{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:46:07Z","timestamp":1792590367892,"version":"4.5.0"},"reference-count":36,"publisher":"MyJove Corporation","issue":"229","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/69865","type":"journal-article","created":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T00:02:15Z","timestamp":1772841735000},"source":"Crossref","is-referenced-by-count":0,"title":["Development of an Efficient Murine Tissue Expansion Model for Skin Regeneration Research"],"prefix":"10.3791","author":[{"given":"Xing","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiyi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengling","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoqi","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujui","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xusong","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengzhou","family":"Shan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2026,3,6]]},"reference":[{"issue":"8","key":"ref1","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/s41580-024-00715-1","type":"journal-article","article-title":"Cellular and molecular mechanisms of skin wound healing","volume":"25","author":"Pe\u00f1a","year":"2024","unstructured":"Pe\u00f1a, O. A. Martin, P. Cellular and molecular mechanisms of skin wound healing. Nat Rev Mol Cell Biol. 25 (8), 599-616 (2024).","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1021\/acs.biomac.4c01715","type":"journal-article","article-title":"Material design, fabrication strategies, and the development of multifunctional hydrogel composites dressings for skin wound management","volume":"26","author":"Wu","year":"2025","unstructured":"Wu, Z. et al. Material design, fabrication strategies, and the development of multifunctional hydrogel composites dressings for skin wound management. Biomacromolecules. 26 (3), 1419-1460 (2025).","journal-title":"Biomacromolecules"},{"key":"ref3","doi-asserted-by":"crossref","DOI":"10.1002\/smll.202506259","type":"journal-article","article-title":"Pioneering 3d and 4d bioprinting strategies for advanced wound management: From design to healing","author":"Yadav","year":"2025","unstructured":"Yadav, A. K. et al. Pioneering 3d and 4d bioprinting strategies for advanced wound management: From design to healing. Small. 10.1002\/smll.202506259 e06259 (2025).","journal-title":"Small"},{"issue":"Pt 3","key":"ref4","doi-asserted-by":"crossref","first-page":"137633","DOI":"10.1016\/j.ijbiomac.2024.137633","type":"journal-article","article-title":"Extracellular matrix-based biomaterials in burn wound repair: A promising therapeutic strategy","volume":"283","author":"Song","year":"2024","unstructured":"Song, Y. T. et al. Extracellular matrix-based biomaterials in burn wound repair: A promising therapeutic strategy. Int J Biol Macromol. 283 (Pt 3), 137633 (2024).","journal-title":"Int J Biol Macromol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"123134","DOI":"10.1016\/j.biomaterials.2025.123134","type":"journal-article","article-title":"Recent advances in smart hydrogels derived from polysaccharides and their applications for wound dressing and healing","volume":"318","author":"Tian","year":"2025","unstructured":"Tian, X. et al. Recent advances in smart hydrogels derived from polysaccharides and their applications for wound dressing and healing. Biomaterials. 318, 123134 (2025).","journal-title":"Biomaterials"},{"issue":"2","key":"ref6","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1097\/00006534-195702000-00004","type":"journal-article","article-title":"The expansion of an area of skin by progressive distention of a subcutaneous balloon; use of the method for securing skin for subtotal reconstruction of the ear","volume":"19","author":"Neumann","year":"1957","unstructured":"Neumann, C. G. The expansion of an area of skin by progressive distention of a subcutaneous balloon; use of the method for securing skin for subtotal reconstruction of the ear. Plast Reconstr Surg (1946). 