{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:47:27Z","timestamp":1792597647508,"version":"4.5.0"},"reference-count":34,"publisher":"MyJove Corporation","issue":"220","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/67792","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T19:02:00Z","timestamp":1749841320000},"source":"Crossref","is-referenced-by-count":1,"title":["Rapid Detection of <em>Helicobacter pylori<\/em> Virulence and Typing Using Quantum Dot Labeling Technology"],"prefix":"10.3791","author":[{"given":"Lu-Si","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Nan","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Zhen","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Feng","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Xiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Jing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang-Feng","family":"Ou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan-Huan","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Yan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Quan","family":"Gan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,6,13]]},"reference":[{"issue":"3","key":"ref1","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/S1473-3099(17)30753-3","type":"journal-article","article-title":"research and development of new antibiotics: WHO priority list of antibioticresistant bacteria and tuberculosis","volume":"18","author":"Tacconelli","year":"2018","unstructured":"Tacconelli, E. et al. Discovery, research and development of new antibiotics: WHO priority list of antibioticresistant bacteria and tuberculosis. Lancet Infect Dis. 18 (3), 318-327 (2018).","journal-title":"Lancet Infect Dis"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.mayocp.2016.11.017","type":"journal-article","article-title":"Helicobacter pylori: past, present, and future of treatment","volume":"92","author":"Kamboj","year":"2017","unstructured":"Kamboj, A. K. et al. Helicobacter pylori: past, present, and future of treatment. Mayo Clin Proc. 92 (4), 599-604 (2017).","journal-title":"Mayo Clin Proc"},{"issue":"11","key":"ref3","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1038\/nrgastro.2010.154","type":"journal-article","article-title":"Disease mechanisms: Helicobacter pylori virulence factors","volume":"7","author":"Yamaoka","year":"2010","unstructured":"Yamaoka, Y. Disease mechanisms: Helicobacter pylori virulence factors. Nat Rev Gastroenterol Hepatol. 7 (11), 629-641 (2010).","journal-title":"Nat Rev Gastroenterol Hepatol"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s42770-021-00675-0","type":"journal-article","article-title":"Helicobacter pylori: an updated overview on its virulence and pathogenesis","volume":"53","author":"Sharndama","year":"2022","unstructured":"Sharndama, H. C., Mba, I. E. Helicobacter pylori: an updated overview on its virulence and pathogenesis. Braz J Microbiol. 53 (1), 33-50 (2022).","journal-title":"Braz J Microbiol"},{"issue":"25","key":"ref5","doi-asserted-by":"crossref","first-page":"3673","DOI":"10.3748\/wjg.v26.i25.3673","type":"journal-article","article-title":"Infection status of type I and type II Helicobacter pylori in areas with high incidence of gastric cancer and its impact on gastrin and pepsinogen levels","volume":"26","author":"Lin","year":"2020","unstructured":"Lin, Y. et al. Infection status of type I and type II Helicobacter pylori in areas with high incidence of gastric cancer and its impact on gastrin and pepsinogen levels. World J Gastroenterol. 26 (25), 3673-3685 (2020).","journal-title":"World J Gastroenterol"},{"issue":"6","key":"ref6","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/S2468-1253(23)00070-5","type":"journal-article","article-title":"Global prevalence of Helicobacter pylori infection from 1980 to 2022: a systematic review and metaanalysis","volume":"8","author":"Li","year":"2023","unstructured":"Li, Y. H. et al. Global prevalence of Helicobacter pylori infection from 1980 to 2022: a systematic review and metaanalysis. Lancet Gastroenterol Hepatol. 8 (6), 553-564 (2023).","journal-title":"Lancet Gastroenterol Hepatol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"100904","DOI":"10.1016\/j.nmni.2021.100904","type":"journal-article","article-title":"Rescue effect of lactobacillicontaining bismuth regimen on Helicobacter pylori treatment failure","volume":"42","author":"Karbalaei","year":"2021","unstructured":"Karbalaei, M., Keikha, M. Rescue effect of lactobacillicontaining bismuth regimen on Helicobacter pylori treatment failure. New Microbiol New Infect. 