{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:40:15Z","timestamp":1792593615464,"version":"4.5.0"},"reference-count":39,"publisher":"MyJove Corporation","issue":"227","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/68724","type":"journal-article","created":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T00:01:46Z","timestamp":1768608106000},"source":"Crossref","is-referenced-by-count":1,"title":["Mouse Sciatic Nerve Transection\/Resuture Procedure for Studying Nerve Repair and Functional Recovery"],"prefix":"10.3791","author":[{"given":"Su-Hyuk","family":"Ko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizhen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2026,1,16]]},"reference":[{"issue":"12","key":"ref1","doi-asserted-by":"crossref","first-page":"10123186","DOI":"10.3390\/biomedicines10123186","type":"journal-article","article-title":"Axonal Regeneration: Underlying molecular mechanisms and potential therapeutic targets","volume":"10","author":"Akram","year":"2022","unstructured":"Akram, R. et al. Axonal Regeneration: Underlying molecular mechanisms and potential therapeutic targets. Biomedicines. 10 (12), 10123186 (2022).","journal-title":"Biomedicines"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"190","DOI":"10.3389\/fcell.2020.00190","type":"journal-article","article-title":"The influence of neuron-extrinsic factors and aging on injury progression and axonal repair in the central nervous system","volume":"8","author":"Sutherland","year":"2020","unstructured":"Sutherland, T. C., Geoffroy, C. G. The influence of neuron-extrinsic factors and aging on injury progression and axonal repair in the central nervous system. Front Cell Dev Biol. 8 190 (2020).","journal-title":"Front Cell Dev Biol"},{"issue":"15","key":"ref3","doi-asserted-by":"crossref","first-page":"5744","DOI":"10.1523\/JNEUROSCI.6810-10.2011","type":"journal-article","article-title":"Reduced BACE1 activity enhances clearance of myelin debris and regeneration of axons in the injured peripheral nervous system","volume":"31","author":"Farah","year":"2011","unstructured":"Farah, M. H. et al. Reduced BACE1 activity enhances clearance of myelin debris and regeneration of axons in the injured peripheral nervous system. J Neurosci. 31 (15), 5744-5754 (2011).","journal-title":"J Neurosci"},{"issue":"6","key":"ref4","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/s41583-018-0001-8","type":"journal-article","article-title":"Intrinsic mechanisms of neuronal axon regeneration","volume":"19","author":"Mahar","year":"2018","unstructured":"Mahar, M., Cavalli, V. Intrinsic mechanisms of neuronal axon regeneration. Nat Rev Neurosci. 19 (6), 323-337 (2018).","journal-title":"Nat Rev Neurosci"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.neuron.2016.04.022","type":"journal-article","article-title":"Intrinsic control of axon regeneration","volume":"90","author":"He","year":"2016","unstructured":"He, Z., Jin, Y. Intrinsic control of axon regeneration. Neuron. 90 (3), 437-451 (2016).","journal-title":"Neuron"},{"issue":"12","key":"ref6","doi-asserted-by":"crossref","first-page":"4718","DOI":"10.1523\/JNEUROSCI.19-12-04718.1999","type":"journal-article","article-title":"Temperature modulation reveals three distinct stages of Wallerian degeneration","volume":"19","author":"Tsao","year":"1999","unstructured":"Tsao, J. W., George, E. B., Griffin, J. W. Temperature modulation reveals three distinct stages of Wallerian degeneration. J Neurosci. 19 (12), 4718-4726 (1999).","journal-title":"J Neurosci"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1146\/annurev.neuro.30.051606.094354","type":"journal-article","article-title":"Why is Wallerian degeneration in the CNS so slow","volume":"30","author":"Vargas","year":"2007","unstructured":"Vargas, M. E., Barres, B. A. Why is Wallerian degeneration in the CNS so slow? Annu Rev Neurosci. 