{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:39:43Z","timestamp":1792597183285,"version":"4.5.0"},"reference-count":18,"publisher":"MyJove Corporation","issue":"222","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/68290","type":"journal-article","created":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T23:00:32Z","timestamp":1754089232000},"source":"Crossref","is-referenced-by-count":0,"title":["<em>In Ovo<\/em> Xenografting of Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells (PDX-ALL)"],"prefix":"10.3791","author":[{"given":"Jung Kwon","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian","family":"Willetts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Storek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl","family":"Riabowol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myung Yung","family":"Jeong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki-Young","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,8,1]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1093\/jnci\/82.1.4","type":"journal-article","article-title":"What is the evidence that tumors are angiogenesis dependent","volume":"82","author":"Folkman","year":"1990","unstructured":"Folkman, J. What is the evidence that tumors are angiogenesis dependent? J Natl Cancer Inst. 82 (1), 4-6 (1990).","journal-title":"J Natl Cancer Inst"},{"issue":"5","key":"ref2","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1073\/pnas.0408622102","type":"journal-article","article-title":"Accessing key steps of human tumor progression in vivo by using an avian embryo model","volume":"102","author":"Hagedorn","year":"2005","unstructured":"Hagedorn, M. et al. Accessing key steps of human tumor progression in vivo by using an avian embryo model. Proc Natl Acad Sci U S A. 102 (5), 1643-1648 (2005).","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"ref3","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1053\/j.gastro.2009.12.061","type":"journal-article","article-title":"Netrin-1 mediates early events in pancreatic adenocarcinoma progression, acting on tumor and endothelial cells","volume":"138","author":"Dumartin","year":"2010","unstructured":"Dumartin, L. et al. Netrin-1 mediates early events in pancreatic adenocarcinoma progression, acting on tumor and endothelial cells. Gastroenterology. 138 (5), 1595-1606 (2010).","journal-title":"Gastroenterology"},{"issue":"7","key":"ref4","first-page":"1684","type":"journal-article","article-title":"ERK activity as a determinant of tumor growth and dormancy: Regulation by p38","volume":"63","author":"Aguirre-Ghiso","year":"2003","unstructured":"Aguirre-Ghiso, J. A., Estrada, Y., Liu, D., Ossowski, L. ERK activity as a determinant of tumor growth and dormancy: Regulation by p38. Cancer Res. 63 (7), 1684-1695 (2003).","journal-title":"Cancer Res"},{"key":"ref5","first-page":"227","type":"journal-article","article-title":"Ribonomic and short hairpin RNA gene silencing methods to explore functional gene programs associated with tumor growth arrest","volume":"383","author":"Baroni","year":"2007","unstructured":"Baroni, T. E. et al. Ribonomic and short hairpin RNA gene silencing methods to explore functional gene programs associated with tumor growth arrest. Methods Mol Biol. 383, 227-244 (2007).","journal-title":"Methods Mol Biol"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1158\/0008-5472.CAN-13-0588","type":"journal-article","article-title":"Inducible nitric oxide synthase drives mTOR pathway activation and proliferation of human melanoma by reversible nitrosylation of TSC2","volume":"74","author":"Lopez-Rivera","year":"2014","unstructured":"Lopez-Rivera, E. et al. Inducible nitric oxide synthase drives mTOR pathway activation and proliferation of human melanoma by reversible nitrosylation of TSC2. Cancer Res. 74 (4), 1067-1078 (2014).","journal-title":"Cancer Res"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1186\/1471-2407-11-241","type":"journal-article","article-title":"A short-term in vivo model for giant cell tumor of bone","volume":"11","author":"Balke","year":"2011","unstructured":"Balke, M. et al. A short-term in vivo model for giant cell tumor of bone. BMC Cancer. 11, 241 (2011).","journal-title":"BMC Cancer"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/1756-0500-3-58","type":"journal-article","article-title":"Morphologic characterization of osteosarcoma growth on the chick chorioallantoic membrane","volume":"3","author":"Balke","year":"2010","unstructured":"Balke, M. et al. Morphologic characterization of osteosarcoma growth on the chick chorioallantoic membrane. BMC Res Notes. 