{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:47:08Z","timestamp":1792586828686,"version":"4.5.0"},"reference-count":31,"publisher":"MyJove Corporation","issue":"232","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/69983","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T23:00:53Z","timestamp":1782514853000},"source":"Crossref","is-referenced-by-count":0,"title":["A workflow for Live Imaging and Quantitative Analysis of Acentrosomal Microtubule Networks in <em>Drosophila<\/em> Oocytes"],"prefix":"10.3791","author":[{"given":"J\u00e9ssica","family":"Cabrita","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Telmo","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Pimenta-Marques","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"issue":"6","key":"ref1","doi-asserted-by":"crossref","first-page":"a022608","DOI":"10.1101\/cshperspect.a022608","type":"journal-article","article-title":"Microtubules and microtubule-associated proteins","volume":"10","author":"Goodson","year":"2018","unstructured":"Goodson, H. V., Jonasson, E.M. Microtubules and microtubule-associated proteins. Cold Spring Harbor Perspectives in Biology. 10 (6), a022608 (2018).","journal-title":"Cold Spring Harbor Perspectives in Biology"},{"issue":"8","key":"ref2","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/s41580-022-00473-y","type":"journal-article","article-title":"Mechanisms of microtubule organization in differentiated animal cells","volume":"23","author":"Akhmanova","year":"2022","unstructured":"Akhmanova, A., Kapitein, L.C. Mechanisms of microtubule organization in differentiated animal cells. Nature Reviews Molecular Cell Biology. 23 (8), 541\u2013558 (2022).","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.ceb.2016.09.003","type":"journal-article","article-title":"Microtubule-organizing centers: from the centrosome to non-centrosomal sites","volume":"44","author":"Sanchez","year":"2017","unstructured":"Sanchez, A.D., Feldman, J.L. Microtubule-organizing centers: from the centrosome to non-centrosomal sites. Current Opinion in Cell Biology. 44, 93\u2013101 (2017).","journal-title":"Current Opinion in Cell Biology"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1083\/jcb.201704093","type":"journal-article","article-title":"Building the right centriole for each cell type","volume":"217","author":"Loncarek","year":"2018","unstructured":"Loncarek, J., Bettencourt-Dias, M. Building the right centriole for each cell type. The Journal of Cell Biology. 217 (3), 823\u2013835 (2018).","journal-title":"The Journal of Cell Biology"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1534\/genetics.116.198168","type":"journal-article","article-title":"The Centrioles, Centrosomes, Basal Bodies, and Cilia of Drosophila melanogaster","volume":"206","author":"Lattao","year":"2017","unstructured":"Lattao, R., Kov\u00e1cs, L., Glover, D.M. The Centrioles, Centrosomes, Basal Bodies, and Cilia of Drosophila melanogaster. Genetics. 206 (1), 33\u201353 (2017).","journal-title":"Genetics"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1095\/biolreprod.104.031245","type":"journal-article","article-title":"Centrosome reduction during gametogenesis and its significance","volume":"72","author":"Manandhar","year":"2005","unstructured":"Manandhar, G., Schatten, H., Sutovsky, P. Centrosome reduction during gametogenesis and its significance. Biology of reproduction. 72 (1), 2\u201313 (2005).","journal-title":"Biology of reproduction"},{"issue":"11","key":"ref7","doi-asserted-by":"crossref","DOI":"10.1098\/rsob.230222","type":"journal-article","article-title":"Towards understanding centriole elimination","volume":"13","author":"Kalbfuss","year":"2023","unstructured":"Kalbfuss, N., G\u00f6nczy, P. Towards understanding centriole elimination. Open biology. 13 (11) (2023).","journal-title":"Open biology"},{"issue":"17","key":"ref8","doi-asserted-by":"crossref","first-page":"3012","DOI":"10.1242\/dev.153171","type":"journal-article","article-title":"Microtubule organization, dynamics and functions in differentiated cells","volume":"144","author":"Muroyama","year":"2017","unstructured":"Muroyama, A., Lechler, T. Microtubule organization, dynamics and functions in differentiated cells. Development. 