19 (2), 124-130 (1957).","journal-title":"Plast Reconstr Surg (1946)"},{"issue":"17","key":"ref7","doi-asserted-by":"crossref","first-page":"9622","DOI":"10.3390\/ijms23179622","type":"journal-article","article-title":"Mechanical stretch induced skin regeneration: Molecular and cellular mechanism in skin soft tissue expansion","volume":"23","author":"Guo","year":"2022","unstructured":"Guo, Y. et al. Mechanical stretch induced skin regeneration: Molecular and cellular mechanism in skin soft tissue expansion. Int J Mol Sci. 23 (17), 9622 (2022).","journal-title":"Int J Mol Sci"},{"issue":"5","key":"ref8","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1089\/fpsam.2021.0279","type":"journal-article","article-title":"Reconstruction of facial multiunit defects using expanded scalp flap with laser depilation in a group of predominantly pediatric patients","volume":"24","author":"Ding","year":"2022","unstructured":"Ding, Y. et al. Reconstruction of facial multiunit defects using expanded scalp flap with laser depilation in a group of predominantly pediatric patients. Facial Plast Surg Aesthet Med. 24 (5), 352-356 (2022).","journal-title":"Facial Plast Surg Aesthet Med"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"141e","DOI":"10.1097\/PRS.0000000000011434","type":"journal-article","article-title":"Acellular dermal matrix-assisted tissue expansion for giant congenital melanocytic nevi of the extremities and trunk in pediatric patients","volume":"155","author":"Huang","year":"2025","unstructured":"Huang, X. et al. Acellular dermal matrix-assisted tissue expansion for giant congenital melanocytic nevi of the extremities and trunk in pediatric patients. Plast Reconstr Surg. 155 (1), 141e-151e (2025).","journal-title":"Plast Reconstr Surg"},{"issue":"3","key":"ref10","doi-asserted-by":"crossref","first-page":"469e","DOI":"10.1097\/PRS.0000000000011744","type":"journal-article","article-title":"Long-term outcomes of 1989 immediate implant-based breast reconstructions: An analysis of risk factors for failure and revision surgery","volume":"155","author":"Kooijman","year":"2025","unstructured":"Kooijman, M. M. L. et al. Long-term outcomes of 1989 immediate implant-based breast reconstructions: An analysis of risk factors for failure and revision surgery. Plast Reconstr Surg. 155 (3), 469e-478e (2025).","journal-title":"Plast Reconstr Surg"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.jss.2020.03.005","type":"journal-article","article-title":"Establishment of a novel mouse model for soft tissue expansion","volume":"253","author":"Liu","year":"2020","unstructured":"Liu, S. et al. Establishment of a novel mouse model for soft tissue expansion. J Surg Res. 253, 238-244 (2020).","journal-title":"J Surg Res"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"6876","DOI":"10.1038\/s41467-025-62270-3","type":"journal-article","article-title":"The mechanotransducer piezo1 coordinates metabolism and inflammation to promote skin growth","volume":"16","author":"Xue","year":"2025","unstructured":"Xue, Y. et al. The mechanotransducer piezo1 coordinates metabolism and inflammation to promote skin growth. Nat Commun. 16 (1), 6876 (2025).","journal-title":"Nat Commun"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"214291","DOI":"10.1016\/j.bioadv.2025.214291","type":"journal-article","article-title":"Innovative applications of acellular adipose matrix derived film in skin soft tissue expansion","volume":"173","author":"Liang","year":"2025","unstructured":"Liang, B. et al. Innovative applications of acellular adipose matrix derived film in skin soft tissue expansion. Biomater Adv. 173, 214291 (2025).","journal-title":"Biomater Adv"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s12967-019-1780-z","type":"journal-article","article-title":"Macrophages are necessary for skin regeneration during tissue expansion","volume":"17","author":"Ding","year":"2019","unstructured":"Ding, J. et al. Macrophages are necessary for skin regeneration during tissue expansion. J Transl Med. 17 (1), 36 (2019).","journal-title":"J Transl