{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:46:59Z","timestamp":1792590419018,"version":"4.5.0"},"reference-count":13,"publisher":"MyJove Corporation","issue":"228","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/69862","type":"journal-article","created":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T00:01:55Z","timestamp":1772236915000},"source":"Crossref","is-referenced-by-count":0,"title":["An Atherosclerosis Model in Mice Induced by Both Partial Carotid Ligation and Overexpression of <em>PCSK9<\/em>"],"prefix":"10.3791","author":[{"given":"Keqiang","family":"Shu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junye","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyao","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmei","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2026,2,27]]},"reference":[{"issue":"25","key":"ref1","doi-asserted-by":"crossref","first-page":"2361","DOI":"10.1016\/j.jacc.2022.11.005","type":"journal-article","article-title":"The global burden of cardiovascular diseases and risk: A compass for future health","volume":"80","author":"Vaduganathan","year":"2022","unstructured":"Vaduganathan, M., Mensah, G. A., Turco, J. V., Fuster, V., Roth, G. A. The global burden of cardiovascular diseases and risk: A compass for future health. J Am Coll Cardiol. 80 (25), 2361-2371 (2022).","journal-title":"J Am Coll Cardiol"},{"issue":"10","key":"ref2","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1093\/eurheartj\/ehae933","type":"journal-article","article-title":"Carotid artery atherosclerosis: Mechanisms of instability and clinical implications","volume":"46","author":"Saba","year":"2025","unstructured":"Saba, L. et al. Carotid artery atherosclerosis: Mechanisms of instability and clinical implications. Eur Heart J. 46 (10), 904-921 (2025).","journal-title":"Eur Heart J"},{"issue":"33","key":"ref3","doi-asserted-by":"crossref","first-page":"3261","DOI":"10.1093\/eurheartj\/ehaf054","type":"journal-article","article-title":"Distinct inflammatory pathways shape atherosclerosis in different vascular beds","volume":"46","author":"Soehnlein","year":"2025","unstructured":"Soehnlein, O., Lutgens, E., D\u00f6ring, Y. Distinct inflammatory pathways shape atherosclerosis in different vascular beds. Eur Heart J. 46 (33), 3261-3272 (2025).","journal-title":"Eur Heart J"},{"issue":"1s","key":"ref4","doi-asserted-by":"crossref","first-page":"4s","DOI":"10.1016\/j.jvs.2021.04.073","type":"journal-article","article-title":"Society for vascular surgery clinical practice guidelines for management of extracranial cerebrovascular disease","volume":"75","author":"Aburahma","year":"2022","unstructured":"Aburahma, A. F. et al. Society for vascular surgery clinical practice guidelines for management of extracranial cerebrovascular disease. J Vasc Surg. 75 (1s), 4s-22s (2022).","journal-title":"J Vasc Surg"},{"issue":"40","key":"ref5","first-page":"e1861","type":"journal-article","article-title":"A model of disturbed flow-induced atherosclerosis in mouse carotid artery by partial ligation and a simple method of RNA isolation from carotid endothelium","author":"Nam","year":"2010","unstructured":"Nam, D. et al. A model of disturbed flow-induced atherosclerosis in mouse carotid artery by partial ligation and a simple method of RNA isolation from carotid endothelium. J Vis Exp. (40), e1861. (2010).","journal-title":"J Vis Exp"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"H1535","DOI":"10.1152\/ajpheart.00510.2009","type":"journal-article","article-title":"Partial carotid ligation is a model of acutely induced disturbed flow, leading to rapid endothelial dysfunction and atherosclerosis","volume":"297","author":"Nam","year":"2009","unstructured":"Nam, D. et al. Partial carotid ligation is a model of acutely induced disturbed flow, leading to rapid endothelial dysfunction and atherosclerosis. Am J Physiol Heart Circ Physiol. 