144 (17), 3012\u20133021 (2017).","journal-title":"Development"},{"issue":"6294","key":"ref9","doi-asserted-by":"crossref","first-page":"aaf4866","DOI":"10.1126\/science.aaf4866","type":"journal-article","article-title":"A mechanism for the elimination of the female gamete centrosome in Drosophila melanogaster","volume":"353","author":"Pimenta-Marques","year":"2016","unstructured":"Pimenta-Marques, A., Bento, I., Lopes, C.A.M., Duarte, P., Jana, S.C., Bettencourt-Dias, M. A mechanism for the elimination of the female gamete centrosome in Drosophila melanogaster. Science. 353 (6294), aaf4866\u2013aaf4866 (2016).","journal-title":"Science"},{"issue":"10","key":"ref10","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a012294","type":"journal-article","article-title":"mRNA localization and translational control in Drosophila oogenesis","volume":"4","author":"Lasko","year":"2012","unstructured":"Lasko, P. mRNA localization and translational control in Drosophila oogenesis. Cold Spring Harbor Perspectives in Biology. 4 (10) (2012).","journal-title":"Cold Spring Harbor Perspectives in Biology"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1002\/1873-3468.12086","type":"journal-article","article-title":"Shot regulates the microtubule reorganization required for localization of axis-determining mRNAs during oogenesis","volume":"590","author":"Lee","year":"2016","unstructured":"Lee, J., Lee, S., Chen, C., Shim, H., Kim-Ha, J. Shot regulates the microtubule reorganization required for localization of axis-determining mRNAs during oogenesis. FEBS Letters. 590 (4), 431\u2013444 (2016).","journal-title":"FEBS Letters"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.devcel.2016.06.010","type":"journal-article","article-title":"Patronin\/Shot Cortical Foci Assemble the Noncentrosomal Microtubule Array that Specifies the Drosophila Anterior-Posterior Axis","volume":"38","author":"Nashchekin","year":"2016","unstructured":"Nashchekin, D., Fernandes, A.R., St Johnston, D. Patronin\/Shot Cortical Foci Assemble the Noncentrosomal Microtubule Array that Specifies the Drosophila Anterior-Posterior Axis. Developmental Cell. 38 (1), 61\u201372 (2016).","journal-title":"Developmental Cell"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.tcb.2020.10.004","type":"journal-article","article-title":"Cutting, Amplifying, and Aligning Microtubules with Severing Enzymes","volume":"31","author":"Kuo","year":"2021","unstructured":"Kuo, Y.W., Howard, J. Cutting, Amplifying, and Aligning Microtubules with Severing Enzymes. Trends in cell biology. 31 (1), 50\u201361 (2021).","journal-title":"Trends in cell biology"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1083\/jcb.201103160","type":"journal-article","article-title":"A PAR-1\u2013dependent orientation gradient of dynamic microtubules directs posterior cargo transport in the Drosophila oocyte","volume":"194","author":"Parton","year":"2011","unstructured":"Parton, R.M. et al. A PAR-1\u2013dependent orientation gradient of dynamic microtubules directs posterior cargo transport in the Drosophila oocyte. Journal of Cell Biology. 194 (1), 121\u2013135 (2011).","journal-title":"Journal of Cell Biology"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.27237","type":"journal-article","article-title":"Localised dynactin protects growing microtubules to deliver oskar mRNA to the posterior cortex of the Drosophila oocyte","volume":"6","author":"Nieuwburg","year":"2017","unstructured":"Nieuwburg, R. et al. Localised dynactin protects growing microtubules to deliver oskar mRNA to the posterior cortex of the Drosophila oocyte. eLife. 