{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:43:30Z","timestamp":1792597410748,"version":"4.5.0"},"reference-count":28,"publisher":"MyJove Corporation","issue":"221","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/68325","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T18:02:57Z","timestamp":1753984977000},"source":"Crossref","is-referenced-by-count":0,"title":["Gut Transit Defects in &lt;em&gt;Drosophila&lt;\/em&gt; Models of Monogenic Parkinson's Disease Using a Constipation Assay"],"prefix":"10.3791","author":[{"given":"Melissa","family":"Vos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuliia","family":"Kanana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,7,11]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00415-024-12730-5","type":"journal-article","article-title":"Diagnosis and treatment of autonomic failure, pain and sleep disturbances in Parkinson's disease: Guideline &#34;Parkinson's disease&#34 of the German Society of Neurology","volume":"272","author":"Fanciulli","year":"2025","unstructured":"Fanciulli, A. et al. Diagnosis and treatment of autonomic failure, pain and sleep disturbances in Parkinson's disease: Guideline \"Parkinson's disease\" of the German Society of Neurology. J Neurol. 272 (1), 1-32 (2025).","journal-title":"J Neurol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/bs.irn.2017.05.030","type":"journal-article","article-title":"Nonmotor signs in genetic forms of Parkinson's disease","volume":"133","author":"Kasten","year":"2017","unstructured":"Kasten, M., Marras, C., Klein, C. Nonmotor signs in genetic forms of Parkinson's disease. Int Rev Neurobiol. 133, 129-178 (2017).","journal-title":"Int Rev Neurobiol"},{"issue":"10291","key":"ref3","doi-asserted-by":"crossref","first-page":"2284","DOI":"10.1016\/S0140-6736(21)00218-X","type":"journal-article","article-title":"Parkinson's disease","volume":"397","author":"Bloem","year":"2021","unstructured":"Bloem, B. R., Okun, M. S., Klein, C. Parkinson's disease. Lancet. 397 (10291), 2284-2303 (2021).","journal-title":"Lancet"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1158986","DOI":"10.3389\/fcimb.2023.1158986","type":"journal-article","article-title":"The gastrointestinal tract and Parkinson's disease","volume":"13","author":"Higinbotham","year":"2023","unstructured":"Higinbotham, A. S., Kilbane, C. W. The gastrointestinal tract and Parkinson's disease. Front Cell Infect Microbiol. 13, 1158986 (2023).","journal-title":"Front Cell Infect Microbiol"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"225","DOI":"10.3748\/wjg.v30.i3.225","type":"journal-article","article-title":"Relationship among Parkinson's disease, constipation, microbes, and microbiological therapy","volume":"30","author":"Yuan","year":"2024","unstructured":"Yuan, X. Y., Chen, Y. S., Liu, Z. Relationship among Parkinson's disease, constipation, microbes, and microbiological therapy. World J Gastroenterol. 30 (3), 225-237 (2024).","journal-title":"World J Gastroenterol"},{"issue":"199","key":"ref6","first-page":"e65966","type":"journal-article","article-title":"Measuring constipation in a Drosophila model of Parkinson's disease","author":"Sodders","year":"2023","unstructured":"Sodders, M. J., Shen, M., Olsen, A. L. Measuring constipation in a Drosophila model of Parkinson's disease. J Vis Exp. 199, e65966 (2023).","journal-title":"J Vis Exp"},{"issue":"6645","key":"ref7","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1038\/42166","type":"journal-article","article-title":"Alpha-synuclein in Lewy bodies","volume":"388","author":"Spillantini","year":"1997","unstructured":"Spillantini, M. G. et al. Alpha-synuclein in Lewy bodies. Nature. 388 (6645), 839-840 (1997).","journal-title":"Nature"},{"issue":"12","key":"ref8","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1002\/mds.27527","type":"journal-article","article-title":"Genotype-phenotype relations for the Parkinson's disease genes SNCA, LRRK2, VPS35: MDSGene systematic review","volume":"33","author":"Trinh","year":"2018","unstructured":"Trinh, J. et al. Genotype-phenotype relations for the Parkinson's disease genes SNCA, LRRK2, VPS35: MDSGene systematic review. Mov Disord. 33 (12), 1857-1870 (2018).","journal-title":"Mov Disord"},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1002\/mds.27352","type":"journal-article","article-title":"Genotype-phenotype relations for the Parkinson's disease genes Parkin, PINK1, DJ1: MDSGene systematic review","volume":"33","author":"Kasten","year":"2018","unstructured":"Kasten, M. et al. Genotype-phenotype relations for the Parkinson's disease genes Parkin, PINK1, DJ1: MDSGene systematic review. Mov Disord. 