{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:57:09Z","timestamp":1792598229817,"version":"4.5.0"},"reference-count":40,"publisher":"MyJove Corporation","issue":"217","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/67732","type":"journal-article","created":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T19:07:15Z","timestamp":1742584035000},"source":"Crossref","is-referenced-by-count":3,"title":["Genome Editing in the Yellow Fever Mosquito <em>Aedes aegypti<\/em> using CRISPR-Cas9"],"prefix":"10.3791","author":[{"given":"Iliano V.","family":"Coutinho-Abreu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsing-Han","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noah H.","family":"Rose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar S.","family":"Akbari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,3,21]]},"reference":[{"key":"ref1","type":"book","article-title":"Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors","volume-title":"Nat Biotechnol","author":"Anzalone","year":"2020","unstructured":"Anzalone, A. V., Koblan, L. W., Liu, D. R. Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors. Nat Biotechnol. 38 (7), 824-844 (2020)."},{"issue":"7320","key":"ref2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/nature09523","type":"journal-article","article-title":"The CRISPR\/Cas bacterial immune system cleaves bacteriophage and plasmid DNA","volume":"468","author":"Garneau","year":"2010","unstructured":"Garneau, J. E. et al. The CRISPR\/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature. 468 (7320), 67-71 (2010).","journal-title":"Nature"},{"issue":"6096","key":"ref3","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1126\/science.1225829","type":"journal-article","article-title":"A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity","volume":"337","author":"Jinek","year":"2012","unstructured":"Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 337 (6096), 816-821 (2012).","journal-title":"Science"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"322","DOI":"10.3390\/genes13020322","type":"journal-article","article-title":"DNA double-strand break repairs and their application in plant DNA integration","volume":"13","author":"Shen","year":"2022","unstructured":"Shen, H., Li, Z. DNA double-strand break repairs and their application in plant DNA integration. Genes (Basel). 13 (2), 322 (2022).","journal-title":"Genes (Basel)"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.cub.2017.12.015","type":"journal-article","article-title":"Modulation of host learning in Aedes aegypti mosquitoes","volume":"28","author":"Vinauger","year":"2018","unstructured":"Vinauger, C. et al. Modulation of host learning in Aedes aegypti mosquitoes. Curr Biol. 28 (3), 333-344.e8 (2018).","journal-title":"Curr Biol"},{"issue":"49","key":"ref6","doi-asserted-by":"crossref","first-page":"E10540","DOI":"10.1073\/pnas.1711538114","type":"journal-article","article-title":"Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector","volume":"114","author":"Li","year":"2017","unstructured":"Li, M., Bui, M., Yang, T., Bowman, C. S., White, B. J., Akbari, O. S. Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector. Proc Natl Acad Sci U S A. 114 (49), E10540-E10549 (2017).","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"3008","DOI":"10.1038\/s41467-018-05425-9","type":"journal-article","article-title":"Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing","volume":"9","author":"Chaverra-Rodriguez","year":"2018","unstructured":"Chaverra-Rodriguez, D. et al. Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing. Nat Commun. 9 (1), 3008 (2018).","journal-title":"Nat Commun"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1381164","DOI":"10.3389\/fphys.2024.1381164","type":"journal-article","article-title":"Transgenic line for characterizing GABA-receptor expression to study the neural basis of olfaction in the yellow-fever mosquito","volume":"15","author":"Rouyar","year":"2024","unstructured":"Rouyar, A. et al. Transgenic line for characterizing GABA-receptor expression to study the neural basis of olfaction in the yellow-fever mosquito. Front Physiol. 15, 1381164 (2024).","journal-title":"Front Physiol"},{"issue":"2","key":"ref9","doi-asserted-by":"crossref","first-page":"e1010060","DOI":"10.1371\/journal.pgen.1010060","type":"journal-article","article-title":"Considerations for homology-based DNA repair in mosquitoes: Impact of sequence heterology and donor template source","volume":"18","author":"Ang","year":"2022","unstructured":"Ang, J. X. D. et al. Considerations for homology-based DNA repair in mosquitoes: Impact of sequence heterology and donor template source. PLoS Genet. 