{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:40:46Z","timestamp":1792593646186,"version":"4.5.0"},"reference-count":28,"publisher":"MyJove Corporation","issue":"226","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/69267","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:02:05Z","timestamp":1766534525000},"source":"Crossref","is-referenced-by-count":1,"title":["The Golden Apple Snail <em>Pomacea canaliculata<\/em>: From Zygotes to Stable Mutant Lines"],"prefix":"10.3791","author":[{"given":"Asmita","family":"Gattamraju","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Accorsi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,12,23]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"dev203083","DOI":"10.1242\/dev.203083","type":"journal-article","article-title":"Extraordinary model systems for regeneration","volume":"151","author":"Accorsi","year":"2024","unstructured":"Accorsi, A., Guo, L., Marshall, W. F., Mommersteeg, M. T. M., Nakajima, Y. Extraordinary model systems for regeneration. Development. 151 (1), dev203083 (2024).","journal-title":"Development"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1387\/ijdb.11902689","type":"journal-article","article-title":"The genetic control of eye development and its implications for the evolution of the various eye-types","volume":"46","author":"Gehring","year":"2002","unstructured":"Gehring, W. J. The genetic control of eye development and its implications for the evolution of the various eye-types. Int J Dev Biol. 46 (1), 65-73 (2002).","journal-title":"Int J Dev Biol"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1017\/S0952523813000035","type":"journal-article","article-title":"Eye evolution and its functional basis","volume":"30","author":"Nilsson","year":"2013","unstructured":"Nilsson, D.-E. Eye evolution and its functional basis. Vis Neurosci. 30 (1), 5\u201320 (2013).","journal-title":"Vis Neurosci"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1038\/nrn2283","type":"journal-article","article-title":"Evolution of the vertebrate eye: opsins, photoreceptors, retina and eye cup","volume":"8","author":"Lamb","year":"2007","unstructured":"Lamb, T. D., Collin, S. P., Pugh, E. N. Evolution of the vertebrate eye: opsins, photoreceptors, retina and eye cup. Nat Rev Neurosci. 8 (1), 960\u2013976 (2007).","journal-title":"Nat Rev Neurosci"},{"key":"ref5","doi-asserted-by":"crossref","DOI":"10.1093\/acprof:oso\/9780199581139.001.0001","type":"book","volume-title":"Animal eyes","author":"Land","year":"2012","unstructured":"Land, M. F., Nilsson, D.-E. Animal eyes. Oxford University Press, Oxford; New York (2012)."},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"6698","DOI":"10.1038\/s41467-025-61681-6","type":"journal-article","article-title":"A genetically tractable non-vertebrate system to study complete camera-type eye regeneration","volume":"16","author":"Accorsi","year":"2025","unstructured":"Accorsi, A. et al. A genetically tractable non-vertebrate system to study complete camera-type eye regeneration. Nat Commun. 16 (1), 6698 (2025).","journal-title":"Nat Commun"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s12915-018-0497-4","type":"journal-article","article-title":"An adaptable chromosome preparation methodology for use in invertebrate research organisms","volume":"16","author":"Guo","year":"2018","unstructured":"Guo, L. et al. An adaptable chromosome preparation methodology for use in invertebrate research organisms. BMC Biol. 16 (1), 25 (2018).","journal-title":"BMC Biol"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"giy101","DOI":"10.1093\/gigascience\/giy101","type":"journal-article","article-title":"The genome of the golden apple snail Pomacea canaliculata provides insight into stress tolerance and invasive adaptation","volume":"7","author":"Liu","year":"2018","unstructured":"Liu, C. et al. The genome of the golden apple snail Pomacea canaliculata provides insight into stress tolerance and invasive adaptation. Gigascience. 7 (1), giy101 (2018).","journal-title":"Gigascience"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1093\/molbev\/msz084","type":"journal-article","article-title":"Signatures of divergence, invasiveness, and terrestrialization revealed by four apple snail genomes","volume":"36","author":"Sun","year":"2019","unstructured":"Sun, J. et al. Signatures of divergence, invasiveness, and terrestrialization revealed by four apple snail genomes. Mol Biol Evol. 36 (1), 1507\u20131520 (2019).","journal-title":"Mol Biol Evol"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"49","DOI":"10.32604\/biocell.2009.33.049","type":"journal-article","article-title":"Development beyond the gastrula stage and digestive organogenesis in the apple-snail Pomacea canaliculata (Architaenioglossa, Ampullariidae)","volume":"33","author":"Koch","year":"2009","unstructured":"Koch, E., Winik, B. C., Castro-Vazquez, A. Development beyond the gastrula stage and digestive organogenesis in the apple-snail Pomacea canaliculata (Architaenioglossa, Ampullariidae). Biocell. 