Med"},{"issue":"4","key":"ref15","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1097\/PRS.0000000000007153","type":"journal-article","article-title":"Modeling tissue expansion with isogeometric analysis: Skin growth and tissue level changes in the porcine model","volume":"146","author":"Janes","year":"2020","unstructured":"Janes, L. E. et al. Modeling tissue expansion with isogeometric analysis: Skin growth and tissue level changes in the porcine model. Plast Reconstr Surg. 146 (4), 792-798 (2020).","journal-title":"Plast Reconstr Surg"},{"issue":"41","key":"ref16","doi-asserted-by":"crossref","first-page":"e11474","DOI":"10.1002\/advs.202511474","type":"journal-article","article-title":"Mechanical stretch-induced interlayer coordination between mmp2 and col17a1 exacerbates regenerative exhaustion in skin","volume":"12","author":"Sun","year":"2025","unstructured":"Sun, Y. et al. Mechanical stretch-induced interlayer coordination between mmp2 and col17a1 exacerbates regenerative exhaustion in skin. Adv Sci (Weinh). 12 (41), e11474 (2025).","journal-title":"Adv Sci (Weinh)"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1186\/s13287-021-02318-5","type":"journal-article","article-title":"A randomized, controlled clinical trial of autologous stromal vascular fraction cells transplantation to promote mechanical stretch-induced skin regeneration","volume":"12","author":"Tan","year":"2021","unstructured":"Tan, P. C. et al. A randomized, controlled clinical trial of autologous stromal vascular fraction cells transplantation to promote mechanical stretch-induced skin regeneration. Stem Cell Res Ther. 12 (1), 243 (2021).","journal-title":"Stem Cell Res Ther"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"e16767","DOI":"10.1111\/jocd.16767","type":"journal-article","article-title":"Ace2 inhibits dermal regeneration through ang ii in tissue expansion","volume":"24","author":"Bai","year":"2025","unstructured":"Bai, R. et al. Ace2 inhibits dermal regeneration through ang ii in tissue expansion. J Cosmet Dermatol. 24 (1), e16767 (2025).","journal-title":"J Cosmet Dermatol"},{"issue":"19","key":"ref19","doi-asserted-by":"crossref","first-page":"3854","DOI":"10.3390\/polym15193854","type":"journal-article","article-title":"An overview of scaffolds and biomaterials for skin expansion and soft tissue regeneration: Insights on zinc and magnesium as new potential key elements","volume":"15","author":"Hassan","year":"2023","unstructured":"Hassan, N., Krieg, T., Zinser, M., Schr\u00f6der, K., Kr\u00f6ger, N. An overview of scaffolds and biomaterials for skin expansion and soft tissue regeneration: Insights on zinc and magnesium as new potential key elements. Polymers (Basel). 15 (19), 3854 (2023).","journal-title":"Polymers (Basel)"},{"issue":"19","key":"ref20","doi-asserted-by":"crossref","first-page":"3718","DOI":"10.1007\/s00266-024-04132-9","type":"journal-article","article-title":"Aesthetic reconstruction of the sequelae of large facial involuted infantile hemangioma with tissue expanders","volume":"48","author":"Gao","year":"2024","unstructured":"Gao, W. et al. Aesthetic reconstruction of the sequelae of large facial involuted infantile hemangioma with tissue expanders. Aesthetic Plast Surg. 48 (19), 3718-3725 (2024).","journal-title":"Aesthetic Plast Surg"},{"key":"ref21","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202511474","type":"journal-article","article-title":"Mechanical stretch-induced interlayer coordination between mmp2 and col17a1 exacerbates regenerative exhaustion in skin","author":"Sun","year":"2025","unstructured":"Sun, Y. et al. Mechanical stretch-induced interlayer coordination between mmp2 and col17a1 exacerbates regenerative exhaustion in skin. Adv Sci (Weinh). 10.1002\/advs.202511474 e11474 (2025).","journal-title":"Adv Sci (Weinh)"},{"issue":"5","key":"ref22","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1111\/srt.13165","type":"journal-article","article-title":"Characterization and ex vivo evaluation of excised skin samples as substitutes for human dermal barrier in pharmaceutical and dermatological studies","volume":"28","author":"Kocsis","year":"2022","unstructured":"Kocsis, D. et al. Characterization and ex vivo evaluation of excised skin samples as substitutes for human dermal barrier in pharmaceutical and dermatological studies. Skin Res Technol. 