33 (5), 733-741 (2018).","journal-title":"Mov Disord"},{"key":"ref10","type":"journal-article","article-title":"VPS35-related Parkinson disease","author":"Deutschl\u00e4nder","year":"2023","unstructured":"Deutschl\u00e4nder, A., Ross, O. A., Wszolek, Z. K. VPS35-related Parkinson disease. GeneReviews. at https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK447258\/ (2023).","journal-title":"GeneReviews"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.neures.2020.09.001","type":"journal-article","article-title":"Parkin-linked Parkinson's disease: From clinical insights to pathogenic mechanisms and novel therapeutic approaches","volume":"159","author":"Wasner","year":"2020","unstructured":"Wasner, K., Gr\u00fcnewald, A., Klein, C. Parkin-linked Parkinson's disease: From clinical insights to pathogenic mechanisms and novel therapeutic approaches. Neurosci Res. 159, 34-39 (2020).","journal-title":"Neurosci Res"},{"issue":"3","key":"ref12","doi-asserted-by":"crossref","first-page":"579","DOI":"10.3390\/cells10030579","type":"journal-article","article-title":"The importance of Drosophila melanogaster research to uncover cellular pathways underlying Parkinson's disease","volume":"10","author":"Vos","year":"2021","unstructured":"Vos, M., Klein, C. The importance of Drosophila melanogaster research to uncover cellular pathways underlying Parkinson's disease. Cells. 10 (3), 579 (2021).","journal-title":"Cells"},{"issue":"6776","key":"ref13","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1038\/35006074","type":"journal-article","article-title":"A Drosophila model of Parkinson's disease","volume":"404","author":"Feany","year":"2000","unstructured":"Feany, M. B., Bender, W. W. A Drosophila model of Parkinson's disease. Nature. 404 (6776), 394-398 (2000).","journal-title":"Nature"},{"issue":"7097","key":"ref14","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1038\/nature04788","type":"journal-article","article-title":"Mitochondrial dysfunction in Drosophila PINK1 mutants is complemented by parkin","volume":"441","author":"Park","year":"2006","unstructured":"Park, J. et al. Mitochondrial dysfunction in Drosophila PINK1 mutants is complemented by parkin. Nature. 441 (7097), 1157-1161 (2006).","journal-title":"Nature"},{"issue":"1","key":"ref15","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1038\/s41531-023-00584-z","type":"journal-article","article-title":"Characterization of the pathogenic \u03b1-synuclein variant V15A in Parkinson's disease","volume":"9","author":"Diaw","year":"2023","unstructured":"Diaw, S. H. et al. Characterization of the pathogenic \u03b1-synuclein variant V15A in Parkinson's disease. NPJ Parkinsons Dis. 9 (1), 148 (2023).","journal-title":"NPJ Parkinsons Dis"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1093\/beheco\/ark016","type":"journal-article","article-title":"The unequal variance t-test is an underused alternative to Student's t-test and the Mann-Whitney U test","volume":"17","author":"Ruxton","year":"2006","unstructured":"Ruxton, G. D. The unequal variance t-test is an underused alternative to Student's t-test and the Mann-Whitney U test. Behav Ecol. 17 (4), 688-690 (2006).","journal-title":"Behav Ecol"},{"issue":"4","key":"ref17","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/j.cmet.2018.05.019","type":"journal-article","article-title":"Phospholipase PLA2G6, a Parkinsonism-associated gene, affects Vps26 and Vps35, retromer function, and ceramide levels, similar to \u03b1-synuclein gain","volume":"28","author":"Lin","year":"2018","unstructured":"Lin, G. et al. Phospholipase PLA2G6, a Parkinsonism-associated gene, affects Vps26 and Vps35, retromer function, and ceramide levels, similar to \u03b1-synuclein gain. Cell Metab. 28 (4), 605-618.e6 (2018).","journal-title":"Cell Metab"},{"issue":"21","key":"ref18","first-page":"ijms222111998","type":"journal-article","article-title":"Neurodegenerative disorders: Spotlight on sphingolipids","volume":"5","author":"Mandik","year":"2021","unstructured":"Mandik, F., Vos, M. Neurodegenerative disorders: Spotlight on sphingolipids. Int J Mol Sci. 5 (21), ijms222111998 (2021).","journal-title":"Int J Mol Sci"},{"issue":"12","key":"ref19","doi-asserted-by":"crossref","first-page":"168000","DOI":"10.1016\/j.jmb.2023.168000","type":"journal-article","article-title":"Role of ceramides and sphingolipids in Parkinson's disease","volume":"435","author":"Vos","year":"2023","unstructured":"Vos, M., Klein, C., Hicks, A. A. Role of ceramides and sphingolipids in Parkinson's disease. J Mol Biol. 435 (12), 168000 (2023).","journal-title":"J Mol Biol"},{"issue":"7097","key":"ref20","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1038\/nature04779","type":"journal-article","article-title":"Drosophila pink1 is required for mitochondrial function and interacts genetically with parkin","volume":"441","author":"Clark","year":"2006","unstructured":"Clark, I. E. et al. Drosophila pink1 is required for mitochondrial function and interacts genetically with parkin. Nature. 