18 (2), e1010060 (2022).","journal-title":"PLoS Genet"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s13059-017-1164-8","type":"journal-article","article-title":"Efficient precise knockin with a double cut HDR donor after CRISPR\/Cas9-mediated double-stranded DNA cleavage","volume":"18","author":"Zhang","year":"2017","unstructured":"Zhang, J.-P. et al. Efficient precise knockin with a double cut HDR donor after CRISPR\/Cas9-mediated double-stranded DNA cleavage. Genome Biol. 18 (1), 35 (2017).","journal-title":"Genome Biol"},{"issue":"2","key":"ref11","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.tig.2022.10.007","type":"journal-article","article-title":"Technological advances in mosquito olfaction neurogenetics","volume":"39","author":"Coutinho-Abreu","year":"2023","unstructured":"Coutinho-Abreu, I. V., Akbari, O. S. Technological advances in mosquito olfaction neurogenetics. Trends Genet. 39 (2), 154-166 (2023).","journal-title":"Trends Genet"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"RP90199","DOI":"10.7554\/eLife.90199.3","type":"journal-article","article-title":"Targeting sex determination to suppress mosquito populations","volume":"12","author":"Li","year":"2024","unstructured":"Li, M. et al. Targeting sex determination to suppress mosquito populations. eLife. 12, RP90199 (2024).","journal-title":"eLife"},{"issue":"18","key":"ref13","doi-asserted-by":"crossref","first-page":"4180","DOI":"10.1016\/j.cub.2021.07.003","type":"journal-article","article-title":"Elimination of vision-guided target attraction in Aedes aegypti using CRISPR","volume":"31","author":"Zhan","year":"2021","unstructured":"Zhan, Y., Alonso San Alberto, D., Rusch, C., Riffell, J. A., Montell, C. Elimination of vision-guided target attraction in Aedes aegypti using CRISPR. Current Biol. 31 (18), 4180-4187.e6 (2021).","journal-title":"Current Biol"},{"issue":"6478","key":"ref14","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1126\/science.aay9847","type":"journal-article","article-title":"Mosquito heat seeking is driven by an ancestral cooling receptor","volume":"367","author":"Greppi","year":"2020","unstructured":"Greppi, C. et al. Mosquito heat seeking is driven by an ancestral cooling receptor. Science. 367 (6478), 681-684 (2020).","journal-title":"Science"},{"issue":"3","key":"ref15","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.cell.2010.02.025","type":"journal-article","article-title":"The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis","volume":"141","author":"Potter","year":"2010","unstructured":"Potter, C. J., Tasic, B., Russler, E. V., Liang, L., Luo, L. The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis. Cell. 141 (3), 536-548 (2010).","journal-title":"Cell"},{"issue":"3","key":"ref16","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1038\/nmeth.3250","type":"journal-article","article-title":"Improved and expanded Q-system reagents for genetic manipulations","volume":"12","author":"Riabinina","year":"2015","unstructured":"Riabinina, O. et al. Improved and expanded Q-system reagents for genetic manipulations. Nat Methods. 12 (3), 219-222, 5 p following 222 (2015).","journal-title":"Nat Methods"},{"issue":"W1","key":"ref17","doi-asserted-by":"crossref","first-page":"W171","DOI":"10.1093\/nar\/gkz365","type":"journal-article","article-title":"CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing","volume":"47","author":"Labun","year":"2019","unstructured":"Labun, K. et al. CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing. Nucleic Acids Res. 47 (W1), W171-W174 (2019).","journal-title":"Nucleic Acids Res"},{"issue":"W1","key":"ref18","doi-asserted-by":"crossref","first-page":"W242","DOI":"10.1093\/nar\/gky354","type":"journal-article","article-title":"CRISPOR: intuitive guide selection for CRISPR\/Cas9 genome editing experiments and screens","volume":"46","author":"Concordet","year":"2018","unstructured":"Concordet, J.-P., Haeussler, M. CRISPOR: intuitive guide selection for CRISPR\/Cas9 genome editing experiments and screens. Nucleic Acids Res. 46 (W1), W242-W245 (2018).","journal-title":"Nucleic Acids Res"},{"key":"ref19","first-page":"e65136","type":"journal-article","article-title":"Quantifying fitness costs in transgenic Aedes aegypti mosquitoes","author":"Williams","year":"2023","unstructured":"Williams, A. E. et al. Quantifying fitness costs in transgenic Aedes aegypti mosquitoes. J Vis Exp. (199), doi: 10.3791\/65136 (2023).","journal-title":"J Vis Exp"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.celrep.2013.06.020","type":"journal-article","article-title":"Highly efficient targeted mutagenesis of Drosophila with the CRISPR\/Cas9 system","volume":"4","author":"Bassett","year":"2013","unstructured":"Bassett, A. R., Tibbit, C., Ponting, C. P., Liu, J.