28 (5), 664-676 (2022).","journal-title":"Skin Res Technol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"813917","DOI":"10.3389\/fmed.2022.813917","type":"journal-article","article-title":"Metformin promotes mechanical stretch-induced skin regeneration by improving the proliferative activity of skin-derived stem cells","volume":"9","author":"Xiong","year":"2022","unstructured":"Xiong, S. et al. Metformin promotes mechanical stretch-induced skin regeneration by improving the proliferative activity of skin-derived stem cells. Front Med (Lausanne). 9, 813917 (2022).","journal-title":"Front Med (Lausanne)"},{"issue":"3","key":"ref24","doi-asserted-by":"crossref","first-page":"558e","DOI":"10.1097\/PRS.0000000000010753","type":"journal-article","article-title":"Tracing the change and contribution of subcutaneous adipose to skin expansion using a luciferase-transgenic fat transplantation model","volume":"153","author":"Zhou","year":"2024","unstructured":"Zhou, S. B. et al. Tracing the change and contribution of subcutaneous adipose to skin expansion using a luciferase-transgenic fat transplantation model. Plast Reconstr Surg. 153 (3), 558e-567e (2024).","journal-title":"Plast Reconstr Surg"},{"key":"ref25","first-page":"95","type":"journal-article","article-title":"Does the artery of adamkiewicz exist in the albino rat","volume":"161","author":"Schievink","year":"1988","unstructured":"Schievink, W. I., Luyendijk, W., Los, J. A. Does the artery of adamkiewicz exist in the albino rat? J Anat. 161, 95-101 (1988).","journal-title":"J Anat"},{"issue":"162","key":"ref26","first-page":"e61339","type":"journal-article","article-title":"A murine model of a burn wound reconstructed with an allogeneic skin graft","author":"Blaise","year":"2020","unstructured":"Blaise, O., Duchesne, C., Banzet, S., Rousseau, A., Frescaline, N. A murine model of a burn wound reconstructed with an allogeneic skin graft. J Vis Exp. 162, e61339 (2020).","journal-title":"J Vis Exp"},{"issue":"6","key":"ref27","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1016\/j.cvsm.2017.06.007","type":"journal-article","article-title":"Wound closure, tension-relieving techniques, and local flaps","volume":"47","author":"Cuddy","year":"2017","unstructured":"Cuddy, L. C. Wound closure, tension-relieving techniques, and local flaps. Vet Clin North Am Small Anim Pract. 47 (6), 1221-1235 (2017).","journal-title":"Vet Clin North Am Small Anim Pract"},{"issue":"3","key":"ref28","doi-asserted-by":"crossref","first-page":"365","DOI":"10.30802\/AALAS-JAALAS-20-000128","type":"journal-article","article-title":"Using waterless alcohol-based antiseptic for skin preparation and active thermal support in laboratory rats","volume":"60","author":"Hankenson","year":"2021","unstructured":"Hankenson, F. C., Kim, J. J., Le, T. M., Lawrence, F. R., Del Valle, J. M. Using waterless alcohol-based antiseptic for skin preparation and active thermal support in laboratory rats. J Am Assoc Lab Anim Sci. 60 (3), 365-373 (2021).","journal-title":"J Am Assoc Lab Anim Sci"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.actbio.2025.03.026","type":"journal-article","article-title":"Development and calibration of digital twins for human skin growth in tissue expansion","volume":"198","author":"Laudo","year":"2025","unstructured":"Laudo, J. et al. Development and calibration of digital twins for human skin growth in tissue expansion. Acta Biomater. 