{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:45:19Z","timestamp":1792597519288,"version":"4.5.0"},"reference-count":20,"publisher":"MyJove Corporation","issue":"221","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/68534","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T19:02:34Z","timestamp":1751569354000},"source":"Crossref","is-referenced-by-count":1,"title":["<em>In Vivo<\/em> Confocal Microscopy: A Standard Operating Procedure for The Detection of <em>Demodex<\/em> Mites at The Eyelid Margin"],"prefix":"10.3791","author":[{"given":"Tingting","family":"Yan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanshun","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"issue":"4","key":"ref1","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1097\/ICU.0000000000000168","type":"journal-article","article-title":"Recent advances on ocular Demodex infestation","volume":"26","author":"Cheng","year":"2015","unstructured":"Cheng, A. M., Sheha, H., Tseng, S. C. Recent advances on ocular Demodex infestation. Curr Opin Ophthalmol. 26 (4), 295-300 (2015).","journal-title":"Curr Opin Ophthalmol"},{"issue":"1","key":"ref2","first-page":"63","type":"journal-article","article-title":"Demodex folliculorum and Demodex brevis as a cause of chronic marginal blepharitis","volume":"53","author":"Czepita","year":"2007","unstructured":"Czepita, D., Ku\u017ana-Grygiel, W., Czepita, M., Grobelny, A. Demodex folliculorum and Demodex brevis as a cause of chronic marginal blepharitis. Ann Acad Med Stetin. 53 (1), 63-67; discussion 67 (2007).","journal-title":"Ann Acad Med Stetin"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1093\/jmedent\/27.4.467","type":"journal-article","article-title":"Association of diabetes, lash loss, and Staphylococcus aureus with infestation of eyelids by Demodex folliculorum (acari: Demodicidae)","volume":"27","author":"Clifford","year":"1990","unstructured":"Clifford, C. W. Fulk, G. W. Association of diabetes, lash loss, and Staphylococcus aureus with infestation of eyelids by Demodex folliculorum (acari: Demodicidae). J Med Entomol. 27 (4), 467-470 (1990).","journal-title":"J Med Entomol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"2979","DOI":"10.2147\/OPTH.S374530","type":"journal-article","article-title":"Psychosocial impact of Demodex blepharitis","volume":"16","author":"O'Dell","year":"2022","unstructured":"O'Dell, L. et al. Psychosocial impact of Demodex blepharitis. Clin Ophthalmol. 16, 2979-2987 (2022).","journal-title":"Clin Ophthalmol"},{"issue":"8","key":"ref5","first-page":"1261","type":"journal-article","article-title":"Psychosocial impact of Demodex blepharitis","volume":"62","author":"Yeu","year":"2021","unstructured":"Yeu, E., Holdbrook, M. J., Baba, S. N., Gomes, P. J. Psychosocial impact of Demodex blepharitis. Invest Ophthalmol Vis Sci. 62 (8), 1261-1261 (2021).","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/s12886-019-1074-5","type":"journal-article","article-title":"Evaluation of the ocular surface characteristics and Demodex infestation in paediatric and adult blepharokeratoconjunctivitis","volume":"19","author":"Wu","year":"2019","unstructured":"Wu, M., Wang, X., Han, J., Shao, T., Wang, Y. Evaluation of the ocular surface characteristics and Demodex infestation in paediatric and adult blepharokeratoconjunctivitis. BMC Ophthalmol. 19 (1), 67 (2019).","journal-title":"BMC Ophthalmol"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/s13071-024-06122-x","type":"journal-article","article-title":"Characteristics of bacterial community in eyelashes of patients with Demodex blepharitis","volume":"17","author":"Zou","year":"2024","unstructured":"Zou, D. et al. Characteristics of bacterial community in eyelashes of patients with Demodex blepharitis. Parasit Vectors. 17 (1), 64 (2024).","journal-title":"Parasit Vectors"},{"key":"ref8","first-page":"361","type":"journal-article","article-title":"Demodex folliculorum blepharitis","volume":"65","author":"Coston","year":"1967","unstructured":"Coston, T. O. Demodex folliculorum blepharitis. Trans Am Ophthalmol Soc. 65, 361-392 (1967).","journal-title":"Trans Am Ophthalmol Soc"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"e172","DOI":"10.1097\/OPX.0b013e318294c2c0","type":"journal-article","article-title":"Method to identify Demodex in the eyelash follicle without epilation","volume":"90","author":"Mastrota","year":"2013","unstructured":"Mastrota, K. M. Method to identify Demodex in the eyelash follicle without epilation. Optom Vis Sci. 90 (6), e172-174 (2013).","journal-title":"Optom Vis Sci"},{"issue":"9","key":"ref10","doi-asserted-by":"crossref","first-page":"3089","DOI":"10.1167\/iovs.05-0275","type":"journal-article","article-title":"High prevalence of demodex in eyelashes with cylindrical dandruff","volume":"46","author":"Gao","year":"2005","unstructured":"Gao, Y. Y. et al. High prevalence of demodex in eyelashes with cylindrical dandruff. Invest Ophthalmol Vis Sci. 46 (9), 3089-3094 (2005).","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"3","key":"ref11","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1136\/bjophthalmol-2014-305671","type":"journal-article","article-title":"In vivo confocal microscopy as a novel and reliable tool for the diagnosis of Demodex eyelid infestation","volume":"99","author":"Randon","year":"2015","unstructured":"Randon, M. et al. In vivo confocal microscopy as a novel and reliable tool for the diagnosis of Demodex eyelid infestation. Br J Ophthalmol. 