{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T13:50:18Z","timestamp":1686837018110},"reference-count":0,"publisher":"Journal of Infection in Developing Countries","issue":"05","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Infect Dev Ctries"],"abstract":"<jats:p>Background: Interleukin (IL)-12 is a potential adjuvant in a variety of diseases including schistosomiasis. The clinical use of IL-12, however, is limited by the toxicity associated with its systemic administration. We have developed a novel delivery system (designated F2 gel matrix) composed of poly-N-acetyl glucosamine that has the dual properties of sustaining the release of proteins (e.g. interleukins) and adjuvant effects. The main aim of this study was to use a mouse model to test whether IL-12 released from F2 gel can induce adjuvant effects in the schistosomiasis setting as compared to those obtained after systemic delivery of IL-12. Methodology: First, we compared the toxicity induced by paracrine (delivered by F2 gel) and systemic IL-12. Second, we compared the induction of cytokines induced by paracrine and systemic IL-12. Third, we compared the adjuvant effects of paracrine and systemic IL-12-based prophylactic vaccination against schistosomiasis using soluble worm antigen preparation (SWAP). Results: \u00a0IL-12 released from F2 gel did not induce significant toxicity measured by alanine aminotransferase (ALT). We found similar serum levels of IFN-\u03b3, TNF-\u03b1 and IL-2 after paracrine and systemic IL-12 treatments. We also found that vaccination with F2 gel\/SWAP\/IL-12 induced higher anti-schistosomal effects than IL-12\/SWAP as evidenced by 1) the decrease in the total liver egg counts; 2) the reduction in the granuloma size and fibrotic reaction in the liver; and 3) the amelioration of the liver functions. Conclusion: Collectively, these results indicate that IL-12-F2 gel delivery approach could be considered as a potential strategy for the treatment of schistosomiasis.<\/jats:p>","DOI":"10.3855\/jidc.541","type":"journal-article","created":{"date-parts":[[2010,6,10]],"date-time":"2010-06-10T10:39:18Z","timestamp":1276166358000},"page":"318-328","source":"Crossref","is-referenced-by-count":4,"title":["Using poly-N-acetyl glucosamine gel matrix to deliver IL-12 with anti-schistosomasis vaccination"],"prefix":"10.3855","volume":"4","author":[{"given":"Mohamed Labib","family":"Salem","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wael Y","family":"Attia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yousry E","family":"Al-Bolkiny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ismail M","family":"Al-Sharkawi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Demcheva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Vournakis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2222","published-online":{"date-parts":[[2010,5,9]]},"container-title":["The Journal of Infection in Developing Countries"],"original-title":[],"link":[{"URL":"https:\/\/jidc.org\/index.php\/journal\/article\/download\/20539064\/392","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/jidc.org\/index.php\/journal\/article\/download\/20539064\/392","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,7,22]],"date-time":"2019-07-22T00:21:34Z","timestamp":1563754894000},"score":1,"resource":{"primary":{"URL":"https:\/\/jidc.org\/index.php\/journal\/article\/view\/20539064"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5,9]]},"references-count":0,"journal-issue":{"issue":"05","published-online":{"date-parts":[[2010,6,5]]}},"URL":"https:\/\/doi.org\/10.3855\/jidc.541","relation":{},"ISSN":["1972-2680"],"issn-type":[{"value":"1972-2680","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,5,9]]}}}