{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T19:38:10Z","timestamp":1783885090986,"version":"3.55.0"},"reference-count":0,"publisher":"Journal of Infection in Developing Countries","issue":"05","license":[{"start":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T00:00:00Z","timestamp":1748649600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Infect Dev Ctries"],"abstract":"<jats:p>Introduction: Pseudomonas aeruginosa is a common nosocomial pathogen with multidrug resistance (MDR) and virulence factors (VFs). This study assessed the VFs and their associations with MDR and non-MDR isolates.\nMethodology: One hundred clinical isolates were analyzed for 12 VFs, encoding genes, and phenotypic traits. Antibiotic resistance patterns and correlations between VFs and MDR were investigated.\nResults: Aztreonam showed the highest resistance rate among MDR (94.7%) and ceftazidime showed the highest resistance rate among non-MDR isolates (44.2%). Carbapenems demonstrated the greatest susceptibility. VF positivity rates included 91% for algD, 90% for lasB, 86% for toxA, 82% for exoS, 19% for exoU, 78% for aprA, 75% for plcH, 94% for pigment production, 93% for biofilm formation, 72% for hemolysin, 65% for lipase, and 36% for DNase. Strong biofilm formation correlated with algD and lasB (93%). Pigment production was linked with lasB and toxA (94%). Strong biofilm formation was significantly higher in MDR isolates and resistant strains, than non-MDR isolates. No significant differences in VFs were observed between susceptible and resistant strains for lasB, algD, toxA, plcH, exoU, or general biofilm production; except for strong biofilm formation. Certain VFs correlated with susceptible isolates: exoS with tobramycin, aprA with aztreonam and piperacillin-tazobactam, pigment production with imipenem, DNase with aztreonam and norfloxacin, and lipase with tobramycin and ceftazidime.\nConclusions: P. aeruginosa isolates displayed diverse VFs, biofilm-forming abilities, and MDR profiles; with strong biofilm formation closely linked to MDR. Targeting biofilm-related genes (algD, lasB) could offer effective therapeutic interventions, helping mitigate MDR infections and improve clinical outcomes.<\/jats:p>","DOI":"10.3855\/jidc.20957","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T07:38:24Z","timestamp":1748849904000},"page":"712-722","source":"Crossref","is-referenced-by-count":4,"title":["Phenotypic and molecular analysis of Pseudomonas aeruginosa virulence and association with antibiotic resistance in Egypt"],"prefix":"10.3855","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1041-6872","authenticated-orcid":false,"given":"Salah AI","family":"Ali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya A","family":"Ghamry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal M","family":"Soliman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah M","family":"Abdo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6928-9170","authenticated-orcid":false,"given":"Nagwan G","family":"El-Menofy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manal M","family":"Al-Gerby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haytham K","family":"Mahrous","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2222","published-online":{"date-parts":[[2025,5,31]]},"container-title":["The Journal of Infection in Developing Countries"],"original-title":[],"link":[{"URL":"https:\/\/www.jidc.org\/index.php\/journal\/article\/download\/20957\/3652","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.jidc.org\/index.php\/journal\/article\/download\/20957\/3652","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T07:38:25Z","timestamp":1748849905000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jidc.org\/index.php\/journal\/article\/view\/20957"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,31]]},"references-count":0,"journal-issue":{"issue":"05","published-online":{"date-parts":[[2025,5,31]]}},"URL":"https:\/\/doi.org\/10.3855\/jidc.20957","relation":{},"ISSN":["1972-2680"],"issn-type":[{"value":"1972-2680","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,31]]}}}