42, 100904 (2021).","journal-title":"New Microbiol New Infect"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/s41572-023-00431-8","type":"journal-article","article-title":"Helicobacter pylori infection","volume":"9","author":"Malfertheiner","year":"2023","unstructured":"Malfertheiner, P. et al. Helicobacter pylori infection. Nat Rev Dis Primers. 9 (1), 19 (2023).","journal-title":"Nat Rev Dis Primers"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"e9","DOI":"10.1002\/bjs.9329","type":"journal-article","article-title":"Global burden of disease requiring emergency surgery","volume":"101","author":"Stewart","year":"2014","unstructured":"Stewart, B. et al. Global burden of disease requiring emergency surgery. Br J Surg. 101 (1), e9-e22 (2014).","journal-title":"Br J Surg"},{"issue":"2","key":"ref10","doi-asserted-by":"crossref","first-page":"e180","DOI":"10.1016\/S2214-109X(19)30488-7","type":"journal-article","article-title":"The global burden of cancer attributable to infection in 2018: a global incidence analysis","volume":"8","author":"de Martel","year":"2020","unstructured":"de Martel, C. et al. The global burden of cancer attributable to infection in 2018: a global incidence analysis. Lancet Glob Health. 8 (2), e180-e190 (2020).","journal-title":"Lancet Glob Health"},{"issue":"S1","key":"ref11","doi-asserted-by":"crossref","first-page":"e12735","DOI":"10.1111\/hel.12735","type":"journal-article","article-title":"Review: diagnosis of Helicobacter pylori infection","volume":"25","author":"Godbole","year":"2020","unstructured":"Godbole, G. et al. Review: diagnosis of Helicobacter pylori infection. Helicobacter. 25 (S1), e12735 (2020).","journal-title":"Helicobacter"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1146\/annurev-med-042220-020814","type":"journal-article","article-title":"Diagnosis and treatment of Helicobacter pylori infection","volume":"73","author":"Lee","year":"2022","unstructured":"Lee, Y. C. et al. Diagnosis and treatment of Helicobacter pylori infection. Annu Rev Med. 73 (1), 183-195 (2022).","journal-title":"Annu Rev Med"},{"key":"ref13","first-page":"429","type":"journal-article","article-title":"Helicobacter pylori infection","volume":"115","author":"Fischbach","year":"2018","unstructured":"Fischbach, W., Malfertheiner, P. Helicobacter pylori infection. Ger Int Med J. 115, 429-436 (2018).","journal-title":"Ger Int Med J"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"e24069","DOI":"10.1002\/jcla.24069","type":"journal-article","article-title":"Survey on the quantitative detection of serum Helicobacter pylori antibodies in clinical laboratories in my country","volume":"36","author":"Zhang","year":"2021","unstructured":"Zhang, C. et al. Survey on the quantitative detection of serum Helicobacter pylori antibodies in clinical laboratories in my country. J Clin Lab Anal. 36 (1), e24069 (2021).","journal-title":"J Clin Lab Anal"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1229871","DOI":"10.3389\/fonc.2023.1229871","type":"journal-article","article-title":"Improving the detection rate of Helicobacter pylori in chronic gastritis biopsies: a comparative analysis of H&E, methylene blue, WarthinStarry, immunohistochemistry, and quantum dot immunohistochemistry","volume":"13","author":"Wan","year":"2023","unstructured":"Wan, W. S. et al. Improving the detection rate of Helicobacter pylori in chronic gastritis biopsies: a comparative analysis of H&E, methylene blue, WarthinStarry, immunohistochemistry, and quantum dot immunohistochemistry. Front Oncol. 13, 1229871 (2023).","journal-title":"Front Oncol"},{"issue":"7","key":"ref16","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1369\/jhc.2009.953547","type":"journal-article","article-title":"Quantitative in situ detection of phosphoproteins in fixed tissues using quantum dot technology","volume":"57","author":"Bodo","year":"2009","unstructured":"Bodo, J. et al. Quantitative in situ detection of phosphoproteins in fixed tissues using quantum dot technology. J Histochem Cytochem. 57 (7), 701-708 (2009).","journal-title":"J Histochem Cytochem"},{"issue":"15","key":"ref17","doi-asserted-by":"crossref","first-page":"2912","DOI":"10.1016\/j.biomaterials.2009.02.010","type":"journal-article","article-title":"Quantum dotbased immunofluorescence technology for quantitative determination of HER2 expression in breast cancer","volume":"30","author":"Chen","year":"2009","unstructured":"Chen, C. et al. Quantum dotbased immunofluorescence technology for quantitative determination of HER2 expression in breast cancer. Biomaterials. 