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A fast and easy method to determine the production of reactive oxygen intermediates by human and murine phagocytes using dihydrorhodamine 123. J Immunol Methods. 131 (2), 269-227 (1990).","journal-title":"J Immunol Methods"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"113308","DOI":"10.1016\/j.jim.2022.113308","type":"journal-article","article-title":"Optimized flow cytometry protocol for dihydrorhodamine 123-based detection of reactive oxygen species in leukocyte subpopulations in whole blood","volume":"507","author":"Pioch","year":"2022","unstructured":"Pioch, J., Blomgran, R. Optimized flow cytometry protocol for dihydrorhodamine 123-based detection of reactive oxygen species in leukocyte subpopulations in whole blood. J Immunol Methods. 507, 113308 (2022).","journal-title":"J Immunol Methods"},{"issue":"7","key":"ref52","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1111\/jth.15313","type":"journal-article","article-title":"Pad4 takes charge during neutrophil activation: Impact of pad4 mediated net formation on immune-mediated disease","volume":"19","author":"Liu","year":"2021","unstructured":"Liu, X. et al. Pad4 takes charge during neutrophil activation: Impact of pad4 mediated net formation on immune-mediated disease. J Thromb Haemost. 19 (7), 1607-1617 (2021).","journal-title":"J Thromb Haemost"},{"issue":"2","key":"ref53","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1083\/jcb.200806072","type":"journal-article","article-title":"Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation","volume":"184","author":"Wang","year":"2009","unstructured":"Wang, Y. et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J Cell Biol. 184 (2), 205-213 (2009).","journal-title":"J Cell Biol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"277","DOI":"10.3389\/fimmu.2016.00277","type":"journal-article","article-title":"Transforming growth factor-\u03b2-activated kinase 1 is required for human fc\u03b3riiib-induced neutrophil extracellular trap formation","volume":"7","author":"Alem\u00e1n","year":"2016","unstructured":"Alem\u00e1n, O. R., Mora, N., Cortes-Vieyra, R., Uribe-Querol, E., Rosales, C. Transforming growth factor-\u03b2-activated kinase 1 is required for human fc\u03b3riiib-induced neutrophil extracellular trap formation. Front Immunol. 7, 277 (2016).","journal-title":"Front Immunol"},{"issue":"6","key":"ref55","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/j.autrev.2016.02.018","type":"journal-article","article-title":"A novel method for high-throughput detection and quantification of neutrophil extracellular traps reveals ROS-independent net release with immune complexes","volume":"15","author":"Kraaij","year":"2016","unstructured":"Kraaij, T. et al. A novel method for high-throughput detection and quantification of neutrophil extracellular traps reveals ROS-independent net release with immune complexes. Autoimmun Rev. 15 (6), 577-584 (2016).","journal-title":"Autoimmun Rev"},{"key":"ref56","type":"book","volume-title":"Neutrophil methods and protocols","author":"Sanchez Gonzalez","year":"2014","unstructured":"Sanchez Gonzalez, A., Bardoel, B. W., Harbort, C. J., Zychlinsky, A. Neutrophil methods and protocols. Humana Press - Springer Science+Business Media, New York (2014)."},{"issue":"1","key":"ref57","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.imbio.2016.02.001","type":"journal-article","article-title":"The diversity of circulating neutrophils in cancer","volume":"222","author":"Mishalian","year":"2017","unstructured":"Mishalian, I., Granot, Z., Fridlender, Z. G. The diversity of circulating neutrophils in cancer. Immunobiology. 