30, 153-179 (2007).","journal-title":"Annu Rev Neurosci"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1186\/1742-2094-8-110","type":"journal-article","article-title":"Wallerian degeneration: Gaining perspective on inflammatory events after peripheral nerve injury","volume":"8","author":"Gaudet","year":"2011","unstructured":"Gaudet, A. D., Popovich, P. G., Ramer, M. S. Wallerian degeneration: Gaining perspective on inflammatory events after peripheral nerve injury. J Neuroinflammation. 8, 110 (2011).","journal-title":"J Neuroinflammation"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"116","DOI":"10.7150\/ijbs.35653","type":"journal-article","article-title":"Current status of therapeutic approaches against peripheral nerve injuries: A detailed story from injury to recovery","volume":"16","author":"Hussain","year":"2020","unstructured":"Hussain, G. et al. Current status of therapeutic approaches against peripheral nerve injuries: A detailed story from injury to recovery. Int J Biol Sci. 16 (1), 116-134 (2020).","journal-title":"Int J Biol Sci"},{"issue":"2","key":"ref10","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.brainresrev.2006.09.005","type":"journal-article","article-title":"The making of successful axonal regeneration: genes, molecules and signal transduction pathways","volume":"53","author":"Raivich","year":"2007","unstructured":"Raivich, G., Makwana, M. The making of successful axonal regeneration: genes, molecules and signal transduction pathways. Brain Res Rev. 53 (2), 287-311 (2007).","journal-title":"Brain Res Rev"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.pneurobio.2018.11.004","type":"journal-article","article-title":"Axonal fusion: An alternative and efficient mechanism of nerve repair","volume":"173","author":"Neumann","year":"2019","unstructured":"Neumann, B., Linton, C., Giordano-Santini, R., Hilliard, M. A. Axonal fusion: An alternative and efficient mechanism of nerve repair. Prog Neurobiol. 173, 88-101 (2019).","journal-title":"Prog Neurobiol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1126\/science.156.3772.251","type":"journal-article","article-title":"Regeneration in Crustacean Motoneurons: Evidence for axonal fusion","volume":"156","author":"Hoy","year":"1967","unstructured":"Hoy, R. R., Bittner, G. D., Kennedy, D. Regeneration in Crustacean Motoneurons: Evidence for axonal fusion. Science. 156, 251-252 (1967).","journal-title":"Science"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/0006-8993(76)90058-5","type":"journal-article","article-title":"Regeneration of giant axons in earthworms","volume":"113","author":"Birse","year":"1976","unstructured":"Birse, S. C., Bittner, G. D. Regeneration of giant axons in earthworms. Brain Res. 113, 575-581 (1976).","journal-title":"Brain Res"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/0006-8993(78)90512-7","type":"journal-article","article-title":"Specific reinnervation of a target organ by a pair of identified molluscan neurons","volume":"156","author":"Murphy","year":"1978","unstructured":"Murphy, A. D., Kater, S. B. Specific reinnervation of a target organ by a pair of identified molluscan neurons. Brain Res. 156, 322-328 (1978).","journal-title":"Brain Res"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/0006-8993(83)90373-6","type":"journal-article","article-title":"Morphological evidence that regenerating axons can fuse with severed axon segments","volume":"272","author":"Deriemer","year":"1983","unstructured":"Deriemer, S. A., Elliott, E. J., Macagno, E. R., Muller, K. J. Morphological evidence that regenerating axons can fuse with severed axon segments. Brain Res. 272, 157-161 (1983).","journal-title":"Brain Res"},{"issue":"24","key":"ref16","doi-asserted-by":"crossref","first-page":"9667","DOI":"10.1523\/JNEUROSCI.21-24-09667.2001","type":"journal-article","article-title":"Axonal rejoining inhibits injury-induced long-term changes in aplysia sensory neurons in vitro","volume":"21","author":"Bedi","year":"2001","unstructured":"Bedi, S. S., Glanzman, D. L. Axonal rejoining inhibits injury-induced long-term changes in aplysia sensory neurons in vitro. J Neurosci. 