{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:40:54Z","timestamp":1792590054712,"version":"4.5.0"},"reference-count":21,"publisher":"MyJove Corporation","issue":"230","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/69455","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T23:00:40Z","timestamp":1777503640000},"source":"Crossref","is-referenced-by-count":0,"title":["Whole Mount Immunostaining of Fetal and Neonatal Mouse Ovaries"],"prefix":"10.3791","author":[{"given":"Melissa E.","family":"Pepling","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Rasmussen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaelyn","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Erickson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/978-1-0716-0958-3_10","type":"journal-article","article-title":"Whole-mount immunofluorescence staining of early mouse embryos","volume":"2214","author":"Wong","year":"2021","unstructured":"Wong, F. C. K. Whole-mount immunofluorescence staining of early mouse embryos. Methods Mol Biol. 2214, 143-155 (2021).","journal-title":"Methods Mol Biol"},{"key":"ref2","first-page":"75","type":"journal-article","article-title":"X-chromosome activity in foetal germ cells of the mouse","volume":"63","author":"Monk","year":"1981","unstructured":"Monk, M., McLaren, A. X-chromosome activity in foetal germ cells of the mouse. J Embryol Exp Morphol. 63, 75-84 (1981).","journal-title":"J Embryol Exp Morphol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"3323","DOI":"10.1242\/dev.125.17.3323","type":"journal-article","article-title":"Female mouse germ cells form synchronously dividing cysts","volume":"125","author":"Pepling","year":"1998","unstructured":"Pepling, M. E., Spradling, A. C. Female mouse germ cells form synchronously dividing cysts. Development. 125, 3323-3328 (1998).","journal-title":"Development"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1006\/dbio.2001.0269","type":"journal-article","article-title":"Mouse ovarian germ cell cysts undergo programmed breakdown to form primordial follicles","volume":"234","author":"Pepling","year":"2001","unstructured":"Pepling, M. E., Spradling, A. C. Mouse ovarian germ cell cysts undergo programmed breakdown to form primordial follicles. Dev Biol. 234 (2), 339-351 (2001).","journal-title":"Dev Biol"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1186\/s12958-019-0548-x","type":"journal-article","article-title":"Molecular analysis of the effects of steroid hormones on mouse meiotic prophase I progression","volume":"17","author":"Burks","year":"2019","unstructured":"Burks, D. M. et al. Molecular analysis of the effects of steroid hormones on mouse meiotic prophase I progression. Reprod Biol Endocrinol. 17 (1), 105 (2019).","journal-title":"Reprod Biol Endocrinol"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1677\/JOE-09-0109","type":"journal-article","article-title":"Estrogen regulates oocyte cyst breakdown and primordial follicle assembly via multiple pathways in the neonatal mouse ovary","volume":"202","author":"Chen","year":"2009","unstructured":"Chen, Y., Breen, K., Pepling, M. E. Estrogen regulates oocyte cyst breakdown and primordial follicle assembly via multiple pathways in the neonatal mouse ovary. J Endocrinol. 202 (3), 407-417 (2009).","journal-title":"J Endocrinol"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1095\/biolreprod.114.119214","type":"journal-article","article-title":"The steroid hormone environment during primordial follicle formation in perinatal mouse ovaries","volume":"91","author":"Dutta","year":"2014","unstructured":"Dutta, S., Mark-Kappeler, C. J., Hoyer, P. B., Pepling, M. E. The steroid hormone environment during primordial follicle formation in perinatal mouse ovaries. Biol Reprod. 91 (3), 68 (2014).","journal-title":"Biol Reprod"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.ydbio.2013.06.030","type":"journal-article","article-title":"Kit signaling regulates primordial follicle formation in the neonatal mouse ovary","volume":"382","author":"Jones","year":"2013","unstructured":"Jones, R. L., Pepling, M. E. Kit signaling regulates primordial follicle formation in the neonatal mouse ovary. Dev Biol. 382 (1), 186-197 (2013).","journal-title":"Dev Biol"},{"issue":"4","key":"ref9","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.modgep.2004.12.007","type":"journal-article","article-title":"Expression of STAT3 in germ cells of developing and adult mouse ovaries and testes","volume":"5","author":"Murphy","year":"2005","unstructured":"Murphy, K., Carvajal, L., Medico, L., Pepling, M. E. Expression of STAT3 in germ cells of developing and adult mouse ovaries and testes. Gene Expr Patterns. 5 (4), 475-482 (2005).","journal-title":"Gene Expr Patterns"},{"issue":"8","key":"ref10","doi-asserted-by":"crossref","first-page":"3580","DOI":"10.1210\/en.2007-0088","type":"journal-article","article-title":"Estradiol, progesterone, and genistein inhibit oocyte nest breakdown and primordial follicle assembly","volume":"148","author":"Chen","year":"2007","unstructured":"Chen, Y., Jefferson, W. N., Newbold, R. R., Padilla-Banks, E., Pepling, M. E. Estradiol, progesterone, and genistein inhibit oocyte nest breakdown and primordial follicle assembly. Endocrinology. 