{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:44:36Z","timestamp":1792586676698,"version":"4.5.0"},"reference-count":15,"publisher":"MyJove Corporation","issue":"235","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/69006","type":"journal-article","created":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T23:08:57Z","timestamp":1789254537000},"source":"Crossref","is-referenced-by-count":0,"title":["Optimized Hot Phenol&amp;#8211;Based RNA Extraction from Mycobacteria: A Robust Approach for Reliable Gene Expression Analysis"],"prefix":"10.3791","author":[{"given":"Hritika","family":"Verma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rishav Kumar","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoj","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kajal","family":"Kanchan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"ref1","volume-title":"Global tuberculosis report, 2024","year":"2024","unstructured":"Global tuberculosis report. https:\/\/www.who.int\/publications\/b\/74877 (2024).","type":"book"},{"issue":"7","key":"ref2","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1111\/resp.13304","type":"journal-article","article-title":"Drug-resistant tuberculosis: An update on disease burden, diagnosis and treatment","volume":"23","author":"Lange","year":"2018","unstructured":"Lange, C. et al. Drug-resistant tuberculosis: An update on disease burden, diagnosis and treatment. Respirology. 23 (7), 656\u2013673 (2018).","journal-title":"Respirology"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"e1403","DOI":"10.1371\/journal.pone.0001403","type":"journal-article","article-title":"Probing host-pathogen cross-talk by transcriptional profiling of both Mycobacterium tuberculosis and infected human dendritic cells and macrophages","volume":"3","author":"Tailleux","year":"2008","unstructured":"Tailleux, L. et al. Probing host-pathogen cross-talk by transcriptional profiling of both Mycobacterium tuberculosis and infected human dendritic cells and macrophages. PLoS One. 3 (1), e1403 (2008).","journal-title":"PLoS One"},{"issue":"5","key":"ref4","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1084\/jem.20030205","type":"journal-article","article-title":"Inhibition of respiration by nitric oxide induces a Mycobacterium tuberculosis dormancy program","volume":"198","author":"Voskuil","year":"2003","unstructured":"Voskuil, M. I. et al. Inhibition of respiration by nitric oxide induces a Mycobacterium tuberculosis dormancy program. J Exp Med. 198 (5), 705\u2013713 (2003).","journal-title":"J Exp Med"},{"issue":"11","key":"ref5","first-page":"1320","type":"journal-article","article-title":"Mechanisms of drug resistance in Mycobacterium tuberculosis","volume":"13","author":"Zhang","year":"2009","unstructured":"Zhang, Y., Yew, W. W. Mechanisms of drug resistance in Mycobacterium tuberculosis. Int J Tuberc Lung Dis. 13 (11), 1320\u20131330 (2009).","journal-title":"Int J Tuberc Lung Dis"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1111\/j.1365-2958.2009.06777.x","type":"journal-article","article-title":"Identification of small RNAs in Mycobacterium tuberculosis","volume":"73","author":"Arnvig","year":"2009","unstructured":"Arnvig, K. B., Young, D. B. Identification of small RNAs in Mycobacterium tuberculosis. Mol Microbiol. 73 (3), 397\u2013408 (2009).","journal-title":"Mol Microbiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1146\/annurev.bi.64.070195.000333","type":"journal-article","article-title":"The envelope of mycobacteria","volume":"64","author":"Brennan","year":"1995","unstructured":"Brennan, P. J., Nikaido, H. The envelope of mycobacteria. Annu Rev Biochem. 64, 29\u201363 (1995).","journal-title":"Annu Rev Biochem"},{"key":"ref8","type":"book","article-title":"Isolation of genomic DNA from mycobacteria","volume-title":"Methods Mol Biol","author":"Belisle","year":"1998","unstructured":"Belisle, J. T., Sonnenberg, M. G. Isolation of genomic DNA from mycobacteria. Methods Mol Biol. 101, 31\u201344 (1998)."},{"issue":"2","key":"ref9","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1046\/j.1365-2958.2002.03005.x","type":"journal-article","article-title":"Role of the extracytoplasmic-function sigma factor \u03c3H in Mycobacterium tuberculosis global gene expression","volume":"45","author":"Manganelli","year":"2002","unstructured":"Manganelli, R. et al. Role of the extracytoplasmic-function sigma factor \u03c3H in Mycobacterium tuberculosis global gene expression. Mol Microbiol. 45 (2), 365\u2013374 (2002).","journal-title":"Mol Microbiol"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1093\/nar\/gks1019","type":"journal-article","article-title":"Global analysis of mRNA stability in Mycobacterium tuberculosis","volume":"41","author":"Rustad","year":"2013","unstructured":"Rustad, T. R. et al. Global analysis of mRNA stability in Mycobacterium tuberculosis. Nucleic Acids Res. 41 (1), 509\u2013517 (2013).","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"ref11","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1093\/nar\/25.3.675","type":"journal-article","article-title":"An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria","volume":"25","author":"Mangan","year":"1997","unstructured":"Mangan, J. A., Sole, K. M., Mitchson, D. A., Butcher, P. D. An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria. Nucleic Acids Res. 25 (3), 675\u2013676 (1997).","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"ref12","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.mimet.2013.03.018","type":"journal-article","article-title":"A robust and efficient method for the isolation of DNA-free, pure and intact RNA from Mycobacterium tuberculosis","volume":"93","author":"Venkataraman","year":"2013","unstructured":"Venkataraman, B., Gupta, N., Gupta, A. A robust and efficient method for the isolation of DNA-free, pure and intact RNA from Mycobacterium tuberculosis. J Microbiol Meth. 93 (3), 198\u2013202 (2013).","journal-title":"J Microbiol Meth"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1080\/07366205.2025.2457887","type":"journal-article","article-title":"RNA extraction and RNA-sequencing method for transcriptomic analysis of Mycobacterium tuberculosis","volume":"77","author":"Hiebert","year":"2025","unstructured":"Hiebert, R. M. et al. RNA extraction and RNA-sequencing method for transcriptomic analysis of Mycobacterium tuberculosis. Biotechniques. 77 (1), 23\u201334 (2025).","journal-title":"Biotechniques"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"e7615","DOI":"10.1371\/journal.pone.0007615","type":"journal-article","article-title":"Phenylalanine-rich peptides potently bind ESAT6, a virulence determinant of Mycobacterium tuberculosis, and concurrently affect the pathogen's growth","volume":"4","author":"Kumar","year":"2009","unstructured":"Kumar, K., Tharad, M., Ganapathy, S. et al, Phenylalanine-rich peptides potently bind ESAT6, a virulence determinant of Mycobacterium tuberculosis, and concurrently affect the pathogen's growth. PLoS One. 4, e7615 (2009).","journal-title":"PLoS One"},{"issue":"2","key":"ref15","first-page":"64","type":"journal-article","article-title":"Optimization of RNA extraction in Mycobacterium tuberculosis for studying intracellular gene expression","volume":"10","author":"Alli","year":"2009","unstructured":"Alli, A. O. et al. Optimization of RNA extraction in Mycobacterium tuberculosis for studying intracellular gene expression. Afr J Clin Exp Microbiol. 10 (2), 64\u201379 (2009).","journal-title":"Afr J Clin Exp Microbiol"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/69006\/optimized-hot-phenolbased-rna-extraction-from-mycobacteria-robust","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:13:39Z","timestamp":1792584819000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/69006\/optimized-hot-phenolbased-rna-extraction-from-mycobacteria-robust"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":15,"journal-issue":{"issue":"235","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.3791\/69006","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]}}}