{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:44:12Z","timestamp":1792593852142,"version":"4.5.0"},"reference-count":22,"publisher":"MyJove Corporation","issue":"224","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/68694","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:01:57Z","timestamp":1760137317000},"source":"Crossref","is-referenced-by-count":2,"title":["An Orthotopic Sciatic Nerve Xenograft for Neurofibromatosis Type 1 Neurofibromas"],"prefix":"10.3791","author":[{"given":"Manuela","family":"Ye","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lien","family":"Bore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Richon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Dangles-Marie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Pasmant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rapha\u00ebl","family":"Margueron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikael","family":"Hivelin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,10,10]]},"reference":[{"issue":"5","key":"ref1","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/S1470-2045(09)70033-6","type":"journal-article","article-title":"Mechanisms in the pathogenesis of malignant tumours in neurofibromatosis type 1","volume":"10","author":"Brems","year":"2009","unstructured":"Brems, H., Beert, E., De Ravel, T., Legius, E. Mechanisms in the pathogenesis of malignant tumours in neurofibromatosis type 1. Lancet Oncol. 10 (5), 508-515 (2009).","journal-title":"Lancet Oncol"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1093\/oxfordjournals.aje.a010118","type":"journal-article","article-title":"NF1 gene and neurofibromatosis 1","volume":"151","author":"Rasmussen","year":"2000","unstructured":"Rasmussen, S. A., Friedman, J. M. NF1 gene and neurofibromatosis 1. Am J Epidemiol. 151 (1), 33-40 (2000).","journal-title":"Am J Epidemiol"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"17004","DOI":"10.1038\/nrdp.2017.4","type":"journal-article","article-title":"Neurofibromatosis type 1","volume":"3","author":"Gutmann","year":"2017","unstructured":"Gutmann, D. H. et al. Neurofibromatosis type 1. Nat Rev Dis Primers. 3(1), 17004 (2017).","journal-title":"Nat Rev Dis Primers"},{"issue":"7","key":"ref4","doi-asserted-by":"crossref","first-page":"110385","DOI":"10.1016\/j.celrep.2022.110385","type":"journal-article","article-title":"Modeling iPSC-derived human neurofibroma-like tumors in mice uncovers the heterogeneity of Schwann cells within plexiform neurofibromas","volume":"38","author":"Mazuelas","year":"2022","unstructured":"Mazuelas, H. et al. Modeling iPSC-derived human neurofibroma-like tumors in mice uncovers the heterogeneity of Schwann cells within plexiform neurofibromas. Cell Rep. 38 (7), 110385 (2022).","journal-title":"Cell Rep"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/ng0794-353","type":"journal-article","article-title":"Tumour predisposition in mice heterozygous for a targeted mutation in Nf1","volume":"7","author":"Jacks","year":"1994","unstructured":"Jacks, T. et al. Tumour predisposition in mice heterozygous for a targeted mutation in Nf1. Nat Genet. 7 (3), 353-361 (1994).","journal-title":"Nat Genet"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1158\/2159-8290.CD-18-0156","type":"journal-article","article-title":"Cellular origin, tumor progression, and pathogenic mechanisms of cutaneous neurofibromas revealed by mice with Nf1 knockout in boundary cap cells","volume":"9","author":"Radomska","year":"2019","unstructured":"Radomska, K. J. et al. Cellular origin, tumor progression, and pathogenic mechanisms of cutaneous neurofibromas revealed by mice with Nf1 knockout in boundary cap cells. Cancer Discov. 9 (1), 130-147 (2019).","journal-title":"Cancer Discov"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1158\/2159-8290.CD-18-0151","type":"journal-article","article-title":"Spatiotemporal loss of NF1 in Schwann cell lineage leads to different types of cutaneous neurofibroma susceptible to modification by the Hippo pathway","volume":"9","author":"Chen","year":"2019","unstructured":"Chen, Z. et al. Spatiotemporal loss of NF1 in Schwann cell lineage leads to different types of cutaneous neurofibroma susceptible to modification by the Hippo pathway. Cancer Discov. 