{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:45:27Z","timestamp":1792590327902,"version":"4.5.0"},"reference-count":28,"publisher":"MyJove Corporation","issue":"229","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/69545","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T23:01:30Z","timestamp":1773442890000},"source":"Crossref","is-referenced-by-count":0,"title":["Purification of HLA-G<sup>+<\/sup> Extravillous Trophoblasts from Human Term Placental Tissues for Phenotyping and Functional Analysis"],"prefix":"10.3791","author":[{"given":"Sayaka","family":"Tsuda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"Dasenbrock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shweta","family":"Mahajan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Tilburgs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2026,3,13]]},"reference":[{"issue":"27","key":"ref1","doi-asserted-by":"crossref","first-page":"15772","DOI":"10.1073\/pnas.2000484117","type":"journal-article","article-title":"Three types of HLA-G+ extravillous trophoblasts that have distinct immune regulatory properties","volume":"117","author":"Papuchova","year":"2020","unstructured":"Papuchova, H. et al. Three types of HLA-G+ extravillous trophoblasts that have distinct immune regulatory properties. Proc Natl Acad Sci U S A. 117 (27), 15772-15777 (2020).","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"ref2","doi-asserted-by":"crossref","first-page":"102276","DOI":"10.1016\/j.xpro.2023.102276","type":"journal-article","article-title":"Utilizing primary HLA-G+ extravillous trophoblasts and HLA-G+ EVT-like cell lines to study maternal-fetal interactions","volume":"4","author":"Hamilton","year":"2023","unstructured":"Hamilton, I., Ikumi, N. M., Kshirsagar, S., Goodman, W. A., Tilburgs, T. Utilizing primary HLA-G+ extravillous trophoblasts and HLA-G+ EVT-like cell lines to study maternal-fetal interactions. STAR Protoc. 4 (2), 102276 (2023).","journal-title":"STAR Protoc"},{"issue":"23","key":"ref3","doi-asserted-by":"crossref","first-page":"7219","DOI":"10.1073\/pnas.1507977112","type":"journal-article","article-title":"Human HLA-G+ extravillous trophoblasts: Immune-activating cells that interact with decidual leukocytes","volume":"112","author":"Tilburgs","year":"2015","unstructured":"Tilburgs, T. et al. Human HLA-G+ extravillous trophoblasts: Immune-activating cells that interact with decidual leukocytes. Proc Natl Acad Sci U S A. 112 (23), 7219-7224 (2015).","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"17","key":"ref4","doi-asserted-by":"crossref","first-page":"4713","DOI":"10.1016\/j.cell.2024.06.012","type":"journal-article","article-title":"Progestogen-driven B7-H4 contributes to onco-fetal immune tolerance","volume":"187","author":"Yu","year":"2024","unstructured":"Yu, J. et al. Progestogen-driven B7-H4 contributes to onco-fetal immune tolerance. Cell. 187 (17), 4713-4732.e19 (2024).","journal-title":"Cell"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.placenta.2010.10.010","type":"journal-article","article-title":"Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells","volume":"32","author":"Apps","year":"2011","unstructured":"Apps, R. et al. Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells. Placenta. 32 (1), 33-43 (2011).","journal-title":"Placenta"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1111\/j.1365-2567.2007.02789.x","type":"journal-article","article-title":"Conformation of human leucocyte antigen-C molecules at the surface of human trophoblast cells","volume":"124","author":"Apps","year":"2008","unstructured":"Apps, R., Gardner, L., Hiby, S. E., Sharkey, A. M., Moffett, A. Conformation of human leucocyte antigen-C molecules at the surface of human trophoblast cells. Immunology. 124 (3), 322-328 (2008).","journal-title":"Immunology"},{"issue":"8","key":"ref7","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1038\/nri1897","type":"journal-article","article-title":"Immunology of placentation in eutherian mammals","volume":"6","author":"Moffett","year":"2006","unstructured":"Moffett, A., Loke, C. Immunology of placentation in eutherian mammals. Nat Rev Immunol. 6 (8), 584-594 (2006).","journal-title":"Nat Rev Immunol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"2730","DOI":"10.3389\/fimmu.2019.02730","type":"journal-article","article-title":"The dual role of HLA-C in tolerance and immunity at the maternal-fetal interface","volume":"10","author":"Pap\u00fachov\u00e1","year":"2019","unstructured":"Pap\u00fachov\u00e1, H., Meissner, T. B., Li, Q., Strominger, J. L., Tilburgs, T. The dual role of HLA-C in tolerance and immunity at the maternal-fetal interface. Front Immunol. 