{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T08:59:13Z","timestamp":1762505953936},"reference-count":0,"publisher":"Journal of Infection in Developing Countries","issue":"06","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Infect Dev Ctries"],"abstract":"<jats:p>Introduction: Diagnosis of brucellosis in Syria is based on the presence of compatible symptoms in addition to positive agglutination results. This study investigated the potential of culture, ELISA and real-time PCR to support the diagnosis in different clinical manifestations of brucellosis. Methodology: Peripheral blood samples from 34 suspected brucellosis patients and 42 probable chronic or relapsed brucellosis patients were tested by agglutination tests, culture, ELISA and real-time PCR. Results: Among 34 samples collected from suspected cases, 18\/34 (53%) were agglutination tests positive, 12\/34 (35%) were culture positive, 12\/34 (35%) were Brucella IgG positive, and 10\/34 (29%) were real-time PCR positive. Three out of 34 patients were positive by real-time PCR but not by agglutination tests or culture. Among 42 samples obtained from probable chronic or relapsed patients, 27\/42 (64%) were agglutination tests positive, 26\/42 (62%) were Brucella IgG positive, 4\/42 (10%) were culture positive, and 1\/42 (2%) was real-time PCR positive. Conclusion: To rule in or rule out the diagnosis of brucellosis, a combination of several tests should be applied. Agglutination tests should be performed first considering their high sensitivity. If the agglutination test is negative, real-time PCR, and\/or ELISA, and\/or culture should be performed. When relapse or chronic brucellosis are suspected, agglutination tests and\/or ELISA are recommended.<\/jats:p>","DOI":"10.3855\/jidc.2453","type":"journal-article","created":{"date-parts":[[2012,6,17]],"date-time":"2012-06-17T17:17:31Z","timestamp":1339953451000},"page":"495-500","source":"Crossref","is-referenced-by-count":11,"title":["Brucellosis laboratory tests in Syria: what are their diagnostic efficacies in different clinical manifestations?"],"prefix":"10.3855","volume":"6","author":[{"given":"Yara","family":"Alsayed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fawza","family":"Monem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2222","published-online":{"date-parts":[[2012,5,3]]},"container-title":["The Journal of Infection in Developing Countries"],"original-title":[],"link":[{"URL":"https:\/\/jidc.org\/index.php\/journal\/article\/download\/22706192\/738","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/jidc.org\/index.php\/journal\/article\/download\/22706192\/738","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,7,14]],"date-time":"2019-07-14T00:25:55Z","timestamp":1563063955000},"score":1,"resource":{"primary":{"URL":"https:\/\/jidc.org\/index.php\/journal\/article\/view\/22706192"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,5,3]]},"references-count":0,"journal-issue":{"issue":"06","published-online":{"date-parts":[[2012,6,15]]}},"URL":"https:\/\/doi.org\/10.3855\/jidc.2453","relation":{},"ISSN":["1972-2680"],"issn-type":[{"value":"1972-2680","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,5,3]]}}}