3, 58 (2010).","journal-title":"BMC Res Notes"},{"issue":"77","key":"ref9","first-page":"e50522","type":"journal-article","article-title":"The CAM-assay as a xenograft model for sarcoma","author":"Sys","year":"2013","unstructured":"Sys, G. M. L. et al. The CAM-assay as a xenograft model for sarcoma. J Vis Exp. 77, e50522 (2013).","journal-title":"J Vis Exp"},{"issue":"104","key":"ref10","first-page":"e52411","type":"journal-article","article-title":"The Chick Chorioallantoic Membrane (CAM) assay as an efficient xenograft model of hepatocellular carcinoma","author":"Li","year":"2015","unstructured":"Li, M. et al. The Chick Chorioallantoic Membrane (CAM) assay as an efficient xenograft model of hepatocellular carcinoma. J Vis Exp. 104, e52411 (2015).","journal-title":"J Vis Exp"},{"key":"ref11","type":"journal-article","article-title":"Acute lymphoblastic leukemia necessitates GSH-dependent ferroptosis defenses to overcome FSP1-epigenetic silencing","volume":"102408","author":"Pontel","year":"2022","unstructured":"Pontel, L. B. et al. Acute lymphoblastic leukemia necessitates GSH-dependent ferroptosis defenses to overcome FSP1-epigenetic silencing. Redox Biol. 102408 (2022).","journal-title":"Redox Biol"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"589","DOI":"10.3324\/haematol.2009.014274","type":"journal-article","article-title":"Outcome after relapse of acute lymphoblastic leukemia in adult patients included in four consecutive risk-adapted trials by the PETHEMA Study Group","volume":"95","author":"Oriol","year":"2010","unstructured":"Oriol, A. et al. Outcome after relapse of acute lymphoblastic leukemia in adult patients included in four consecutive risk-adapted trials by the PETHEMA Study Group. Haematologica. 95 (4), 589-596 (2010).","journal-title":"Haematologica"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"114970","DOI":"10.1016\/j.bcp.2022.114970","type":"journal-article","article-title":"Animal models of acute lymphoblastic leukemia: Recapitulating the human disease to evaluate drug efficacy and discover therapeutic targets","volume":"198","author":"Xin","year":"2022","unstructured":"Xin, Q., Chen, Z., Wei, W., Wu, Y. Animal models of acute lymphoblastic leukemia: Recapitulating the human disease to evaluate drug efficacy and discover therapeutic targets. Biochem Pharmacol. 198, 114970 (2022).","journal-title":"Biochem Pharmacol"},{"issue":"4","key":"ref14","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1111\/j.1365-2141.1982.tb01962.x","type":"journal-article","article-title":"A technique for rapid isolation of bone marrow mononuclear cells using Ficoll-Metrizoate and the IBM 2991 blood cell processor","volume":"50","author":"Gilmore","year":"1982","unstructured":"Gilmore, M. J., Prentice, H. G., Blacklock, H. A., Janossy, G., Hoffbrand, A. V. A technique for rapid isolation of bone marrow mononuclear cells using Ficoll-Metrizoate and the IBM 2991 blood cell processor. Br J Haematol. 50 (4), 619-626 (1982).","journal-title":"Br J Haematol"},{"issue":"133","key":"ref15","first-page":"e56976","type":"journal-article","article-title":"Flow cytometry to estimate leukemia stem cells in primary acute myeloid leukemia and in patient-derived xenografts, at diagnosis and follow-up","author":"Boyer","year":"2018","unstructured":"Boyer, T. et al. Flow cytometry to estimate leukemia stem cells in primary acute myeloid leukemia and in patient-derived xenografts, at diagnosis and follow-up. J Vis Exp. 133, e56976 (2018).","journal-title":"J Vis Exp"},{"issue":"42","key":"ref16","doi-asserted-by":"crossref","first-page":"5910","DOI":"10.1038\/onc.2017.211","type":"journal-article","article-title":"Genome-wide loss-of-function genetic screening identifies opioid receptor mu1 as a key regulator of L-asparaginase resistance in pediatric acute lymphoblastic leukemia","volume":"36","author":"Kang","year":"2017","unstructured":"Kang, S. M., et al. Genome-wide loss-of-function genetic screening identifies opioid receptor mu1 as a key regulator of L-asparaginase resistance in pediatric acute lymphoblastic leukemia. Oncogene. 36 (42), 5910-5913 (2017).","journal-title":"Oncogene"},{"issue":"20","key":"ref17","doi-asserted-by":"crossref","first-page":"2222","DOI":"10.1182\/blood-2018-12-890236","type":"journal-article","article-title":"HAP1 loss confers L-asparaginase resistance in ALL by downregulating the calpain-1-Bid-caspase-3\/12 pathway","volume":"133","author":"Lee","year":"2019","unstructured":"Lee, J. K., et al. HAP1 loss confers L-asparaginase resistance in ALL by downregulating the calpain-1-Bid-caspase-3\/12 pathway. Blood. 133 (20), 2222-2232 (2019).","journal-title":"Blood"},{"issue":"9","key":"ref18","doi-asserted-by":"crossref","first-page":"1986","DOI":"10.1038\/leu.2012.122","type":"journal-article","article-title":"EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols","volume":"26","author":"Kalina","year":"2012","unstructured":"Kalina, T. et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 26 (9), 1986-2010 (2012).","journal-title":"Leukemia"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/68290\/in-ovo-xenografting-patient-derived-acute-lymphoblastic-leukemia-all","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:42:38Z","timestamp":1792593758000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/68290\/in-ovo-xenografting-patient-derived-acute-lymphoblastic-leukemia-all"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,1]]},"references-count":18,"journal-issue":{"issue":"222","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/68290","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,1]]}}}