6 (2017).","journal-title":"eLife"},{"issue":"39","key":"ref16","doi-asserted-by":"crossref","first-page":"e2303376120","DOI":"10.1073\/pnas.2303376120","type":"journal-article","article-title":"The dynamic duo of microtubule polymerase Mini spindles\/XMAP215 and cytoplasmic dynein is essential for maintaining Drosophila oocyte fate","volume":"120","author":"Lu","year":"2023","unstructured":"Lu, W., Lakonishok, M., Gelfand, V.I. The dynamic duo of microtubule polymerase Mini spindles\/XMAP215 and cytoplasmic dynein is essential for maintaining Drosophila oocyte fate. Proceedings of the National Academy of Sciences. 120 (39), e2303376120 (2023).","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"5","key":"ref17","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1083\/jcb.200807179","type":"journal-article","article-title":"Mammalian end binding proteins control persistent microtubule growth","volume":"184","author":"Komarova","year":"2009","unstructured":"Komarova, Y. et al. Mammalian end binding proteins control persistent microtubule growth. The Journal of cell biology. 184 (5), 691\u2013706 (2009).","journal-title":"The Journal of cell biology"},{"issue":"5","key":"ref18","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1083\/jcb.200202032","type":"journal-article","article-title":"Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle","volume":"158","author":"Rogers","year":"2002","unstructured":"Rogers, S.L., Rogers, G.C., Sharp, D.J., Vale, R.D. Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle. The Journal of cell biology. 158 (5), 873\u2013884 (2002).","journal-title":"The Journal of cell biology"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.devcel.2007.01.021","type":"journal-article","article-title":"Functionally Unequal Centrosomes Drive Spindle Orientation in Asymmetrically Dividing Drosophila Neural Stem Cells","volume":"12","author":"Rebollo","year":"2007","unstructured":"Rebollo, E., Sampaio, P., Januschke, J., Llamazares, S., Varmark, H., Gonz\u00e1lez, C. Functionally Unequal Centrosomes Drive Spindle Orientation in Asymmetrically Dividing Drosophila Neural Stem Cells. Developmental Cell. 12 (3), 467\u2013474 (2007).","journal-title":"Developmental Cell"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/978-1-4939-2851-4_7","type":"journal-article","article-title":"Visualizing microtubule networks during Drosophila oogenesis using fixed and live imaging","author":"Legent","year":"2015","unstructured":"Legent, K., Tissot, N., Guichet, A. Visualizing microtubule networks during Drosophila oogenesis using fixed and live imaging. Drosophila Oogenesis: Methods and Protocols. 99\u2013112 (2015).","journal-title":"Drosophila Oogenesis: Methods and Protocols"},{"issue":"4","key":"ref21","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1006\/meth.1998.0592","type":"journal-article","article-title":"Ectopic gene expression in Drosophila using GAL4 system","volume":"14","author":"Phelps","year":"1998","unstructured":"Phelps, C.B., Brand, A.H. Ectopic gene expression in Drosophila using GAL4 system. Methods: A Companion to Methods in Enzymology. 14 (4), 367\u2013379 (1998).","journal-title":"Methods: A Companion to Methods in Enzymology"},{"issue":"60","key":"ref22","doi-asserted-by":"crossref","DOI":"10.3791\/3679-v","type":"journal-article","article-title":"Preparing individual Drosophila egg chambers for live imaging","author":"Weil","year":"2012","unstructured":"Weil, T.T., Parton, R.M., Davis, I. Preparing individual Drosophila egg chambers for live imaging. JoVE. (60) (2012).","journal-title":"JoVE"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1242\/dev.02179","type":"journal-article","article-title":"The centrosome-nucleus complex and microtubule organization in the Drosophila oocyte","volume":"133","author":"Januschke","year":"2006","unstructured":"Januschke, J., Gervais, L., Gillet, L., Keryer, G., Bornens, M., Guichet, A. The centrosome-nucleus complex and microtubule organization in the Drosophila oocyte. Development. 