{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:56:48Z","timestamp":1792598208848,"version":"4.5.0"},"reference-count":15,"publisher":"MyJove Corporation","issue":"217","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/67824","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T19:06:23Z","timestamp":1743188783000},"source":"Crossref","is-referenced-by-count":1,"title":["Analyzing <em>Ex Vivo<\/em> Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow Monocytes"],"prefix":"10.3791","author":[{"given":"Erin B.","family":"Taylor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Spitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine R.","family":"O'Quinn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan J.","family":"Mouton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,3,28]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.trsl.2017.10.001","type":"journal-article","article-title":"Cardiac macrophage biology in the steady-state heart, the aging heart, and following myocardial infarction","volume":"191","author":"Ma","year":"2018","unstructured":"Ma, Y., Mouton, A. J., Lindsey, M. L. Cardiac macrophage biology in the steady-state heart, the aging heart, and following myocardial infarction. Transl Res. 191, 15-28 (2018).","journal-title":"Transl Res"},{"issue":"6","key":"ref2","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1161\/CIRCRESAHA.119.312321","type":"journal-article","article-title":"Obesity, hypertension, and cardiac dysfunction: Novel roles of immunometabolism in macrophage activation and inflammation","volume":"126","author":"Mouton","year":"2020","unstructured":"Mouton, A. J., Li, X., Hall, M. E., Hall, J. E. Obesity, hypertension, and cardiac dysfunction: Novel roles of immunometabolism in macrophage activation and inflammation. Circ Res. 126 (6), 789-806 (2020).","journal-title":"Circ Res"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.yjmcc.2021.05.008","type":"journal-article","article-title":"Dimethyl fumarate preserves left ventricular infarct integrity following myocardial infarction via modulation of cardiac macrophage and fibroblast oxidative metabolism","volume":"158","author":"Mouton","year":"2021","unstructured":"Mouton, A. J. et al. Dimethyl fumarate preserves left ventricular infarct integrity following myocardial infarction via modulation of cardiac macrophage and fibroblast oxidative metabolism. J Mol Cell Cardiol. 158, 38-48 (2021).","journal-title":"J Mol Cell Cardiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1136252","DOI":"10.3389\/fcvm.2023.1136252","type":"journal-article","article-title":"Temporal changes in glucose metabolism reflect polarization in resident and monocyte-derived macrophages after myocardial infarction","volume":"10","author":"Mouton","year":"2023","unstructured":"Mouton, A. J. et al. Temporal changes in glucose metabolism reflect polarization in resident and monocyte-derived macrophages after myocardial infarction. Front Cardiovasc Med. 10, 1136252 (2023).","journal-title":"Front Cardiovasc Med"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"C571","DOI":"10.1152\/ajpcell.00272.2024","type":"journal-article","article-title":"Glutamine metabolism improves left ventricular function but not macrophage-mediated inflammation following myocardial infarction","volume":"327","author":"Mouton","year":"2024","unstructured":"Mouton, A. J. et al. Glutamine metabolism improves left ventricular function but not macrophage-mediated inflammation following myocardial infarction. Am J Physiol Cell Physiol. 327 (3), C571-C586 (2024).","journal-title":"Am J Physiol Cell Physiol"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1007\/s00395-018-0686-x","type":"journal-article","article-title":"Mapping macrophage polarization over the myocardial infarction time continuum","volume":"113","author":"Mouton","year":"2018","unstructured":"Mouton, A. J. et al. Mapping macrophage polarization over the myocardial infarction time continuum. Basic Res Cardiol. 113 (4), 26 (2018).","journal-title":"Basic Res Cardiol"},{"key":"ref7","volume-title":"XF software. Agilent Seahorse Analytics","year":"2025","unstructured":"Agilent Technologies, Inc. XF software. Agilent Seahorse Analytics. https:\/\/www.agilent.com\/en\/product\/cell-analysis\/real-time-cell-metabolic-analysis\/xf-software\/agilent-seahorse-analytics-787485 (2025)."