30 (15), 2912-2918 (2009).","journal-title":"Biomaterials"},{"issue":"10","key":"ref18","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.1016\/j.trac.2011.04.022","type":"journal-article","article-title":"Recent developments in quantum dot analytical applications","volume":"30","author":"Kuang","year":"2011","unstructured":"Kuang, H. et al. Recent developments in quantum dot analytical applications. TrAC Trends Anal Chem. 30 (10), 1620-1636 (2011).","journal-title":"TrAC Trends Anal Chem"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.jphotochemrev.2017.01.002","type":"journal-article","article-title":"Medically transformable quantum dots for biosensing and imaging","volume":"30","author":"Park","year":"2017","unstructured":"Park, Y. et al. Medically transformable quantum dots for biosensing and imaging. J Photochem Photobiol C Photochem Rev. 30, 51-70 (2017).","journal-title":"J Photochem Photobiol C Photochem Rev"},{"issue":"14","key":"ref20","doi-asserted-by":"crossref","first-page":"2501","DOI":"10.3390\/nano12142501","type":"journal-article","article-title":"Semiconductor quantum dots as target analytes: properties, surface chemistry and detection","volume":"12","author":"SanmartinMatalbobs","year":"2022","unstructured":"SanmartinMatalbobs, J. et al. Semiconductor quantum dots as target analytes: properties, surface chemistry and detection. Chem Detect Nanomaterials. 12 (14), 2501 (2022).","journal-title":"Chem Detect Nanomaterials"},{"issue":"5","key":"ref21","doi-asserted-by":"crossref","first-page":"3140","DOI":"10.1021\/acs.chemrev.8b00417","type":"journal-article","article-title":"Quantum and dielectric confinement effects in lowerdimensional hybrid perovskite semiconductors","volume":"119","author":"Katan","year":"2019","unstructured":"Katan, C., Mercier, N., Even, J. Quantum and dielectric confinement effects in lowerdimensional hybrid perovskite semiconductors. Chem Rev. 119 (5), 3140-3192 (2019).","journal-title":"Chem Rev"},{"issue":"2","key":"ref22","first-page":"94","type":"journal-article","article-title":"Physical examination population 13C expert consensus on technical specifications for urea breath tests","volume":"2","year":"2021","unstructured":"Chinese Medical Association Branch of Health Management. Physical examination population 13C expert consensus on technical specifications for urea breath tests. Health Exam Manag. 2 (2), 94-96 (2021).","journal-title":"Health Exam Manag"},{"issue":"2020","key":"ref23","first-page":"510","type":"journal-article","article-title":"Expert consensus on clinical application of Helicobacter pyloriurea breath test (2020)","volume":"14","year":"2020","unstructured":"National Clinical Medical Research Center for Digestive Diseases. Expert consensus on clinical application of Helicobacter pyloriurea breath test (2020). Chin J Health Manag. 14 (6), 510-513 (2020).","journal-title":"Chin J Health Manag"},{"issue":"3","key":"ref24","first-page":"197","type":"journal-article","article-title":"The influence factors of Helicobacter pylori infection were detected by urea breath test","volume":"32","author":"Zhang","year":"2020","unstructured":"Zhang, Y. et al. The influence factors of Helicobacter pylori infection were detected by urea breath test. Chin J Clin Gastroenterol. 32 (3), 197-202 (2020).","journal-title":"Chin J Clin Gastroenterol"},{"issue":"1","key":"ref25","first-page":"74","type":"journal-article","article-title":"Diagnostic value of gastric function triad and Helicobacter pylori typing in chronic gastritis and gastric cancer","volume":"30","author":"Lin","year":"2023","unstructured":"Lin, B. et al. Diagnostic value of gastric function triad and Helicobacter pylori typing in chronic gastritis and gastric cancer. Labeled Immunoassays Clin Med. 30 (1), 74-76 (2023).","journal-title":"Labeled Immunoassays Clin Med"},{"issue":"508","key":"ref26","first-page":"1","type":"journal-article","article-title":"Evolution of diagnostic methods for Helicobacter pylori infection: from traditional tests to hightech, advanced sensitivity and identification tools","volume":"12","author":"Cardos","year":"2022","unstructured":"Cardos, A. I. et al. Evolution of diagnostic methods for Helicobacter pylori infection: from traditional tests to hightech, advanced sensitivity and identification tools. Diagnostics. 