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CRISPR-Cas9 system as a versatile tool for genome engineering in human cells. Mol Ther Nucleic Acids. 5 (11), e388 (2016).","journal-title":"Mol Ther Nucleic Acids"},{"issue":"11","key":"ref53","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.2337\/db23-0445","type":"journal-article","article-title":"Establishment of pancreatic -cell-specific gene knock-out system based on CRISPR-Cas9 technology with AAV8-mediated gRNA delivery","volume":"72","author":"Ueki","year":"2023","unstructured":"Ueki, K. et al. Establishment of pancreatic -cell-specific gene knock-out system based on CRISPR-Cas9 technology with AAV8-mediated gRNA delivery. Diabetes. 72 (11), 1609-1620 (2023).","journal-title":"Diabetes"},{"issue":"17-18","key":"ref54","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1089\/hum.2019.284","type":"journal-article","article-title":"Single-use capture purification of adeno-associated viral gene transfer vectors by membrane-based steric exclusion chromatography","volume":"32","author":"Marichal-Gallardo","year":"2021","unstructured":"Marichal-Gallardo, P. et al. Single-use capture purification of adeno-associated viral gene transfer vectors by membrane-based steric exclusion chromatography. Hum Gene Ther. 32 (17-18), 959-974 (2021).","journal-title":"Hum Gene Ther"},{"issue":"2","key":"ref55","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.jviromet.2012.04.004","type":"journal-article","article-title":"Rapid and simplified purification of recombinant adeno-associated virus","volume":"183","author":"Guo","year":"2012","unstructured":"Guo, P. et al. Rapid and simplified purification of recombinant adeno-associated virus. J Virol Methods. 183 (2), 139-146 (2012).","journal-title":"J Virol Methods"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"113863","DOI":"10.1016\/j.jviromet.2020.113863","type":"journal-article","article-title":"A simple, two-step, small-scale purification of recombinant adeno-associated viruses","volume":"281","author":"Chen","year":"2020","unstructured":"Chen, S. H. et al. A simple, two-step, small-scale purification of recombinant adeno-associated viruses. 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