33 (1), 49\u201365 (2009).","journal-title":"Biocell"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"2774","DOI":"10.1016\/j.cub.2023.05.066","type":"journal-article","article-title":"Creation of an albino squid line by CRISPR-Cas9 and its application for in vivo functional imaging of neural activity","volume":"33","author":"Ahuja","year":"2023","unstructured":"Ahuja, N. et al. Creation of an albino squid line by CRISPR-Cas9 and its application for in vivo functional imaging of neural activity. Curr Biol. 33 (1), 2774\u20132783.e5 (2023).","journal-title":"Curr Biol"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"1258096","DOI":"10.1126\/science.1258096","type":"journal-article","article-title":"Genome editing. The new frontier of genome engineering with CRISPR-Cas9","volume":"346","author":"Doudna","year":"2014","unstructured":"Doudna, J. A., Charpentier, E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science. 346 (1), 1258096 (2014).","journal-title":"Science"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"5486","DOI":"10.1038\/ncomms6486","type":"journal-article","article-title":"TALEN and CRISPR\/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis","volume":"5","author":"Ikmi","year":"2014","unstructured":"Ikmi, A., McKinney, S. A., Delventhal, K. M., Gibson, M. C. TALEN and CRISPR\/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis. Nat Commun. 5 (1), 5486 (2014).","journal-title":"Nat Commun"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1002\/dvg.22843","type":"journal-article","article-title":"CRISPR\/Cas9-mediated genome modification in the mollusc, Crepidula fornicata","volume":"53","author":"Perry","year":"2015","unstructured":"Perry, K. J., Henry, J. Q. CRISPR\/Cas9-mediated genome modification in the mollusc, Crepidula fornicata. Genesis. 53 (1), 237\u2013244 (2015).","journal-title":"Genesis"},{"issue":"1","key":"ref15","doi-asserted-by":"crossref","first-page":"dev175976","DOI":"10.1242\/dev.175976","type":"journal-article","article-title":"The development of CRISPR for a mollusc establishes the formin Lsdia1 as the long-sought gene for snail dextral\/sinistral coiling","volume":"146","author":"Abe","year":"2019","unstructured":"Abe, M., Kuroda, R. The development of CRISPR for a mollusc establishes the formin Lsdia1 as the long-sought gene for snail dextral\/sinistral coiling. Development. 146 (1), dev175976 (2019).","journal-title":"Development"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1002\/jez.b.23293","type":"journal-article","article-title":"CRISPR\/Cas9 knockout of shell matrix protein 1 in the slipper\u2010snail Crepidula atrasolea","volume":"344","author":"Batzel","year":"2025","unstructured":"Batzel, G. et al. CRISPR\/Cas9 knockout of shell matrix protein 1 in the slipper\u2010snail Crepidula atrasolea. J Exp Zool Pt B. 344 (1), 266\u2013283 (2025).","journal-title":"J Exp Zool Pt B"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s10126-019-09885-y","type":"journal-article","article-title":"Targeted gene disruption in Pacific oyster based on CRISPR\/Cas9 ribonucleoprotein complexes","volume":"21","author":"Yu","year":"2019","unstructured":"Yu, H. et al. Targeted gene disruption in Pacific oyster based on CRISPR\/Cas9 ribonucleoprotein complexes. Mar Biotechnol. 21 (1), 301\u2013309 (2019).","journal-title":"Mar Biotechnol"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/s12052-008-0084-1","type":"journal-article","article-title":"Charting evolution\u2019s trajectory: Using molluscan eye diversity to understand parallel and convergent evolution","volume":"1","author":"Serb","year":"2008","unstructured":"Serb, J. M., Eernisse, D. J. Charting evolution\u2019s trajectory: Using molluscan eye diversity to understand parallel and convergent evolution. Evo Edu Outreach. 1 (1), 439\u2013447 (2008).","journal-title":"Evo Edu Outreach"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"3810","DOI":"10.1111\/mec.13252","type":"journal-article","article-title":"Connecting genotypes, phenotypes and fitness: Harnessing the power of CRISPR\/Cas9 genome editing","volume":"24","author":"Bono","year":"2015","unstructured":"Bono, J. M., Olesnicky, E. C., Matzkin, L. M. Connecting genotypes, phenotypes and fitness: Harnessing the power of CRISPR\/Cas9 genome editing. Mol Ecol. 24 (1), 3810\u20133822 (2015).","journal-title":"Mol Ecol"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"245","DOI":"10.4002\/040.058.0209","type":"journal-article","article-title":"Insights from an integrated view of the biology of apple snails (Caenogastropoda: Ampullariidae)","volume":"58","author":"Hayes","year":"2015","unstructured":"Hayes, K. A. et al. Insights from an integrated view of the biology of apple snails (Caenogastropoda: Ampullariidae). Malacologia. 