148 (8), 3580-3590 (2007).","journal-title":"Endocrinology"},{"issue":"2","key":"ref11","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1530\/REP-09-0392","type":"journal-article","article-title":"Differences in oocyte development and estradiol sensitivity among mouse strains","volume":"139","author":"Pepling","year":"2010","unstructured":"Pepling, M. E. et al. Differences in oocyte development and estradiol sensitivity among mouse strains. Reproduction. 139 (2), 349-357 (2010).","journal-title":"Reproduction"},{"issue":"3","key":"ref12","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1093\/biolre\/ioab204","type":"journal-article","article-title":"Regulation of mouse primordial follicle formation by PI3K signaling","volume":"106","author":"Burton","year":"2022","unstructured":"Burton, J. J. N., Luke, A. J., Pepling, M. E. Regulation of mouse primordial follicle formation by PI3K signaling. Biol Reprod. 106 (3), 515-525 (2022).","journal-title":"Biol Reprod"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1369\/jhc.2010.956920","type":"journal-article","article-title":"Controls for immunocytochemistry: An update","volume":"59","author":"Burry","year":"2011","unstructured":"Burry, R. W. Controls for immunocytochemistry: An update. J Histochem Cytochem. 59 (1), 6-12 (2011).","journal-title":"J Histochem Cytochem"},{"issue":"3","key":"ref14","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.3390\/ijms23031426","type":"journal-article","article-title":"Ten approaches that improve immunostaining: advances in immunofluorescence optimization","volume":"23","author":"Pina","year":"2022","unstructured":"Pina, R. et al. Ten approaches that improve immunostaining: advances in immunofluorescence optimization. Int J Mol Sci. 23 (3), 1426 (2022).","journal-title":"Int J Mol Sci"},{"issue":"5","key":"ref15","doi-asserted-by":"crossref","first-page":"100458","DOI":"10.1016\/j.crmeth.2023.100458","type":"journal-article","article-title":"Principles of deep immunohistochemistry for 3D histology","volume":"3","author":"Yau","year":"2023","unstructured":"Yau, C. N. et al. Principles of deep immunohistochemistry for 3D histology. Cell Rep Methods. 3 (5), 100458 (2023).","journal-title":"Cell Rep Methods"},{"key":"ref16","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-642-04609-4","type":"book","volume-title":"Immunohistochemistry: Basics and Methods","author":"Buchwalow","year":"2010","unstructured":"Buchwalow, I. B., B\u00f6cker, W. Immunohistochemistry: Basics and Methods. Springer-Verlag (2010)."},{"issue":"9","key":"ref17","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1369\/jhc.3A6205.2004","type":"journal-article","article-title":"Antigen retrieval by heating for double-label fluorescent immunohistochemistry with identical species-derived antibodies","volume":"52","author":"Ino","year":"2004","unstructured":"Ino, H. Antigen retrieval by heating for double-label fluorescent immunohistochemistry with identical species-derived antibodies. J Histochem Cytochem. 52 (9), 1209-1217 (2004).","journal-title":"J Histochem Cytochem"},{"issue":"5","key":"ref18","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1530\/rep.1.00095","type":"journal-article","article-title":"Methods for quantifying follicular numbers within the mouse ovary","volume":"127","author":"Myers","year":"2004","unstructured":"Myers, M., Britt, K. L., Wreford, N. G., Ebling, F. J., Kerr, J. B. Methods for quantifying follicular numbers within the mouse ovary. Reproduction. 127 (5), 569-580 (2004).","journal-title":"Reproduction"},{"issue":"175","key":"ref19","first-page":"e62972","type":"journal-article","article-title":"Whole ovary immunofluorescence, clearing, and multiphoton microscopy for 3D analysis of the ovarian reserve in mouse","author":"Boateng","year":"2021","unstructured":"Boateng, R. et al. Whole ovary immunofluorescence, clearing, and multiphoton microscopy for 3D analysis of the ovarian reserve in mouse. J Vis Exp. 175, e62972 (2021).","journal-title":"J Vis Exp"},{"issue":"5","key":"ref20","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1093\/biolre\/ioaf023","type":"journal-article","article-title":"Oocount: A machine-learning-based approach to mouse ovarian follicle counting and classification","volume":"113","author":"Folts","year":"2025","unstructured":"Folts, L. et al. Oocount: A machine-learning-based approach to mouse ovarian follicle counting and classification. Biol Reprod. 113 (5), 1083-1101 (2025).","journal-title":"Biol Reprod"},{"issue":"135","key":"ref21","first-page":"e57593","type":"journal-article","article-title":"Whole-mount immunofluorescence and follicle quantification of cultured mouse ovaries","author":"Rinaldi","year":"2018","unstructured":"Rinaldi, V. D., Bloom, J. C., Schimenti, J. C. Whole-mount immunofluorescence and follicle quantification of cultured mouse ovaries. J Vis Exp. 135, e57593 (2018).","journal-title":"J Vis Exp"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/69455\/whole-mount-immunostaining-of-fetal-and-neonatal-mouse-ovaries","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:55:04Z","timestamp":1792587304000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/69455\/whole-mount-immunostaining-of-fetal-and-neonatal-mouse-ovaries"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,28]]},"references-count":21,"journal-issue":{"issue":"230","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.3791\/69455","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,28]]}}}