{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:46:20Z","timestamp":1792586780899,"version":"4.5.0"},"reference-count":23,"publisher":"MyJove Corporation","issue":"233","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/70704","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T23:02:26Z","timestamp":1783119746000},"source":"Crossref","is-referenced-by-count":1,"title":["Cervical Heterotopic Mouse Heart Transplantation using End-to-side Anastomosis Technique to Study Transplant Immunology"],"prefix":"10.3791","author":[{"given":"Xia","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingbin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linrui","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zipei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2026,7,3]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.688460","type":"journal-article","article-title":"Harnessing mechanisms of immune tolerance to improve outcomes in solid organ transplantation: a review","author":"Slepicka","year":"2021","unstructured":"Slepicka PF, Yazdanifar M, Bertaina A. Harnessing mechanisms of immune tolerance to improve outcomes in solid organ transplantation: a review. Front Immunol. (2021).","journal-title":"Front Immunol"},{"key":"ref2","doi-asserted-by":"crossref","DOI":"10.1016\/j.ajt.2024.01.006","type":"journal-article","article-title":"Advancing mouse models for transplantation research","author":"Cravedi","year":"2024","unstructured":"Cravedi P et al. Advancing mouse models for transplantation research. Am J Transplant Off J Am Soc Transplant Am Soc Transpl Surg. (2024).","journal-title":"Am J Transplant Off J Am Soc Transplant Am Soc Transpl Surg"},{"key":"ref3","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejphar.2015.03.025","type":"journal-article","article-title":"The utility of animal models in developing immunosuppressive agents","author":"McDaid","year":"2015","unstructured":"McDaid J et al. The utility of animal models in developing immunosuppressive agents. Eur J Pharmacol. (2015).","journal-title":"Eur J Pharmacol"},{"key":"ref4","type":"journal-article","article-title":"Efficacy of single-agent immunosuppressive regimens in a murine model of vascularized composite allotransplantation","author":"Guo","year":"2020","unstructured":"Guo Y et al. Efficacy of single-agent immunosuppressive regimens in a murine model of vascularized composite allotransplantation. Transpl Int Off J Eur Soc Organ Transplant. (2020).","journal-title":"Transpl Int Off J Eur Soc Organ Transplant"},{"key":"ref5","doi-asserted-by":"crossref","DOI":"10.1016\/j.ajt.2025.04.012","type":"journal-article","article-title":"Best practices of heart transplantation in mice","author":"Alegre","year":"2025","unstructured":"Alegre M-L et al. Best practices of heart transplantation in mice. Am J Transplant Off J Am Soc Transplant Am Soc Transpl Surg. (2025).","journal-title":"Am J Transplant Off J Am Soc Transplant Am Soc Transpl Surg"},{"key":"ref6","doi-asserted-by":"crossref","DOI":"10.3109\/08941939.2012.755238","type":"journal-article","article-title":"Four decades of vascularized heterotopic cardiac transplantation in the mouse","author":"Plenter","year":"2013","unstructured":"Plenter RJ, Zamora MR, Grazia TJ. Four decades of vascularized heterotopic cardiac transplantation in the mouse. J Invest Surg. (2013).","journal-title":"J Invest Surg"},{"key":"ref7","type":"journal-article","article-title":"Heart transplantation in congenic strains of mice","author":"Corry","year":"1973","unstructured":"Corry RJ, Winn HJ, Russell PS. Heart transplantation in congenic strains of mice. Transplant Proc. (1973).","journal-title":"Transplant Proc"},{"key":"ref8","doi-asserted-by":"crossref","DOI":"10.1097\/00007890-199112000-00035","type":"journal-article","article-title":"A technique of cervical heterotopic heart transplantation in mice","author":"Chen","year":"1991","unstructured":"Chen ZH. A technique of cervical heterotopic heart transplantation in mice. Transplantation. (1991).","journal-title":"Transplantation"},{"key":"ref9","type":"journal-article","article-title":"Simplified mouse cervical heart transplantation using a cuff technique","author":"Matsuura","year":"1991","unstructured":"Matsuura A, Abe T, Yasuura K. Simplified mouse cervical heart transplantation using a cuff technique. Transplantation. (1991).","journal-title":"Transplantation"},{"key":"ref10","doi-asserted-by":"crossref","DOI":"10.1097\/TP.0000000000002395","type":"journal-article","article-title":"Submandibular gland-preserving technique for heterotopic cervical heart transplantation in mice","author":"Fukunaga","year":"2018","unstructured":"Fukunaga N, Bissoondath V, Rao V. Submandibular gland-preserving technique for heterotopic cervical heart transplantation in mice. Transplantation. (2018).","journal-title":"Transplantation"},{"key":"ref11","doi-asserted-by":"crossref","DOI":"10.1097\/00007890-900000000-96265","type":"journal-article","article-title":"Custom-made clamp facilitating stabilization of cuffs in murine heterotopic cervical heart transplantation","author":"Fukunaga","year":"2019","unstructured":"Fukunaga N, Bissoondath V, Rao V. Custom-made clamp facilitating stabilization of cuffs in murine heterotopic cervical heart transplantation. Transplantation. (2019).","journal-title":"Transplantation"},{"key":"ref12","type":"journal-article","article-title":"Cuff-free suture-based cervical model for secondary heterotopic heart transplantation to