{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:49:14Z","timestamp":1792586954026,"version":"4.5.0"},"reference-count":18,"publisher":"MyJove Corporation","issue":"232","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/70509","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T23:02:32Z","timestamp":1780700552000},"source":"Crossref","is-referenced-by-count":0,"title":["Validation of Random Transgene Integration and Expression in <em>Eimeria<\/em> Parasites"],"prefix":"10.3791","author":[{"given":"Yingying","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuehui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianyong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxin","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Suo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2026,6,5]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"105130","DOI":"10.1016\/j.psj.2025.105130","type":"journal-article","article-title":"Feeding the future: A new potential nutritional impact of Lactiplantibacillus plantarum and its promising interventions in future for poultry industry","volume":"104","author":"Saeed","year":"2025","unstructured":"Saeed M, et al. Feeding the future: A new potential nutritional impact of Lactiplantibacillus plantarum and its promising interventions in future for poultry industry. Poult Sci. 2025;104:105130.","journal-title":"Poult Sci"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/s13567-020-00837-2","type":"journal-article","article-title":"Re-calculating the cost of coccidiosis in chickens","volume":"51","author":"Blake","year":"2020","unstructured":"Blake DP, et al. Re-calculating the cost of coccidiosis in chickens. Vet Res. 2020;51(1):115.","journal-title":"Vet Res"},{"issue":"1-2","key":"ref3","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.vetpar.2006.06.020","type":"journal-article","article-title":"The efficacy and economic benefits of Supercox, a live anticoccidial vaccine in a commercial trial in broiler chickens in China","volume":"142","author":"Suo","year":"2006","unstructured":"Suo X, et al. The efficacy and economic benefits of Supercox, a live anticoccidial vaccine in a commercial trial in broiler chickens in China. Vet Parasitol. 2006;142(1-2):63-70.","journal-title":"Vet Parasitol"},{"issue":"12","key":"ref4","doi-asserted-by":"crossref","first-page":"e114188","DOI":"10.1371\/journal.pone.0114188","type":"journal-article","article-title":"Transfection of Eimeria mitis with yellow fluorescent protein as reporter and the endogenous development of the transgenic parasite","volume":"9","author":"Qin","year":"2014","unstructured":"Qin M, et al. Transfection of Eimeria mitis with yellow fluorescent protein as reporter and the endogenous development of the transgenic parasite. PLoS One. 2014;9(12):e114188.","journal-title":"PLoS One"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.parint.2012.01.010","type":"journal-article","article-title":"Chimeric fluorescent reporter as a tool for generation of transgenic Eimeria (Apicomplexa, Coccidia) strains with stage-specific reporter gene expression","volume":"61","author":"Hanig","year":"2012","unstructured":"Hanig S, et al. Chimeric fluorescent reporter as a tool for generation of transgenic Eimeria (Apicomplexa, Coccidia) strains with stage-specific reporter gene expression. Parasitol Int. 2012;61(3):391-398.","journal-title":"Parasitol Int"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"29379","DOI":"10.1038\/srep29379","type":"journal-article","article-title":"Transgenic Eimeria tenella as a vaccine vehicle: Expressing TgSAG1 elicits protective immunity against Toxoplasma gondii infections in chickens and mice","volume":"6","author":"Tang","year":"2016","unstructured":"Tang XM, et al. Transgenic Eimeria tenella as a vaccine vehicle: Expressing TgSAG1 elicits protective immunity against Toxoplasma gondii infections in chickens and mice. Sci Rep. 2016;6:29379.","journal-title":"Sci Rep"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.molbiopara.2008.07.006","type":"journal-article","article-title":"A toolbox facilitating stable transfection of Eimeria species","volume":"162","author":"Clark","year":"2008","unstructured":"Clark JD, et al. A toolbox facilitating stable transfection of Eimeria species. Mol Biochem Parasitol. 2008;162(1):77-86.","journal-title":"Mol Biochem Parasitol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"105417","DOI":"10.1016\/j.actatropica.2020.105417","type":"journal-article","article-title":"Transgenic Eimeria parasite: A potential control strategy for chicken coccidiosis","volume":"205","author":"Fatoba","year":"2020","unstructured":"Fatoba AJ, Adeleke MA. Transgenic Eimeria parasite: A potential control strategy for chicken coccidiosis. Acta Trop. 2020;205:105417.","journal-title":"Acta Trop"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"127715","DOI":"10.1016\/j.vaccine.2025.127715","type":"journal-article","article-title":"Immunogenicity and protection against infectious bursal disease via a transgenic Eimeria acervulina expressing IBDV VP2-2C3d fusion protein","volume":"64","author":"Sun","year":"2025","unstructured":"Sun YY, et al. Immunogenicity and protection against infectious bursal disease via a transgenic Eimeria acervulina expressing IBDV VP2-2C3d fusion protein. Vaccine. 