{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:39:02Z","timestamp":1792589942077,"version":"4.5.0"},"reference-count":21,"publisher":"MyJove Corporation","issue":"231","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/69825","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T23:02:44Z","timestamp":1779490964000},"source":"Crossref","is-referenced-by-count":0,"title":["Single-cell RNA Sequencing for Profiling Ganglionic and Aganglionic Colonic Segments from Patients with Hirschsprung Disease"],"prefix":"10.3791","author":[{"given":"Yan","family":"Qu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nian","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"NianJie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefeng","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2026,5,22]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1038\/s41572-023-00465-y","type":"journal-article","article-title":"Hirschsprung disease","volume":"9","author":"Montalva","year":"2023","unstructured":"Montalva L et al. Hirschsprung disease. Nat Rev Dis Primers. 2023;9(1):54.","journal-title":"Nat Rev Dis Primers"},{"key":"ref2","first-page":"S66","type":"journal-article","article-title":"Hirschsprung's disease management","volume":"60 Suppl 1","author":"Morera","year":"2024","unstructured":"Morera C, Nurko S. Hirschsprung's disease management. Aliment Pharmacol Ther. 2024;60 Suppl 1:S66-S76.","journal-title":"Aliment Pharmacol Ther"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1186\/s13023-020-01362-3","type":"journal-article","article-title":"ERNICA guidelines for the management of rectosigmoid Hirschsprung's disease","volume":"15","author":"Kyrklund","year":"2020","unstructured":"Kyrklund K et al. ERNICA guidelines for the management of rectosigmoid Hirschsprung's disease. Orphanet J Rare Dis. 2020;15(1):164.","journal-title":"Orphanet J Rare Dis"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"e694","DOI":"10.1002\/ctm2.694","type":"journal-article","article-title":"Single-cell RNA sequencing technologies and applications: a brief overview","volume":"12","author":"Jovic","year":"2022","unstructured":"Jovic D et al. Single-cell RNA sequencing technologies and applications: a brief overview. Clin Transl Med. 2022;12(3):e694.","journal-title":"Clin Transl Med"},{"issue":"15","key":"ref5","doi-asserted-by":"crossref","first-page":"1970","DOI":"10.3390\/cells12151970","type":"journal-article","article-title":"A review of single-cell RNA-seq annotation, integration, and cell-cell communication","volume":"12","author":"Cheng","year":"2023","unstructured":"Cheng C, Chen W, Jin H, Chen X. A review of single-cell RNA-seq annotation, integration, and cell-cell communication. Cells. 2023;12(15):1970.","journal-title":"Cells"},{"issue":"8026","key":"ref6","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1038\/s41586-024-07606-7","type":"journal-article","article-title":"Single-cell multiregion dissection of Alzheimer's disease","volume":"632","author":"Mathys","year":"2024","unstructured":"Mathys H et al. Single-cell multiregion dissection of Alzheimer's disease. Nature. 2024;632(8026):858-868.","journal-title":"Nature"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"4977","DOI":"10.1038\/s41467-021-25246-7","type":"journal-article","article-title":"Single-cell sequencing of immune cells from anticitrullinated peptide antibody positive and negative rheumatoid arthritis","volume":"12","author":"Wu","year":"2021","unstructured":"Wu X et al. Single-cell sequencing of immune cells from anticitrullinated peptide antibody positive and negative rheumatoid arthritis. Nat Commun. 2021;12(1):4977.","journal-title":"Nat Commun"},{"issue":"5","key":"ref8","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1016\/j.cell.2020.07.017","type":"journal-article","article-title":"Therapy-induced evolution of human lung cancer revealed by single-cell RNA sequencing","volume":"182","author":"Maynard","year":"2020","unstructured":"Maynard A et al. Therapy-induced evolution of human lung cancer revealed by single-cell RNA sequencing. Cell. 2020;182(5):1232-1251.e12.","journal-title":"Cell"},{"issue":"12","key":"ref9","doi-asserted-by":"crossref","first-page":"e1503","DOI":"10.1002\/ctm2.1503","type":"journal-article","article-title":"Immunological link between periodontitis and type 2 diabetes deciphered by single-cell RNA analysis","volume":"13","author":"Lee","year":"2023","unstructured":"Lee H et al. Immunological link between periodontitis and type 2 diabetes deciphered by single-cell RNA analysis. Clin Transl Med. 2023;13(12):e1503.","journal-title":"Clin Transl Med"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","type":"journal-article","article-title":"KEGG: Kyoto encyclopedia of genes and genomes","volume":"28","author":"Kanehisa","year":"2000","unstructured":"Kanehisa M, Goto S. KEGG: Kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 2000;28(1):27-30.","journal-title":"Nucleic Acids Res"},{"issue":"10","key":"ref11","doi-asserted-by":"crossref","first-page":"4163","DOI":"10.4049\/jimmunol.1502193","type":"journal-article","article-title":"Efficient culture of human naive and memory B cells for use as APCs","volume":"197","author":"Su","year":"2016","unstructured":"Su KY, Watanabe A, Yeh CH, Kelsoe G, Kuraoka M. Efficient culture of human naive and memory B cells for use as APCs. J Immunol. 