{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T08:55:06Z","timestamp":1777452906815,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Antiviral Therapy"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Although the World Health Organization has declared the eradication of smallpox in 1980, the fear of its potential use in bioterrorism has become a reality. Since the effectiveness of current vaccines and antiviral drugs is limited, development of new therapeutic strategies is needed. In this study, we investigated small interfering RNA (siRNA) as a therapeutic approach for preventing and treating smallpox infection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Eight siRNA sequences were designed and evaluated for antiviral activity against vaccinia virus (VACV) in vitro and in vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Of eight siRNAs, A5R1 siRNA targeted the A5R gene and reduced VACV replication in cell culture by up to 85% at 100 nM concentration without inducing cytotoxicity. A prolonged prophylactic as well as therapeutic effect of siRNA was observed. In addition, real-time PCR analysis showed that A5R1 siRNA can especially reduce the target mRNA. Finally, intraperitoneal delivery of A5R1 siRNA in Balb\/c mice significantly protected these animals from lethal challenge with VACV.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study suggests the potential of A5R1 siRNA as a therapeutic antiviral agent against smallpox.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3851\/imp3022","type":"journal-article","created":{"date-parts":[[2016,1,7]],"date-time":"2016-01-07T09:00:33Z","timestamp":1452157233000},"page":"397-404","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Protection against Lethal Vaccinia Virus Infection in Mice using an siRNA Targeting the A5R Gene"],"prefix":"10.1177","volume":"21","author":[{"given":"Dongjoon","family":"Seo","sequence":"first","affiliation":[{"name":"Department of Life Science, Sogang University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na Young","family":"Kim","sequence":"additional","affiliation":[{"name":"ABION Inc., Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin Ah","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Life Science, Sogang University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kang Rok","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Life Science, Sogang University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gyeung Haeng","family":"Hur","sequence":"additional","affiliation":[{"name":"Agency for Defense Development, Daejeon, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jai Myung","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Life Science, Sogang University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sungho","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Life Science, Sogang University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,7,1]]},"reference":[{"key":"bibr1-IMP3022","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68143-9"},{"key":"bibr2-IMP3022","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.07.103"},{"key":"bibr3-IMP3022","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12536"},{"key":"bibr4-IMP3022","doi-asserted-by":"publisher","DOI":"10.4161\/hv.10295"},{"key":"bibr5-IMP3022","doi-asserted-by":"publisher","DOI":"10.2217\/fvl.11.27"},{"key":"bibr6-IMP3022","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00977-13"},{"key":"bibr7-IMP3022","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2966"},{"key":"bibr8-IMP3022","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2310"},{"key":"bibr9-IMP3022","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2009.05.002"},{"key":"bibr10-IMP3022","first-page":"52","volume":"8","author":"Nekhai 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