{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T18:02:29Z","timestamp":1784138549378,"version":"3.55.0"},"reference-count":31,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antivir Chem Chemother"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:sec><jats:title>Background:<\/jats:title><jats:p> Hepatitis C virus (HCV) infection is a major cause of chronic liver disease. Here, we report a new and effective strategy for inhibiting HCV replication using an inhibitor of heat-shock protein 90, 17-AAG (17-allylamino-17demethoxygeldanamycin), and a proteasome inhibitor, MG132. <\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p> To explore the virological basis of combination therapy, we analysed the effects of 17-AAG and MG132, singly and in combination on HCV replication in an HCV replicon cell system. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p> In HCV replicon cells, HCV RNA replication was suppressed by 17-AAG in a dose-dependent manner. As shown in the present study, the 50% inhibitory concentration values were 0.82 nM for 17-AAG and 0.21 nM for MG132. Low concentrations of MG132 had strong synergistic inhibitory effects with low toxicity on HCV replicon cells. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p> The results of this study suggest that the different effects and synergistic actions of 17-AAG and MG132 could provide a new therapeutic approach to HCV infection. <\/jats:p><\/jats:sec>","DOI":"10.3851\/imp1479","type":"journal-article","created":{"date-parts":[[2010,1,18]],"date-time":"2010-01-18T15:46:40Z","timestamp":1263829600000},"page":"161-167","source":"Crossref","is-referenced-by-count":5,"title":["Combination Therapy for Hepatitis C Virus with Heat-Shock Protein 90 Inhibitor 17-AAG and Proteasome Inhibitor MG132"],"prefix":"10.1177","volume":"20","author":[{"given":"Saneyuki","family":"Ujino","sequence":"first","affiliation":[{"name":"Department of Life and Environmental Sciences, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saori","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental Sciences, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunitada","family":"Shimotohno","sequence":"additional","affiliation":[{"name":"Research Institute, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba, Japan"},{"name":"Center of Integrated Medical Research, School of Medicine, Keio University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Takaku","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental Sciences, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba, Japan"},{"name":"Research Institute, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba, Japan"},{"name":"High Technology Research Center, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2010,4,1]]},"reference":[{"key":"bibr1-IMP1479","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198911303212202"},{"key":"bibr2-IMP1479","doi-asserted-by":"publisher","DOI":"10.1126\/science.2523562"},{"key":"bibr3-IMP1479","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i17.2461"},{"key":"bibr4-IMP1479","first-page":"635","volume":"2","author":"Witth\u00f6ft T","year":"2008","journal-title":"Biologics"},{"key":"bibr5-IMP1479","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.17.6547"},{"key":"bibr6-IMP1479","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510260704"},{"key":"bibr7-IMP1479","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2001.21747"},{"key":"bibr8-IMP1479","doi-asserted-by":"publisher","DOI":"10.1038\/35055162"},{"key":"bibr9-IMP1479","doi-asserted-by":"publisher","DOI":"10.1177\/153537020322800201"},{"key":"bibr10-IMP1479","doi-asserted-by":"publisher","DOI":"10.1007\/PL00012491"},{"key":"bibr11-IMP1479","doi-asserted-by":"publisher","DOI":"10.1007\/s10254-003-0021-1"},{"key":"bibr12-IMP1479","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205184"},{"key":"bibr13-IMP1479","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/91.22.1940"},{"key":"bibr14-IMP1479","first-page":"986","volume":"8","author":"Solit DB","year":"2002","journal-title":"Clin Cancer Res"},{"key":"bibr15-IMP1479","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M806452200"},{"key":"bibr16-IMP1479","doi-asserted-by":"publisher","DOI":"10.1007\/BF00689048"},{"key":"bibr17-IMP1479","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.38.23843"},{"key":"bibr18-IMP1479","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8617"},{"key":"bibr19-IMP1479","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.24.8357-8364.2001"},{"key":"bibr20-IMP1479","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2005.02.046"},{"key":"bibr21-IMP1479","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.9.4510-4520.2006"},{"key":"bibr22-IMP1479","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)00342-X"},{"key":"bibr23-IMP1479","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(99)70185-X"},{"key":"bibr24-IMP1479","doi-asserted-by":"publisher","DOI":"10.1086\/382595"},{"key":"bibr25-IMP1479","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.66.3.1476-1483.1992"},{"key":"bibr26-IMP1479","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2893.1999.00150.x"},{"key":"bibr27-IMP1479","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.2.1165-1174.1999"},{"key":"bibr28-IMP1479","first-page":"31","volume":"203","author":"Hellen CU","year":"1995","journal-title":"Curr Top Microbiol Immunol"},{"key":"bibr29-IMP1479","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.05.067"},{"key":"bibr30-IMP1479","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301032200"},{"key":"bibr31-IMP1479","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.19.10237-10249.2003"}],"container-title":["Antiviral Chemistry and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3851\/IMP1479","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.3851\/IMP1479","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3851\/IMP1479","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T11:23:24Z","timestamp":1725449004000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.3851\/IMP1479"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["10.3851\/IMP1479"],"URL":"https:\/\/doi.org\/10.3851\/imp1479","relation":{},"ISSN":["0956-3202","2040-2066"],"issn-type":[{"value":"0956-3202","type":"print"},{"value":"2040-2066","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4]]}}}