{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T05:44:31Z","timestamp":1790228671091,"version":"4.1.0"},"reference-count":51,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Antiviral Therapy"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cacicol\u00ae, a topical eye biopolymer containing a poly-carboxymethylglucose sulfate solution that is a regenerating matrix therapy agent, intended for wound healing of persistent corneal epithelial defects. Based on the chemical composition, we hypothesized that Cacicol\u00ae may compete with natural heparan sulfate (HS) which initiates cell surface attachment of herpes simplex virus type-1 (HSV-1), varicella zoster virus (VZV) and human adenovirus (HAdV), three viruses associated with corneal infections.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Cacicol\u00ae was compared to vehicle in the following viral strains: HSV-1 SC16 strain and HSV-1 PSLR, a clinical isolate highly resistant to acyclovir and foscarnet; VZV ATH and VZV FLO, two VZV clinical isolates; and HAdV-D37 strain. Viruses in Cacicol\u00ae or vehicle were added to cells for 1 h during adsorption then viral replication was assessed by plaque reduction assays on Vero cells for HSV-1 and MeWo cells for VZV and by immunostaining assay on Hep-2 cells for HAdV-D37.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The vehicle had no effect, dose-dependent effects were demonstrated when HSV-1 SC16, HSV-1 PSLR, VZV ATH and VZV FLO were inoculated in the presence of Cacicol\u00ae, inhibiting viral replication by 98.4%, 98.9%, 90.1% and 89.0%, respectively. Cacicol\u00ae had no antiviral effect against HAdV-D37.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Cacicol\u00ae has a significant antiviral activity on HSV-1 and VZV, but not on HAdV-D37. The lack of effect on HAdV is probably because it is less dependent on HS interactions for cell entry. Clinical studies are necessary to determine Cacicol\u00ae for an adjunct or alternative therapy of corneal HSV-1 or VZV infection, particularly for the management of antiviral resistant HSV-1.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3851\/imp3254","type":"journal-article","created":{"date-parts":[[2018,7,20]],"date-time":"2018-07-20T10:25:17Z","timestamp":1532082317000},"page":"665-675","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Antiviral effects of Cacicol\u00ae, a heparan sulfate biomimetic for corneal regeneration therapy, for herpes simplex virus type-1 and varicella zoster virus infection"],"prefix":"10.1177","volume":"23","author":[{"given":"Claire","family":"Deback","sequence":"first","affiliation":[{"name":"Department of Virology, Paul Brousse Hospital, Assistance Publique-H\u00f4pitaux de Paris, Villejuif, France"},{"name":"Inflammation Chimiokines et Immunopathologie, INSERM 996, Fac. de m\u00e9decine - Univ.Paris-Sud, Universit\u00e9 Paris-Saclay, Clamart, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Rousseau","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Bic\u00eatre Hospital, Assistance Publique-H\u00f4pitaux de Paris, Univ.Paris-Sud, Kremlin-Bic\u00eatre, France"},{"name":"Center for Immunology of Viral Infections and Autoimmune Diseases, IDMIT Infrastructure, CEA, Univ.Paris-Sud, Inserm U1184, Fontenay-aux-Roses Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magali","family":"Breckler","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Bic\u00eatre Hospital, Assistance Publique-H\u00f4pitaux de Paris, Univ.Paris-Sud, Kremlin-Bic\u00eatre, France"},{"name":"Center for Immunology of Viral Infections and Autoimmune Diseases, IDMIT Infrastructure, CEA, Univ.Paris-Sud, Inserm U1184, Fontenay-aux-Roses Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Molet","sequence":"additional","affiliation":[{"name":"Department of Virology, Paul Brousse Hospital, Assistance Publique-H\u00f4pitaux de Paris, Villejuif, France"},{"name":"Inflammation Chimiokines et Immunopathologie, INSERM 996, Fac. de m\u00e9decine - Univ.Paris-Sud, Universit\u00e9 Paris-Saclay, Clamart, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Boutolleau","sequence":"additional","affiliation":[{"name":"AP-HP, H\u00f4pitaux Universitaires La Piti\u00e9 Salp\u00eatri\u00e8re - Charles Foix, Service de Virologie, and Sorbonne Universit\u00e9s, UPMC Univ Paris 06, CR7, CIMI, INSERM U1135, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Burrel","sequence":"additional","affiliation":[{"name":"AP-HP, H\u00f4pitaux Universitaires La Piti\u00e9 Salp\u00eatri\u00e8re - Charles Foix, Service de Virologie, and Sorbonne Universit\u00e9s, UPMC Univ Paris 06, CR7, CIMI, INSERM U1135, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Marie","family":"Roque-Afonso","sequence":"additional","affiliation":[{"name":"Department of Virology, Paul Brousse Hospital, Assistance Publique-H\u00f4pitaux de Paris, INSERM1193 - Fac. de m\u00e9decine - Univ Paris-Sud, Universit\u00e9 Paris-Saclay, Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Labetoulle","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Bic\u00eatre Hospital, Assistance Publique-H\u00f4pitaux de Paris, Univ.Paris-Sud, Kremlin-Bic\u00eatre, France"},{"name":"Center for Immunology of Viral Infections and Autoimmune Diseases, IDMIT Infrastructure, CEA, Univ.Paris-Sud, Inserm