58 (1), 245\u2013302 (2015).","journal-title":"Malacologia"},{"issue":"1","key":"ref21","first-page":"83","type":"journal-article","article-title":"Pomacea canaliculata (Gastropoda: Ampullariidae): Life-history traits and their plasticity","volume":"26","author":"Estebenet","year":"2002","unstructured":"Estebenet, A. L., Mart\u00edn, P. R. Pomacea canaliculata (Gastropoda: Ampullariidae): Life-history traits and their plasticity. Biocell. 26 (1), 83\u201389 (2002).","journal-title":"Biocell"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.cbpb.2013.11.008","type":"journal-article","article-title":"The major egg reserve protein from the invasive apple snail Pomacea maculata is a complex carotenoprotein related to those of Pomacea canaliculata and Pomacea scalaris","volume":"169","author":"Pasquevich","year":"2014","unstructured":"Pasquevich, M. Y., Dreon, M. S., Heras, H. The major egg reserve protein from the invasive apple snail Pomacea maculata is a complex carotenoprotein related to those of Pomacea canaliculata and Pomacea scalaris. Comp Biochem Physiol B Biochem Mol Biol. 169 (1), 63\u201371 (2014).","journal-title":"Comp Biochem Physiol B Biochem Mol Biol"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s00435-012-0183-y","type":"journal-article","article-title":"Functional anatomy of male copulatory organs of Pomacea canaliculata (Caenogastropoda, Ampullariidae)","volume":"132","author":"Giraud-Billoud","year":"2013","unstructured":"Giraud-Billoud, M., Gamarra-Luques, C., Castro-Vazquez, A. Functional anatomy of male copulatory organs of Pomacea canaliculata (Caenogastropoda, Ampullariidae). Zoomorphology. 132 (1), 129\u2013143 (2013).","journal-title":"Zoomorphology"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.dib.2017.09.020","type":"journal-article","article-title":"Dataset for the proteomic and transcriptomic analyses of perivitelline fluid proteins in Pomacea snail eggs","volume":"15","author":"Mu","year":"2017","unstructured":"Mu, H., Sun, J., Heras, H., Chu, K. H., Qiu, J.-W. Dataset for the proteomic and transcriptomic analyses of perivitelline fluid proteins in Pomacea snail eggs. Data Brief. 15 (1), 203\u2013207 (2017).","journal-title":"Data Brief"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"3168","DOI":"10.1002\/dvdy.22143","type":"journal-article","article-title":"A rapid and efficient method of genotyping zebrafish mutants","volume":"238","author":"Parant","year":"2009","unstructured":"Parant, J. M., George, S. A., Pryor, R., Wittwer, C. T., Yost, H. J. A rapid and efficient method of genotyping zebrafish mutants. Dev Dyn. 238 (1), 3168\u20133174 (2009).","journal-title":"Dev Dyn"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1186\/2193-1801-2-312","type":"journal-article","article-title":"Juvenile growth and survival of the apple snail Pomacea canaliculata (Caenogastropoda: Ampullariidae) reared at different constant temperatures","volume":"2","author":"Seuffert","year":"2013","unstructured":"Seuffert, M. E., Mart\u00edn, P. R. Juvenile growth and survival of the apple snail Pomacea canaliculata (Caenogastropoda: Ampullariidae) reared at different constant temperatures. Springerplus. 2 (1), 312 (2013).","journal-title":"Springerplus"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"aaf7907","DOI":"10.1126\/science.aaf7907","type":"journal-article","article-title":"Whole-organism lineage tracing by combinatorial and cumulative genome editing","volume":"353","author":"McKenna","year":"2016","unstructured":"McKenna, A. et al. Whole-organism lineage tracing by combinatorial and cumulative genome editing. Science. 353 (1), aaf7907 (2016).","journal-title":"Science"},{"issue":"1","key":"ref28","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1038\/s41580-019-0186-3","type":"journal-article","article-title":"Unravelling cellular relationships during development and regeneration using genetic lineage tracing","volume":"20","author":"Baron","year":"2019","unstructured":"Baron, C. S., Van Oudenaarden, A. Unravelling cellular relationships during development and regeneration using genetic lineage tracing. Nat Rev Mol Cell Biol. 20 (1), 753\u2013765 (2019).","journal-title":"Nat Rev Mol Cell Biol"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/69267\/the-golden-apple-snail-pomacea-canaliculata-from-zygotes-to-stable","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:59:59Z","timestamp":1792591199000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/69267\/the-golden-apple-snail-pomacea-canaliculata-from-zygotes-to-stable"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,23]]},"references-count":28,"journal-issue":{"issue":"226","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/69267","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,23]]}}}