{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:44:54Z","timestamp":1792586694396,"version":"4.5.0"},"reference-count":0,"publisher":"The Journal of Food and Drug Analysis (JFDA), Food and Drug Administration, Taiwan (TFDA)","issue":"1","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Food and Drug Analysis"],"abstract":"<jats:p>Gut microbiota produces a wide range of metabolites and plays a critical role in maintaining host health. Dysregulation of these metabolites can influence host metabolism through systemic circulation, contributing to the development of various diseases, including immunological, neurological and cancer-related disorders. Chromatography coupled with mass spectrometry (MS) has emerged as a powerful analytical approach, offering high sensitivity and resolution for studying gut microbiota-related metabolites. This review provides a comprehensive overview of chromatographic MS-based methods applied to the study of the gut microbial metabolome. We summarize strategies for sample collection, storage, and preparation of commonly analyzed sample types, including feces, plasma\/serum, urine, and tissue samples. In addition, we included the main chromatographic MS-based approaches, as well as data analysis techniques, for investigating the gut microbial metabolome. The characteristics and utility of liquid chromatographic-mass spectrometry (LC-MS), gas chromatographic-mass spectrometry (GC-MS), and capillary electrophoresis-mass spectrometry (CE-MS) were discussed in the context of providing broader coverage of gut microbiota-derived metabolites with diverse physicochemical properties. Finally, we summarize recent studies that have employed chromatographic MS-based approaches to investigate gut microbiota-related disease. Through the integration of appropriate sample handling and advanced analytical strategies, a deeper understanding of host\u2013microbiota interactions and their roles in health and disease can be achieved.<\/jats:p>","DOI":"10.38212\/2224-6614.3568","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T10:22:52Z","timestamp":1775211772000},"source":"Crossref","is-referenced-by-count":0,"title":["Mass spectrometry-based metabolomics for studying gut microbiota-derived metabolites in biological samples"],"prefix":"10.38212","volume":"34","author":[{"given":"Ya-Ting","family":"Lin","sequence":"first","affiliation":[{"name":"School of Pharmacy, National Taiwan University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huai-Hsuan","family":"Chiu","sequence":"additional","affiliation":[{"name":"Department of Medical Research, National Taiwan University Hospital, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Ning","family":"Cheng","sequence":"additional","affiliation":[{"name":"School of Pharmacy, National Taiwan University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-Hua","family":"Kuo","sequence":"additional","affiliation":[{"name":"The Metabolomics Core Laboratory, Center of Genomic and Precision Medicine, National Taiwan University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"25385","published-online":{"date-parts":[[2026,3,31]]},"container-title":["Journal of Food and Drug Analysis"],"original-title":[],"language":"en","deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:14:46Z","timestamp":1792584886000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jfda-online.com\/journal\/vol34\/iss1\/2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,31]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,3,31]]}},"URL":"https:\/\/doi.org\/10.38212\/2224-6614.3568","relation":{},"ISSN":["2224-6614"],"issn-type":[{"value":"2224-6614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,31]]}}}