U1184, Fontenay-aux-Roses Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,11,1]]},"reference":[{"key":"bibr1-IMP3254","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-3729"},{"key":"bibr2-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2004.11.052"},{"key":"bibr3-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2012.01.005"},{"key":"bibr4-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2007.10.009"},{"key":"bibr5-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2007.10.010"},{"key":"bibr6-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2015.04.001"},{"key":"bibr7-IMP3254","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.24.15265-15276.2005"},{"key":"bibr8-IMP3254","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12-9656"},{"key":"bibr9-IMP3254","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01010-16"},{"key":"bibr10-IMP3254","doi-asserted-by":"publisher","DOI":"10.1086\/590668"},{"key":"bibr11-IMP3254","doi-asserted-by":"publisher","DOI":"10.1002\/med.1026"},{"key":"bibr12-IMP3254","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00296-06"},{"key":"bibr13-IMP3254","doi-asserted-by":"publisher","DOI":"10.1038\/348344a0"},{"key":"bibr14-IMP3254","doi-asserted-by":"publisher","DOI":"10.1126\/science.2162560"},{"key":"bibr15-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2013.01.015"},{"key":"bibr16-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(97)00149-4"},{"key":"bibr17-IMP3254","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1999.0171"},{"key":"bibr18-IMP3254","doi-asserted-by":"publisher","DOI":"10.1111\/aos.13116"},{"key":"bibr19-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfo.2013.01.012"},{"key":"bibr20-IMP3254","first-page":"457","volume":"29","author":"Cejkova J.","year":"2014","journal-title":"Histol Histopathol"},{"key":"bibr21-IMP3254","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/S0181-5512(08)72462-8","volume":"31","author":"Chebbi C.K.","year":"2008","journal-title":"J Fr Ophtalmol"},{"key":"bibr22-IMP3254","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12-10476"},{"key":"bibr23-IMP3254","doi-asserted-by":"crossref","DOI":"10.1016\/j.jfo.2011.09.002","volume":"35","author":"De Monchy I.","year":"2012","journal-title":"J Fr Ophtalmol"},{"key":"bibr24-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2005.11.004"},{"key":"bibr25-IMP3254","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050364"},{"key":"bibr26-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/0169-2607(95)01703-8"},{"key":"bibr27-IMP3254","doi-asserted-by":"publisher","DOI":"10.1086\/606028"},{"key":"bibr28-IMP3254","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit350"},{"key":"bibr29-IMP3254","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-481X.2010.00748.x"},{"key":"bibr30-IMP3254","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.67.1.93-100.1993"},{"key":"bibr31-IMP3254","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1995.1184"},{"key":"bibr32-IMP3254","first-page":"2711","volume":"18","author":"Shukla N.D.","year":"2012","journal-title":"Mol Vis"},{"key":"bibr33-IMP3254","first-page":"129","volume":"342","author":"Arvin A.M.","year":"2010","journal-title":"Curr Top Microbiol Immunol"},{"key":"bibr34-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.11.007"},{"key":"bibr35-IMP3254","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.635508"},{"key":"bibr36-IMP3254","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-75-11-3087"},{"key":"bibr37-IMP3254","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-79-3-549"},{"key":"bibr38-IMP3254","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7985"},{"key":"bibr39-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.06.041"},{"key":"bibr40-IMP3254","doi-asserted-by":"publisher","DOI":"10.1080\/02713680601038824"},{"key":"bibr41-IMP3254","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.201103"},{"key":"bibr42-IMP3254","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0126239"},{"key":"bibr43-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2015.01.008"},{"key":"bibr44-IMP3254","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01939-15"},{"key":"bibr45-IMP3254","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-84-20714"},{"key":"bibr46-IMP3254","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.8.3897-3905.2004"},{"key":"bibr47-IMP3254","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.14-15635"},{"key":"bibr48-IMP3254","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.18.8772-8780.2001"},{"key":"bibr49-IMP3254","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000189"},{"key":"bibr50-IMP3254","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2267"},{"key":"bibr51-IMP3254","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2016.05.004"}],"container-title":["Antiviral Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3851\/IMP3254","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.3851\/IMP3254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:52:36Z","timestamp":1777380756000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.3851\/IMP3254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,1]]},"references-count":51,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.3851\/IMP3254"],"URL":"https:\/\/doi.org\/10.3851\/imp3254","relation":{},"ISSN":["1359-6535","2040-2058"],"issn-type":[{"value":"1359-6535","type":"print"},{"value":"2040-2058","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,1]]}}}