{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:44:52Z","timestamp":1792586692051,"version":"4.5.0"},"reference-count":0,"publisher":"The Journal of Food and Drug Analysis (JFDA), Food and Drug Administration, Taiwan (TFDA)","issue":"1","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Food and Drug Analysis"],"abstract":"<jats:p>Oral squamous cell carcinoma (OSCC) remains a major global health challenge, and early detection is essential for improving patient outcomes. To identify reliable salivary biomarkers, we performed a targeted metabolomics study using 13C2\/12C2-dansylation, integrating candidate metabolites from published OSCC metabolomics datasets and systematically verifying them through LC-MRM-MS. A panel of 26 metabolites was quantified in saliva samples from 299 subjects, including healthy controls (n = 98), oral potentially malignant disorders (OPMD I, n = 50; OPMD II, n = 53), and OSCC patients (n = 98). Among these, cadaverine, N-acetylcadaverine, choline, glycine, and tryptophan were significantly dysregulated across disease groups. Cadaverine, N-acetylcadaverine and choline were consistently elevated in OSCC and showed progressive increases with disease stages, whereas glycine levels declined. A four-metabolite panel (cadaverine, N-acetylcadaverine, choline, glycine) demonstrated strong diagnostic performance in distinguishing OSCC from healthy controls (AUC = 0.91). Correlation analyses further revealed coordinated regulation among polyamine-related metabolites, suggesting a reprogramming of ornithine\u2013polyamine metabolism in OSCC progression. In conclusion, we establish a verified salivary metabolite panel with high discriminatory power for OSCC detection. These findings highlight polyamine-associated metabolic alterations as a hallmark of malignant transformation in the oral cavity and support salivary metabolomics as a clinically accessible tool for non-invasive screening and risk stratification.<\/jats:p>","DOI":"10.38212\/2224-6614.3586","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T08:17:37Z","timestamp":1783585057000},"source":"Crossref","is-referenced-by-count":0,"title":["Systematic verification of saliva-based biosignatures for oral cancer via targeted metabolomics"],"prefix":"10.38212","volume":"34","author":[{"given":"Ya-Ju","family":"Hsieh","sequence":"first","affiliation":[{"name":"Molecular Medicine Research Center, College of Medicine, Chang Gung University, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Chang","sequence":"additional","affiliation":[{"name":"Molecular Medicine Research Center, College of Medicine, Chang Gung University, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi","family":"Yang","sequence":"additional","affiliation":[{"name":"Molecular Medicine Research Center, College of Medicine, Chang Gung University, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nai Hsuan","family":"Teng","sequence":"additional","affiliation":[{"name":"Molecular Medicine Research Center, College of Medicine, Chang Gung University, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jau-Song","family":"Yu","sequence":"additional","affiliation":[{"name":"Molecular Medicine Research Center, College of Medicine, Chang Gung University, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai-Ping","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head & Neck Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Chen","family":"Chen","sequence":"additional","affiliation":[{"name":"Molecular Medicine Research Center, College of Medicine, Chang Gung University, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lichieh Julie","family":"Chu","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Kidney Research Center, Department of Nephrology, LinKou Chang Gung Memorial Hospital, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"25385","published-online":{"date-parts":[[2026,7,6]]},"container-title":["Journal of Food and Drug Analysis"],"original-title":[],"language":"en","deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:14:39Z","timestamp":1792584879000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jfda-online.com\/journal\/vol34\/iss1\/9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,6]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,7,6]]}},"URL":"https:\/\/doi.org\/10.38212\/2224-6614.3586","relation":{},"ISSN":["2224-6614"],"issn-type":[{"value":"2224-6614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,6]]}}}