198, 267-280 (2025).","journal-title":"Acta Biomater"},{"issue":"4","key":"ref30","first-page":"151","type":"journal-article","article-title":"Complications of skin expansion in the pediatric population: A 10-year retrospective study","volume":"3","author":"Hassani","year":"2024","unstructured":"Hassani, Z. A. Fejjal, N. Complications of skin expansion in the pediatric population: A 10-year retrospective study. J Plast Reconstr Surg. 3 (4), 151-156 (2024).","journal-title":"J Plast Reconstr Surg"},{"issue":"34","key":"ref31","doi-asserted-by":"crossref","first-page":"2424713","DOI":"10.1002\/adfm.202424713","type":"journal-article","article-title":"Ultrasound-driven electric conversion hydrogel coating enhances macrophage efferocytosis for non-invasive skin expansion","volume":"35","author":"Zhao","year":"2025","unstructured":"Zhao, Y. et al. Ultrasound-driven electric conversion hydrogel coating enhances macrophage efferocytosis for non-invasive skin expansion. Advanced Functional Materials. 35 (34), 2424713 (2025).","journal-title":"Advanced Functional Materials"},{"issue":"6","key":"ref32","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1097\/PRS.0000000000010891","type":"journal-article","article-title":"Intraoperative indocyanine green angiography facilitates flap fenestration and facial organ fabrication in total facial restoration","volume":"153","author":"Huang","year":"2024","unstructured":"Huang, X. et al. Intraoperative indocyanine green angiography facilitates flap fenestration and facial organ fabrication in total facial restoration. Plast Reconstr Surg. 153 (6), 1416-1424 (2024).","journal-title":"Plast Reconstr Surg"},{"issue":"7","key":"ref33","doi-asserted-by":"crossref","first-page":"e70267","DOI":"10.1111\/jocd.70267","type":"journal-article","article-title":"A clinical early evaluation of the combined use of low-intensity focused ultrasound and radiofrequency for female abdominal contouring","volume":"24","author":"Wu","year":"2025","unstructured":"Wu, Z., Wang, Y., Li, W., Zhang, W., Zhu, L. A clinical early evaluation of the combined use of low-intensity focused ultrasound and radiofrequency for female abdominal contouring. J Cosmet Dermatol. 24 (7), e70267 (2025).","journal-title":"J Cosmet Dermatol"},{"issue":"3","key":"ref34","doi-asserted-by":"crossref","first-page":"e70081","DOI":"10.1111\/exd.70081","type":"journal-article","article-title":"Multimodal second-harmonic-generation, two-photon excitation fluorescence, and brillouin microscopy for visualising dermal mechanical properties in ex vivo human skin","volume":"34","author":"Hase","year":"2025","unstructured":"Hase, E., Okubo, N., Ogura, Y., Minamikawa, T., Yasui, T. Multimodal second-harmonic-generation, two-photon excitation fluorescence, and brillouin microscopy for visualising dermal mechanical properties in ex vivo human skin. Exp Dermatol. 34 (3), e70081 (2025).","journal-title":"Exp Dermatol"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1186\/s13195-025-01841-4","type":"journal-article","article-title":"Disruption of electrophysiological rhythms and memory impairment in an alzheimer's transgenic rat model","volume":"17","author":"Tao","year":"2025","unstructured":"Tao, X. et al. Disruption of electrophysiological rhythms and memory impairment in an alzheimer's transgenic rat model. Alzheimers Res Ther. 17 (1), 200 (2025).","journal-title":"Alzheimers Res Ther"},{"issue":"9","key":"ref36","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1089\/wound.2024.0082","type":"journal-article","article-title":"Comparative analysis of animal models in wound healing research and the utility for humanized mice models","volume":"14","author":"Cirka","year":"2025","unstructured":"Cirka, H. Nguyen, T. T. Comparative analysis of animal models in wound healing research and the utility for humanized mice models. Adv Wound Care (New Rochelle). 14 (9), 479-512 (2025).","journal-title":"Adv Wound Care (New Rochelle)"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/69865\/development-an-efficient-murine-tissue-expansion-model-for-skin","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:18:45Z","timestamp":1792588725000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/69865\/development-an-efficient-murine-tissue-expansion-model-for-skin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,6]]},"references-count":36,"journal-issue":{"issue":"229","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.3791\/69865","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,6]]}}}