10, 2730 (2019).","journal-title":"Front Immunol"},{"issue":"2","key":"ref9","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1073\/pnas.1713957115","type":"journal-article","article-title":"Mixed signature of activation and dysfunction allows human decidual CD8+ T cells to provide both tolerance and immunity","volume":"115","author":"Vander Zwan","year":"2018","unstructured":"Van der Zwan, A. et al. Mixed signature of activation and dysfunction allows human decidual CD8+ T cells to provide both tolerance and immunity. Proc Natl Acad Sci U S A. 115 (2), 385-390 (2018).","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"9","key":"ref10","doi-asserted-by":"crossref","first-page":"2537","DOI":"10.1016\/j.celrep.2019.04.109","type":"journal-article","article-title":"Three types of functional regulatory T cells control T cell responses at the human maternal-fetal interface","volume":"27","author":"Salvany-Celades","year":"2019","unstructured":"Salvany-Celades, M. et al. Three types of functional regulatory T cells control T cell responses at the human maternal-fetal interface. Cell Rep. 27 (9), 2537-2547.e5 (2019).","journal-title":"Cell Rep"},{"issue":"17","key":"ref11","doi-asserted-by":"crossref","first-page":"e171806","DOI":"10.1172\/jci.insight.171806","type":"journal-article","article-title":"Antigen-specific decidual CD8+ T cells include distinct effector memory and tissue-resident memory cells","volume":"8","author":"Mahajan","year":"2023","unstructured":"Mahajan, S. et al. Antigen-specific decidual CD8+ T cells include distinct effector memory and tissue-resident memory cells. JCI Insight. 8 (17), e171806 (2023).","journal-title":"JCI Insight"},{"issue":"52","key":"ref12","doi-asserted-by":"crossref","first-page":"15072","DOI":"10.1073\/pnas.1617927114","type":"journal-article","article-title":"Expression of KIR2DS1 by decidual natural killer cells increases their ability to control placental HCMV infection","volume":"113","author":"Crespo","year":"2016","unstructured":"Crespo, \u00c2. C., Strominger, J. L., Tilburgs, T. Expression of KIR2DS1 by decidual natural killer cells increases their ability to control placental HCMV infection. Proc Natl Acad Sci U S A. 113 (52), 15072-15077 (2016).","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref13","first-page":"12381","type":"journal-article","article-title":"Pre-eclampsia: Pathophysiology and clinical implications","volume":"366","author":"Burton","year":"2019","unstructured":"Burton, G. J., Redman, C. W., Roberts, J. M., Moffett, A. Pre-eclampsia: Pathophysiology and clinical implications. BMJ. 366, l2381 (2019).","journal-title":"BMJ"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.jri.2012.12.001","type":"journal-article","article-title":"Characterization of regulatory T cells in decidua of miscarriage cases with abnormal or normal fetal chromosomal content","volume":"97","author":"Inada","year":"2013","unstructured":"Inada, K., Shima, T., Nakashima, A., Aoki, K., Ito, M., Saito, S. Characterization of regulatory T cells in decidua of miscarriage cases with abnormal or normal fetal chromosomal content. J Reprod Immunol. 97 (1), 104-111 (2013).","journal-title":"J Reprod Immunol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1934","DOI":"10.3389\/fimmu.2018.01934","type":"journal-article","article-title":"Clonally expanded decidual effector regulatory T cells increase in late gestation of normal pregnancy but not in preeclampsia","volume":"9","author":"Tsuda","year":"2018","unstructured":"Tsuda, S. et al. Clonally expanded decidual effector regulatory T cells increase in late gestation of normal pregnancy but not in preeclampsia. Front Immunol. 9, 1934 (2018).","journal-title":"Front Immunol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1401738","DOI":"10.3389\/fimmu.2024.1401738","type":"journal-article","article-title":"CD4+ T cell heterogeneity in gestational age and preeclampsia using single-cell RNA sequencing","volume":"15","author":"Tsuda","year":"2024","unstructured":"Tsuda, S. et al. CD4+ T cell heterogeneity in gestational age and preeclampsia using single-cell RNA sequencing. Front Immunol. 15, 1401738 (2024).","journal-title":"Front Immunol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"e52004","DOI":"10.7554\/eLife.52004","type":"journal-article","article-title":"Single-cell transcriptional signatures of the human placenta in term and preterm parturition","volume":"8","author":"Pique-Regi","year":"2019","unstructured":"Pique-Regi, R. et al. Single-cell transcriptional signatures of the human placenta in term and preterm parturition. eLife. 8, e52004 (2019).","journal-title":"eLife"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1142273","DOI":"10.3389\/fimmu.2023.1142273","type":"journal-article","article-title":"Single-cell RNA-seq reveals developmental deficiencies in placentation and decidualization in late-onset preeclampsia","volume":"14","author":"Yang","year":"2023","unstructured":"Yang, J. et al. Single-cell RNA-seq reveals developmental deficiencies in placentation and decidualization in late-onset preeclampsia. Front Immunol. 