9 (1), 114-129 (2019).","journal-title":"Cancer Discov"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.ccr.2007.12.027","type":"journal-article","article-title":"Plexiform and dermal neurofibromas and pigmentation are caused by Nf1 loss in desert hedgehog-expressing cells","volume":"13","author":"Wu","year":"2008","unstructured":"Wu, J. et al. Plexiform and dermal neurofibromas and pigmentation are caused by Nf1 loss in desert hedgehog-expressing cells. Cancer Cell. 13 (2), 105-116 (2008).","journal-title":"Cancer Cell"},{"issue":"Suppl 1","key":"ref9","first-page":"i13","type":"journal-article","article-title":"New insights into the neurofibroma tumor cells of origin","volume":"2","author":"Li","year":"2020","unstructured":"Li, S., Chen, Z., Le, L. Q. New insights into the neurofibroma tumor cells of origin. Neurooncol Adv. 2 (Suppl 1), i13-i22 (2020).","journal-title":"Neurooncol Adv"},{"issue":"49","key":"ref10","first-page":"e2558","type":"journal-article","article-title":"Orthotopic xenografting of human luciferase-tagged malignant peripheral nerve sheath tumor cells for in vivo testing of candidate therapeutic agents","author":"Turk","year":"2011","unstructured":"Turk, A. N., Byer, S. J., Zinn, K. R., Carroll, S. L. Orthotopic xenografting of human luciferase-tagged malignant peripheral nerve sheath tumor cells for in vivo testing of candidate therapeutic agents. J Vis Exp. 49, e2558 (2011).","journal-title":"J Vis Exp"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1172\/JCI60578","type":"journal-article","article-title":"MEK inhibition exhibits efficacy in human and mouse neurofibromatosis tumors","volume":"123","author":"Jessen","year":"2013","unstructured":"Jessen, W. J. et al. MEK inhibition exhibits efficacy in human and mouse neurofibromatosis tumors. J Clin Invest. 123 (1), 340-347 (2013).","journal-title":"J Clin Invest"},{"issue":"10","key":"ref12","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1002\/pbc.25546","type":"journal-article","article-title":"Preclinical assessments of the MEK inhibitor PD-0325901 in a mouse model of neurofibromatosis type 1: MEK inhibition in neurofibroma","volume":"62","author":"Jousma","year":"2015","unstructured":"Jousma, E. et al. Preclinical assessments of the MEK inhibitor PD-0325901 in a mouse model of neurofibromatosis type 1: MEK inhibition in neurofibroma. Pediatr Blood Cancer. 62 (10), 1709-1716 (2015).","journal-title":"Pediatr Blood Cancer"},{"key":"ref13","first-page":"153","type":"journal-article","article-title":"PDX models for functional precision oncology and discovery science","volume":"25","author":"Blanchard","year":"2024","unstructured":"Blanchard, Z., Brown, E. A., Ghazaryan, A., Welm, A. L. PDX models for functional precision oncology and discovery science. Nat Rev Cancer. 25, 153-166 (2024).","journal-title":"Nat Rev Cancer"},{"issue":"10","key":"ref14","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1038\/labinvest.2016.88","type":"journal-article","article-title":"Immortalization of human normal and NF1 neurofibroma Schwann cells","volume":"96","author":"Li","year":"2016","unstructured":"Li, H., Chang, L. J., Neubauer, D. R., Muir, D. F., Wallace, M. R. Immortalization of human normal and NF1 neurofibroma Schwann cells. Lab Invest. 96 (10), 1105-1115 (2016).","journal-title":"Lab Invest"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/S0002-9440(10)63992-2","type":"journal-article","article-title":"Tumorigenic properties of neurofibromin-deficient neurofibroma Schwann cells","volume":"158","author":"Muir","year":"2001","unstructured":"Muir, D., Neubauer, D., Lim, I. T., Yachnis, A. T., Wallace, M. R. Tumorigenic properties of neurofibromin-deficient neurofibroma Schwann cells. Am J Pathol. 