297 (4), H1535-1543 (2009).","journal-title":"Am J Physiol Heart Circ Physiol"},{"issue":"15","key":"ref7","doi-asserted-by":"crossref","first-page":"e66","DOI":"10.1182\/blood-2010-04-278192","type":"journal-article","article-title":"Discovery of novel mechanosensitive genes in vivo using mouse carotid artery endothelium exposed to disturbed flow","volume":"116","author":"Ni","year":"2010","unstructured":"Ni, C. W. et al. Discovery of novel mechanosensitive genes in vivo using mouse carotid artery endothelium exposed to disturbed flow. Blood. 116 (15), e66-73 (2010).","journal-title":"Blood"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1161\/01.ATV.14.1.133","type":"journal-article","article-title":"Apoe-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree","volume":"14","author":"Nakashima","year":"1994","unstructured":"Nakashima, Y., Plump, A. S., Raines, E. W., Breslow, J. L., Ross, R. Apoe-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree. Arterioscler Thromb. 14 (1), 133-140 (1994).","journal-title":"Arterioscler Thromb"},{"issue":"4","key":"ref9","doi-asserted-by":"crossref","first-page":"e35835","DOI":"10.1371\/journal.pone.0035835","type":"journal-article","article-title":"Hyperlipidemia and atherosclerotic lesion development in ldlr-deficient mice on a long-term high-fat diet","volume":"7","author":"Ma","year":"2012","unstructured":"Ma, Y. et al. Hyperlipidemia and atherosclerotic lesion development in ldlr-deficient mice on a long-term high-fat diet. PLoS One. 7 (4), e35835 (2012).","journal-title":"PLoS One"},{"issue":"6","key":"ref10","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.1073\/pnas.0409736102","type":"journal-article","article-title":"Overexpression of pcsk9 accelerates the degradation of the ldlr in a post-endoplasmic reticulum compartment","volume":"102","author":"Maxwell","year":"2005","unstructured":"Maxwell, K. N., Fisher, E. A., Breslow, J. L. Overexpression of pcsk9 accelerates the degradation of the ldlr in a post-endoplasmic reticulum compartment. Proc Natl Acad Sci U S A. 102 (6), 2069-2074 (2005).","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"ref11","doi-asserted-by":"crossref","first-page":"oeac028","DOI":"10.1093\/ehjopen\/oeac028","type":"journal-article","article-title":"The aav-pcsk9 murine model of atherosclerosis and metabolic dysfunction","volume":"2","author":"Keeter","year":"2022","unstructured":"Keeter, W. C., Carter, N. M., Nadler, J. L., Galkina, E. V. The aav-pcsk9 murine model of atherosclerosis and metabolic dysfunction. Eur Heart J Open. 2 (3), oeac028 (2022).","journal-title":"Eur Heart J Open"},{"issue":"8","key":"ref12","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1038\/labinvest.2017.47","type":"journal-article","article-title":"Accelerated atherosclerosis development in c57bl6 mice by overexpressing aav-mediated pcsk9 and partial carotid ligation","volume":"97","author":"Kumar","year":"2017","unstructured":"Kumar, S., Kang, D. W., Rezvan, A., Jo, H. Accelerated atherosclerosis development in c57bl6 mice by overexpressing aav-mediated pcsk9 and partial carotid ligation. Lab Invest. 97 (8), 935-945 (2017).","journal-title":"Lab Invest"},{"issue":"3","key":"ref13","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1007\/s11357-023-00776-8","type":"journal-article","article-title":"Advancing age increases the size and severity of spontaneous atheromas in mouse models of atherosclerosis","volume":"45","author":"Gogulamudi","year":"2023","unstructured":"Gogulamudi, V. R., Durrant, J. R., Adeyemo, A. O., Ho, H. M., Walker, A. E., Lesniewski, L. A. Advancing age increases the size and severity of spontaneous atheromas in mouse models of atherosclerosis. Geroscience. 45 (3), 1913-1931 (2023).","journal-title":"Geroscience"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/69862\/an-atherosclerosis-model-mice-induced-both-partial-carotid-ligation","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:21:03Z","timestamp":1792588863000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/69862\/an-atherosclerosis-model-mice-induced-both-partial-carotid-ligation"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,27]]},"references-count":13,"journal-issue":{"issue":"228","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.3791\/69862","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,27]]}}}