14, 1142273 (2023).","journal-title":"Front Immunol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.3389\/fimmu.2020.01082","type":"journal-article","article-title":"Analysis of TCR repertoire and PD-1 expression in decidual and peripheral CD8+ T cells reveals distinct immune mechanisms in miscarriage and preeclampsia","volume":"11","author":"Morita","year":"2020","unstructured":"Morita, K. et al. Analysis of TCR repertoire and PD-1 expression in decidual and peripheral CD8+ T cells reveals distinct immune mechanisms in miscarriage and preeclampsia. Front Immunol. 11, 1082 (2020).","journal-title":"Front Immunol"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/j.1365-2249.2007.03397.x","type":"journal-article","article-title":"Proportion of peripheral blood and decidual CD4+ CD25bright regulatory T cells in pre-eclampsia","volume":"149","author":"Sasaki","year":"2007","unstructured":"Sasaki, Y. et al. Proportion of peripheral blood and decidual CD4+ CD25bright regulatory T cells in pre-eclampsia. Clin Exp Immunol. 149 (1), 139-145 (2007).","journal-title":"Clin Exp Immunol"},{"issue":"5","key":"ref21","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1093\/molehr\/gah044","type":"journal-article","article-title":"Decidual and peripheral blood CD4+CD25+ regulatory T cells in early pregnancy and spontaneous abortion","volume":"10","author":"Sasaki","year":"2004","unstructured":"Sasaki, Y. Decidual and peripheral blood CD4+CD25+ regulatory T cells in early pregnancy and spontaneous abortion. Mol Hum Reprod. 10 (5), 347-353 (2004).","journal-title":"Mol Hum Reprod"},{"key":"ref22","first-page":"00043","type":"journal-article","article-title":"Single-cell assessment of primary and stem cell-derived human trophoblast organoids as placenta-modeling platforms","volume":"S1534-5807","author":"Shannon","year":"2024","unstructured":"Shannon, M. J. et al. Single-cell assessment of primary and stem cell-derived human trophoblast organoids as placenta-modeling platforms. Dev Cell. S1534-5807, 00043-1 (2024).","journal-title":"Dev Cell"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1111\/j.1365-2567.2008.03019.x","type":"journal-article","article-title":"HLA expression of primary trophoblast cells and placental cell lines","volume":"127","author":"Apps","year":"2009","unstructured":"Apps, R., Murphy, S. P., Fernando, R., Gardner, L., Ahad, T., Moffett, A. HLA expression of primary trophoblast cells and placental cell lines. Immunology. 127 (1), 26-39 (2009).","journal-title":"Immunology"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.stem.2017.11.004","type":"journal-article","article-title":"Derivation of human trophoblast stem cells","volume":"22","author":"Okae","year":"2018","unstructured":"Okae, H. et al. Derivation of human trophoblast stem cells. Cell Stem Cell. 22 (1), 50-63.e6 (2018).","journal-title":"Cell Stem Cell"},{"issue":"51","key":"ref25","doi-asserted-by":"crossref","first-page":"e2311372120","DOI":"10.1073\/pnas.2311372120","type":"journal-article","article-title":"CRISPR screening in human trophoblast stem cells reveals shared and distinct aspects of placental development","volume":"120","author":"Shimizu","year":"2023","unstructured":"Shimizu, T. et al. CRISPR screening in human trophoblast stem cells reveals shared and distinct aspects of placental development. Proc Natl Acad Sci U S A. 120 (51), e2311372120 (2023).","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1038\/s41467-024-45279-y","type":"journal-article","article-title":"Trophoblast stem cell-based organoid models of the human placental barrier","volume":"15","author":"Hori","year":"2024","unstructured":"Hori, T. et al. Trophoblast stem cell-based organoid models of the human placental barrier. Nat Commun. 15 (1), 962 (2024).","journal-title":"Nat Commun"},{"issue":"2","key":"ref27","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1038\/s41588-023-01647-w","type":"journal-article","article-title":"Single-nucleus multi-omic profiling of human placental syncytiotrophoblasts","volume":"56","author":"Wang","year":"2024","unstructured":"Wang, M. et al. Single-nucleus multi-omic profiling of human placental syncytiotrophoblasts. Nat Genet. 56 (2), 294-305 (2024).","journal-title":"Nat Genet"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"102277","DOI":"10.1016\/j.xpro.2023.102277","type":"journal-article","article-title":"Purification of primary human placental leukocytes","volume":"4","author":"Ikumi","year":"2023","unstructured":"Ikumi, N. M., Koenig, Z., Mahajan, S., Gray, C. M., Tilburgs, T. Purification of primary human placental leukocytes. STAR Protoc. 4 (2), 102277 (2023).","journal-title":"STAR Protoc"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/69545\/purification-hla-g-extravillous-trophoblasts-from-human-term","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:16:16Z","timestamp":1792588576000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/69545\/purification-hla-g-extravillous-trophoblasts-from-human-term"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,13]]},"references-count":28,"journal-issue":{"issue":"229","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.3791\/69545","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,13]]}}}