{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:46:40Z","timestamp":1792586800727,"version":"4.5.0"},"reference-count":16,"publisher":"MyJove Corporation","issue":"232","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/70687","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T23:03:57Z","timestamp":1782860637000},"source":"Crossref","is-referenced-by-count":0,"title":["Establishing An Epithelial-Endothelial Co-Culture Lung-on-a-chip For Investigation In Healthy and COPD Conditions"],"prefix":"10.3791","author":[{"given":"Lucia","family":"Aversa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janne","family":"Verhaegen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn","family":"Willems","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Geudens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ph\u00e9line","family":"Kortleven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Mercurio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela E.","family":"Cortesi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjolein M.","family":"Ensinck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celine","family":"Aelbrecht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne S.","family":"Carlon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rozenn","family":"Quarck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart M.","family":"Vanaudenaerde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghislaine","family":"Gayan-Ramirez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","DOI":"10.1002\/iid3.139","type":"journal-article","article-title":"Cellular crosstalk between airway epithelial and endothelial cells regulates barrier functions during exposure to double-stranded RNA","author":"Blume","year":"2017","unstructured":"Blume C, et al. Cellular crosstalk between airway epithelial and endothelial cells regulates barrier functions during exposure to double-stranded RNA. Immun Inflamm Dis. 2017;5(1):45\u201356.","journal-title":"Immun Inflamm Dis"},{"key":"ref2","doi-asserted-by":"crossref","DOI":"10.1183\/09059180.00007913","type":"journal-article","article-title":"The vascular bed in COPD: pulmonary hypertension and pulmonary vascular alterations","author":"Sakao","year":"2014","unstructured":"Sakao S, Voelkel NF, Tatsumi K. The vascular bed in COPD: pulmonary hypertension and pulmonary vascular alterations. Eur Respir Rev. 2014;23(133):350\u2013355.","journal-title":"Eur Respir Rev"},{"key":"ref3","doi-asserted-by":"crossref","DOI":"10.1513\/AnnalsATS.201402-049AW","type":"journal-article","article-title":"Early events in the pathogenesis of chronic obstructive pulmonary disease: smoking-induced reprogramming of airway epithelial basal progenitor cells","author":"Shaykhiev","year":"2014","unstructured":"Shaykhiev R, Crystal RG. Early events in the pathogenesis of chronic obstructive pulmonary disease: smoking-induced reprogramming of airway epithelial basal progenitor cells. Ann Am Thorac Soc. 2014;11(Suppl 5):S252\u2013S258.","journal-title":"Ann Am Thorac Soc"},{"key":"ref4","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejpb.2012.12.014","type":"journal-article","article-title":"In vitro and ex vivo models of human asthma","author":"Blume","year":"2013","unstructured":"Blume C, Davies DE. In vitro and ex vivo models of human asthma. Eur J Pharm Biopharm. 2013;84(2):394\u2013400.","journal-title":"Eur J Pharm Biopharm"},{"key":"ref5","type":"journal-article","article-title":"Mechanotransduction regulates the interplays between alveolar epithelial and vascular endothelial cells in lung","author":"Lin","year":"2022","unstructured":"Lin C, et al. Mechanotransduction regulates the interplays between alveolar epithelial and vascular endothelial cells in lung. Front Physiol. 2022;13:818394.","journal-title":"Front Physiol"},{"key":"ref6","doi-asserted-by":"crossref","DOI":"10.1080\/07388551.2019.1710458","type":"journal-article","article-title":"Lung-on-a-chip: the future of respiratory disease models and pharmacological studies","author":"Shrestha","year":"2020","unstructured":"Shrestha J, et al. Lung-on-a-chip: the future of respiratory disease models and pharmacological studies. Crit Rev Biotechnol. 2020;40(2):213\u2013230.","journal-title":"Crit Rev Biotechnol"},{"key":"ref7","doi-asserted-by":"crossref","DOI":"10.1038\/nmeth.3697","type":"journal-article","article-title":"Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro","author":"Benam","year":"2016","unstructured":"Benam KH, et al. Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro. Nat Methods. 2016;13(2):151\u2013157.","journal-title":"Nat Methods"},{"key":"ref8","doi-asserted-by":"crossref","DOI":"10.1016\/j.cels.2016.10.003","type":"journal-article","article-title":"Matched-comparative modeling of normal and diseased human airway responses using a microengineered breathing lung chip","author":"Benam","year":"2016","unstructured":"Benam KH, et al. Matched-comparative modeling of normal and diseased human airway responses using a microengineered breathing lung chip. Cell Syst. 2016;3(5):456\u2013466.e4.","journal-title":"Cell Syst"},{"key":"ref9","doi-asserted-by":"crossref","DOI":"10.1016\/j.jcf.2021.10.004","type":"journal-article","article-title":"Modeling pulmonary cystic fibrosis in a human lung airway-on-a-chip","author":"Plebani","year":"2022","unstructured":"Plebani R, et al. Modeling pulmonary cystic fibrosis in a human lung airway-on-a-chip. J Cyst Fibros. 2022;21(4):606\u2013615.","journal-title":"J Cyst Fibros"},{"key":"ref10","doi-asserted-by":"crossref","DOI":"10.1126\/science.1188302","type":"journal-article","article-title":"Reconstituting organ-level lung functions on a chip","author":"Huh","year":"2010","unstructured":"Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662\u20131668.","journal-title":"Science"},{"key":"ref11","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2199-7-3","type":"journal-article","article-title":"The RIN: an RNA integrity number for assigning integrity values to RNA measurements","author":"Schroeder","year":"2006","unstructured":"Schroeder A, et al. The RIN: an RNA integrity number for assigning integrity values to RNA measurements. BMC Mol Biol. 2006;7:3.","journal-title":"BMC Mol Biol"},{"key":"ref12","doi-asserted-by":"crossref","DOI":"10.1039\/C4LC01252F","type":"journal-article","article-title":"A lung-on-a-chip array with an integrated bio-inspired respiration mechanism","author":"Stucki","year":"2015","unstructured":"Stucki AO, et al. A lung-on-a-chip array with an integrated bio-inspired respiration mechanism. Lab Chip. 2015;15(5):1302\u20131310.","journal-title":"Lab Chip"},{"key":"ref13","doi-asserted-by":"crossref","DOI":"10.1183\/13993003.01805-2016","type":"journal-article","article-title":"The instructive extracellular matrix of the lung","author":"Burgstaller","year":"2017","unstructured":"Burgstaller G, et al. The instructive extracellular matrix of the lung. Eur Respir J. 2017;50(1):1601805.","journal-title":"Eur Respir J"},{"key":"ref14","doi-asserted-by":"crossref","DOI":"10.1177\/2211068214561025","type":"journal-article","article-title":"TEER measurement techniques for in vitro barrier model systems","author":"Srinivasan","year":"2015","unstructured":"Srinivasan B, et al. TEER measurement techniques for in vitro barrier model systems. J Lab Autom. 2015;20(2):107\u2013126.","journal-title":"J Lab Autom"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.1002\/cphy.c180020","type":"journal-article","article-title":"Mechanosensing and mechanoregulation of endothelial cell functions","author":"Fang","year":"2019","unstructured":"Fang Y, Wu D, Birukov KG. Mechanosensing and mechanoregulation of endothelial cell functions. Compr Physiol. 2019;9(2):873\u2013904.","journal-title":"Compr Physiol"},{"key":"ref16","type":"journal-article","article-title":"The airways' mechanical stress in lung disease: implications for COPD pathophysiology and treatment evaluation","author":"Santus","year":"2019","unstructured":"Santus P, et al. The airways' mechanical stress in lung disease: implications for COPD pathophysiology and treatment evaluation. Can Respir J. 2019;2019:3546056.","journal-title":"Can Respir J"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/70687\/establishing-an-epithelial-endothelial-co-culture-lung-on-chip-for","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:24:24Z","timestamp":1792585464000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/70687\/establishing-an-epithelial-endothelial-co-culture-lung-on-chip-for"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,30]]},"references-count":16,"journal-issue":{"issue":"232","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.3791\/70687","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,30]]}}}