{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:12:12Z","timestamp":1790194332968,"version":"4.1.0"},"reference-count":0,"publisher":"Journal of Infection in Developing Countries","issue":"04","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Infect Dev Ctries"],"abstract":"<jats:p>Introduction: The spread of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-En) from turkeys via food chain and environmental contamination is a human health concern.\nMethodology: Seventy fecal samples were collected from healthy turkeys and streaked on Tryptone Bile X-Glucoronide (TBX) supplemented with 2 mg\/L of cefotaxime and on TBX supplemented with 1 mg\/L of imipenem. ESBL production and susceptibility to antibiotics were studied according to CLSI guidelines. Genes encoding for ESBLs (SHV, CTX-M, TEM), carbapenemases (IMI, KPC, OXA48, NDM), tetracyclines (tetA, tetB, tetC), colistin (mcr-1 to mcr-5), sulphonamides (sul1, sul2), quinolones (qnr A\/B\/S, aac(6\u2019)-Ib-cr, qepA) resistance, and class 1 and 2 integrons were determined by PCR.\nResults: ESBL-En [n = 45 (64.3%): 41 E. coli and 4 K. pneumoniae] isolates were collected. In E. coli, blaCTX-M-1, blaCTX-M-14, blaCTX-M-15, blaCTX-M-27, and blaCTX-M-55 genes were identified in 23, 2, 5, 16, and one isolate, respectively. The blaCTX-M-15 gene was detected in two K. pneumoniae isolates, while each of blaCTX-M-1 and blaCTX-M-27 were detected in one isolate. Resistances to tetracyclines, sulfonamides, fluoroquinolones, and colistin were encoded by tetA (n = 21)\/tetB (n = 1), sul1 (n = 8)\/sul2 (n = 13), aac(6\u2019)-Ib-cr (n = 6), and mcr-1 (n = 2)\/mcr-2 (n = 1) genes, respectively. Integrons of class 1 and class 2 were detected in 15 and six isolates, respectively. Five E. coli isolates belonged to the pandemic ST131 clone.\nConclusions: Our findings highlight the high occurrence of MDR\/ESBL-En and demonstrate the possible transfer of these strains to humans via the food chain or direct contact.<\/jats:p>","DOI":"10.3855\/jidc.18973","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T17:58:46Z","timestamp":1745949526000},"page":"560-568","source":"Crossref","is-referenced-by-count":3,"title":["Genetic characterization of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae collected from healthy turkeys"],"prefix":"10.3855","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5590-5089","authenticated-orcid":false,"given":"Amel","family":"Romdhani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0595-2591","authenticated-orcid":false,"given":"Sana","family":"Dhaouadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4163-6023","authenticated-orcid":false,"given":"Sarah","family":"Cheriet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Lengliz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6590-2406","authenticated-orcid":false,"given":"Ilhem","family":"Boutiba-Ben Boubaker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4161-9569","authenticated-orcid":false,"given":"Ramzi Boubaker","family":"Landolsi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9673-193X","authenticated-orcid":false,"given":"Mohamed Salah","family":"Abbassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2222","published-online":{"date-parts":[[2025,4,29]]},"container-title":["The Journal of Infection in Developing Countries"],"original-title":[],"link":[{"URL":"https:\/\/www.jidc.org\/index.php\/journal\/article\/download\/18973\/3632","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.jidc.org\/index.php\/journal\/article\/download\/18973\/3632","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T17:58:46Z","timestamp":1745949526000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jidc.org\/index.php\/journal\/article\/view\/18973"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,29]]},"references-count":0,"journal-issue":{"issue":"04","published-online":{"date-parts":[[2025,4,29]]}},"URL":"https:\/\/doi.org\/10.3855\/jidc.18973","relation":{},"ISSN":["1972-2680"],"issn-type":[{"value":"1972-2680","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,29]]}}}