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Aging Cell. 22 (11), e14004 (2023).","journal-title":"Aging Cell"},{"issue":"6","key":"ref50","doi-asserted-by":"crossref","first-page":"e27336","DOI":"10.1016\/j.heliyon.2024.e27336","type":"journal-article","article-title":"Follicular fluid aids cell adhesion, spreading in an age independent manner and shows an age-dependent effect on DNA damage in fallopian tube epithelial cells","volume":"10","author":"Salvi","year":"2024","unstructured":"Salvi, A. et al. Follicular fluid aids cell adhesion, spreading in an age independent manner and shows an age-dependent effect on DNA damage in fallopian tube epithelial cells. Heliyon. 10 (6), e27336 (2024).","journal-title":"Heliyon"},{"issue":"5","key":"ref51","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/BF01132173","type":"journal-article","article-title":"Hyaluronidase removal of the cumulus oophorus increases in vitro fertilization","volume":"5","author":"Lavy","year":"1988","unstructured":"Lavy, G., Boyers, S. P., Decherney, A. H. Hyaluronidase removal of the cumulus oophorus increases in vitro fertilization. J In Vitro Fert Embryo Transf. 5 (5), 257-260 (1988).","journal-title":"J In Vitro Fert Embryo Transf"},{"issue":"1","key":"ref52","doi-asserted-by":"crossref","first-page":"27","DOI":"10.5935\/1518-0557.20170008","type":"journal-article","article-title":"Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality","volume":"21","author":"Moura","year":"2017","unstructured":"Moura, B. R. et al. Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality. JBRA Assist Reprod. 21 (1), 27-30 (2017).","journal-title":"JBRA Assist Reprod"},{"issue":"4","key":"ref53","doi-asserted-by":"crossref","first-page":"e22843","DOI":"10.1096\/fj.202201746RR","type":"journal-article","article-title":"A single-cell gene expression atlas of human follicular aspirates: Identification of leukocyte subpopulations and their paracrine factors","volume":"37","author":"Choi","year":"2023","unstructured":"Choi, Y. et al. A single-cell gene expression atlas of human follicular aspirates: Identification of leukocyte subpopulations and their paracrine factors. FASEB J. 37 (4), e22843 (2023).","journal-title":"FASEB J"},{"issue":"8","key":"ref54","doi-asserted-by":"crossref","first-page":"e0136473","DOI":"10.1371\/journal.pone.0136473","type":"journal-article","article-title":"Transcriptomic analysis and meta-analysis of human granulosa and cumulus cells","volume":"10","author":"Burnik Papler","year":"2015","unstructured":"Burnik Papler, T., Vrtacnik Bokal, E., Maver, A., Kopitar, A. N., Lovrecic, L. Transcriptomic analysis and meta-analysis of human granulosa and cumulus cells. PLoS One. 10 (8), e0136473 (2015).","journal-title":"PLoS One"},{"issue":"4","key":"ref55","doi-asserted-by":"crossref","first-page":"100930","DOI":"10.1016\/j.mcpro.2025.100930","type":"journal-article","article-title":"Proteomic analysis of human follicular fluid-derived exosomes reveals that insufficient folliculogenesis in aging women is associated with infertility","volume":"24","author":"Liu","year":"2025","unstructured":"Liu, Z. et al. Proteomic analysis of human follicular fluid-derived exosomes reveals that insufficient folliculogenesis in aging women is associated with infertility. Mol Cell Proteomics. 24 (4), 100930 (2025).","journal-title":"Mol Cell Proteomics"},{"issue":"1","key":"ref56","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1186\/s13048-020-00696-7","type":"journal-article","article-title":"Transcriptomics of cumulus cells - a window into oocyte maturation in humans","volume":"13","author":"Wyse","year":"2020","unstructured":"Wyse, B. A. et al. Transcriptomics of cumulus cells - a window into oocyte maturation in humans. J Ovarian Res. 13 (1), 93 (2020).","journal-title":"J Ovarian Res"},{"issue":"8","key":"ref57","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1093\/molehr\/gau031","type":"journal-article","article-title":"Characterization of the human cumulus cell transcriptome during final follicular maturation and ovulation","volume":"20","author":"Yerushalmi","year":"2014","unstructured":"Yerushalmi, G. M. et al. Characterization of the human cumulus cell transcriptome during final follicular maturation and ovulation. Mol Hum Reprod. 20 (8), 719-735 (2014).","journal-title":"Mol Hum Reprod"},{"issue":"6","key":"ref58","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1007\/s10815-012-9739-5","type":"journal-article","article-title":"Development of a novel protocol for isolation and purification of human granulosa cells","volume":"29","author":"Chilvers","year":"2012","unstructured":"Chilvers, R. A., Bodenburg, Y. H., Denner, L. A., Urban, R. J. Development of a novel protocol for isolation and purification of human granulosa cells. J Assist Reprod Genet. 29 (6), 547-556 (2012).","journal-title":"J Assist Reprod Genet"},{"issue":"4","key":"ref59","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1007\/s10561-021-09929-5","type":"journal-article","article-title":"A new isolation and culture method for granulosa cells","volume":"22","author":"Zirh","year":"2021","unstructured":"Zirh, S. et al. A new isolation and culture method for granulosa cells. 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