{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:31:56Z","timestamp":1759336316504,"version":"3.37.3"},"reference-count":0,"publisher":"Journal of Infection in Developing Countries","issue":"08","license":[{"start":{"date-parts":[[2024,8,31]],"date-time":"2024-08-31T00:00:00Z","timestamp":1725062400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["N35A640044"],"award-info":[{"award-number":["N35A640044"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Infect Dev Ctries"],"abstract":"<jats:p>Introduction: COVID-19, an emerging infectious disease caused by SARS-CoV-2, continues to be a global public health threat. The development of a colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) can extend the availability of simple, reliable molecular tests for the rapid detection of COVID-19.\nMethodology: The RT-LAMP assay was developed using a new primer set targeting a portion of SARS-CoV-2 orf8. The method was validated at 63 \u00baC for 60 minutes with naked-eye visualization of the color change. The clinical performance was compared to a real-time reverse transcription-polymerase chain reaction (rtRT-PCR) using 273 RNA samples extracted from nasopharyngeal swab specimens.\nResults: The developed RT-LAMP was specific to SARS-CoV-2 with a limit of detection at 15 RNA copies per reaction. The assay demonstrated diagnostic accuracy, sensitivity, specificity, positive predictive value, and negative predictive value of 90.48% (95% CI: 86.36\u201393.68%), 87.00% (95% CI: 81.53\u201391.33%), 100% (95% CI: 95.07\u2013100%), 100% (95% CI: not available), and 73.74% (95% CI: 66.22\u201380.07%), respectively, compared to the rtRT-PCR. The greatest sensitivity of 98.03% (95% CI: 94.34\u201399.59%) was demonstrated in samples with the cycle threshold (Ct) values &lt; 30 cycles.\nConclusions: The RT-LAMP method in this study showed good performance. The assay can increase the scope of laboratory testing for rapidly detecting SARS-CoV-2 in Thailand. Due to a decrease in COVID-19 cases, its application is beneficial when commercial alternatives are unavailable.<\/jats:p>","DOI":"10.3855\/jidc.19442","type":"journal-article","created":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T19:22:10Z","timestamp":1726600930000},"page":"1196-1203","source":"Crossref","is-referenced-by-count":3,"title":["An alternative colorimetric RT-LAMP assay for the rapid detection of SARS-CoV-2: development and validation in Thailand"],"prefix":"10.3855","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4396-0410","authenticated-orcid":false,"given":"Wiphat","family":"Klayut","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0765-7295","authenticated-orcid":false,"given":"Thanee","family":"Wongchai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0267-7993","authenticated-orcid":false,"given":"Nattagarn","family":"Chuenchom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5486-3631","authenticated-orcid":false,"given":"Payu","family":"Bhakdeenuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1454-5116","authenticated-orcid":false,"given":"Supranee","family":"Bunchoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8589-2309","authenticated-orcid":false,"given":"Sopa","family":"Srisungngam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5263-2743","authenticated-orcid":false,"given":"Sirilada","family":"Suphankong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6507-2898","authenticated-orcid":false,"given":"Benjawan","family":"Phetsuksiri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1879-0618","authenticated-orcid":false,"given":"Ballang","family":"Uppapong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4859-7243","authenticated-orcid":false,"given":"Janisara","family":"Rudeeaneksin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2222","published-online":{"date-parts":[[2024,8,31]]},"container-title":["The Journal of Infection in Developing Countries"],"original-title":[],"link":[{"URL":"https:\/\/www.jidc.org\/index.php\/journal\/article\/download\/19442\/3413","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.jidc.org\/index.php\/journal\/article\/download\/19442\/3413","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T19:22:11Z","timestamp":1726600931000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jidc.org\/index.php\/journal\/article\/view\/19442"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,31]]},"references-count":0,"journal-issue":{"issue":"08","published-online":{"date-parts":[[2024,8,31]]}},"URL":"https:\/\/doi.org\/10.3855\/jidc.19442","relation":{},"ISSN":["1972-2680"],"issn-type":[{"type":"electronic","value":"1972-2680"}],"subject":[],"published":{"date-parts":[[2024,8,31]]}}}