158 (2), 501-513 (2001).","journal-title":"Am J Pathol"},{"issue":"3","key":"ref16","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1002\/jnr.20646","type":"journal-article","article-title":"Tumorigenic properties of neurofibromin-deficient Schwann cells in culture and as syngrafts in Nf1 knockout mice","volume":"82","author":"Wu","year":"2005","unstructured":"Wu, M., Wallace, M. R., Muir, D. Tumorigenic properties of neurofibromin-deficient Schwann cells in culture and as syngrafts in Nf1 knockout mice. J Neurosci Res. 82 (3), 357-367 (2005).","journal-title":"J Neurosci Res"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1093\/oxfordjournals.aje.a010118","type":"journal-article","article-title":"NF1 gene and neurofibromatosis 1","volume":"151","author":"Rasmussen","year":"2000","unstructured":"Rasmussen, S. A., Friedman, J. M. NF1 gene and neurofibromatosis 1. Am J Epidemiol. 151 (1), 33-40 (2000).","journal-title":"Am J Epidemiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.humpath.2017.05.010","type":"journal-article","article-title":"Histopathologic evaluation of atypical neurofibromatous tumors and their transformation into malignant peripheral nerve sheath tumor in patients with neurofibromatosis 1: A consensus overview","volume":"67","author":"Miettinen","year":"2017","unstructured":"Miettinen, M. M. et al. Histopathologic evaluation of atypical neurofibromatous tumors and their transformation into malignant peripheral nerve sheath tumor in patients with neurofibromatosis 1: A consensus overview. Hum Pathol. 67, 1-10 (2017).","journal-title":"Hum Pathol"},{"issue":"42","key":"ref19","doi-asserted-by":"crossref","first-page":"eado6342","DOI":"10.1126\/sciadv.ado6342","type":"journal-article","article-title":"Stimulator of interferon gene facilitates recruitment of effector CD8 T cells that drive neurofibromatosis type 1 nerve tumor initiation and maintenance","volume":"10","author":"Pundavela","year":"2024","unstructured":"Pundavela, J. et al. Stimulator of interferon gene facilitates recruitment of effector CD8 T cells that drive neurofibromatosis type 1 nerve tumor initiation and maintenance. Sci Adv. 10 (42), eado6342 (2024).","journal-title":"Sci Adv"},{"issue":"Suppl 1","key":"ref20","first-page":"i23","type":"journal-article","article-title":"After Nf1 loss in Schwann cells, inflammation drives neurofibroma formation","volume":"2","author":"Fletcher","year":"2020","unstructured":"Fletcher, J. S., Pundavela, J., Ratner, N. After Nf1 loss in Schwann cells, inflammation drives neurofibroma formation. Neurooncol Adv. 2 (Suppl 1), i23-i32 (2020).","journal-title":"Neurooncol Adv"},{"issue":"32","key":"ref21","doi-asserted-by":"crossref","first-page":"4609","DOI":"10.1038\/sj.onc.1210261","type":"journal-article","article-title":"Tumor microenvironment and neurofibromatosis type I: Connecting the GAPs","volume":"26","author":"Le","year":"2007","unstructured":"Le, L. Q., Parada, L. F. Tumor microenvironment and neurofibromatosis type I: Connecting the GAPs. Oncogene. 26 (32), 4609-4616 (2007).","journal-title":"Oncogene"},{"issue":"4","key":"ref22","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1006\/mthe.2001.0460","type":"journal-article","article-title":"Non-invasive optical imaging of firefly luciferase reporter gene expression in skeletal muscles of living mice","volume":"4","author":"Wu","year":"2001","unstructured":"Wu, J. C., Sundaresan, G., Iyer, M., Gambhir, S. S. Non-invasive optical imaging of firefly luciferase reporter gene expression in skeletal muscles of living mice. Mol Ther. 4 (4), 297-306 (2001).","journal-title":"Mol Ther"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/68694\/an-orthotopic-sciatic-nerve-xenograft-for-neurofibromatosis-type-1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:24:18Z","timestamp":1792592658000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/68694\/an-orthotopic-sciatic-nerve-xenograft-for-neurofibromatosis-type-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,10]]},"references-count":22,"journal-issue":{"issue":"224","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/68694","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,10]]}}}