222 (1), 82-88 (2017).","journal-title":"Immunobiology"},{"issue":"2","key":"ref58","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1189\/jlb.5A0116-022R","type":"journal-article","article-title":"Low-density granulocytes: Functionally distinct, immature neutrophils in rheumatoid arthritis with altered properties and defective TNF signalling","volume":"101","author":"Wright","year":"2017","unstructured":"Wright, H. L., Makki, F. A., Moots, R. J., Edwards, S. W. Low-density granulocytes: Functionally distinct, immature neutrophils in rheumatoid arthritis with altered properties and defective TNF signalling. J Leukoc Biol. 101 (2), 599-611 (2017).","journal-title":"J Leukoc Biol"},{"issue":"4","key":"ref59","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1007\/s00262-018-2170-8","type":"journal-article","article-title":"How to measure the immunosuppressive activity of mdsc: Assays, problems and potential solutions","volume":"68","author":"Bruger","year":"2019","unstructured":"Bruger, A. M. et al. How to measure the immunosuppressive activity of mdsc: Assays, problems and potential solutions. Cancer Immunol Immunother. 68 (4), 631-644 (2019).","journal-title":"Cancer Immunol Immunother"},{"issue":"4","key":"ref60","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1084\/jem.171.4.1239","type":"journal-article","article-title":"The glycosyl phosphatidylinositol-linked FC\u03b3RIII PMN mediates transmembrane signaling events distinct from FC\u03b3RII","volume":"171","author":"Kimberly","year":"1990","unstructured":"Kimberly, R. P., Ahlstrom, J. W., Click, M. E., Edberg, J. C. The glycosyl phosphatidylinositol-linked FC\u03b3RIII PMN mediates transmembrane signaling events distinct from FC\u03b3RII. J Exp Med. 171 (4), 1239-1255 (1990).","journal-title":"J Exp Med"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"21","DOI":"10.4236\/abb.2013.44A004","type":"journal-article","article-title":"Fc receptors: Cell activators of antibody functions","volume":"4","author":"Rosales","year":"2013","unstructured":"Rosales, C., Uribe-Querol, E. Fc receptors: Cell activators of antibody functions. Adv Biosci Biotech. 4, 21-33 (2013).","journal-title":"Adv Biosci Biotech"},{"issue":"1","key":"ref62","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/1044-5323(95)90006-3","type":"journal-article","article-title":"Function of human fc\u03b3riia and fc\u03b3riiib","volume":"7","author":"Unkeless","year":"1995","unstructured":"Unkeless, J. C., Shen, Z., Lin, C. W., Debeus, E. Function of human fc\u03b3riia and fc\u03b3riiib. Semin Immunol. 7 (1), 37-44 (1995).","journal-title":"Semin Immunol"},{"issue":"14","key":"ref63","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.2217\/imt-2019-0040","type":"journal-article","article-title":"Natural killer cells and cancer therapy, what we know and where we are going","volume":"11","author":"Ghaemdoust","year":"2019","unstructured":"Ghaemdoust, F., Keshavarz-Fathi, M., Rezaei, N. Natural killer cells and cancer therapy, what we know and where we are going. Immunotherapy. 11 (14), 1231-1251 (2019).","journal-title":"Immunotherapy"},{"issue":"12","key":"ref64","doi-asserted-by":"crossref","first-page":"4480","DOI":"10.4049\/jimmunol.136.12.4480","type":"journal-article","article-title":"The relationship of CD16 (leu-11) and leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes","volume":"136","author":"Lanier","year":"1986","unstructured":"Lanier, L. L., Le, A. M., Civin, C. I., Loken, M. R., Phillips, J. H. The relationship of CD16 (leu-11) and leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 136 (12), 4480-4486 (1986).","journal-title":"J Immunol"},{"issue":"6174","key":"ref65","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1038\/333667a0","type":"journal-article","article-title":"The pi-linked receptor fcriii is released on stimulation of neutrophils","volume":"333","author":"Huizinga","year":"1988","unstructured":"Huizinga, T. W. et al. The pi-linked receptor fcriii is released on stimulation of neutrophils. Nature. 333 (6174), 667-669 (1988).","journal-title":"Nature"},{"issue":"46","key":"ref66","doi-asserted-by":"crossref","first-page":"45720","DOI":"10.1074\/jbc.M303704200","type":"journal-article","article-title":"Fc\u03b3-receptors induce mac-1 (cd11b\/cd18) mobilization and accumulation in the phagocytic cup for optimal phagocytosis","volume":"278","author":"Jongstra-Bilen","year":"2003","unstructured":"Jongstra-Bilen, J., Harrison, R., Grinstein, S. Fc\u03b3-receptors induce mac-1 (cd11b\/cd18) mobilization and accumulation in the phagocytic cup for optimal phagocytosis. J Biol Chem. 278 (46), 45720-45729 (2003).","journal-title":"J Biol Chem"},{"issue":"26","key":"ref67","doi-asserted-by":"crossref","first-page":"3476","DOI":"10.1182\/blood-2016-07-727669","type":"journal-article","article-title":"Human CD62Ldim neutrophils identified as a separate subset by proteome profiling and in vivo pulse-chase labeling","volume":"129","author":"Tak","year":"2017","unstructured":"Tak, T. et al. Human CD62Ldim neutrophils identified as a separate subset by proteome profiling and in vivo pulse-chase labeling. Blood. 