441 (7097), 1162-1166 (2006).","journal-title":"Nature"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1126\/science.1218632","type":"journal-article","article-title":"Vitamin K2 is a mitochondrial electron carrier that rescues pink1 deficiency","volume":"336","author":"Vos","year":"2012","unstructured":"Vos, M. et al. Vitamin K2 is a mitochondrial electron carrier that rescues pink1 deficiency. Science. 336, 1306-1310 (2012).","journal-title":"Science"},{"issue":"4","key":"ref22","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1038\/s43587-022-00194-z","type":"journal-article","article-title":"Suppression of intestinal dysfunction in a Drosophila model of Parkinson's disease is neuroprotective","volume":"2","author":"Fedele","year":"2022","unstructured":"Fedele, G. et al. Suppression of intestinal dysfunction in a Drosophila model of Parkinson's disease is neuroprotective. Nat Aging. 2 (4), 317-331 (2022).","journal-title":"Nat Aging"},{"issue":"3","key":"ref23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41419-023-05729-9","type":"journal-article","article-title":"Blocking dPerk in the intestine suppresses neurodegeneration in a Drosophila model of Parkinson's disease","volume":"14","author":"Popovic","year":"2023","unstructured":"Popovic, R. et al. Blocking dPerk in the intestine suppresses neurodegeneration in a Drosophila model of Parkinson's disease. Cell Death Dis. 14 (3), 1-9 (2023).","journal-title":"Cell Death Dis"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.jinsphys.2014.05.023","type":"journal-article","article-title":"Spotting the differences: Probing host\/microbiota interactions with a dedicated software tool for the analysis of faecal outputs in Drosophila","volume":"69","author":"Wayland","year":"2014","unstructured":"Wayland, M. T. et al. Spotting the differences: Probing host\/microbiota interactions with a dedicated software tool for the analysis of faecal outputs in Drosophila. J Insect Physiol. 69, 126-135 (2014).","journal-title":"J Insect Physiol"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-023-45929-z","type":"journal-article","article-title":"Gut inflammation associated with age and Alzheimer's disease pathology: A human cohort study","volume":"13","author":"Heston","year":"2023","unstructured":"Heston, M. B. et al. Gut inflammation associated with age and Alzheimer's disease pathology: A human cohort study. Sci Rep. 13 (1), 1-12 (2023).","journal-title":"Sci Rep"},{"key":"ref26","first-page":"319201","type":"journal-article","article-title":"Adding to the burden: Gastrointestinal symptoms and syndromes in multiple sclerosis","volume":"2013","author":"Levinthal","year":"2013","unstructured":"Levinthal, D. J. et al. Adding to the burden: Gastrointestinal symptoms and syndromes in multiple sclerosis. Mult Scler Int. 2013, 319201 (2013).","journal-title":"Mult Scler Int"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"e0033820","DOI":"10.1128\/CMR.00338-20","type":"journal-article","article-title":"A comprehensive review on the role of the gut microbiome in human neurological disorders","volume":"35","author":"Sorboni","year":"2022","unstructured":"Sorboni, S. G. et al. A comprehensive review on the role of the gut microbiome in human neurological disorders. Clin Microbiol Rev. 35 (1), e0033820 (2022).","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"ref28","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s11689-021-09378-x","type":"journal-article","article-title":"The gut-microbiota-brain axis in autism: What Drosophila models can offer","volume":"13","author":"Salim","year":"2021","unstructured":"Salim, S. et al. The gut-microbiota-brain axis in autism: What Drosophila models can offer? J Neurodev Disord. 13 (1), 37 (2021).","journal-title":"J Neurodev Disord"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/68325\/gut-transit-defects-drosophila-models-monogenic-parkinson-s-disease","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:48:36Z","timestamp":1792594116000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/68325\/gut-transit-defects-drosophila-models-monogenic-parkinson-s-disease"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,11]]},"references-count":28,"journal-issue":{"issue":"221","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/68325","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,11]]}}}