-L. Highly efficient targeted mutagenesis of Drosophila with the CRISPR\/Cas9 system. Cell Rep. 4 (1), 220-228 (2013).","journal-title":"Cell Rep"},{"issue":"1","key":"ref21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.parint.2009.10.002","type":"journal-article","article-title":"Transgenesis and paratransgenesis to control insect-borne diseases: current status and future challenges","volume":"59","author":"Coutinho-Abreu","year":"2010","unstructured":"Coutinho-Abreu, I. V., Zhu, K. Y., Ramalho-Ortigao, M. Transgenesis and paratransgenesis to control insect-borne diseases: current status and future challenges. Parasitol Int. 59 (1), 1-8 (2010).","journal-title":"Parasitol Int"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1534\/genetics.110.120717","type":"journal-article","article-title":"Targeting DNA double-strand breaks with TAL effector nucleases","volume":"186","author":"Christian","year":"2010","unstructured":"Christian, M. et al. Targeting DNA double-strand breaks with TAL effector nucleases. Genetics. 186 (2), 757-761 (2010).","journal-title":"Genetics"},{"issue":"3","key":"ref23","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1073\/pnas.93.3.1156","type":"journal-article","article-title":"Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain","volume":"93","author":"Kim","year":"1996","unstructured":"Kim, Y. G., Cha, J., Chandrasegaran, S. Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain. Proc Natl Acad Sci U S A. 93 (3), 1156-1160 (1996).","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7455","key":"ref24","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1038\/nature12206","type":"journal-article","article-title":"orco mutant mosquitoes lose strong preference for humans and are not repelled by volatile DEET","volume":"498","author":"DeGennaro","year":"2013","unstructured":"DeGennaro, M. et al. orco mutant mosquitoes lose strong preference for humans and are not repelled by volatile DEET. Nature. 498 (7455), 487-491 (2013).","journal-title":"Nature"},{"issue":"5","key":"ref25","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1016\/j.cell.2013.12.044","type":"journal-article","article-title":"Multimodal integration of carbon dioxide and other sensory cues drives mosquito attraction to humans","volume":"156","author":"McMeniman","year":"2014","unstructured":"McMeniman, C. J., Corfas, R. A., Matthews, B. J., Ritchie, S. A., Vosshall, L. B. Multimodal integration of carbon dioxide and other sensory cues drives mosquito attraction to humans. Cell. 156 (5), 1060-1071 (2014).","journal-title":"Cell"},{"issue":"3","key":"ref26","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1038\/nprot.2006.221","type":"journal-article","article-title":"High efficiency germ-line transformation of mosquitoes","volume":"1","author":"Lobo","year":"2006","unstructured":"Lobo, N. F., Clayton, J. R., Fraser, M. J., Kafatos, F. C., Collins, F. H. High efficiency germ-line transformation of mosquitoes. Nat Protoc. 1 (3), 1312-1317 (2006).","journal-title":"Nat Protoc"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.celrep.2015.03.009","type":"journal-article","article-title":"Genome engineering with CRISPR-Cas9 in the mosquito Aedes aegypti","volume":"11","author":"Kistler","year":"2015","unstructured":"Kistler, K. E., Vosshall, L. B., Matthews, B. J. Genome engineering with CRISPR-Cas9 in the mosquito Aedes aegypti. Cell Rep. 11 (1), 51-60 (2015).","journal-title":"Cell Rep"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/S0965-1748(00)00119-3","type":"journal-article","article-title":"2nd Transformation of the Caribbean fruit fly, Anastrephasuspensa, with a piggyBac vector marked with polyubiquitin-regulated GFP","volume":"31","author":"Handler","year":"2001","unstructured":"Handler, A. M., Harrell, R. A., 2nd Transformation of the Caribbean fruit fly, Anastrephasuspensa, with a piggyBac vector marked with polyubiquitin-regulated GFP. Insect Biochem Mol Biol. 31 (2), 199-205 (2001).","journal-title":"Insect Biochem Mol Biol"},{"key":"ref29","type":"book","volume-title":"2nd Mosquito embryo microinjection under halocarbon oil or in aqueous solution","author":"Harrell","year":"2024","unstructured":"Harrell, R. A., 2nd Mosquito embryo microinjection under halocarbon oil or in aqueous solution. Cold Spring Harb Protoc. 2024 (7), db.prot108203 (2024)."},{"issue":"9","key":"ref30","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1101\/pdb.prot108085","type":"journal-article","article-title":"Generating mutant strains with transgenic Cas9","volume":"2023","author":"Sun","year":"2023","unstructured":"Sun, R., Raban, R., Akbari, O. S. Generating mutant strains with transgenic Cas9. Cold Spring Harb Protoc. 2023 (9), 671-678 (2023).","journal-title":"Cold Spring Harb Protoc"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","first-page":"100714","DOI":"10.1016\/j.crmeth.2024.100714","type":"journal-article","article-title":"An expanded neurogenetic toolkit to decode olfaction in the African malaria mosquito Anopheles gambiae","volume":"4","author":"Giraldo","year":"2024","unstructured":"Giraldo, D. et al. An expanded neurogenetic toolkit to decode olfaction in the African malaria mosquito Anopheles gambiae. Cell Rep Methods. 