21 (24), 9667-9677 (2001).","journal-title":"J Neurosci"},{"issue":"6","key":"ref17","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1002\/dvdy.22606","type":"journal-article","article-title":"Axonal regeneration proceeds through specific axonal fusion in transected C. elegans neurons","volume":"240","author":"Neumann","year":"2011","unstructured":"Neumann, B., Nguyen, K. C., Hall, D. H., Ben-Yakar, A., Hilliard, M. A. Axonal regeneration proceeds through specific axonal fusion in transected C. elegans neurons. Dev Dyn. 240 (6), 1365-1372 (2011).","journal-title":"Dev Dyn"},{"issue":"5","key":"ref18","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1002\/jnr.23023","type":"journal-article","article-title":"effective, and long-lasting behavioral recovery produced by microsutures, methylene blue, and polyethylene glycol after completely cutting rat sciatic nerves","volume":"90","author":"Bittner","year":"2012","unstructured":"Bittner, G. D. et al. Rapid, effective, and long-lasting behavioral recovery produced by microsutures, methylene blue, and polyethylene glycol after completely cutting rat sciatic nerves. J Neurosci Res. 90 (5), 967-980 (2012).","journal-title":"J Neurosci Res"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"S177","DOI":"10.1097\/TA.0000000000001203","type":"journal-article","article-title":"A novel therapy to promote axonal fusion in human digital nerves","volume":"81","author":"Bamba","year":"2016","unstructured":"Bamba, R. et al. A novel therapy to promote axonal fusion in human digital nerves. J Trauma Acute Care Surg. 81, S177-S183 (2016).","journal-title":"J Trauma Acute Care Surg"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1242\/jeb.205.1.1","type":"journal-article","article-title":"Behavioral recovery from spinal cord injury following delayed application of polyethylene glycol","volume":"205","author":"Borgens","year":"2002","unstructured":"Borgens, R. B., Shi, R., Bohnert, D. Behavioral recovery from spinal cord injury following delayed application of polyethylene glycol. J Exp Biol. 205, 1-12 (2002).","journal-title":"J Exp Biol"},{"issue":"Suppl 24","key":"ref21","doi-asserted-by":"crossref","first-page":"S637","DOI":"10.4103\/2152-7806.190476","type":"journal-article","article-title":"Accelerated recovery of sensorimotor function in a dog submitted to quasi-total transection of the cervical spinal cord and treated with PEG","volume":"7","author":"Kim","year":"2016","unstructured":"Kim, C. Y. et al. Accelerated recovery of sensorimotor function in a dog submitted to quasi-total transection of the cervical spinal cord and treated with PEG. Surg Neurol Int. 7 (Suppl 24), S637-640 (2016).","journal-title":"Surg Neurol Int"},{"issue":"Suppl 24","key":"ref22","doi-asserted-by":"crossref","first-page":"S629","DOI":"10.4103\/2152-7806.190474","type":"journal-article","article-title":"GEMINI: Initial behavioral results after full severance of the cervical spinal cord in mice","volume":"7","author":"Kim","year":"2016","unstructured":"Kim, C. Y., Oh, H., Hwang, I. K., Hong, K. S. GEMINI: Initial behavioral results after full severance of the cervical spinal cord in mice. Surg Neurol Int. 7 (Suppl 24), S629-631 (2016).","journal-title":"Surg Neurol Int"},{"issue":"5","key":"ref23","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1016\/j.surg.2017.10.015","type":"journal-article","article-title":"Restoration of motor function after operative reconstruction of the acutely transected spinal cord in the canine model","volume":"163","author":"Liu","year":"2018","unstructured":"Liu, Z. et al. Restoration of motor function after operative reconstruction of the acutely transected spinal cord in the canine model. Surgery. 