99 (3), 336-341 (2015).","journal-title":"Br J Ophthalmol"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.clae.2019.11.009","type":"journal-article","article-title":"Improved Demodex diagnosis in the clinical setting using a novel in situ technique","volume":"43","author":"Muntz","year":"2020","unstructured":"Muntz, A., Purslow, C., Wolffsohn, J. S., Craig, J. P. Improved Demodex diagnosis in the clinical setting using a novel in situ technique. Cont Lens Anterior Eye. 43 (4), 345-349 (2020).","journal-title":"Cont Lens Anterior Eye"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1167\/iovs.10-5477","type":"journal-article","article-title":"In vivo evaluation of ocular demodicosis using laser scanning confocal microscopy","volume":"52","author":"Kojima","year":"2011","unstructured":"Kojima, T. et al. In vivo evaluation of ocular demodicosis using laser scanning confocal microscopy. Invest Ophthalmol Vis Sci. 52 (1), 565-569 (2011).","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"3","key":"ref14","first-page":"173","type":"journal-article","article-title":"Interobserver and intraobserver agreements of the detection of Demodex infestation by in vivo confocal microscopy","volume":"7","author":"Tas","year":"2022","unstructured":"Tas, A. Y. Interobserver and intraobserver agreements of the detection of Demodex infestation by in vivo confocal microscopy. Beyoglu Eye J. 7 (3), 173-180 (2022).","journal-title":"Beyoglu Eye J"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.ajo.2019.02.026","type":"journal-article","article-title":"Prospective study of the diagnostic accuracy of the in vivo laser scanning confocal microscopy for ocular demodicosis","volume":"203","author":"Wang","year":"2019","unstructured":"Wang, Y.-J., Ke, M., Chen, X.-M. Prospective study of the diagnostic accuracy of the in vivo laser scanning confocal microscopy for ocular demodicosis. Am J Ophthalmol. 203, 46-52 (2019).","journal-title":"Am J Ophthalmol"},{"key":"ref16","volume-title":"hrt3 rostock cornea module user manual v. 1.5 english","year":"2025","unstructured":"Heidelberg Engineering GmbH. hrt3 rostock cornea module user manual v. 1.5 english. http:\/\/www.HeidelbergEngineering.com (2025)."},{"issue":"6","key":"ref17","doi-asserted-by":"crossref","first-page":"2906","DOI":"10.1167\/iovs.09-4850","type":"journal-article","article-title":"The relationship between Demodex and ocular discomfort","volume":"51","author":"Lee","year":"2010","unstructured":"Lee, S. H., Chun, Y. S., Kim, J. H., Kim, E. S., Kim, J. C. The relationship between Demodex and ocular discomfort. Invest Ophthalmol Vis Sci. 51 (6), 2906-2911 (2010).","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"5","key":"ref18","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1111\/j.1365-2133.2012.11096.x","type":"journal-article","article-title":"Noninvasive in vivo detection and quantification of Demodex mites by confocal laser scanning microscopy","volume":"167","author":"Sattler","year":"2012","unstructured":"Sattler, E. C. et al. Noninvasive in vivo detection and quantification of Demodex mites by confocal laser scanning microscopy. Br J Dermatol. 167 (5), 1042-1047 (2012).","journal-title":"Br J Dermatol"},{"issue":"4","key":"ref19","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s40135-022-00300-9","type":"journal-article","article-title":"Demodex blepharitis: Its role and treatment in ocular surface disease","volume":"10","author":"Duncan","year":"2022","unstructured":"Duncan, N., Sood, P., Khandelwal, S. Demodex blepharitis: Its role and treatment in ocular surface disease. Curr Ophthalmol Rep. 10 (4), 107-113 (2022).","journal-title":"Curr Ophthalmol Rep"},{"issue":"Suppl 1","key":"ref20","doi-asserted-by":"crossref","first-page":"S9","DOI":"10.1097\/ICO.0000000000001361","type":"journal-article","article-title":"Ocular demodicosis as a potential cause of ocular surface inflammation","volume":"36 Suppl 1","author":"Luo","year":"2017","unstructured":"Luo, X., Li, J., Chen, C., Tseng, S., Liang, L. Ocular demodicosis as a potential cause of ocular surface inflammation. Cornea. 36 Suppl 1 (Suppl 1), S9-S14 (2017).","journal-title":"Cornea"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/68534\/in-vivo-confocal-microscopy-standard-operating-procedure-for","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:51:05Z","timestamp":1792594265000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/68534\/in-vivo-confocal-microscopy-standard-operating-procedure-for"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,3]]},"references-count":20,"journal-issue":{"issue":"221","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/68534","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,3]]}}}