133 (1), 129\u2013139 (2006).","journal-title":"Development"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"18850","DOI":"10.1038\/srep18850","type":"journal-article","article-title":"Automatic stage identification of Drosophila egg chamber based on DAPI images","volume":"6","author":"Jia","year":"2016","unstructured":"Jia, D., Xu, Q., Xie, Q., Mio, W., Deng, W.M. Automatic stage identification of Drosophila egg chamber based on DAPI images. Scientific Reports .6 (1), 18850 (2016).","journal-title":"Scientific Reports"},{"issue":"7","key":"ref25","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1038\/s41592-022-01507-1","type":"journal-article","article-title":"TrackMate 7: integrating state-of-the-art segmentation algorithms into tracking pipelines","volume":"19","author":"Ershov","year":"2022","unstructured":"Ershov, D. et al. TrackMate 7: integrating state-of-the-art segmentation algorithms into tracking pipelines. Nature methods. 19 (7), 829\u2013832 (2022).","journal-title":"Nature methods"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1534\/genetics.104.026427","type":"journal-article","article-title":"The BDGP gene disruption project: Single transposon insertions associated with 40% of Drosophila genes","volume":"167","author":"Bellen","year":"2004","unstructured":"Bellen, H.J. et al. The BDGP gene disruption project: Single transposon insertions associated with 40% of Drosophila genes. Genetics. 167 (2), 761\u2013781 (2004).","journal-title":"Genetics"},{"issue":"2","key":"ref27","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.cell.2010.09.022","type":"journal-article","article-title":"Patronin regulates the microtubule network by protecting microtubule minus ends","volume":"143","author":"Goodwin","year":"2010","unstructured":"Goodwin, S.S., Vale, R.D. Patronin regulates the microtubule network by protecting microtubule minus ends. Cell. 143 (2), 263\u2013274 (2010).","journal-title":"Cell"},{"key":"ref28","type":"journal-article","article-title":"Spatial activation of Kinesin-1 by Ensconsin shapes microtubule networks via ncMTOCs recruitment","author":"Berisha","year":"2025","unstructured":"Berisha AM, et al. Spatial activation of Kinesin-1 by Ensconsin shapes microtubule networks via ncMTOCs recruitment. bioRxiv. 2025 Apr 16:2025-04. (Pre-print) (2025).","journal-title":"bioRxiv"},{"issue":"3","key":"ref29","first-page":"e56624","type":"journal-article","article-title":"Patronin\/CAMSAP promotes reactivation and regeneration of Drosophila quiescent neural stem cells","volume":"24","author":"Gujar","year":"2023","unstructured":"Gujar, M. R. et al. Patronin\/CAMSAP promotes reactivation and regeneration of Drosophila quiescent neural stem cells. EMBO Reports. 24 (3), e56624 (2023).","journal-title":"EMBO Reports"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1109\/JRPROC.1949.232969","type":"journal-article","article-title":"Communication in the Presence of Noise","volume":"37","author":"Shannon","year":"1949","unstructured":"Shannon, C.E. Communication in the Presence of Noise. Proceedings of the IRE. 37 (1), 10\u201321 (1949).","journal-title":"Proceedings of the IRE"},{"issue":"10","key":"ref31","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1007\/s00018-016-2168-3","type":"journal-article","article-title":"Microtubule plus-end tracking proteins in neuronal development","volume":"73","author":"van de Willige","year":"2016","unstructured":"van de Willige, D., Hoogenraad, C.C., Akhmanova, A., Hoogenraad choogenraad, C.C., Akhmanova aakhmanova, A. Microtubule plus-end tracking proteins in neuronal development. Cellular and Molecular Life Sciences 2016 73:10. 73 (10), 2053\u20132077 (2016).","journal-title":"Cellular and Molecular Life Sciences"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/69983\/a-workflow-for-live-imaging-quantitative-analysis-acentrosomal","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:26:12Z","timestamp":1792585572000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/69983\/a-workflow-for-live-imaging-quantitative-analysis-acentrosomal"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,26]]},"references-count":31,"journal-issue":{"issue":"232","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.3791\/69983","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,26]]}}}