2025;64:127715.","journal-title":"Vaccine"},{"issue":"156","key":"ref10","first-page":"e156","type":"journal-article","article-title":"Nucleofection and in vivo propagation of chicken Eimeria parasites","author":"Duan","year":"2020","unstructured":"Duan CH, et al. Nucleofection and in vivo propagation of chicken Eimeria parasites. J Vis Exp. 2020;(156):e156.","journal-title":"J Vis Exp"},{"issue":"2","key":"ref11","first-page":"1008","type":"journal-article","article-title":"Construction and identification of transgenic Eimeria tenella expressing IBDV VP2 protein","volume":"57","author":"Liu","year":"2026","unstructured":"Liu YH, et al. Construction and identification of transgenic Eimeria tenella expressing IBDV VP2 protein. J Anim Sci Vet Med. 2026;57(2):1008-1016.","journal-title":"J Anim Sci Vet Med"},{"issue":"17","key":"ref12","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","type":"journal-article","article-title":"Fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","unstructured":"Chen SF, et al. Fastp: An ultra-fast all-in-one FASTQ preprocessor. Bioinformatics. 2018;34(17):i884-i890.","journal-title":"Bioinformatics"},{"issue":"4","key":"ref13","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","type":"journal-article","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","unstructured":"Langmead B, Salzberg SL. Fast gapped-read alignment with Bowtie 2. Nat Methods. 2012;9(4):357-359.","journal-title":"Nat Methods"},{"issue":"5","key":"ref14","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1093\/bioinformatics\/btx699","type":"journal-article","article-title":"Mosdepth: Quick coverage calculation for genomes and exomes","volume":"34","author":"Pedersen","year":"2018","unstructured":"Pedersen BS, Quinlan AR. Mosdepth: Quick coverage calculation for genomes and exomes. Bioinformatics. 2018;34(5):867-868.","journal-title":"Bioinformatics"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"106238","DOI":"10.1016\/j.psj.2025.106238","type":"journal-article","article-title":"Dual-copy VP2 expressed with CTA1-DD in transgenic Eimeria acervulina confers partial protection against infectious bursal disease virus","volume":"105","author":"Sun","year":"2026","unstructured":"Sun YY, et al. Dual-copy VP2 expressed with CTA1-DD in transgenic Eimeria acervulina confers partial protection against infectious bursal disease virus. Poult Sci. 2026;105(2):106238.","journal-title":"Poult Sci"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1367912","DOI":"10.3389\/fvets.2024.1367912","type":"journal-article","article-title":"Oral delivery of Eimeria acervulina transfected sequentially with two copies of the VP2 gene induces immunity against infectious bursal disease virus in chickens","volume":"11","author":"Guo","year":"2024","unstructured":"Guo QB, et al. Oral delivery of Eimeria acervulina transfected sequentially with two copies of the VP2 gene induces immunity against infectious bursal disease virus in chickens. Front Vet Sci. 2024;11:1367912.","journal-title":"Front Vet Sci"},{"issue":"7","key":"ref17","doi-asserted-by":"crossref","first-page":"791","DOI":"10.3390\/vaccines9070791","type":"journal-article","article-title":"Establishment of recombinant Eimeria acervulina expressing multi-copies M2e derived from avian influenza virus H9N2","volume":"9","author":"Zhang","year":"2021","unstructured":"Zhang SX, et al. Establishment of recombinant Eimeria acervulina expressing multi-copies M2e derived from avian influenza virus H9N2. Vaccines (Basel). 2021;9(7):791.","journal-title":"Vaccines (Basel)"},{"issue":"3","key":"ref18","doi-asserted-by":"crossref","first-page":"112060","DOI":"10.1016\/j.isci.2025.112060","type":"journal-article","article-title":"Genetic manipulation for the non-model protozoan Eimeria: Advancements, challenges, and future perspective","volume":"28","author":"Li","year":"2025","unstructured":"Li YR, et al. Genetic manipulation for the non-model protozoan Eimeria: Advancements, challenges, and future perspective. iScience. 2025;28(3):112060.","journal-title":"iScience"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/70509\/validation-random-transgene-integration-expression-eimeria","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:34:05Z","timestamp":1792586045000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/70509\/validation-random-transgene-integration-expression-eimeria"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,5]]},"references-count":18,"journal-issue":{"issue":"232","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.3791\/70509","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,5]]}}}