ref30","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1111\/j.1600-0684.1997.tb00060.x","type":"journal-article","article-title":"Effects of ketamine sedation on glucose clearance, insulin secretion and counterregulatory hormone production in baboons (Papio hamadryas)","volume":"26","author":"Lehmann","year":"1997","unstructured":"Lehmann, R. et al. Effects of ketamine sedation on glucose clearance, insulin secretion and counterregulatory hormone production in baboons (Papio hamadryas). J Med Primatol. 26 (6), 312-321 (1997).","journal-title":"J Med Primatol"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","first-page":"38","DOI":"10.3844\/ajptsp.2009.38.45","type":"journal-article","article-title":"Effect of intravenous ketamine administration on blood glucose levels in conscious rabbits","volume":"4","author":"Sharif","year":"2009","unstructured":"Sharif, S. I., Abouazra, H. A. Effect of intravenous ketamine administration on blood glucose levels in conscious rabbits. Am J Pharm Toxicol. 4 (2), 38-45 (2009).","journal-title":"Am J Pharm Toxicol"},{"issue":"6","key":"ref32","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1111\/jdi.12094","type":"journal-article","article-title":"Glucose-stimulated insulin secretion: a newer perspective","volume":"4","author":"Komatsu","year":"2013","unstructured":"Komatsu, M., Takei, M., Ishii, H., Sato, Y. Glucose-stimulated insulin secretion: a newer perspective. J Diabetes Investig. 4 (6), 511-516 (2013).","journal-title":"J Diabetes Investig"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/67701\/glucose-stimulated-insulin-secretion-via-perfusion-through-mice","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:44:33Z","timestamp":1792593873000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/67701\/glucose-stimulated-insulin-secretion-via-perfusion-through-mice"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,25]]},"references-count":32,"journal-issue":{"issue":"221","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/67701","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,25]]}}}