12 (508), 1-23 (2022).","journal-title":"Diagnostics"},{"issue":"2","key":"ref27","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1136\/gutjnl-2021-325630","type":"journal-article","article-title":"Chinese consensus report on familybased Helicobacter pylori infection control and management (2021 edition)","volume":"71","author":"Ding","year":"2021","unstructured":"Ding, S. Z. et al. Chinese consensus report on familybased Helicobacter pylori infection control and management (2021 edition). Gut. 71 (2), 238-253 (2021).","journal-title":"Gut"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1007\/s10735-020-09954-w","type":"journal-article","article-title":"Comparison of quantum dot immunofluorescence histochemistry and conventional immunohistochemistry in detecting Helicobacter pylori infection in paraffinembedded tissue of gastric biopsy","volume":"52","author":"Wan","year":"2021","unstructured":"Wan, W. et al. Comparison of quantum dot immunofluorescence histochemistry and conventional immunohistochemistry in detecting Helicobacter pylori infection in paraffinembedded tissue of gastric biopsy. J Mol Histol. 52, 461-466 (2021).","journal-title":"J Mol Histol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"130139","DOI":"10.1016\/j.snb.2021.130139","type":"journal-article","article-title":"Development of a fast and sensitive doubleantigen sandwich lateral flow immunoassay based on quantum dot nanobeads and its clinical performance in detecting SARSCoV2 total antibodies","volume":"343","author":"Zhou","year":"2021","unstructured":"Zhou, Y. et al. Development of a fast and sensitive doubleantigen sandwich lateral flow immunoassay based on quantum dot nanobeads and its clinical performance in detecting SARSCoV2 total antibodies. Sens Actuators B Chem. 343, 130139 (2021).","journal-title":"Sens Actuators B Chem"},{"issue":"4","key":"ref30","first-page":"1267","type":"journal-article","article-title":"Consistency analysis of highsensitivity cardiac troponin I in peripheral blood and venous blood using quantum dot immunofluorescence method and its clinical application in acute myocardial infarction","volume":"14","author":"Liu","year":"2022","unstructured":"Liu, H. et al. Consistency analysis of highsensitivity cardiac troponin I in peripheral blood and venous blood using quantum dot immunofluorescence method and its clinical application in acute myocardial infarction. J Chest Dis. 14 (4), 1267-1274 (2022).","journal-title":"J Chest Dis"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"22253","DOI":"10.1038\/s41598-023-49244-5","type":"journal-article","article-title":"Development of quantum dotbased lateral flow immunoassay test strips for rapid and sensitive detection of SARSCoV2 neutralizing antibodies","volume":"13","author":"Xirong","year":"2023","unstructured":"Xirong, W. et al. Development of quantum dotbased lateral flow immunoassay test strips for rapid and sensitive detection of SARSCoV2 neutralizing antibodies. Sci Rep. 13, 22253 (2023).","journal-title":"Sci Rep"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"132044","DOI":"10.1016\/j.snb.2022.132044","type":"journal-article","article-title":"Programmable graphenebased microfluidic sensor for DNA detection","volume":"367","author":"Purwidyantri","year":"2022","unstructured":"Purwidyantri, A. et al. Programmable graphenebased microfluidic sensor for DNA detection. Sens Actuators B Chem. 367, 132044 (2022).","journal-title":"Sens Actuators B Chem"},{"issue":"3","key":"ref33","doi-asserted-by":"crossref","first-page":"62","DOI":"10.15698\/mic2020.03.709","type":"journal-article","article-title":"Ramanbased sorting of microbial cells to link functions to their genes","volume":"7","author":"Lee","year":"2020","unstructured":"Lee, K. S., Wagner, M., Stocker, R. Ramanbased sorting of microbial cells to link functions to their genes. Microb Cell. 7 (3), 62-65 (2020).","journal-title":"Microb Cell"},{"issue":"8","key":"ref34","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1039\/C8CS00750K","type":"journal-article","article-title":"Design and fabrication of carbon dots for energy conversion and storage","volume":"48","author":"Hu","year":"2019","unstructured":"Hu, C. et al. Design and fabrication of carbon dots for energy conversion and storage. Chem Soc Rev. 48 (8), 2315-2337 (2019).","journal-title":"Chem Soc Rev"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/67792\/rapid-detection-helicobacter-pylori-virulence-typing-using-quantum","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:58:38Z","timestamp":1792594718000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/67792\/rapid-detection-helicobacter-pylori-virulence-typing-using-quantum"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,13]]},"references-count":34,"journal-issue":{"issue":"220","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/67792","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,13]]}}}