129 (26), 3476-3485 (2017).","journal-title":"Blood"},{"issue":"2","key":"ref68","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.yexcr.2016.03.007","type":"journal-article","article-title":"Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry","volume":"342","author":"Lakschevitz","year":"2016","unstructured":"Lakschevitz, F. S. et al. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 342 (2), 200-209 (2016).","journal-title":"Exp Cell Res"},{"issue":"9","key":"ref69","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/j.humimm.2006.05.004","type":"journal-article","article-title":"Crosslinking of CD66 b on peripheral blood neutrophils mediates the release of interleukin-8 from intracellular storage","volume":"67","author":"Schr\u00f6der","year":"2006","unstructured":"Schr\u00f6der, A. K., Uciechowski, P., Fleischer, D., Rink, L. Crosslinking of CD66 b on peripheral blood neutrophils mediates the release of interleukin-8 from intracellular storage. Hum Immunol. 67 (9), 676-682 (2006).","journal-title":"Hum Immunol"},{"issue":"1","key":"ref70","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1002\/jlb.60.1.106","type":"journal-article","article-title":"Cd66a, cd66b, cd66c, and cd66d each independently stimulate neutrophils","volume":"60","author":"Skubitz","year":"1996","unstructured":"Skubitz, K. M., Campbell, K. D., Skubitz, A. P. Cd66a, cd66b, cd66c, and cd66d each independently stimulate neutrophils. J Leukoc Biol. 60 (1), 106-117 (1996).","journal-title":"J Leukoc Biol"},{"key":"ref71","volume-title":"Basic principle of magnetic cell separation","year":"2025","unstructured":"Miltenyi Biotec. Basic principle of magnetic cell separation. https:\/\/www.miltenyibiotec.com\/UN-en\/products\/macs-cell-separation\/basic-principle-of-magnetic-cell-separation.html?query=:relevance:allCategoriesOR:10000089 (2025)."},{"issue":"1","key":"ref72","doi-asserted-by":"crossref","first-page":"3667","DOI":"10.1038\/s41598-022-07455-2","type":"journal-article","article-title":"Optimization of methods for the accurate characterization of whole blood neutrophils","volume":"12","author":"Connelly","year":"2022","unstructured":"Connelly, A. N. et al. Optimization of methods for the accurate characterization of whole blood neutrophils. Sci. Rep. 12 (1), 3667 (2022).","journal-title":"Sci. Rep"},{"key":"ref73","doi-asserted-by":"crossref","first-page":"625922","DOI":"10.3389\/fimmu.2021.625922","type":"journal-article","article-title":"High purity isolation of low density neutrophils casts doubt on their exceptionality in health and disease","volume":"12","author":"Hardisty","year":"2021","unstructured":"Hardisty, G. R. et al. High purity isolation of low density neutrophils casts doubt on their exceptionality in health and disease. Front Immunol. 12, 625922 (2021).","journal-title":"Front Immunol"},{"issue":"5","key":"ref74","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.1172\/JCI84538","type":"journal-article","article-title":"Neutrophil extracellular traps - the dark side of neutrophils","volume":"126","author":"S\u00f8rensen","year":"2016","unstructured":"S\u00f8rensen, O. E., Borregaard, N. Neutrophil extracellular traps - the dark side of neutrophils. J Clin Invest. 126 (5), 1612-1620 (2016).","journal-title":"J Clin Invest"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/69407\/rapid-magnetic-microbead-method-for-efficient-purification-low","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:14:08Z","timestamp":1792592048000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/69407\/rapid-magnetic-microbead-method-for-efficient-purification-low"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,11]]},"references-count":74,"journal-issue":{"issue":"225","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/69407","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,11]]}}}