163 (5), 976-983 (2018).","journal-title":"Surgery"},{"issue":"5","key":"ref24","first-page":"2406","type":"journal-article","article-title":"Acute repair of crushed cuinea pig spinal cord by polyethylene glycol","volume":"81","author":"Shi","year":"1999","unstructured":"Shi, R., Borgens, R. B. Acute repair of crushed cuinea pig spinal cord by polyethylene glycol. Am Physiol Soc. 81 (5), 2406-2414 (1999).","journal-title":"Am Physiol Soc"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1038\/s41467-025-56382-z","type":"journal-article","article-title":"GPX modulation promotes regenerative axonal fusion and functional recovery after injury through PSR-1 condensation","volume":"16","author":"Ko","year":"2025","unstructured":"Ko, S. H. et al. GPX modulation promotes regenerative axonal fusion and functional recovery after injury through PSR-1 condensation. Nat Commun. 16 (1), 1079 (2025).","journal-title":"Nat Commun"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1002\/ar.1092150111","type":"journal-article","article-title":"Fiber composition of the rat sciatic nerve","volume":"215","author":"Schmalbruch","year":"1986","unstructured":"Schmalbruch, H. Fiber composition of the rat sciatic nerve. Anat Rec. 215 (1), 71-81 (1986).","journal-title":"Anat Rec"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/0014-4886(91)90087-S","type":"journal-article","article-title":"Sensory neurons of the rat sciatic nerve","volume":"114","author":"Swett","year":"1991","unstructured":"Swett, J. E., Torigoe, Y., Elie, V. R., Bourassa, C. M., Miller, P. G. Sensory neurons of the rat sciatic nerve. Exp Neurol. 114 (1), 82-103 (1991).","journal-title":"Exp Neurol"},{"issue":"1-2","key":"ref28","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.pain.2008.01.016","type":"journal-article","article-title":"Species and strain differences in rodent sciatic nerve anatomy: Implications for studies of neuropathic pain","volume":"136","author":"Rigaud","year":"2008","unstructured":"Rigaud, M. et al. Species and strain differences in rodent sciatic nerve anatomy: Implications for studies of neuropathic pain. Pain. 136 (1-2), 188-201 (2008).","journal-title":"Pain"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.jneumeth.2014.01.020","type":"journal-article","article-title":"Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery","volume":"227","author":"Savastano","year":"2014","unstructured":"Savastano, L. E. et al. Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery. J Neurosci Methods. 227, 166-180 (2014).","journal-title":"J Neurosci Methods"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.bbr.2010.12.018","type":"journal-article","article-title":"Aspects of static and dynamic motor function in peripheral nerve regeneration: SSI and CatWalk gait analysis","volume":"219","author":"Bozkurt","year":"2011","unstructured":"Bozkurt, A. et al. Aspects of static and dynamic motor function in peripheral nerve regeneration: SSI and CatWalk gait analysis. Behav Brain Res. 219 (1), 55-62 (2011).","journal-title":"Behav Brain Res"},{"issue":"1","key":"ref31","doi-asserted-by":"crossref","first-page":"21637","DOI":"10.1038\/s41598-020-78481-1","type":"journal-article","article-title":"A novel nerve transection and repair method in mice: histomorphometric analysis of nerves, blood vessels, and muscles with functional recovery","volume":"10","author":"Lee","year":"2020","unstructured":"Lee, J. I. et al. A novel nerve transection and repair method in mice: histomorphometric analysis of nerves, blood vessels, and muscles with functional recovery. Sci Rep. 10 (1), 21637 (2020).","journal-title":"Sci Rep"},{"issue":"Pt 2","key":"ref32","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1107\/S2059798320016125","type":"journal-article","article-title":"Crystal structures of the selenoprotein glutathione peroxidase 4 in its apo form and in complex with the covalently bound inhibitor ML162","volume":"77","author":"Moosmayer","year":"2021","unstructured":"Moosmayer, D. et al. Crystal structures of the selenoprotein glutathione peroxidase 4 in its apo form and in complex with the covalently bound inhibitor ML162. Acta Crystallogr D Struct Biol. 77 (Pt 2), 237-248 (2021).","journal-title":"Acta Crystallogr D Struct Biol"},{"issue":"11","key":"ref33","doi-asserted-by":"crossref","first-page":"4332","DOI":"10.1172\/JCI58675","type":"journal-article","article-title":"Accelerating axonal growth promotes motor recovery after peripheral nerve injury in mice","volume":"121","author":"Ma","year":"2011","unstructured":"Ma, C. H. et al. Accelerating axonal growth promotes motor recovery after peripheral nerve injury in mice. J Clin Invest. 