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Osteoarthritis Cartilage. 31 (8), 1111-1120 (2023).","journal-title":"Osteoarthritis Cartilage"},{"issue":"7","key":"ref55","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1177\/03635465231169526","type":"journal-article","article-title":"Synovial fluid proteomics from serial aspirations of ACL-injured knees identifies candidate biomarkers","volume":"51","author":"Rai","year":"2023","unstructured":"Rai, M. F., Cai, L., Zhang, Q., Townsend, R.R., Brophy, R.H. Synovial fluid proteomics from serial aspirations of ACL-injured knees identifies candidate biomarkers. 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Sci Rep. 15 (1), 30105 (2025).","journal-title":"Sci Rep"},{"issue":"2","key":"ref61","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.jisako.2021.10.002","type":"journal-article","article-title":"Tibial tubercle osteotomy for patellofemoral malalignment and chondral disease provided good outcomes: A systematic review","volume":"7","author":"Rosso","year":"2022","unstructured":"Rosso, F., Rossi, R., Cottino, U., Bonasia, D. E. Tibial tubercle osteotomy for patellofemoral malalignment and chondral disease provided good outcomes: A systematic review. J ISAKOS. 7 (2), 78-86 (2022).","journal-title":"J ISAKOS"},{"issue":"4","key":"ref62","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1002\/jor.24789","type":"journal-article","article-title":"An ex vivo human osteochondral culture model","volume":"39","author":"Kleuskens","year":"2021","unstructured":"Kleuskens, M. W. A., Van Donkelaar, C.C., Kock, L.M., Janssen, R.P.A., Ito, K. 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