{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T08:48:46Z","timestamp":1777452526904,"version":"3.51.4"},"reference-count":14,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Antiviral Therapy"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Facial lipoatrophy, a common alteration among HIV-1-infected, antiretroviral-treated patients, is often corrected using autologous transplantation. In some cases, especially when enlarged adipose tissue from the dorso-cervical area (that is, a \u2018buffalo hump') is used as a source of fat for transplantation, the transplanted fat develops progressive hypertrophy. To gain insight into the molecular basis of this phenomenon, we evaluated the cell morphology and gene expression in this hypertrophied facial fat.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Quantitative real-time PCR was used to examine the expression of various marker genes in a sample of facial fat that underwent hypertrophy after autologous transplantation. The results were compared with gene expression data from \u2018buffalo hump\u2019 fat and subcutaneous fat from healthy controls. Optical and electron microscopic analyses were used to determine cell morphology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The enlarged facial adipose tissue did not exhibit the overt microscopic morphology of brown adipose tissue but (similar to \u2018buffalo hump\u2019 fat) it contained adipocytes heterogeneous in size. The enlarged facial fat retained the partial molecular signature of a distorted brown-to-white adipocyte phenotype, including expression of uncoupling protein-1 (UCP1) transcript, and showed unaltered adipogenesis and inflammation that are characteristic of \u2018buffalo hump\u2019 fat.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Despite being implanted in a former lipoatrophic area, facially grafted \u2018buffalo hump\u2019 tissue appears to retain the altered phenotype of dorso-cervical adipose cells, thus accounting for its progressive enlargement. These results argue that caution should be exercised when considering \u2018buffalo hump\u2019 fat depots as a fat source for autologous transplantation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3851\/imp2528","type":"journal-article","created":{"date-parts":[[2013,1,23]],"date-time":"2013-01-23T13:11:56Z","timestamp":1358946716000},"page":"1-5","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Hypertrophied Facial Fat in An HIV-1-Infected Patient after Autologous Transplantation from \u2018Buffalo Hump\u2019 Retains a Partial Brown-Fat-Like Molecular Signature"],"prefix":"10.1177","volume":"18","author":[{"given":"Jos\u00e9 M","family":"Gallego-Escuredo","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, and Institute of Biomedicine (IBUB), University of Barcelona, and CIBER Fisiopatologia de la Obesidad y Nutricion, Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pere","family":"Domingo","sequence":"additional","affiliation":[{"name":"Infectious Diseases Unit, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan","family":"Fontdevila","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, Hospital Clinic, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan","family":"Villarroya","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, and Institute of Biomedicine (IBUB), University of Barcelona, and CIBER Fisiopatologia de la Obesidad y Nutricion, Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo, Madrid, Spain"},{"name":"Infectious Diseases Unit, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan C","family":"Domingo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, and Institute of Biomedicine (IBUB), University of Barcelona, and CIBER Fisiopatologia de la Obesidad y Nutricion, Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esteban","family":"Martinez","sequence":"additional","affiliation":[{"name":"Hospital Clinic-IDIBAPS, University of Barcelona, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Giralt","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, and Institute of Biomedicine (IBUB), University of Barcelona, and CIBER Fisiopatologia de la Obesidad y Nutricion, Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesc","family":"Villarroya","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, and Institute of Biomedicine (IBUB), University of Barcelona, and CIBER Fisiopatologia de la Obesidad y Nutricion, Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,5,1]]},"reference":[{"key":"bibr1-IMP2528","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e32833f1463"},{"key":"bibr2-IMP2528","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e3282f56b40"},{"key":"bibr3-IMP2528","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.07.049"},{"key":"bibr4-IMP2528","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2011-2951"},{"key":"bibr5-IMP2528","doi-asserted-by":"publisher","DOI":"10.1086\/426686"},{"key":"bibr6-IMP2528","doi-asserted-by":"publisher","DOI":"10.1186\/1752-1947-2-284"},{"key":"bibr7-IMP2528","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2006.03.001"},{"key":"bibr8-IMP2528","doi-asserted-by":"publisher","DOI":"10.1042\/cs0750021"},{"key":"bibr9-IMP2528","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20120030"},{"key":"bibr10-IMP2528","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2007.06.001"},{"key":"bibr11-IMP2528","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1177\/135965350601100610","volume":"11","author":"Giralt M.","year":"2006","journal-title":"Antivir Ther"},{"key":"bibr12-IMP2528","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00015.2003"},{"key":"bibr13-IMP2528","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0808949"},{"key":"bibr14-IMP2528","doi-asserted-by":"publisher","DOI":"10.2337\/db11-0075"}],"container-title":["Antiviral 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