},{"issue":"5","key":"ref8","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1161\/CIRCRESAHA.121.320060","type":"journal-article","article-title":"Diabetes suppresses glucose uptake and glycolysis in macrophages","volume":"130","author":"Matsuura","year":"2022","unstructured":"Matsuura, Y. et al. Diabetes suppresses glucose uptake and glycolysis in macrophages. Circ Res. 130 (5), 779-781 (2022).","journal-title":"Circ Res"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"e54090","DOI":"10.7554\/eLife.54090","type":"journal-article","article-title":"Bmal1 integrates mitochondrial metabolism and macrophage activation","volume":"9","author":"Alexander","year":"2020","unstructured":"Alexander, R. K. et al. Bmal1 integrates mitochondrial metabolism and macrophage activation. Elife. 9, e54090 (2020).","journal-title":"Elife"},{"issue":"4","key":"ref10","doi-asserted-by":"crossref","first-page":"e0089","DOI":"10.1097\/HC9.0000000000000089","type":"journal-article","article-title":"miR-34a regulates macrophage-associated inflammation and angiogenesis in alcohol-induced liver injury","volume":"7","author":"Wan","year":"2023","unstructured":"Wan, Y. et al. miR-34a regulates macrophage-associated inflammation and angiogenesis in alcohol-induced liver injury. Hepatol Commun. 7 (4), e0089 (2023).","journal-title":"Hepatol Commun"},{"issue":"3","key":"ref11","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1038\/ni.3366","type":"journal-article","article-title":"Fatty acid oxidation in macrophage polarization","volume":"17","author":"Nomura","year":"2016","unstructured":"Nomura, M. et al. Fatty acid oxidation in macrophage polarization. Nat Immunol. 17 (3), 216-217 (2016).","journal-title":"Nat Immunol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1199751","DOI":"10.3389\/fimmu.2023.1199751","type":"journal-article","article-title":"Glycolytic reprogramming in macrophages and MSCs during inflammation","volume":"14","author":"Li","year":"2023","unstructured":"Li, X. et al. Glycolytic reprogramming in macrophages and MSCs during inflammation. Front Immunol. 14, 1199751 (2023).","journal-title":"Front Immunol"},{"issue":"10","key":"ref13","doi-asserted-by":"crossref","first-page":"e56380","DOI":"10.15252\/embr.202256380","type":"journal-article","article-title":"Calculation of ATP production rates using the Seahorse XF Analyzer","volume":"24","author":"Desousa","year":"2023","unstructured":"Desousa, B. R. et al. Calculation of ATP production rates using the Seahorse XF Analyzer. EMBO Rep. 24 (10), e56380 (2023).","journal-title":"EMBO Rep"},{"issue":"8","key":"ref14","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1161\/CIRCRESAHA.117.312535","type":"journal-article","article-title":"Noninvasive immunometabolic cardiac inflammation imaging using hyperpolarized magnetic resonance","volume":"122","author":"Lewis","year":"2018","unstructured":"Lewis, A. J. M. et al. Noninvasive immunometabolic cardiac inflammation imaging using hyperpolarized magnetic resonance. Circ Res. 122 (8), 1084-1093 (2018).","journal-title":"Circ Res"},{"key":"ref15","type":"book","article-title":"Assessing T cell bioenergetic poise and spare respiratory capacity using extracellular flux analysis","volume-title":"Agilent Technologies application note","author":"Walls","year":"2022","unstructured":"Walls, J., Romero, M. Assessing T cell bioenergetic poise and spare respiratory capacity using extracellular flux analysis. Agilent Technologies application note. Publication number: 5994-4494EN (2022)."}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/67824\/analyzing-ex-vivo-metabolic-flux-splenic-cardiac-macrophages-bone","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:33:09Z","timestamp":1792596789000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/67824\/analyzing-ex-vivo-metabolic-flux-splenic-cardiac-macrophages-bone"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,28]]},"references-count":15,"journal-issue":{"issue":"217","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/67824","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,28]]}}}