4 (2), 100714 (2024).","journal-title":"Cell Rep Methods"},{"issue":"2","key":"ref32","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.molcel.2021.12.002","type":"journal-article","article-title":"The CRISPR-Cas toolbox and gene editing technologies","volume":"82","author":"Liu","year":"2022","unstructured":"Liu, G., Lin, Q., Jin, S., Gao, C. The CRISPR-Cas toolbox and gene editing technologies. Mol Cell. 82 (2), 333-347 (2022).","journal-title":"Mol Cell"},{"issue":"5","key":"ref33","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nbt.3198","type":"journal-article","article-title":"Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells","volume":"33","author":"Chu","year":"2015","unstructured":"Chu, V. T. et al. Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 33 (5), 543-548 (2015).","journal-title":"Nat Biotechnol"},{"issue":"6","key":"ref34","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1016\/j.neuron.2022.12.025","type":"journal-article","article-title":"Humidity sensors that alert mosquitoes to nearby hosts and egg-laying sites","volume":"111","author":"Laursen","year":"2023","unstructured":"Laursen, W. J. et al. Humidity sensors that alert mosquitoes to nearby hosts and egg-laying sites. Neuron. 111 (6), 874-887.e8 (2023).","journal-title":"Neuron"},{"issue":"11","key":"ref35","doi-asserted-by":"crossref","first-page":"e1011065","DOI":"10.1371\/journal.pgen.1011065","type":"journal-article","article-title":"Efficient sex separation by exploiting differential alternative splicing of a dominant marker in Aedes aegypti","volume":"19","author":"Weng","year":"2023","unstructured":"Weng, S.-C., Antoshechkin, I., Marois, E., Akbari, O. S. Efficient sex separation by exploiting differential alternative splicing of a dominant marker in Aedes aegypti. PLoS Genet. 19 (11), e1011065 (2023).","journal-title":"PLoS Genet"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"e51701","DOI":"10.7554\/eLife.51701","type":"journal-article","article-title":"Development of a confinable gene drive system in the human disease vector","volume":"9","author":"Li","year":"2020","unstructured":"Li, M. et al. Development of a confinable gene drive system in the human disease vector. eLife. 9, e51701 (2020).","journal-title":"eLife"},{"key":"ref37","type":"book","article-title":"Engineered Antiviral Sensor Targets Infected Mosquitoes","volume-title":"The CRISPR journal","author":"Dalla Benetta","year":"2023","unstructured":"Dalla Benetta, E. et al. Engineered Antiviral Sensor Targets Infected Mosquitoes. The CRISPR journal. 6 (6), 543\u2013556 (2023)."},{"issue":"9","key":"ref38","doi-asserted-by":"crossref","first-page":"101486","DOI":"10.1016\/j.isci.2020.101486","type":"journal-article","article-title":"C-Type lectins link immunological and reproductive processes in Aedes aegypti","volume":"23","author":"Li","year":"2020","unstructured":"Li, H.-H. et al. C-Type lectins link immunological and reproductive processes in Aedes aegypti. iScience. 23 (9), 101486 (2020).","journal-title":"iScience"},{"issue":"Pt 8","key":"ref39","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1099\/0022-1317-80-8-2253","type":"journal-article","article-title":"Role of the 3' untranslated region of baculovirus p10 mRNA in high-level expression of foreign genes","volume":"80","author":"van Oers","year":"1999","unstructured":"van Oers, M. M., Vlak, J. M., Voorma, H. O., Thomas, A. A. M. Role of the 3' untranslated region of baculovirus p10 mRNA in high-level expression of foreign genes. J Gen Virol. 80 (Pt 8), 2253-2262 (1999).","journal-title":"J Gen Virol"},{"issue":"12","key":"ref40","doi-asserted-by":"crossref","first-page":"e0145019","DOI":"10.1371\/journal.pone.0145019","type":"journal-article","article-title":"The influence of SV40 polyA on gene expression of baculovirus expression vector systems","volume":"10","author":"Salem","year":"2015","unstructured":"Salem, T. Z. et al. The influence of SV40 polyA on gene expression of baculovirus expression vector systems. PloS One. 10 (12), e0145019 (2015).","journal-title":"PloS One"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/67732\/genome-editing-yellow-fever-mosquito-aedes-aegypti-using-crispr","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:35:21Z","timestamp":1792596921000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/67732\/genome-editing-yellow-fever-mosquito-aedes-aegypti-using-crispr"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,21]]},"references-count":40,"journal-issue":{"issue":"217","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/67732","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,21]]}}}