121 (11), 4332-4347 (2011).","journal-title":"J Clin Invest"},{"issue":"2","key":"ref34","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1152\/jn.01051.2009","type":"journal-article","article-title":"Polyethylene glycol rapidly restores axonal integrity and improves the rate of motor behavior recovery after sciatic nerve crush injury","volume":"104","author":"Britt","year":"2010","unstructured":"Britt, J. M. et al. Polyethylene glycol rapidly restores axonal integrity and improves the rate of motor behavior recovery after sciatic nerve crush injury. J Neurophysiol. 104 (2), 695-703 (2010).","journal-title":"J Neurophysiol"},{"issue":"2","key":"ref35","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1177\/107385840000600207","type":"journal-article","article-title":"Degeneration, trophic interactions, and repair of severed axons: A reconsideration of some common assumptions","volume":"6","author":"Bittner","year":"2000","unstructured":"Bittner, G. D., Schallert, T., Peduzzi, J. D. Degeneration, trophic interactions, and repair of severed axons: A reconsideration of some common assumptions. Neuroscientist. 6 (2), 88-109 (2000).","journal-title":"Neuroscientist"},{"issue":"1-2","key":"ref36","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0006-8993(86)91617-3","type":"journal-article","article-title":"Reconnection of severed nerve axons with polyethylene glycol","volume":"367","author":"Bittner","year":"1986","unstructured":"Bittner, G. D., Ballinger, M. L., Raymond, M. A. Reconnection of severed nerve axons with polyethylene glycol. Brain Res. 367 (1-2), 351-355 (1986).","journal-title":"Brain Res"},{"issue":"5","key":"ref37","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1002\/jnr.23022","type":"journal-article","article-title":"Cellular mechanisms of plasmalemmal sealing and axonal repair by polyethylene glycol and methylene blue","volume":"90","author":"Spaeth","year":"2012","unstructured":"Spaeth, C. S., Robison, T., Fan, J. D., Bittner, G. D. Cellular mechanisms of plasmalemmal sealing and axonal repair by polyethylene glycol and methylene blue. J Neurosci Res. 90 (5), 955-966 (2012).","journal-title":"J Neurosci Res"},{"issue":"47","key":"ref38","doi-asserted-by":"crossref","first-page":"15790","DOI":"10.1523\/JNEUROSCI.4155-10.2010","type":"journal-article","article-title":"A model for sealing plasmalemmal damage in neurons and other eukaryotic cells","volume":"30","author":"Spaeth","year":"2010","unstructured":"Spaeth, C. S., Boydston, E. A., Figard, L. R., Zuzek, A., Bittner, G. D. A model for sealing plasmalemmal damage in neurons and other eukaryotic cells. J Neurosci. 30 (47), 15790-15800 (2010).","journal-title":"J Neurosci"},{"issue":"6","key":"ref39","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1002\/micr.22109","type":"journal-article","article-title":"Comparison between suture and fibrin glue on repair by direct coaptation or tubulization of injured mouse sciatic nerve","volume":"33","author":"F\u00e9lix","year":"2013","unstructured":"F\u00e9lix, S. P., Pereira Lopes, F. R., Marques, S. A., Martinez, A. M. Comparison between suture and fibrin glue on repair by direct coaptation or tubulization of injured mouse sciatic nerve. Microsurgery. 33 (6), 468-477 (2013).","journal-title":"Microsurgery"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/68724\/mouse-sciatic-nerve-transectionresuture-procedure-for-studying-nerve","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:52:43Z","timestamp":1792590763000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/68724\/mouse-sciatic-nerve-transectionresuture-procedure-for-studying-nerve"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,16]]},"references-count":39,"journal-issue":{"issue":"227","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.3791\/68724","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,16]]}}}