{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:44:40Z","timestamp":1792586680843,"version":"4.5.0"},"reference-count":0,"publisher":"The Journal of Food and Drug Analysis (JFDA), Food and Drug Administration, Taiwan (TFDA)","issue":"3","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Food and Drug Analysis"],"abstract":"<jats:p>Aflatoxin B1 (AFB1) poses a severe threat to food safety due to its potent carcinogenicity. To address the need for rapid and on-site detection, this study developed a colorimetric biosensor based on an AFB1-responsive DNA aptamer-cross-linked sodium alginate hydrogel. Gold nanoparticles (AuNPs) were encapsulated within the hydrogel as a visual signal reporter. The sensing mechanism relies on target-induced dissociation of aptamer crosslinkers, leading to hydrogel disintegration and release of AuNPs. The hydrogel formulation was optimized regarding sodium alginate concentration and crosslinking conditions. To enhance sensitivity, an Exonuclease I (Exo I)-triggered target recycling strategy was implemented, which amplifies hydrogel network disruption by cleaving and recycling the aptamer. The sensor demonstrated a linear detection range of 0-50 \u00b5M, with a detection limit of 12.9 nM, and exhibited high selectivity towards AFB1. This work presents a sensitive, visually readable, and portable platform for mycotoxin detection in food safety applications.<\/jats:p>","DOI":"10.38212\/2224-6614.3608","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T08:15:12Z","timestamp":1789028112000},"source":"Crossref","is-referenced-by-count":0,"title":["A target-responsive and signal-amplified DNA hydrogel biosensor for visual and quantitative detection of Aflatoxin B1"],"prefix":"10.38212","volume":"34","author":[{"given":"Haojie","family":"Du","sequence":"first","affiliation":[{"name":"School of New Materials and Future Technologies, Beijing Technology and Business University, No.11 Fucheng Road, Haidian District, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyue","family":"Liu","sequence":"additional","affiliation":[{"name":"School of New Materials and Future Technologies, Beijing Technology and Business University, No.11 Fucheng Road, Haidian District, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongshuai","family":"Li","sequence":"additional","affiliation":[{"name":"School of New Materials and Future Technologies, Beijing Technology and Business University, No.11 Fucheng Road, Haidian District, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyi","family":"Wei","sequence":"additional","affiliation":[{"name":"School of New Materials and Future Technologies, Beijing Technology and Business University, No.11 Fucheng Road, Haidian District, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinning","family":"Wang","sequence":"additional","affiliation":[{"name":"School of New Materials and Future Technologies, Beijing Technology and Business University, No.11 Fucheng Road, Haidian District, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunxuan","family":"Weng","sequence":"additional","affiliation":[{"name":"School of New Materials and Future Technologies, Beijing Technology and Business University, No.11 Fucheng Road, Haidian District, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of New Materials and Future Technologies, Beijing Technology and Business University, No.11 Fucheng Road, Haidian District, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"25385","published-online":{"date-parts":[[2026,9,10]]},"container-title":["Journal of Food and Drug Analysis"],"original-title":[],"language":"en","deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:14:09Z","timestamp":1792584849000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jfda-online.com\/journal\/vol34\/iss3\/15"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,10]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,9,10]]}},"URL":"https:\/\/doi.org\/10.38212\/2224-6614.3608","relation":{},"ISSN":["2224-6614"],"issn-type":[{"value":"2224-6614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,10]]}}}