2016;197(10):4163-4176.","journal-title":"J Immunol"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1111\/imr.13016","type":"journal-article","article-title":"Memory B cells and plasma cells: the differentiative continuum of humoral immunity","volume":"303","author":"Cancro","year":"2021","unstructured":"Cancro MP, Tomayko MM. Memory B cells and plasma cells: the differentiative continuum of humoral immunity. Immunol Rev. 2021;303(1):72-82.","journal-title":"Immunol Rev"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/s41590-021-01078-x","type":"journal-article","article-title":"Surface phenotypes of naive and memory B cells in mouse and human tissues","volume":"23","author":"Weisel","year":"2022","unstructured":"Weisel NM et al. Surface phenotypes of naive and memory B cells in mouse and human tissues. Nat Immunol. 2022;23(1):135-145.","journal-title":"Nat Immunol"},{"issue":"3","key":"ref14","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1038\/s41596-023-00930-8","type":"journal-article","article-title":"Production and use of antigen tetramers to study antigen-specific B cells","volume":"19","author":"Phelps","year":"2024","unstructured":"Phelps A et al. Production and use of antigen tetramers to study antigen-specific B cells. Nat Protoc. 2024;19(3):727-751.","journal-title":"Nat Protoc"},{"issue":"3","key":"ref15","doi-asserted-by":"crossref","first-page":"L443","DOI":"10.1152\/ajplung.00120.2018","type":"journal-article","article-title":"Activated naive B cells promote development of malignant pleural effusion by differential regulation of Th1 and Th17 response","volume":"315","author":"Wu","year":"2018","unstructured":"Wu XZ et al. Activated naive B cells promote development of malignant pleural effusion by differential regulation of Th1 and Th17 response. Am J Physiol Lung Cell Mol Physiol. 2018;315(3):L443-L455.","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s12929-017-0391-3","type":"journal-article","article-title":"Regulatory T cells induced by B cells: a novel subpopulation of regulatory T cells","volume":"24","author":"Chien","year":"2017","unstructured":"Chien CH, Chiang BL. Regulatory T cells induced by B cells: a novel subpopulation of regulatory T cells. J Biomed Sci. 2017;24(1):86.","journal-title":"J Biomed Sci"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1186\/s12967-023-04056-z","type":"journal-article","article-title":"Comprehensive analysis of scRNA-seq and bulk RNA-seq reveals dynamic changes in the tumor immune microenvironment of bladder cancer and establishes a prognostic model","volume":"21","author":"Tan","year":"2023","unstructured":"Tan Z et al. Comprehensive analysis of scRNA-seq and bulk RNA-seq reveals dynamic changes in the tumor immune microenvironment of bladder cancer and establishes a prognostic model. J Transl Med. 2023;21(1):223.","journal-title":"J Transl Med"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1399856","DOI":"10.3389\/fimmu.2024.1399856","type":"journal-article","article-title":"Integrated analysis of single-cell RNA-seq, bulk RNA-seq, Mendelian randomization, and eQTL reveals T cell-related nomogram model and subtype classification in rheumatoid arthritis","volume":"15","author":"Ding","year":"2024","unstructured":"Ding Q et al. Integrated analysis of single-cell RNA-seq, bulk RNA-seq, Mendelian randomization, and eQTL reveals T cell-related nomogram model and subtype classification in rheumatoid arthritis. Front Immunol. 2024;15:1399856.","journal-title":"Front Immunol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"107066","DOI":"10.1016\/j.compbiomed.2023.107066","type":"journal-article","article-title":"Integrated analysis of single-cell RNA-seq and bulk RNA-seq unravels T cell-related prognostic risk model and tumor immune microenvironment modulation in triple-negative breast cancer","volume":"161","author":"Guo","year":"2023","unstructured":"Guo S et al. Integrated analysis of single-cell RNA-seq and bulk RNA-seq unravels T cell-related prognostic risk model and tumor immune microenvironment modulation in triple-negative breast cancer. Comput Biol Med. 2023;161:107066.","journal-title":"Comput Biol Med"},{"issue":"10","key":"ref20","doi-asserted-by":"crossref","first-page":"2775","DOI":"10.1111\/cns.14280","type":"journal-article","article-title":"Comprehensive analyses of brain cell communications based on multiple scRNA-seq and snRNA-seq datasets for revealing novel mechanisms in neurodegenerative diseases","volume":"29","author":"Zhang","year":"2023","unstructured":"Zhang C et al. Comprehensive analyses of brain cell communications based on multiple scRNA-seq and snRNA-seq datasets for revealing novel mechanisms in neurodegenerative diseases. CNS Neurosci Ther. 2023;29(10):2775-2786.","journal-title":"CNS Neurosci Ther"},{"issue":"1","key":"ref21","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s13046-021-01874-1","type":"journal-article","article-title":"Single-cell RNA sequencing in cancer research","volume":"40","author":"Zhang","year":"2021","unstructured":"Zhang Y et al. Single-cell RNA sequencing in cancer research. J Exp Clin Cancer Res. 2021;40(1):81.","journal-title":"J Exp Clin Cancer Res"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/69825\/single-cell-rna-sequencing-for-profiling-ganglionic-aganglionic","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:45:12Z","timestamp":1792586712000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/69825\/single-cell-rna-sequencing-for-profiling-ganglionic-aganglionic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,22]]},"references-count":21,"journal-issue":{"issue":"231","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.3791\/69825","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,22]]}}}