evaluate donor-specific immune tolerance in mice","author":"Liao","year":"2026","unstructured":"Liao M, Liu K, Riella LV, Borges TJ. Cuff-free suture-based cervical model for secondary heterotopic heart transplantation to evaluate donor-specific immune tolerance in mice. J Vis Exp JoVE. (2026).","journal-title":"J Vis Exp JoVE"},{"key":"ref13","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijdevneu.2008.12.006","type":"journal-article","article-title":"Impact of age and strain on ischemic brain injury and seizures after carotid ligation in immature mice","author":"Comi","year":"2009","unstructured":"Comi AM et al. Impact of age and strain on ischemic brain injury and seizures after carotid ligation in immature mice. Int J Dev Neurosci. (2009).","journal-title":"Int J Dev Neurosci"},{"key":"ref14","doi-asserted-by":"crossref","DOI":"10.3791\/52907","type":"journal-article","article-title":"Heterotopic cervical heart transplantation in mice","author":"Ratschiller","year":"2015","unstructured":"Ratschiller T et al. Heterotopic cervical heart transplantation in mice. J Vis Exp Jove. (2015).","journal-title":"J Vis Exp Jove"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.1177\/0271678X16638349","type":"journal-article","article-title":"In vivo evidence for long-term vascular remodeling resulting from chronic cerebral hypoperfusion in mice","author":"Struys","year":"2017","unstructured":"Struys T et al. In vivo evidence for long-term vascular remodeling resulting from chronic cerebral hypoperfusion in mice. J Cereb Blood Flow Metab Off J Int Soc Cereb Blood Flow Metab. (2017).","journal-title":"J Cereb Blood Flow Metab Off J Int Soc Cereb Blood Flow Metab"},{"key":"ref16","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhlto.2024.100164","type":"journal-article","article-title":"Cervical heterotopic heart transplantation in mice using a novel suture technique","author":"Dun","year":"2025","unstructured":"Dun H et al. Cervical heterotopic heart transplantation in mice using a novel suture technique. JHLT Open. (2025).","journal-title":"JHLT Open"},{"key":"ref17","type":"journal-article","article-title":"Mouse heterotopic cervical cardiac transplantation utilizing vascular cuffs","author":"Li","year":"2022","unstructured":"Li W et al. Mouse heterotopic cervical cardiac transplantation utilizing vascular cuffs. J Vis Exp. (2022).","journal-title":"J Vis Exp"},{"key":"ref18","doi-asserted-by":"crossref","DOI":"10.1097\/01.prs.0000437229.69811.3a","type":"journal-article","article-title":"The neck as a preferred recipient site for vascularized composite allotransplantation in the mouse","author":"Lin","year":"2014","unstructured":"Lin C-H et al. The neck as a preferred recipient site for vascularized composite allotransplantation in the mouse: Plast Reconstr Surg. (2014).","journal-title":"Plast Reconstr Surg"},{"key":"ref19","doi-asserted-by":"crossref","DOI":"10.1002\/micr.20082","type":"journal-article","article-title":"Simplified technique for heterotopic vascularized cervical heart transplantation in mice","author":"Wang","year":"2005","unstructured":"Wang Q, Liu Y, Li X-K. Simplified technique for heterotopic vascularized cervical heart transplantation in mice. Microsurgery. (2005).","journal-title":"Microsurgery"},{"key":"ref20","doi-asserted-by":"crossref","DOI":"10.1002\/micr.20363","type":"journal-article","article-title":"Improved cuff technique for cervical heart transplantation in mice","author":"Gu","year":"2007","unstructured":"Gu X et al. Improved cuff technique for cervical heart transplantation in mice. Microsurgery. (2007).","journal-title":"Microsurgery"},{"key":"ref21","doi-asserted-by":"crossref","DOI":"10.3791\/50753-v","type":"journal-article","article-title":"Murine cervical heart transplantation model using a modified cuff technique","author":"Oberhuber","year":"2014","unstructured":"Oberhuber R et al. Murine cervical heart transplantation model using a modified cuff technique. J Vis Exp. (2014).","journal-title":"J Vis Exp"},{"key":"ref22","doi-asserted-by":"crossref","DOI":"10.1111\/xen.12331","type":"journal-article","article-title":"A highly reproducible cervical cuff technique for rat-to-mouse heterotopic heart xenotransplantation","author":"Li","year":"2017","unstructured":"Li Y et al. A highly reproducible cervical cuff technique for rat-to-mouse heterotopic heart xenotransplantation. Xenotransplantation. (2017).","journal-title":"Xenotransplantation"},{"key":"ref23","type":"journal-article","article-title":"Comparison of 2 heterotopic heart transplant techniques in rats: cervical and abdominal heart","author":"Ma","year":"2011","unstructured":"Ma Y, Wang G. Comparison of 2 heterotopic heart transplant techniques in rats: cervical and abdominal heart. Exp Clin Transplant Off J Middle East Soc Organ Transplant. (2011).","journal-title":"Exp Clin Transplant Off J Middle East Soc Organ Transplant"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/70704\/cervical-heterotopic-mouse-heart-transplantation-using-end-to-side","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:23:04Z","timestamp":1792585384000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jove.com\/t\/70704\/cervical-heterotopic-mouse-heart-transplantation-using-end-to-side"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,3]]},"references-count":23,"journal-issue":{"issue":"233","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.3791\/70704","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,3]]}}}